Development of a functional ingredient rich in hesperidin from citrus fruit wastes
DOI:
https://doi.org/10.33448/rsd-v10i12.20530Keywords:
Functional food; Phytochemicals; Food waste; HPLC.Abstract
The peels of citrus fruits contain a high concentration of bioactive compounds. Among these compounds, hesperidin stands out for its beneficial health effects. This study had the objective of evaluating the hesperidin content in peel samples of different citrus fruit and to propose the development of a functional product obtained from these peels. The peels were lyophilized and had the total flavonoids analyzed by high-performance liquid chromatography. The peels of several fruits were dried in a microwave oven and ground in a blender to obtain a homogeneous powder. This material was submitted to extraction and quantification of hesperidin. The highest concentrations were found in the ‘Ponkan’ tangerine, ‘Murcott’ tangerine and ‘Navel’ orange, while the lowest were found in the ‘Sicilian’ lemon and ‘Lima’ orange. The functional ingredient obtained from the ground peels after microwave drying can be used for direct consumption or to enrich food preparations.
References
Abrahão, S. A., Pereira, R. G. F. A., Duarte, S. M. S., Lima, A. R., Alvarenga, D. J., & Ferreira, E. B. (2010). Compostos bioativos e atividade antioxidante do café (Coffea arabica L.). Ciência e Agrotecnologia, 34(2). https://doi.org/10.1590/s1413-70542010000200020
Anagnostopoulou, M. A., Kefalas, P., Papageorgiou, V. P., Assimopoulou, A. N., & Boskou, D. (2006). Radical scavenging activity of various extracts and fractions of sweet orange peel (Citrus sinensis). Food Chemistry, 94(1). https://doi.org/10.1016/j.foodchem.2004.09.047
Arabbi, P. R., Genovese, M. I., & Lajolo, F. M. (2004). Flavonoids in Vegetable Foods Commonly Consumed in Brazil and Estimated Ingestion by the Brazilian Population. Journal of Agricultural and Food Chemistry, 52(5). https://doi.org/10.1021/jf0499525
Assefa, A. D., Saini, R. K., & Keum, Y. S. (2017). Fatty acids, tocopherols, phenolic and antioxidant properties of six citrus fruit species: a comparative study. Journal of Food Measurement and Characterization, 11(4). https://doi.org/10.1007/s11694-017-9546-x
Bae, J. M., Lee, E. J., & Guyatt, G. (2008). Citrus fruit intake and stomach cancer risk: A quantitative systematic review. Gastric Cancer, 11(1). https://doi.org/10.1007/s10120-007-0447-2
Cazarolli, L., Zanatta, L., Alberton, E., Bonorino Figueiredo, M. S., Folador, P., Damazio, R., & Barreto Silva, F. R. (2008). Flavonoids: Prospective Drug Candidates. Mini-Reviews in Medicinal Chemistry, 8(13). https://doi.org/10.2174/138955708786369564
Cicero, N., Corsaro, C., Salvo, A., Vasi, S., Giofré, S. V., Ferrantelli, V., & Dugo, G. (2015). The metabolic profile of lemon juice by proton HR-MAS NMR: The case of the PGI Interdonato Lemon of Messina. Natural Product Research, 29(20), 1894–1902. https://doi.org/10.1080/14786419.2015.1012166
Coutinho, M. A. S., Muzitano, M. F., & Costa, S. S. (2009). Flavonoids: Potential therapeutic agents for the inflammatory process. Revista Virtual de Química, 1(3). https://doi.org/10.5935/1984-6835.20090024
Craveiro, A., Craveiro, A., Pinto, Í., & Mendes, F. (2009). Processo de obtenção de bioflavonóides cítricos, notadamente hesperidina, a partir de polpa cítrica peletizada e resíduos de frutas cítricas. https://www.escavador.com/patentes/351341/processo-de-obtencao-de-bioflavonoides-citricos-notadamente-hesperidina-a
Devi, K. P., Rajavel, T., Nabavi, S. F., Setzer, W. N., Ahmadi, A., Mansouri, K., & Nabavi, S. M. (2015). Hesperidin: A promising anticancer agent from nature. Industrial Crops and Products, 76, 582–589. https://doi.org/10.1016/j.indcrop.2015.07.051
Fernández-Bedmar, Z., Anter, J., Alonso-Moraga, A., Martín de las Mulas, J., Millán-Ruiz, Y., & Guil-Luna, S. (2017). Demethylating and anti-hepatocarcinogenic potential of hesperidin, a natural polyphenol of Citrus juices. Molecular Carcinogenesis, 56(6). https://doi.org/10.1002/mc.22621
Garg, A., Garg, S., Zaneveld, L. J. D., & Singla, A. K. (2001). Chemistry and pharmacology of the Citrus bioflavonoid hesperidin. Phytotherapy Research. https://doi.org/10.1002/ptr.1074
Gillman, M. W., Cupples, L. A., Posner, B. M., Gagnon, D., Ellison, R. C., Castelli, W. P., & Wolf, P. A. (1995). Protective Effect of Fruits and Vegetables on Development of Stroke in Men. JAMA: The Journal of the American Medical Association, 273(14), 1113–1117. https://doi.org/10.1001/jama.1995.03520380049034
Gondim, J. A. M., Moura, M. de F. V., Dantas, A. S., Medeiros, R. L. S., & Santos, K. M. (2005). Composição centesimal e de minerais em cascas de frutas. Ciência e Tecnologia de Alimentos, 25(4). https://doi.org/10.1590/s0101-20612005000400032
He, D., Shan, Y., Wu, Y., Liu, G., Chen, B., & Yao, S. (2011). Simultaneous determination of flavanones, hydroxycinnamic acids and alkaloids in citrus fruits by HPLC-DAD-ESI/MS. Food Chemistry, 127(2). https://doi.org/10.1016/j.foodchem.2010.12.109
He, F. J., Nowson, C. A., Lucas, M., & MacGregor, G. A. (2007). Increased consumption of fruit and vegetables is related to a reduced risk of coronary heart disease: Meta-analysis of cohort studies. Journal of Human Hypertension, 21(9), 717–728. https://doi.org/10.1038/sj.jhh.1002212
Homayouni, F., Haidari, F., Hedayati, M., Zakerkish, M., & Ahmadi, K. (2018). Blood pressure lowering and anti-inflammatory effects of hesperidin in type 2 diabetes; a randomized double-blind controlled clinical trial. Phytotherapy Research, 32(6). https://doi.org/10.1002/ptr.6046
Matias, I., Diniz, L. P., Buosi, A., Neves, G., Stipursky, J., & Gomes, F. C. A. (2017). Flavonoid hesperidin induces synapse formation and improves memory performance through the astrocytic TGF-β1. Frontiers in Aging Neuroscience, 9(JUN). https://doi.org/10.3389/fnagi.2017.00184
Mencherini, T., Campone, L., Piccinelli, A. L., García Mesa, M., Sánchez, D. M., Aquino, R. P., & Rastrelli, L. (2013). HPLC-PDA-MS and NMR characterization of a hydroalcoholic extract of citrus aurantium L. Var. amara Peel with antiedematogenic activity. Journal of Agricultural and Food Chemistry, 61(8). https://doi.org/10.1021/jf302815t
Metro, D., Tardugno, R., Papa, M., Bisignano, C., Manasseri, L., Calabrese, G., & Cicero, N. (2018). Adherence to the Mediterranean diet in a Sicilian student population. Natural Product Research, 32(15). https://doi.org/10.1080/14786419.2017.1402317
Nielsen, I. L. F., Chee, W. S. S., Poulsen, L., Offord-Cavin, E., Rasmussen, S. E., Frederiksen, H., & Williamson, G. (2006). Bioavailability is improved by enzymatic modification of the citrus flavonoid hesperidin in humans: A randomized, double-blind, crossover trial. Journal of Nutrition, 136(2), 404–408. https://doi.org/10.1093/jn/136.2.404
Ohara, T., Muroyama, K., Yamamoto, Y., & Murosaki, S. (2016). Oral intake of a combination of glucosyl hesperidin and caffeine elicits an anti-obesity effect in healthy, moderately obese subjects: A randomized double-blind placebo-controlled trial. Nutrition Journal, 15(1). https://doi.org/10.1186/s12937-016-0123-7
Oliveira, L. M. N. (2014). Quantificação de rutina, atividades antioxidante e antimicrobiana de extratos de polpas e subprodutos de frutas tropicais. Universidade Federal do Ceará. Retrieved from https://ppgcta.ufc.br/wp-content/uploads/2018/08/leilanne-marcia-nogueira-oliveira.pdf
Parhiz, H., Roohbakhsh, A., Soltani, F., Rezaee, R., & Iranshahi, M. (2015). Antioxidant and anti-inflammatory properties of the citrus flavonoids hesperidin and hesperetin: An updated review of their molecular mechanisms and experimental models. Phytotherapy Research. https://doi.org/10.1002/ptr.5256
Pereira, R. M. S., López, B. G. C., Diniz, S. N., Antunes, A. A., Moreno Garcia, D., Rocha Oliveira, C., & Marcucci, M. C. (2017). Quantification of Flavonoids in Brazilian Orange Peels and Industrial Orange Juice Processing Wastes. Agricultural Sciences, 08(07). https://doi.org/10.4236/as.2017.87048
Pio, R. M., Figueiredo, J. O., Stuchi, E. S., & Cardoso, S. A. B. (2005). Variedades copas. In Mattos Junior, D., Negri, J. D., Pio, R. M., & Pompeu Junior, J (Eds.), Citros (pp. 37-60). Cordeirópolis: Centro APTA Citros Sylvio Moreira.
Salden, B. N., Troost, F. J., De Groot, E., Stevens, Y. R., Garcés-Rimón, M., Possemiers, S., & Masclee, A. A. (2016). Randomized clinical trial on the efficacy of hesperidin 2S on validated cardiovascular biomarkers in healthy overweight individuals1,2. American Journal of Clinical Nutrition, 104(6). https://doi.org/10.3945/ajcn.116.136960
Salvo, A., Bruno, M., La Torre, G. L., Vadalà, R., Mottese, A. F., Saija, E., & Dugo, G. (2016). Interdonato lemon from Nizza di Sicilia (Italy): chemical composition of hexane extract of lemon peel and histochemical investigation. Natural Product Research, 30(13). https://doi.org/10.1080/14786419.2015.1115999
Stoll, L., Flôres, S. H., & Silveira Thys, R. C. (2015). Citrus peel fiber and its application as a fat substitute in loaf brad. Citrus Peel Fiber and Its Application as a Fat Substitute in Loaf Brad, 45(3). https://doi.org/10.1590/0103-8478cr20131503
Storrer, D.; Meireles, K. H.; Perly, M. D.; Lima, R. da S.; & Mattanna, P. (2017). Desenvolvimento de pão com adição de farinha de bagaço de laranja: análises sensoriais, físico-químicas e microbiológicas. Biociências, Biotecnologia e Saúde, 9(18), 43–50.
Wang, Y. C., Chuang, Y. C., & Hsu, H. W. (2008). The flavonoid, carotenoid and pectin content in peels of citrus cultivated in Taiwan. Food Chemistry, 106(1). https://doi.org/10.1016/j.foodchem.2007.05.086
Xu, M., Tian, G., Zhao, C., Ahmad, A., Zhang, H., Bi, J., & Zheng, J. (2017). Infrared Drying as a Quick Preparation Method for Dried Tangerine Peel. International Journal of Analytical Chemistry, 2017. https://doi.org/10.1155/2017/6254
Zhou, Y., Zheng, J., Li, Y., Xu, D. P., Li, S., Chen, Y. M., & Li, H. Bin. (2016). Natural polyphenols for prevention and treatment of cancer. Nutrients. https://doi.org/10.3390/nu8080515
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Copyright (c) 2021 Camila Tomé da Cunha; Andressa Fontes Oliveira; Victor Borges Fernandes; Francisca Noélia Pereira Mendes; Ícaro Gusmão Pinto Vieira
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