Study of the influence of the lactation stage on the concentration of the main fatty acids in lactating women with full-term infants




Human milk; Lactation; Fatty acid; CG-FID.


The present study aims to evaluate the influence of the stages of lactation (colostrum, transition and mature) on the composition of the main fatty acids and sums of fatty acids in human milk from lactating women with full-term infants. For this, approximately 5 ml of human milk was collected individually, in the same period of the day, and after 1 hour of the breast being emptied, through manual milking, then the fatty acid composition was performed by means of chromatography in gas phase coupled to flame ionization detector (CG-DIC). The fatty acid found mostly for colostrum was linoleic acid (18:2n-6), whereas for transition and mature milk it was oleic acid (18:1n-9), in addition to all essential fatty acids and strictly essential were identified and quantified in the three phases. It was possible to observe that the lactation stage directly influences the fatty acid composition, with colostrum milk being the one with the greatest distinction between the lactation phases, and transitional milk and mature milk having more similarity to each other.


Alves, E. S., Castro, M. C., Saqueti, B. H., Manin, L. P., Silveira, R. D., Souza, P. M., ... & Visentainer, J. V. (2021). Whey Isolation from Rejected Human Milk and Its Lipid Content Characterization by GC-FID and ESI-MS. Journal of the Brazilian Chemical Society, 32, 1884-1894.

Ballard, O., & Morrow, A. L. (2013). Human milk composition: nutrients and bioactive factors. Pediatric Clinics, 60, 49-74.

Binns, C., Lee, M., & Low, W. Y. (2016). The long-term public health benefits of breastfeeding. Asia Pacific Journal of Public Health, 28, 7-14.

Bourlieu, C., & Michalski, M. C. (2015). Structure–function relationship of the milk fat globule. Current Opinion in Clinical Nutrition & Metabolic Care, 18, 118-127.

Demmelmair, H., & Koletzko, B. (2018). Lipids in human milk. Best Practice & Research Clinical Endocrinology & Metabolism, 32, 57-68.

Folch, J., Lees, M., & Stanley, G. S. (1957). A simple method for the isolation and purification of total lipides from animal tissues. Journal of biological chemistry, 226, 497-509.

Gurnida, D. A., Rowan, A. M., Idjradinata, P., Muchtadi, D., & Sekarwana, N. (2012). Association of complex lipids containing gangliosides with cognitive development of 6-month-old infants. Early human development, 88, 595-601.

International Organization for Standardization – ISO. 12966:2017. Animal and vegetable fats and oils — Gas chromatography of fatty acid methyl esters — Part 2: Preparation of methyl esters of fatty acids.

Johnston, M., Landers, S., Noble, L., Szucs, K., & Viehmann, L. (2012). Breastfeeding and the use of human milk. Pediatrics, 129, e827-e841


Levy, L., & Tedstone, A. (2017). UK dietary policy for the prevention of cardiovascular disease. In Healthcare. Multidisciplinary Digital Publishing Institut. 5, 9.

Manin, L. P., Rydlewski, A. A., Galuch, M. B., Pizzo, J. S., Zappielo, C. D., Senes, C. E., ... & Visentainer, J. V. (2019). Evaluation of the lipid quality of lyophilized pasteurized human milk for six months by GC-FID and ESI-MS. Journal of the Brazilian Chemical Society, 30, 1579-1586.

Manin, L. P., Rydlewski, A. A., Alves, E. S., Ponhozi, I. B., Castro, M. C., Saqueti, B. H. F., ... & Visentainer, J. V. (2020). Evaluation of the lipid composition of the three lactation phases of raw, pasteurized and lyophilized pasteurized human milk. Research, Society and Development, 9, e26891211136-e26891211136.

Mehrotra, V., Sehgal, S. K., & Bangale, N. R. (2019). Fat structure and composition in human milk and infant formulas: Implications in infant health. Clinical Epidemiology and Global Health, 7, 153-159.

Organização Mundial da Saúde. (2003). Global Strategy on Diet, Physical Activity and Health: Diet, Nutrition and the Prevention of Chronic Diseases.

Pereira, A. S., Shitsuka, D. M., Parreira, F. J., & Shitsuka, R. (2018). Metodologia da pesquisa científica. [eBook]. Santa Maria. Ed. UAB / NTE / UFSM.

Prell, C., & Koletzko, B. (2016). Breastfeeding and complementary feeding: recommendations on infant nutrition. Deutsches Ärzteblatt International, 113, 435.

Rydlewski, A. A., Pizzo, J. S., Manin, L. P., Zappielo, C. D., Galuch, M. B., Santos, O. O., & Visentainer, J. V. (2020). Métodos analíticos utilizados para a determinação de lipídios em leite humano: uma revisão. Revista Virtual de Química, 12.

Rydlewski, A. A., Silva, P. D., Manin, L. P., Tavares, C. B., Paula, M. G., Figueiredo, I. L., ... & Visentainer, J. V. (2019). Lipid Profile Determination by Direct Infusion ESI-MS and Fatty Acid Composition by GC-FID in Human Milk Pools by Folch and Creamatocrit Methods. Journal of the Brazilian Chemical Society, 30, 1063-1073.

Simionato, J. I., Garcia, J. C., Santos, G. T. D., Oliveira, C. C., Visentainer, J. V., & Souza, N. E. D. (2010). Validation of the determination of fatty acids in milk by gas chromatography. Journal of the Brazilian Chemical Society, 21, 520-524.

Simopoulos, A. P. (2004). Omega-6/omega-3 essential fatty acid ratio and chronic diseases. Food reviews international, 20, 77-90.

Van Gysel, M., Cossey, V., Fieuws, S., & Schuermans, A. (2012). Impact of pasteurization on the antibacterial properties of human milk. European journal of pediatrics, 171, 1231-1237.

Vyssotski, M., Bloor, S. J., Lagutin, K., Wong, H., & Williams, D. B. G. (2015). Efficient separation and analysis of triacylglycerols: quantitation of β-palmitate (OPO) in oils and infant formulas. Journal of agricultural and food chemistry, 63, 5985-5992.

Wang, L., Li, X., Liu, L., da Zhang, H., Zhang, Y., Chang, Y. H., & Zhu, Q. P. (2020). Comparative lipidomics analysis of human, bovine and caprine milk by UHPLC-Q-TOF-MS. Food chemistry, 310, 125865.

Zhao, P., Zhang, S., Liu, L., Pang, X., Yang, Y., Lu, J., & Lv, J. (2018). Differences in the triacylglycerol and fatty acid compositions of human colostrum and mature milk. Journal of agricultural and food chemistry, 66, 4571-4579.



How to Cite

CASTRO , M. C.; ALVES, E. S.; SAQUETI, B. H. F.; FERREIRA, C. S. R.; ALVES, J. S.; COSTA, J. C. M. .; SANTOS, O. de O.; VISENTAINER, J. V. Study of the influence of the lactation stage on the concentration of the main fatty acids in lactating women with full-term infants. Research, Society and Development, [S. l.], v. 10, n. 14, p. e308101422174, 2021. DOI: 10.33448/rsd-v10i14.22174. Disponível em: Acesso em: 14 jun. 2024.



Agrarian and Biological Sciences