Animal models used to study the temporomandibular joint: literature review




Models, Animal; Temporomandibular Joint; Temporomandibular joint disorders.


The temporomadibular joint (TMJ) is considered one of the most complex joints in the human body. It is involved in multipl functions, including chewing and phonation. It is considered na extremely adaptative structure. It is involved in multiple functions, including several pathologies can affect the TMJ and result in dysfunctions that significantly interfere in patients' lives. Temporomandibular disorders (TMD) are associated with a large number of etiologies. Animal experimental models represent a possibility for the anatomical, histological and physiological study of this structure, the induction of dysfunctions and establishment of treatments. The aim of this literature review is to present and discuss the use of animal models for the study of TMJ. The literature review was carried out through a literature search in the Pubmed (, Scielo ( and Bireme ( databases. According to the literature review, so far, no animal model is totally similar to the human TMJ. This characteristic represents a limiting factor in the investigation of possible surgical and non-surgical therapies for TMDs. Since there is no single model, researchers must choose the animal model that most applies to the objective of the study to be carried out.


Abdrabuh, A., Baljon, K., & Alyami, Y. (2020). Impact of estrogen therapy on temporomandibular joints of rats: Histological and hormone analytical study, The Saudi Dental Journal. In Press, Corrected Proof.

Abramowicz, S., Crotts, S. J., Hollister, S. J., & Goudy, S. (2021). Tissue-engineered vascularized patient-specific temporomandibular joint reconstruction in a Yucatan pig model. Oral Surgery, Oral Medicine, Oral Pathology And Oral Radiology, 132(2), 145-152.

Ali, A. M., & Sharawy, M. M. (1994). Histopathological changes in rabbit craniomandibular joint associated with experimentally induced anterior disk displacement (ADD). Journal of Oral Pathology & Medicine, 23(8), 364-374.

Almarza, A. J., Brown, B. N., Arzi, B., Ângelo, D. F., Chung, W., Badylak, S. F., & Detamore, M. (2018). Preclinical Animal Models for Temporomandibular Joint Tissue Engineering. Tissue engineering. Part B, Reviews, 24(3), 171–178.

Ângelo, D. F., Monje, F. G., González-García, R., Little, C. B., Mónico, L., Pinho, M., & Santos, F. A. et al. (2017). Bioengineered temporomandibular joint disk implants: study protocol for a two-phase exploratory randomized preclinical pilot trial in 18 black merino sheep (TEMPOJIMS). JMIR Research Protocols, 6(3), e37.

Angelo, D. F., Morouço, P., Alves, N., Viana, T., Santos, F., González, R., Monje, F., Macias, D., Carrapiço, B., Sousa, R., Cavaco-Gonçalves, S., Salvado, F., Peleteiro, C., & Pinho, M. (2016). Choosing sheep (Ovis aries) as animal model for temporomandibular joint research: Morphological, histological and biomechanical characterization of the joint disc. Morphologie: bulletin de l'Association des anatomistes, 100(331), 223–233.

Artuzi, F. E., Langie, R., Abreu, M. C., Quevedo, A. S., Corsetti, A., Ponzoni, D., & Puricelli, E. (2016). Rabbit model for osteoarthrosis of the temporomandibular joint as a basis for assessment of outcomes after intervention. The British Journal of Oral & Maxillofacial Surgery, 54(5), e33-e37.

Artuzi, F. E., Puricelli, E., Baraldi, C. E., Quevedo, A. S., & Ponzoni, D. (2020). Reduction of osteoarthritis severity in the temporomandibular joint of rabbits treated with chondroitin sulfate and glucosamine. PloS one, 15(4), e0231734.

Axelsson, S., Holmlund, A., & Hjerpe, A. (1992). An experimental model of osteoarthrosis in the temporomandibular joint of the rabbit. Acta Odontologica Scandinavica, 50(5), 273-280.

Basting, R. T., Napimoga, M. H., de Lima, J. M., de Freitas, N. S., & Clemente-Napimoga, J. T. (2021). Fast and accurate protocol for histology and immunohistochemistry reactions in temporomandibular joint of rats. Archives of Oral Biology, 126, 105115.

Berg R. (1973). Contribution to the applied and topographical anatomy of the temporomandibular joint of some domestic mammals with particular reference to the partial resp. total resection of the articular disc. Folia Morphologica, 21(2), 202-204.

Bermejo, A., González, O., & González, J. M. (1993). The pig as an animal model for experimentation on the temporomandibular articular complex. Oral Surgery, Oral Medicine, and Oral Pathology, 75(1), 18-23.

Ciochon, R. L., Nisbett, R. A., & Corruccini, R. S. (1997). Dietary consistency and craniofacial development related to masticatory function in minipigs. Journal of Craniofacial Genetics and Developmental Biology, 17(2), 96-102.

Cornish, R. J., Wilson, D. F., Logan, R. M., & Wiebkin, O. W. (2006). Trabecular structure of the condyle of the jaw joint in young and mature sheep: a comparative histomorphometric reference. Archives of Oral Biology, 51(1), 29-36.

Detamore, M. S., Athanasiou, K. A., & Mao, J. (2007). A call to action for bioengineers and dental professionals: directives for the future of TMJ bioengineering. Annals of Biomedical Engineering, 35(8), 1301-1311.

El-Hakim, I. E., Abdel-Hamid, I. S., & Bader, A. (2005). Tempromandibular joint (TMJ) response to intra-articular dexamethasone injection following mechanical arthropathy: a histological study in rats. Int J Oral Maxillofac Surg, 34(3), 305-10.

Embree, M. C., Iwaoka, G. M., Kong, D., Martin, B. N., Patel, R. K., Lee, A. H., & Nathan, J. M. et al. (2015). Soft tissue ossification and condylar cartilage degeneration following TMJ disc perforation in a rabbit pilot study. Osteoarthritis and Cartilage, 23(4), 629-639.

Fujita, S., & Hoshino, K. (1989). Histochemical and immunohistochemical studies on the articular disk of the temporomandibular joint in rats. Acta Anat, 134(1), 26-30.

Ghassemi Nejad, S., Kobezda, T., Tar, I., & Szekanecz, Z. (2017). Development of temporomandibular joint arthritis: The use of animal models. Joint bone spine, 84(2), 145–151.

Gulses, A., Bayar, G. R., Aydintug, Y. S., Sencimen, M., Erdogan, E., & Agaoglu, R. (2013). Histological evaluation of the changes in temporomandibular joint capsule and retrodiscal ligaments following autologous blood injection. Journal of Cranio-Maxillo-Facial Surgery, 41(4), 316-320.

Hakim, M. A., Guastaldi, F., Liapaki, A., Ahn, D. Y., Mueller, M. L., Troulis, M. J., & McCain, J. P. (2020). In vivo investigation of temporomandibular joint regeneration: development of a mouse model. International journal of oral and maxillofacial surgery, 49(7), 940–944.

Herring, S. W., Decker, J. D., Liu, Z. J., & Ma, T. (2002). Temporomandibular joint in miniature pigs: anatomy, cell replication, and relation to loading. The Anatomical Record, 266(3), 152-166.

Hu, Y., Yang, H. F., Li, S., Chen, J. Z., Luo, Y. W., & Yang, C. (2012). Condyle and mandibular bone change after unilateral condylar neck fracture in growing rats. International Journal of Oral and Maxillofacial Surgery, 41(8), 912-921.

Huang, L., Zhang, L., Li, H., Yan, J., Xu, X., & Cai, X. (2020). Growth pattern and physiological characteristics of the temporomandibular joint studied by histological analysis and static mechanical pressure loading testing. Archives of Oral Biology, 111, 104639.

Kalpakci, K. N., Willard, V. P., Wong, M. E., & Athanasiou, K. A. (2011). An interspecies comparison of the temporomandibular joint disc. Journal of Dental Research, 90(2), 193-198.

King, A. M., Cranfield, F., Hall, J., Hammond, G., & Sullivan, M. (2010). Radiographic anatomy of the rabbit skull with particular reference to the tympanic bulla and temporomandibular joint: Part 1: Lateral and long axis rotational angles. Veterinary Journal, 186(2), 232-243.

Kol, A., Arzi, B., Athanasiou, K. A., Farmer, D. L., Nolta, J. A., Rebhun, R. B., & Chen, X. et al. (2015). Companion animals: Translational scientist's new best friends. Science Translational Medicine, 7(308), 308-21.

Kuyinu, E. L., Narayanan, G., Nair, L. S., & Laurencin, C. T. (2016). Animal models of osteoarthritis: classification, update, and measurement of outcomes. Journal of orthopaedic surgery and research, 11, 19.

Kyllar, M., Paral, V., Pyszko, M., & Doskarova, B. (2017). Facial pillars in dogs: an anatomical study. Anatomical Science International, 92(3), 343-351.

Kyllar, M., Putnová, B., Jekl, V., Stehlík, L., Buchtová, M., & Štembírek, J. (2018). Diagnostic imaging modalities and surgical anatomy of the temporomandibular joint in rabbits. Laboratory Animals, 52(1), 38-50.

Lai, W. F., Tsai, Y. H., Su, S. J., Su, C. Y., Stockstill, J. W., & Burch, J. G. (2005). Histological analysis of regeneration of temporomandibular joint discs in rabbits by using a reconstituted collagen template. International Journal of Oral and Maxillofacial Surgery, 34(3), 311-320.

Ma, B., Sampson, W., Fazzalari, N., Wilson, D., & Wiebkin, O. (2002). Experimental forward mandibular displacement in sheep. Archives of Oral Biology, 47(1), 75-84.

Mazuqueli Pereira, E., Basting, R. T., Abdalla, H. B., Garcez, A. S., Napimoga, M. H., & Clemente-Napimoga, J. T. (2021). Photobiomodulation inhibits inflammation in the temporomandibular joint of rats. Journal of photochemistry and photobiology. B, Biology, 222, 112281.

Mills, D. K., Daniel, J. C., & Scapino, R. (1988). Histological features and in-vitro proteoglycan synthesis in the rabbit craniomandibular joint disc. Archives of Oral Biology, 33(3), 195-202.

Mizoguchi, I., Takahashi, I., Nakamura, M., Sasano, Y., Sato, S., Kagayama, M., & Mitani, H. (1996). An immunohistochemical study of regional differences in the distribution of type I and type II collagens in rat mandibular condylar cartilage. Archives of Oral Biology, 41(8-9), 863-869.

Monteiro, J., Guastaldi, F., Troulis, M. J., McCain, J. P., & Vasconcelos, B. (2021). Induction, Treatment, and Prevention of Temporomandibular Joint Ankylosis-A Systematic Review of Comparative Animal Studies. Journal of oral and maxillofacial surgery : official journal of the American Association of Oral and Maxillofacial Surgeons, 79(1), 109–132.e6.

Nicot, R., Barry, F., Chijcheapaza-Flores, H., Garcia-Fernandez, M. J., Raoul, G., Blanchemain, N., & Chai, F. (2021). A Systematic Review of Rat Models With Temporomandibular Osteoarthritis Suitable for the Study of Emerging Prolonged Intra-Articular Drug Delivery Systems. Journal of oral and maxillofacial surgery: official journal of the American Association of Oral and Maxillofacial Surgeons, 79(8), 1650–1671.

Porto, G. G., Vasconcelos, B. C., Andrade, E. S., & Silva-Junior, V. A. (2010). Comparison between human and rat TMJ: anatomic and histopathologic features. Acta Cir Bras, 25(3), 290-293.

Puricelli, E., Ponzoni, D., Munaretto, J. C., Corsetti, A., & Leite, M. G. (2012). Histomorphometric analysis of the temporal bone after change of direction of force vector of mandible: an experimental study in rabbits. Journal of Applied Oral Science, 20(5), 526-530. doi: 10.1590/s1678-77572012000500006

Puricelli, E., Artuzi, F. E., Ponzoni, D., & Quevedo, A. S. (2019). Condylotomy to Reverse Temporomandibular Joint Osteoarthritis in Rabbits. Journal of oral and maxillofacial surgery: official journal of the American Association of Oral and Maxillofacial Surgeons, 77(11), 2230–2244.

Sato, M., Tsutsui, T., Moroi, A., Yoshizawa, K., Aikawa, Y., Sakamoto, H., & Ueki, K. (2019). Adaptive change in temporomandibular joint tissue and mandibular morphology following surgically induced anterior disc displacement by bFGF injection in a rabbit model. Journal of Cranio-Maxillo-Facial Surgery, 47(2), 320-327.

Štembírek, J., Kyllar, M., Putnová, I., Stehlík, L., & Buchtová, M. (2012). The pig as an experimental model for clinical craniofacial research. Laboratory Animals, 46(4), 269-279.

Sun, Z., Liu, Z. J., & Herring, S. W. (2002). Movement of temporomandibular joint tissues during mastication and passive manipulation in miniature pigs. Archives of oral biology, 47(4), 293-305.

Vapniarsky, N., Aryaei, A., Arzi, B., Hatcher, D. C., Hu, J. C., & Athanasiou, K. A. (2017). The Yucatan minipig temporomandibular joint disc structure-function relationships support its suitability for human comparative studies. Tissue Engineering. Part C, Methods, 23(11), 700-709.

Voudouris, J. C., Woodside, D. G., Altuna, G., Angelopoulos, G., Bourque, P. J., Lacouture, C. Y., & Kuftinec, M. M. (2003). Condyle-fossa modifications and muscle interactions during Herbst treatment, Part 2. Results and conclusions. American Journal of Orthodontics and Dentofacial Orthopedics, 124(1), 13-29.

Weijs, W. A., Brugman, P., & Grimbergen, C. A. (1989). Jaw movements and muscle activity during mastication in growing rabbits. The Anatomical Record, 224(3), 407-416.

Xiang, T., Tao, Z. Y., Liao, L. F., Wang, S., & Cao, D. Y. (2021). Animal Models of Temporomandibular Disorder. Journal of pain research, 14, 1415–1430.

Yan, Y. B., Zhang, Y., Gan, Y. H., An, J. G., Li, J. M., & Xiao, E. (2013). Surgical induction of TMJ bony ankylosis in growing sheep and the role of injury severity of the glenoid fossa on the development of bony ankylosis. Journal of Cranio-Maxillo-Facial Surgery, 41(6), 476-486.

Yang, K., Wang, H. L., Dai, Y. M., Liang, S. X., Zhang, T. M., Liu, H., & Yan, Y. B. (2020). Which of the fibrous layer is more important in the genesis of traumatic temporomanibular joint ankylosis: The mandibular condyle or the glenoid fossa? Journal of Stomatology, Oral And Maxillofacial Surgery, 121(5), 517-522.



How to Cite

COUGO, M. C. R. .; QUEVEDO, A. S. de .; PONZONI, D. Animal models used to study the temporomandibular joint: literature review. Research, Society and Development, [S. l.], v. 10, n. 12, p. e420101220586, 2021. DOI: 10.33448/rsd-v10i12.20586. Disponível em: Acesso em: 4 mar. 2024.



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