Phytogenic compounds and bioactive substances for rabbits: benefits on reproduction, well-being and meat quality




Antioxidants; Natural Additives; Rabbit Farming.


The use of phytogenic additives in animal feed emerged with the need to replace traditional additives and growth promoters, which were banned for use by the European Union a few years ago and in Brazil recently. Since then, several works have been carried out for several species of animal production, so that currently the most used phytogenic additives are essential oils, extracts, herbs, spices and vegetable fractions that include bark, leaves, roots, seeds and others. These additives are composed of several bioactive substances of varied chemical nature, exerting positive functions on the animals' organism. In rabbit farming, they are mainly used to improve the functioning of the digestive system, reduce sanitary challenge and promote greater nutrient digestibility. In addition to improving nutrition, consumption, stimulating digestion and performance, the use of bioactive substances reduces the incidence of diseases, improves reproductive parameters, acts as antifungals, anthelmintics, antivirals, antimicrobials, anticoccidials and antioxidants. Due to these benefits, the current review addresses different types of phytogenic compounds and bioactive substances used in rabbit production, focusing on reproduction, welfare and meat quality.


Abdelnour, S. A., Abd El‐Hack, M. E., Alagawany, M., Taha, A. E., Elnesr, S. S., Abd Elmonem, O. M. & Swelum, A. A. (2020a). Useful impacts of royal jelly on reproductive sides, fertility rate and sperm traits of animals. Journal of Animal Physiology and Animal Nutrition, 104 (6), 1798-1808.

Abdelnour, S. A., Swelum, A. A., Salama, A., Al-Ghadi, M. Q., Qattan, S. Y. A., Abd El-Hack, M. E., Asmaa, F., Khafaga, A. R., Alhimaidi, B. O., Ammari, A. A. & El-Saadony, M. T. (2020b). The beneficial impacts of dietary phycocyanin supplementation on growing rabbits under high ambient temperature. Italian Journal of Animal Science, 19(1), 1046–1056.

Agarwal, A., Nallella, K. P., Allamaneni, S. R. & Said, T. M. (2004). Role of antioxidants in treatment of male infertility: an overview of the literature. Reproductive BioMedicine, 8, 616-627.

Ahmed, A. E., Alkahtani, M. A., Aboubaker, A. & Abdel-Wareth, A-M. (2020). Thyme leaves as an eco-friendly feed additive improves both the productive and reproductive performance of rabbits under hot climatic conditions. Veterinární Medícina, 65(12), 553–563.

Alagawany, M., Elnesr, S. S., Farag, M. R., Abd El-Hack, M. E., Khafaga, A. F., Taha, A. E., Tiwari, R., Yatoo, M. I., Bhatt, P., Gopi, M. & Kuldeep, D. (2019). Use of Licorice (Glycyrrhiza glabra) Herb as a Feed Additive in Poultry: Current Knowledge and Prospects. Animals, 9(8), 536-546.

Ali, F. H. M., Hassan, A. R. H. A. & Bahgat, A. E. M. (2015). Study of sumac extract (Rhus coriaria L.), lactic acid and thyme oil as decontaminants for shelf life extension of refrigerated rabbit meat. Food Science and Quality Management, 38, 71-78.

Angelo, P. M. & Jorge, N. (2007). Compostos fenólicos em alimentos – uma revisão. Revista do Instituto Adolfo Lutz, 66(1), 1-9.

Arroyo-Lopez, C., Manolaraki, F., Saratsis, A., Saratsi, K., Stefanakis, A., Skampardonis, V., Voutzourakis, N., Hoste, H. & Sotiraki, S. (2014). Anthelmintic effect of carob pods and sainfoin hay when fed to lambs after experimental trickle infections with Haemonchus contortus and Trichostrongylus colubriformis. PUBMED, 21, 1-9.

Bansal, A. K. & Bilaspuri, G. S. (2011). Impacts of oxidative stress and antioxidants on semen functions. Veterinary Medicine International, 1-7.

Bazzano, L. A., Serdula, M. K. & Liu, S. (2003). Dietary intake of fruits and vegetables and risk of cardiovascular disease. Current Atherosclerosis Reports, 5, 492–499.

Bezerra, H. V. A. (2019). Lignina purificada na dieta de ruminantes: impacto no desempenho e saúde de ovinos. 75 f. Dissertação (Mestrado) - Curso de Zootecnia, Universidade de São Paulo, Pirassununga.

Bhaskar, M. P. S., Kumar, K. S., Krishnan, M. P. K. & Antony, H. (2013). Quercetin alleviates hypercholesterolemic diet induced inflammation during progression and regression of atherosclerosis in rabbits. Nutrition, 29, 219–229

Bogacz-Radomska, L. & Harasym, J. (2018). β-Carotene—properties and production methods. Food Quality and Safety, 2(2), 1–6.

Brasil. Ministério da Agricultura, Pecuária e Abastecimento. Mapa. Instrução Normativa Mapa nº 13, de 01 de dezembro de 2004, sobre a regulamentação técnica dos aditivos para produtos destinados a alimentação animal.

Cardinali, R., Cullere, M., Dal Bosco, A., Mugnai, C., Ruggeri, S., Mattioli, S., Castellini, C., Marinucci, M.T & Dalle Zotte, A. (2015). Oregano, Rosemary and Vitamin E dietary supplementation in growing rabbits: effect on growth performance, carcass traits, bone development and meat chemical composition. Livestock Science, 175, 83-89.

Cardoso, V. S., Lima, C. A. R., Lima, M. E. F., Dorneles, L. E. G. & Danelli, M. G. M. (2012). Piperine as a phytogenic additive in broiler diets. Pesquisa Agropecuária Brasileira, 47, 489-496.

Cho, J. H., Kim, H. J. & Kim, I. H. (2014). Effects of phytogenic feed additive on growth performance, digestibility, blood metabolites, intestinal microbiota, meat color and relative organ weight after oral challenge with clostridium perfringens in broilers. Livestock Science, 160, 82–88.

Corino, C., Lo Fiego, D.P., Macchioni, P., Pastorelli, G., Di Giancamillo, A., Domeneghini, C. & Rossi, R. (2007). Influence of dietary conjugated linoleic acids and vitamin E on meat quality, and adipose tissue in rabbits. Meat Science, 76(1), 19–28.

Cruz, G. F. L., Xavier Neta, G. C.., Duarte, I. N. H.., Barbosa, R. V., Sousa, E. C. L., Azevedo, P. S., Costa, M. L. L., Panosso, N. M., Bessa, A. F. O., Fernandes, L. S., Borges, P. & Araújo, L.S. (2020). Carcass traits and meat quality of Lionhead rabbits. Research, Society and Development, 9(9), p. e736997887.

Dalle Zotte, A. & Szendro, Z. (2011). The role of rabbit meat as functional food. Meat Science, 88(3), 319–331.

Dalle Zotte, A., Mousavikhorshidi, H., Pizzocchero, V. & Cullere, M. (2014). Rooibos (Aspalathus linearis) is an effective functional ingredient in retarding lipid oxidation of cooked rabbit meat patties. In: Proceedings of the 60th International Congress of Meat Science and Technology. Punta del Este, Uruguay, poster 204.

Dalle Zotte, A., Celia, C. & Szendro, Z. (2016). Herbs and spices inclusion as feedstuff or additive in growing rabbit diets and as additive in rabbit meat: A review. Livestock Science, 189, 82–90.

Donhowe, E.G. & Kong, F. (2014). Beta-carotene: Digestion, Microencapsulation, and In Vitro Bioavailability. Food and Bioprocess Technology, 7(2), 338–354.

Duszynski, D.W. & Couch, L. (2013). The biology and identification of the Coccidia (apicomplexa) of rabbits of the world. 1st edition. Elsevier, Amsterdam. 352p.

Ebeid, T. A., Zeweil, H. S., Basyony, M. M., Dosoky, W. M. & Badry, H. (2013). Fortification of rabbit diets with vitamin E or selenium affects growth performance, lipid peroxidation, oxidative status and immune response in growing rabbits. Livestock Science, 155(2), 323–331.

El-Badawi, A. Y., El-Wardany, I., El-Moez, S. I. A., Helal, F. I. S., Ali, N. G. M., Shourrap, M. I. & Aboelazab, O. M. (2018). Impact of dietary Moringa oleifera leaves on intestinal pathogenic load and histological structure of growing rabbits raised under heat-stress conditions. Animal Production Science, 58(10), A-G.

El-Desoky, N. I., Hashem, N. M., Elkomy, A. & Abo-elezz, Z. R. (2017). Physiological response and semen quality of rabbit bucks supplemented with Moringa leaves ethanolic extract during summer season. Animal, 11(9), 1549–1557.

El-Hanoun, A. M., Elkomy, A. E., Fares, W. A. & Shahienl, E. H. (2014). Impact of royal jelly to improve reproductive performance of male rabbits under hot summer conditions. World Rabbit Science, 22(3), 241-248.

Ewuola, E. O., Sokunbi, O. A., Sanni, K. M., Oyedemi, O. M. & Lawal, T. T. (2015). Haematological and serum biochemical responses of rabbit does to crude Moringa oleifera leaf extract at gestation and lactation. Tropical Animal Health and Production, 47(4), 637–642.

Farag, M. R., Alagawany, M., Abd El-Hac, M. E. & Dhama, K. (2016). Nutritional and healthical aspects of Spirulina (Arthrospira) for poultry, animals and human. International Journal of Pharmacology, 12(1), 36–51.

Fernandes, R. C., Anami, J. M. & Steffens, C. A. (2019). Maçã: compostos fenólicos e saúde. Revista interdisciplinar de estudos em saúde da UNIARP, 9(2), 29-33.

Gadde, U., Kim, W. H., Oh, S. T. & Lillehoj, H. S. (2017). Alternatives to antibiotics for maximizing growth performance and feed efficiency in poultry: a review. Animal Health Research Reviews, 18, 26–45.

Gai, F., Gasco, L., Liu, H.W., Lussiana, C., Brugiapaglia, A. & Masoero, G. (2009). Effect of diet Chestnut tannin supplementation on meat quality, fatty acid profile and lipid stability in broiler rabbits. Italian Journal of Animal Science, 8, 787-789.

Gliozzi, T. M., Zaniboni, L., Maldjian, A., Luzi, F., Maertens, L. & Cerolini, S. (2009). Quality and lipid composition of spermatozoa in rabbits fed DHA and vitamin E rich diets. Theriogenology, 71, 910-919.

Gopi, M., Karthik, K., Manjunathachar, H. V., Tamilmahan, P., Kesavan, M., Dashprakash, M., Balaraju, B. L. & Purushothaman, M. R. (2014). Essential oils as a feed additive in poultry nutrition. Advance in Animal and Veterinary Sciences, 2(1), 1-7.

Hashem, N. M., Soltan, Y. A., El-Desoky, N. I., Morsy, A. S. & Sallam, S. M. A. (2019). Effects of Moringa oleifera extracts and monensin on performance of growing rabbits. Livestock Science, 228, 136-145.

Hoste, H., Torres-Acosta, J. F. J., Sandoval-Castro, C. A., Mueller-Harvey, I., Sotiraki, S., Louvandini, H., Thamsborg, S. M. & Terrill, T. H. (2015). Tannin containing legumes as a model for nutraceuticals against digestive parasites in livestock. Veterinarian Parasitology, 212(2), 5–17.

Ismail, I. E., Abdelnour, S. A., Shehata, S. A., Abd El-Hack, M. E., El-Edel, M. A., Taha, A. E., Schiavitto, M. & Tufarelli, V. (2019). Effect of Dietary Boswellia serrata Resin on Growth Performance, Blood Biochemistry, and Cecal Microbiota of Growing Rabbits. Frontiers in Veterinary Science, 6, 1-8.

Jayasena, D. D. & Jo, C. (2013). Essential oils as potential antimicrobial agents in meat and meat products: A review. Trends in Food Science & Technology, 34(2), 96-108.

Jeyakodi, S., Krishnanakumar, A. & Chellapan, D. K. (2018). Beta carotene therapeutic potential and strategies to enhance its bioavaliability. Nutrition & Food Science, 7(4), 1-6.

Karásková, K., Suchý, P. & Straková, E. (2015). Current use of phytogenic feed additives in animal nutrition: a review. Czech Journal of Animal Science, 60(12), 521–530.

Khattak, F., Ronchi, A., Castelli, P. & Sparks, N. (2014). Effects of natural blend of essential oil on growth performance, blood biochemistry, cecal morphology, and carcass quality of broiler chickens. Poultry Science, 93, 132-137.

Kirjavainen, P. V. & Gibson G. R. (1999). Healthy gut microflora and allergy: factors influencing development of the microbiota. Annals of Medicine, 31, 288-292.

Koiyama, N. T. G. (2012). Aditivos fitogênicos na produção de frangos de corte. Animal Science. Universidade Federal de Santa Catarina, Florianópolis, SC.

Kutlu, T. & Erdoðan, Z. (2010). In Poultry Feeding Phytobiotic Feed Additives. Coop Animals Congress, 07-09 October 2010, Kayseri.

Legendre, H., Saratsi, K., Voutzourakis, N., Saratsis, A., Stefanakis, A., Gombault, P., Hoste, H., Gidenne, T. & Sotiraki, S. (2018). Coccidiostatic effects of tannin-rich diets in rabbit production. PUBMED, 117(12), 3705-3713.

Lima Júnior, D. M., Monteiro, P. D. B. S., Rangel, A. H. N., Maciel, M. V., Oliveira, S. E. O. & Freire, D. A. (2010). Fatores anti-nutricionais para ruminantes. Acta Veterinaria Brasilica, 4(3), 132-143.

Liu, R.H. (2004). Potential synergy of phytochemicals in cancer prevention: mechanism of action. Journal of Nutrition, 134(12), 3479-3485.

Madhupriya, V., Shamsudeen, P., Manohar, G.R., Senthilkumar, S., Soundarapandiyan, V. & Moorthy, M. (2018). Phyto feed additives in poultry nutrition-a review. International Journal of Science, Environment and Technology, 7(3), 815-822.

Maenner, K., Vahjen, W. & Simon, O. (2011). Studies on the effects of essential-oil based feed additives on performance, ileal nutrient digestibility, and selected bacterial groups in the gastrointestinal tract of piglets. Journal of Animal Science, 89, 2106-2112.

Maghsoudlou, A., Mahoonak, A.S., Mohebodini, H. & Toldra, F. (2019). Royal jelly: Chemistry, storage and bioactivities. Journal of Apicultural Science, 63(1), 17– 40.

Mangiagalli M. G., Cesari, V., Cerolini, S., Luzi, F. & Toschi, I. (2012). Effect of lycopene supplementation on semen quality and reproductive performance in rabbit. World Rabbit Science, 20, 141–148.

Martins, N., Roriz, C. L., Morales, P., Barros, L. & Ferreira, I. C. F. R. (2016). Food colorants: Challenges, opportunities and current desires of agro-industries to ensure consumer expectations and regulatory practices. Trends in Food Science & Technology, 52, 1–15.

Menten, J. F. M. (2002). Probióticos, Prebióticos e Aditivos Fitogênicos na nutrição de aves. In: II Simpósio Sobre Nutrientes Na Alimentação Animal. Anais... Uberlândia, p. 251-276.

Min, B. R., Attwood, G. T., McNabb, W. C., Molan, A. L. & Barry, T. N. (2005). The effect of condensed tannins from Lotus corniculatus on the proteolytic activities and growth of rumen bacteria. Animal Feed Science and Technology, 121(1), 45-58.

Moritz, B. & Tramonte, V. L. C. (2006). Biodisponibilidade do licopeno. Revista de Nutrição, 19(2), 265-273.

Mueller, K., Blum, N.M., Kluge, H. & Mueller, A.S. (2012). Influence of broccoli extract and various essential oils on performance and expression of xenobiotic-and antioxidant enzymes in broiler chickens. British Journal of Nutrition, 108(4), 588-602.

Naumann, H. D., Tedeschi, L. O., Zeller, W. E. & Huntley, N. F. (2017). The role of condensed tannins in ruminant animal production: advances, limitations and future directions. Revista Brasileira de Zootecnia, 46(12), 929-949.

Nijveldt, R. J., Van Nood, E., Van Hoorn, D. E. C., Boelens, P. G., Van Norren, K. & Van Leeuwen, P. A. M. (2001). Flavonoids: a review of probable mechanisms of action and potential applications. The American journal of clinical nutrition, 74, 418-425.

Osman, A., Abd-Elaziz, S., Salama, A., Eita, A. A. & Sitohy, M. (2019). Health protective actions of phycocyanin obtained from an Egyptian isolate of Spirulina platensis on albino rats. EurAsia Journal of BioScience, 13, 105–112.

Pakandl, M. (2009). Coccidia of rabbit: a review. Parasitology, 56(3), 153–166.

Palozza, P., Parrone, N., Simone, R.E. & Catalano, A. (2010). Lycopene in atherosclerosis prevention: an integrated scheme of the potential mechanisms of action from cell culture studies. PUBMED, 504(1), 26–33.

Pereira, R. J. & Cardoso, M. D. G. (2012). Vegetable secondary metabolites and antioxidants benefits. Journal of Biotechnology and Biodiversity, 3, 146-152.

Peterson, J. D. (1998). Flavonoids: Dietary occurrence and biochemical activity. Nutrition Research, 18, 1995–2018.

Potenza, M. R. (2004). Produtos naturais para o controle de pragas. In: X Reunião Itinerante de Fitossanidade do Instituto Biológico: Café, 5., 2004, Mooca. Anais... São Paulo,SP, 2004. p.89-100.

Rodriguez-Amaya, D. B. (2016). Natural food pigments and colorants. Current Opinion in Food Science, 7, 20-26.

Santos, P. L., Prando, M. B., Morando, R., Pereira, G. V. N. & Kronka, A. Z. (2013). Utilização de extratos vegetais em proteção de plantas. Centro Científico Conhecer - Goiânia, 9(17), 2562-2576.

Shahidi, F. & Ambigaipalan, P. (2015). Phenolics and polyphenolics in foods, beverages and spices: Antioxidant activity and health effects – A review. Journal of Functional Foods, 18, 820–897.

Singh, B. N., Singh, B. R., Singh, R. L., Prakash, D., Dhakarey, R., Upadhyay, G. & Singh,H. B. (2009). Oxidative DNA damage protective activity, antioxidant and anti-quorum sensing potentials of Moringa oleifera. Food and Chemical Toxicology, 47(6), 1109-1116.

Souza, C. G., Moura, A. K. B., Silva, J. N. P., Soares, K. O., Silva, J. V. C. & Vasconcelos, P. C. (2019). Fatores anti-nutricionais de importância na nutrição animal: Composição e função dos compostos secundários. PUBVET, 13(5), 1-19.

Talipov, S. A., Mamadrakhimov, A. A., Tiljakov, Z. G., Dowd, M. K., Ibragimov, B. T. & Xonkeldieva, M. T. (2009). The Crystal and Molecular Structure of Dianhydrogossypol. Journal of the American Oil Chemistry Society, 86(3), 207-213.

Tozer, K. N. (2001). Qualin improvement and shelf-life extension of fish filets from three aquaculture species. Dissertação (Mestrado) – University of Guelph. Ontário, Canadá.

Valero, M. V., Prado, R. M., Zawadski, F., Eiras, C. E., Madrona, G. S. & Prado, I. N. (2014). Propolis and essential oils additives in the diets improved animal performance and feed efficiency of bulls finished in feedlot. Acta Scientiarum Animal Sciences, 32, 419-426.

Volp, A. C. P., Renhe, I. R. T. & Stringueta, P. C. (2011). Carotenoides: pigmentos naturais como compostos bioativos. Revista Brasileira de Nutrição Clinica, 26(4), 291-298.

Wahlin, S., Floderus, Y., Stål, P. & Harper, P. (2011). Erythropoietic protoporphyria in Sweden: demographic, clinical, biochemical and genetic characteristics. Journal of Internal Medicine, 269(3), 278-288.

Xie, Y., Li, W., Zhu, L., Zhai, S., Qin, S. & Du, Z. (2019). Effects of phycocyanin in modulating the intestinal microbiota of mice. Microbiology, 8(9), 1-11.

Yang, C., Chowdhury, M.A., Huo, Y. & Gong, J. (2015). Phytogenic Compounds as Alternatives to In-Feed Antibiotics: Potentials and Challenges in Application. Pathogens, 4(1), 137–156.

Yitbarek, M. B. (2015). Phytogenics as feed additives in poultry production: a review. International Journal of Extensive Research, 3, 49–60.

Zhang, G. G., Yang, Z. B., Wang, Y. & Yang, W. R. (2013). Effects of Astragalus membranaceus root processed to different particle sizes on growth performance, antioxidant status, and serum metabolites of broiler chickens. Poultry Science, 92, 178-183.

Zhang, W., Xiao, S., Samaraweera, H., Lee, E. J. & Ahn, D. U. (2010). Improving functional value of meat products. Meat Science, 86, 15-31.



How to Cite

MIRANDA, V. M. de M. C. .; SILVA, L. A. L. da .; LEITE, S. M. .; RIBEIRO, B. L. .; BATISTA , P. R. .; SILVA, E. M. T. T. da .; CASTILHA, L. D. . Phytogenic compounds and bioactive substances for rabbits: benefits on reproduction, well-being and meat quality. Research, Society and Development, [S. l.], v. 10, n. 12, p. e306101220103, 2021. DOI: 10.33448/rsd-v10i12.20103. Disponível em: Acesso em: 24 feb. 2024.



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