Effect of nutritional and physical parameters on cell growth and pigment production of Serratia marcescens isolated from a legume nodule from Amazonia

Authors

DOI:

https://doi.org/10.33448/rsd-v10i13.21407

Keywords:

Soil bacteria; Biopigments; Microbial metabolism.

Abstract

The bacterium Serratia marcescens is one of the species that produces prodigiosin, a red pigment with characteristics of interest in the pharmaceutical, food and textile areas. The aim of this study was to evaluate the effect of different nutritional parameters, and identify those that positively influence cell growth and pigment production in a species of Serratia marcescens isolated from a legume nodule. Initially, the tests were carried out in submerged fermentation using Luria Bertani broth (LB), being modified for the evaluation of different sources of carbon, nitrogen, temperatures and pHs. Sample readings were taken between 24 and 72 hours, measuring cell growth by optical density (OD) at 600 nm in a 96-well microplate and pigment production was performed by extracting the metabolite with the aid of methanol and subsequent reading in a spectrophotometer at 470 nm. As a result, the highest biomass production was obtained using sucrose and glucose as carbon sources, NH4Cl as nitrogen source, at a temperature of 25 °C and pH varying between 6.0 and 7.0. The highest pigment production occurred when soluble starch was used as a carbon source and NH4Cl as a nitrogen source, at a temperature of 25 °C and pH 8.0. We conclude that from the results obtained by evaluating the different nutritional parameters, it was observed that carbon sources such as sucrose and glucose stimulate the cell growth of Serratia marcescens but significantly inhibit the production of pigments, as well as the variation in pH levels.

Author Biographies

José Carlos Ipuchima da Silva, Universidade do Estado do Amazonas

Bachelor's Degree in Biomedicine from Centro Universitário do Norte (2018), Degree in Biological Sciences from Centro Universitário Leonardo da Vinci (2020), Specialist in Clinical Analysis and Microbiology from Faculdade do Vale Elvira Dayrel (2020) and Master in Biotechnology from Graduate Program in Biotechnology and Natural Resources of the Amazon by the University of the State of Amazonas (PPGMBT-UEA).

Ana Carolina Monroy Humphrey, Universidade do Estado do Amazonas

Graduated in Biochemistry and Microbiology - Universidad del Valle de Guatemala (2012) and Master in Biotechnology and Natural Resources of the Amazon by the University of the State of Amazonas (2016). He has experience in the field of Food Science and Technology, with an emphasis on microbiological and physicochemical analysis of foods and good manufacturing practices.

Suziane Pinto Rodrigues, Universidade do Estado do Amazonas

Graduated in Biomedicine from the Uninorte Laureate International Universities School of Health Sciences (2018). She has a PAIC Scientific Initiation at Alfredo da Matta Foundation, working in research involving topics such as Genetics, Polymorphisms and Molecular Biology of microorganisms (2018). Currently, she has a master's degree in progress at the Multi-Institutional Program in Biotechnology at the Federal University of Amazonas.

Janaina Maria Rodrigues dos Santos, Instituto Nacional de Pesquisa da Amazônia

Bachelor in Chemical Engineering from CEULM/ULBRA (2011), Master in Chemical Engineering - Process and Product from the Faculty of Engineering of the University of Porto (FEUP, 2013) and PhD in Environmental Agrobiology from the Universidad Pública de Navarra (UPNA, 2018). She has experience in Research and Development, quality and the environment, having already worked with biofuels, essential oils and fertilizers.

Luiz Antonio de Oliveira, Instituto Nacional de Pesquisa da Amazônia

Degree in Agricultural Sciences from UNESP Júlio de Mesquita Filho (1976), Masters in Soil Sciences from the Federal University of Rio Grande do Sul (1982) and Doctorate in Soil Science (microbiology) from the University of Minnesota (1988). Senior Researcher at the National Institute for Research in the Amazon since January 1977.

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Published

17/10/2021

How to Cite

SILVA, J. C. I. da; HUMPHREY, A. C. M. .; RODRIGUES, S. P.; SANTOS, J. M. R. dos .; OLIVEIRA, L. A. de. Effect of nutritional and physical parameters on cell growth and pigment production of Serratia marcescens isolated from a legume nodule from Amazonia. Research, Society and Development, [S. l.], v. 10, n. 13, p. e394101321407, 2021. DOI: 10.33448/rsd-v10i13.21407. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/21407. Acesso em: 8 dec. 2021.

Issue

Section

Agrarian and Biological Sciences