Intramammary application of curcumin in ewes with subclinical mastitis and healthy ewes: effect on milk production and quality
DOI:
https://doi.org/10.33448/rsd-v10i15.20262Keywords:
Mastitis; Herbal Extract; Sheep.Abstract
The objective of this work was to evaluate the effect of intramammary application of curcumin in ewes with subclinical mastitis and healthy ewes on milk production, milk quality and ewes health. For this, two experiments were carried out: Experiment I: 10 ewes with chronic subclinical mastitis were divided into two groups of five ewes each: a) control, 3 mL of mineral oil was applied intra-mammary; b) curcumin, applied 2 mg of curcumin diluted in mineral oil, intra-mammary. There was a treatment effect, that is, ewes that received the application of curcumin had higher milk production. There was a treatment effect for the percentage of protein in milk, as well as a trend of treatment effect for lactose and total solders; with emphasis on lower levels of protein, lactose and non-fat solids in the ewes of the curcumin group compared to the control. We found an interaction between treatment versus day for milk protein on days 7 and 14 after application; lower in the curcumin group. Day effect was verified for erythrocyte and leukocyte counts, as well as hemoglobin concentrations in both groups. For experiment II we used 80 ewes divided into 4 groups: control, T2, T3 and T4 (2, 3 and 4 mg of curcumin diluted in mineral oil via intra-mammary, respectively). Day effect was observed for the T2 group, that is, there was an increase in milk production from day 1 to 20. Somatic cell count (SCC) was effected by treatment, day and interaction between treatment versus day. The highest SCC was observed in the T4 group. Of the group of ewes, 17 had problems in the mammary gland after applying the four treatments, and five ewes developed severe environmental mastitis and died; and the other animals also tested positive for subclinical mastitis in the racket test (California Mastitis Test). In general, the use of curcumin intra-mammary at the doses tested has no curative effect, as well as when used in healthy sheep, it has a negative effect, affecting the quality of the milk (increased CCS). Furthermore, the application of intra-mammary products (mineral oil or oil + curcumin) is a high risk for the animal; therefore, we do not recommend.
References
Bencini, R. & Pulina, G. (1997). The quality of sheep milk: a Review. Wool Technology and Sheep Breeding, 45(3), 182-220.
Caboni, P., Manis, C. & Ibba I. et al. (2017). Compositional profile of ovine milk with a high somatic cell count: A metabolomics approach. Int. Dairy J. 69, 33-39.
Deolindo, G. L., Molosse, V. L., & Dilda, A., et al. (2021). Lacaune ewes with subclinical mastitis: effects of intramammary application of própolis. Research, Society and Development, 10(2).
Feldman, B. F., Zinkl, J. G. & Jain, C. N. (2000). Schalm’s Veterinary Hematology. 5th edn. San Diego: Academic Press, 932, 2000.
Galli, G. M., Da Silva, A. S. & Biazus, A. H. et al. (2018) Feed addition of curcumin to laying hens showed anticoccidial effect, and improved egg quality and animal health. Research in veterinary science, 118, 101-106.
Hassan, H. A. (1995). Effects of crossing and environmental factors on production and some constituents of milk in Ossimi and Saidi sheep and their crosses with Chios. Small Ruminant Research, 18,165-172.
Jaguezeski, A. M., Perin, G. & Bottari, N. B. et al. (2018) Addition of curcumin to the diet of dairy sheep improves health, performance and milk quality. Animal Feed Science and Technology, 246, 144-157.
Jaguezeski, A. M., Perin, G., & Crecencio, R. B. (2018). et al. Addition of curcumin in dairy sheep diet in the control of subclinical mastitis. Acta Scientiae Veterinariae, 46(7).
Larsgard, A. G. & Vaabenoe, A. Genetic and environmental causes of variation in mastitis in sheep. Small ruminant research, 12(3), 339-347, 1993.
Leitner, G., Chaffer, M. & Shamay, A. et al. (2004). Changes in milk composition as affected by subclinical mastitis in sheep. Journal of dairy science, 87(1), 46-52.
Li, T., Zhao, Y., & Matthews, K. et al. (2021). Antibacterial activity against Staphylococcus aureus of curcumin-loaded chitosan spray coupled with photodynamic treatment. LWT, 134, 110073.
Marti, A. D. O., Diaz, J. R. & Molina, M. P. et al. (2013). Quantification of milk yield and composition changes as affected by subclinical mastitis during the current lactation in sheep. Journal Dairy Science. 96, 7698-7708.
Markey, B., Leonard F. & Archambault M. et al. (2013). Clinical Veterinary Microbiology, segunda ed., Mosby Elsevier, London.
Molosse, V.; Souza, C. F. & Baldissera, M. D. et al. (2019). Diet supplemented with curcumin for nursing lambs improves animal growth, energetic metabolism, and performance of the antioxidant and immune systems. Small Ruminant Research. 170, 74-81.
Noorafshan, A. & Ashkani-Esfahani, S. (2013). A Review of Therapeutic Effects of Curcumin. Current Pharmaceutical Design. 19(11), 2032–2046.
National Mastitis Council (NMC). (2004). Microbiological procedures for the diagnosis of bovine udder infection and determination of milk quality. quarta ed., NMC, Verona.
Park, Y. W., Juarez, M. & Ramos, M. et al. (2007). Physico-chemical characteristics of goat and sheep milk. Small Ruminant Research. 68, 88–113.
Pereira, A. S., Shitsuka, D. M. & Pereira, F. J. et al. (2018). Methodology of cientific research. [e-Book]. Santa Maria City. UAB / NTE / UFSM Editors. May, 14th, 2020.Available at: https://repositorio.ufsm.br/bitstream/handle/1/15824/Lic_Computacao_Metodologia-Pesquisa-Cientifica.pdf?sequence=1.
Rohenkohl, J. E., Correa, G. F. & De Azambuja, D. F. et al. (2011). O agronegócio de leite de ovinos e caprinos. Indicadores Econômicos FEE. 39 (2).
Verissimo, C. J., Zafalon, L. F. & Otsuk, I. P. et al. (2021). Prejuízos causados pela mastite em ovelhas Santa Inês. Arquivos do Instituto Biológico. 77, 583-591.
Yun, D. G. & Lee, D. G. (2016). Antibacterial activity of curcumin via apoptosis-like response in Escherichia coli. Applied microbiology and biotechnology. 100(12), 5505-5514.
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Copyright (c) 2021 Bruno Giorgio de Oliveira Cécere; Guilherme Luiz Deolindo; Vitor Luiz Molosse; Lilian Kolling Girardini ; Marcelo Vedovatto; Aleksandro Schafer da Silva
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