Preliminary evaluation of Brazilian green propolis as coadjuvant of a subunit vaccine against lethal leptospirosis

Authors

DOI:

https://doi.org/10.33448/rsd-v10i12.20947

Keywords:

Leptospirosis; Recombinant vaccines; Adjuvant; Brazilian green propolis.

Abstract

Vaccination would be preferred to leptospirosis control measures. However, current vaccines are heat killed whole-cell bacterins that generate serovar specific protection and several side effects. Modern molecular assays have revealed antigens that may replace traditional whole-cell vaccines. Among these, LigB protein is surface-exposed outer membrane protein of virulent leptospires and therefore potential target of a protective immune response. Some unsuccessful attempts at using these antigens as vaccines have been reported. However, we believe that immune modulation through alternative adjuvants and co-adjuvants may overcome previous setbacks. In this light, our study aimed to evaluate the protective immune response in hamsters vaccinated with 40 µg of rLigBNI using oil adjuvant (OA), with or without green propolis (GP) as co-adjuvant. Upon a challenge, all groups immunized with rLigBNI, coupled or not with GP, were highly immunogenic and revealed statistically significant (p<0.05) protection of hamsters from lethal leptospirosis. Additional studies are being carried out to assess the optimum dose, protection against heterologous challenge, and vaccine dynamics.

References

Adler, B. (2015). Leptospira and leptospirosis. Current Topics in Microbiology and Immunology, Berlin, 387, 293.

Banskota, A. H., Tezuka, Y., & Kadot, S. (2001). Recent progress in pharmacological research of propolis. Phytotherapy Research, 15, 561-571.

CDC. Centers for Disease Control and Prevention. Leptospirosis. Available: https:// https://www.cdc.gov/leptospirosis/exposure/index.html. Acesso em: 16 Set 2021.

Cerqueira, G. M., & Picardeau, M. (2009). A century of Leptospira strain typing. Infection and Genetic Evolution, 9, 760-768.

Chu, W. H. (2006). Adjuvant effect of propolis on immunization by inactivated Aeromonas hydrophila in carp (Carassius auratus gibelio). Fish and Shelfish Immunology, 21, 113-117.

Costa, F., Hagan, J. E., Calcagno, J., Kane, M., Torgerson, P., Martinez-Silveira, M. S., Stein, C., Abela-Ridder, B., & Ko, A. I. (2015). Global morbidity and mortality of leptospirosis: A systematic review. PLoS Neglected Tropical Diseases, 9, e0003898.

Coutinho, M. L., Choy, H. A., Kelley, M. M., Matsunaga, J., Babbitt, J. T., Lewis, M. S., Aleixo, J. A. G., & Haake, D. A. (2011). A LigA three-domain region protects hamsters from lethal infection by Leptospira interrogans. PLoS Neglected Tropical Diseases, 5, e1422.

Dhakal, A. & Sbar, E. (2021). Jarisch Herxheimer Reaction. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing.

Fischer, G., Cleff, M. B., Dummer, L. A., Paulino, N., Paulino, A. S., Vilela, C. O., Campos, F., Storch, T., Vargas, G., Hübner, S. O., & Vidor, T. (2007). Adjuvant effect of green propolis on humoral immune response of bovines immunized with bovine herpesvirus type 5. Veterinary immunology and immunopathology, 116, 79-84.

Fischer, G., Conceição, F. R., Leite, F. P. L., Dummer, L. A., Vargas, G., Hübner, S. O., Dellagostin, O. A., Paulino, N., Paulino, A. S., & Telmo Vidor (2007). Immunomodulation produced by a green propolis extract on humoral and cellular responses of mice immunized with SuHV-1. Vaccine, 25, 1250-1256.

Hartskeerl, R. A., Collares-Pereira, M., & Ellis, W. A. (2011). Emergence, control and re-emerging leptospirosis: dynamics of infection in the changing world. Clinical Microbiology and Infection, 17, 494-501.

Haake, D. A., & Levett, P. N. (2015). Leptospirosis in humans. Current Topics in Microbiology and Immunology, 387, 65-97.

Haake, D. A. & Matsunaga, J. (2021). Leptospiral Immunoglobulin-Like Domain Proteins: Roles in Virulence and Immunity. Forntiers in immunology, 11, 579907.

Ko, A. I., Goarant, C., & Picardeau, M. (2009). Leptospira: the dawn of the molecular genetics era for an emerging zoonotic pathogen. Nature Reviews in Microbiology, 7, 736-747.

O’Hagan, D. T., MacKichan, M. L., & Singh, M. (2001). Recent developments in adjuvants for vaccines against infectious diseases. Biomolecular Enginering, 18, 69-85.

Paulino, N., Scremin, F. M. & Raichaski, L. B. (2002). Mechanisms involved in the relaxant action of the ethanolic extract of propolis in the guinea-pig trachea in vitro. Journal of Pharmacology, 54, 1–9.

Salatino, A., Fernandes-Silva, C. C., Righi, A. A., & Salatino, M. L. F. (2011). Propolis research and the chemistry of plant products, Natural Product Reports, 28, 925-036.

Sforcina, J. M., & Bankova, V. (2011). Propolis: Is there a potential for the development of new drugs?. Journal of Ethonopharmacology, 133, 253-260.

Silva, É. F., Medeiros, M. A., McBride, A. J. A., Matsunaga, J., Esteves, G. S., Ramos, J. G. S., Santos, C. S., Croda, J., Homma, A., Dellagostin, O. A., Haake, D. A., Reis, M. G., & Ko, A. I. (2007). The terminal portion of leptospiral immunoglobulin-like protein LigA confers protective immunity against lethal infection in the hamster model of leptospirosis. Vaccine, 25, 6277-6286.

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Published

29/09/2021

How to Cite

FAGUNDES, M. Q.; SEIXAS NETO, A. C. P. .; FELIX, S. R. .; VASCONCELLOS, F. A.; FISCHER, G. .; MEDEIROS, M. A. .; SILVA, Éverton F. da . Preliminary evaluation of Brazilian green propolis as coadjuvant of a subunit vaccine against lethal leptospirosis. Research, Society and Development, [S. l.], v. 10, n. 12, p. e521101220945, 2021. DOI: 10.33448/rsd-v10i12.20947. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/20947. Acesso em: 24 feb. 2024.

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Section

Health Sciences