Actividad antimicrobiana de hongos endofíticos aislados de Brugmansia suaveolens Bercht. & J. Presl

Authors

DOI:

https://doi.org/10.33448/rsd-v10i14.21646

Keywords:

Endophytic fungi; Brugmansia suaveolens; endophytic fungi; Elicitation; elicitation; Brugmansia suaveolens Bercht. & J. Presl.

Abstract

Natural products produced from endophyte fungi have a broad spectrum of biological activity and can be grouped into several categories. Antimicrobials constitute an important group of therapeutic agents, which can be produced and obtained from living organisms. The main objective of this study is to investigate the endophytic fungi and the leaves of Brugmansia suaveolens Bercht. & J. Presl as elicitors, aiming at the production of bioactive substances with antimicrobial properties. The extracts were prepared from 19 fungi isolated from the leaves of B. suaveolens Bercht. & J. Presl, used as a biotic elicitor. The extracts were obtained by fermentation in a submerged medium with the addition of leaves from its dry leaves, and the antimicrobial activity of its extracts was evaluated using the agar diffusion method. The results showed that 57% of the endophytic fungi showed antimicrobial activity after fermentation with the elicitor. It was observed that the antimicrobial activity was increased compared to control cultures. The leaves of B. suaveolens Bercht. & J. Presl presented bioactives that stimulated the production of a substance with antimicrobial activity by endophytic fungi, the study of such compounds showed promise to clarify the application potential of these bioactives.

References

Wu, F & Tanksley, S. D. (2010) Chromosomal evolution in the plant Family Solanaceae. Bmc Genomics 11, 182. PMID: 20236516 PMCID: PMC2847972 DOI: 10.1186/1471-2164-11-182

Bauer A.W., Kirby W.M.M., Sherris J.C. (1996). Turck M. Antibiotic susceptibility testing by standardized single method. Am. J. Clin. Pathol. 1996, 45:493-496. PMID: 5325707.

Broecling, C.D. Huhman, D. V. Farag, M. A. Smith, J. T. May, G. D. Mendes, P. Dixon, R. A. Summer L. W. (2005). Metabolic profiling of Medicago truncatula cell cultures reveals the effects of biotic and abiotic elicitors on metabolism. J Exp Bot, 56(410): 323-36. PMID: 15596476 DOI: 10.1093 / jxb / eri058.

Guimarães, D. O.; Da Silva. Momesso, L.; Pupo, M. T. (2010). Antibióticos: Importância Terapêutica e Perspectivas para a Descoberta e Desenvolvimento de Novos Agentes. Química Nova, v. 33, n. 3, p. 667-679. https://doi.org/10.1590/S0100-40422010000300035

Qian, Z.G. Zhao, H. J. Xu, Y. Qian, X. Zhong J. J. (2006). Novel chemically synthesized salicylate derivative as an effective elicitor for inducing the biosynthesis of plant secondary metabolites. Biotechnol Prog. 22(1): 331-3. PMID: 16454528 DOI: 10.1021 / bp0502330.

Oliveira, R.B.; Pires S.A. G. Costa, F.B. (2003). In: Plantas tóxicas. Conhecimento e prevenção de acidentes. Ed. Holos, p. 34-37.

Otero, J.M.; Nielsen, J. (2010). Industrial systems biology. Biotechnol. Bioeng, 105, 439-460. https://doi.org/10.1002/bit.22592

Ribeiro, I. Ribeiro, M. H. L (2008). Naringin and naringenin determination and control in grapefruit juice by a validated HPLC method. Food Control. v.19, n.4, p.432-438. https://doi.org/10.1016/j.foodcont.2007.05.007

Simões, C.M.O.; Schenkel, E.P.; Gosmann, G.; Mello, J.C.P. De; Mentz, L.A. Petrovick, P.R. (1999). Farmacognosia da planta ao medicamento. Porto Alegre: UFRGS e UFSC, 821p.

Strobel GA, Daisy B, Castillo U. Harper J. (2004). Natural products from endophytic microrganisms. J Nat Prod, 67(2):257–268. PMID: 14987067 DOI: 10.1021 / np030397v.

Ting, D., Wei, S.W., Xia, Q.Y. Yang, J.H. (2013). Studies on some active components and antimicrobial activities of the fermentation broth of endophytic fungi DZY16 isolated from Eucommia ulmoides oliv, Afr. J. Biotechnol. 12 15. DOI: 10.5897 / AJB11.2638

Yoshikawa, M. Yamaoka, N. Takeuchi, Y. (1993). Elicitors: Their significance and primary modes of action in the induction of plant defense reactions. Plant Cell Physiol, 34: 1163-1173. https://doi.org/10.1093/oxfordjournals.pcp.a078537

Zhang H.W., Song Y.C. Tan R.X. (2006). Biology and chemistry of endophytes. Nat Prod Rep 23:753–771. PMID: 17003908 DOI: 10.1039 / b609472b

Zheng, W.; Wang, S.Y. (2001). Antioxidant activity and phenolic compounds in selected herbs. J. Agric. Food Chemistry. Chicago: v.49, p. 5165-5170. PMID: 11714298 DOI: 10.1021 / jf010697n

Radman, R. Saez, T. Bucke, C. Keshavarz T. (2003). Elicitation of plant and microbial systems. Biotechnol Appl Biochem. 37: 91-102. PMID: 12578556 DOI: 10.1042 / ba20020118

Gao F. Yong Y. Dai. C. (2011). Effects of endophytic fungal elicitor on two kinds of terpenoids production and physiological indexes in Euphorbia pekinensis suspension cells. J. Med. Plant. Res. Vol. 5(18), pp. 4418-4425. https://doi.org/10.5897/JMPR.9000519

NCSLI Clinical and laboratory standards institute. (2011). Performance standards for antimicrobial susceptibility testing, 21st informational supplement. CLSI document M100-S21. Clinical and Laboratory Standards Institute, Wayne, PA, 2011. ISBN 1-56238-742-1

LIU, D. Z.; LIANG, B. W.; LI, X. F.; LIU, Q. (2014). Induced production of new diterpenoids in the fungus Penicillium funiculosum. Natural Product Communications, v. 9, n. 5, p. 607 - 608. https://doi.org/10.1177/1934578X1400900502

Salvador, M.J.; Ferreira, E.O.; Pral, E.M.F.; Alfieri, S.C.; Albuquerque, S.; Ito, I. Y. & Dias, D.A. (2002). Bioactivity of crude extracts and some constituents of Blutaparon portulacoides (Amaranthaceae). Phytomedicine, 9 (6), 566 - 571. PMID: 12403168 DOI: 10.1078 / 09447110260573227

Candido, R.C.; Toloi, M.R.T.; Frnaceschini, S.A.; Garcia, F.R. & Minto, E.C.M. (1998). In vitro activity of antimycotic agents determined by E-test method against vagina shape Candid a species. Mycopathologia. 144 (1), 15 - 20. PMID: 10422269 DOI: 10.1023 / a: 1006928628550

Penna, C.; Marinos, S.; Vivot, E.; Cruañes, M.C.; Munoz, J.D.; Cruanes, J.; Ferraro, G.; Gutkind, G. & Martino, V. (2001). Antimicrobial activity of Argentine plants used in the treatment of infectious diseases. Isolation of active compounds from Sebastiania brasiliensis. Journal of Ethnopharmacology, 77 (1), 37 – 40. PMID: 11483376 DOI: 10.1016 / s0378-8741 (01) 00266-5

Nogueira, L.G. (2009). Determinação do potencial biológico e antioxidante de extratos de casca de raiz de Maytenus ilicifolia (Celastraceae). 88p. Masters dissertation, Araraquara. Universidade Estadual Paulista. “Júlio de Mesquita Filho”. Faculty of Pharmaceutical Sciences.

Bracjet, A.; Mateus, L., Cherkaooui, S.; Christen, P.; Gauvrit, J.-Y.; Lantéri, P.; Veuthey, J.-L. (1999). Apllication of central composite designs in the supercritical fluid extraction of tropane alkaloids in plant extracts. Analysis, v. 27, p. 772- 778. DOI: 10.1051 / analusis: 1999143

Cusido, R.M.; Palazón, J.; Piñol, M.T.; Bonfill, M.; Morales, C. (1999). Datura metel: in vitro production of tropane alkaloids. Planta Medica, v.65, p. 144- 148. PMID: 10193206 DOI: 10.1055 / s-1999-13976

Azevedo, J. L.; Costa, S. O. P. (1973). Exercícios Práticos de Genética. Companhia Editora Nacional – EDUSP, São Paulo-SP. 288 p.

Castellani, A. (1939). Viability of Mold Culture of Fungi in Distilled Water. J. Trop. Med. Hyg. 42: 225

Galvão, R. M. S. (1998). Variabilidade Genética Detectada por RAPD em Glomerella cingulata, um dos fungos endofíticos mais frequentes, isolados de Theobroma grandiflorum, Pueraria phaseoloides e Scleria pterota. Masters dissertation, UFSCar/UFAM. Manaus, Amazonas. 151p.

Li, J.Y.; Harper, J. K.; Grant, D. M.; Tombe, B. O.; Bashyal, B.; Hess, W. M.; Strobel, G. A. (2001). Ambuic Acid, a Highly Functionalized Cyclohexenone with Antifungal Activity from Pestalotiopsis spp. and Monochaetia sp. Phytochemistry, 56(5): 463-468. PMID: 11261579 DOI: 10.1016 / s0031-9422 (00) 00408-8

Li, J.Y; Strobel, G.A. (2001). Jesterone and Hydroxy-Jesterone Antioomycete Cyclohexenone Epoxides From the Endophytic Fungus Pestalotiopsis jesteri. Phytochemistry, 57(2): 262-265. PMID: 11382242 DOI: 10.1016 / s0031-9422 (01) 00021-8.

Stamford, T. L. M.; Araújo, J. M.; Stamford, N. P. (1998). Atividade enzimática de microrganismos isolados do Jacatupé (Pachyrhizus erosus L. Urban). Ciênc. Tecnol. Aliment.18(4): 382-385. https://doi.org/10.1590/S0101-20611998000400004

Kleinkauf, H.; Von Dohren, H. (1990). Biosynthesis of Peptide Antibiotics. European Journal of Biochemistry, 192: 1-15. PMID: 2205497 DOI: 10.1111 / j.1432-1033.1990.tb19188.x

Alexopoulos, C.J.; Mims, C.W. (1996). Introductory Mycology. 4th edition. John Wiley & Sons INC, USA. 869p. ISBN: 978-0-471-52229-4

Demain, A. (1992). Microbial Secondary Metabolism: a New Opportunity for Industry. Ciba Foundation Symposium. 3-23. PMID: 1302184 DOI: 10.1002 / 9780470514344.ch2

Trilli, V.; Michilini, V.; Montovani, V.; Pirt, S.J. (1978). Development of the agar disk method for the rapid selection of cephalosporim producers with improved yields. Antimicrobial Agents and Chemotherapy, 13(1): 7-13. DOI: https://doi.org/10.1128/AAC.13.1.7

Bach, E.E.; Kimati, H. (1999). Purification and Characterization of Toxins from Wheat Isolates of Drechslera tririci-repentis, Bipolaris bicolor, and Bipolaris sorokiniana. Journal of Venomous Animals and Toxins, 5(2): 184-199. DOI: https://doi.org/10.1590/S0104-79301999000200006

Wang, J.; Li, G.; Lu, H.; Zheng, Z.; Huang, Y.; Su, W. (2000). Taxol from Tubercularia sp. strain TF5, an endophytic fungus of Taxus mairei. FEMS-Microbiology Letters, 193: 249-253. DOI: https://doi.org/10.1111/j.1574-6968.2000.tb09432.x

Rodrigues, K. F.; Hesse, M.; Werner, C. (2000). Antimicrobial activities of secondary metabolites produced by endophytic fungi from Spondias mombin. Journal Basic Microbial, 40(4): 261-267. PMID: 10986672 DOI: 10.1002 / 1521-4028 (200008) 40: 4 <261 :: AID-JOBM261> 3.0.CO; 2-D

El-Hawary, S. S.; Mohammed, R.; AbouZid, S. F.; Bakeer, W.; Ebel, R.; Sayed, A. M.; Rateb, M. E. (2016). Solamargine production by a fungal endophyte of Solanum nigrum. Journal of Applied Microbiology, 120, 900—911. PMID: 26811095 DOI: 10.1111 / jam.13077

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Published

27/10/2021

How to Cite

COSTA, S. P. da .; SCHUENCK-RODRIGUES, R. A.; CARDOSO, V. da S. .; VALVERDE, S. S.; VERMELHO, A. B. .; RICCI-JÚNIOR, E. . Actividad antimicrobiana de hongos endofíticos aislados de Brugmansia suaveolens Bercht. & J. Presl. Research, Society and Development, [S. l.], v. 10, n. 14, p. e113101421646, 2021. DOI: 10.33448/rsd-v10i14.21646. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/21646. Acesso em: 14 nov. 2024.

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Health Sciences