Impact of doppler ultrasound on the clinical and andrological stallions examination
DOI:
https://doi.org/10.33448/rsd-v10i14.22412Keywords:
Fertility; Resistive index; Sperm membranes.Abstract
This study aimed to evaluate the association of Doppler ultrasound with clinical andrological examination to identify the reproductive quality of stallions in paraiban swamp region. Eleven horses were used, aged between three and 27 years. The ejaculates were collected by the artificial vagina method and the seminal samples were submitted to field evaluation (subjective motility, cellular integrity) and in the laboratory (objective motility, cellular integrity). After seminal collection, the animals were submitted to testicular blood flow evaluation with Doppler ultrasound. Differences (P<0.05) were observed between the parameters of subjective motility, field-evaluated, and objective motility, in the laboratory. The integrity of the sperm plasma membrane was similar in field and laboratory tests. In the ultrasound parameters, it was observed that the Pulsatility Index has a positive correlation with lateral head displacement amplitude (ALH) of the spermatozoa. Finally, when evaluating age parameter, elderly animals presented reduced motility and lower blood supply in the testicular region, resulting in low fertility. It is concluded that the clinical andrological examination, when isolated, does not determine the fertile capacity of stallions; doppler-associated ultrasound identifies subfertile animals and can be an auxiliary tool in the stallions selection.
References
Agência Executiva de Gestão das Águas do Estado da Paraíba – AESA. (2017). Climatologia. http://www.aesa.pb.gov.br/aesa-website/meteorologiachuvas/climatologia.
Alvarenga, M. A., & Papa, F. O. (2009) Principais distúrbios reprodutivos observados em garanhões no Brasil. Revista Brasileira de Reprodução Animal, 6, 204-209. http://www.cbra.org.br/pages/publicacoes/rbra/download/p204-209.pdf
Arruda, R. P., Silva, D. F., Alonso, M. A., Andrade, A. F. C.; Nascimento, J., Gallego, A. M., Martins, S. M. M. K., & Granato, T. M. (2010) Nutraceuticals in reproduction of bulls and stallions. Revista Brasileira de Zootecnia, 39, 393-400. https://doi.org/10.1590/S1516-35982010001300043
Carvalho, J.O.; Sartori, R.; Lemes, A.P., Mourão, G. B., & Dode, M A. N. (2009) Cinética de espermatozoides criopreservados de bovinos após sexagem por citometria de fluxo. Pesquisa Agropecuária Brasileira, 44(10), 1346-1351. https://doi.org/10.1590/S0100-204X2009001000019
CBRA – Colégio Brasileiro de Reprodução Animal (2013). Manual para Exame Andrológico e Avaliação de Sêmen Animal. Third ed. CBRA, Belo Horizonte. 46p.
Coelho, R. W. de A., & Dias, J. C. O. (2021). Congelação de sêmen equino após 24 horas de resfriamento. Research, Society and Development, 10(1), e1831019939. https://doi.org/10.33448/rsd-v10i1.9939
Cunha, M. S., Bonato, D. V., Taira, A. R., Teixeira, P. P. M. Degeneração testicular em machos: dos animais ao homem. Investigação, 14(6):54-61, 2015. https://doi.org/10.26843/investigacao.v14i6.912.
Farrell, P. B., Foote, R. H., McArdle, M. M., Trouern-Trend, V. L., & Tardif, A. L. (1996). Media and dilution procedures tested to minimize handling effects on human, rabbit, and bull sperm for computer-assisted sperm analysis (CASA). Journal of Andrology, 17(3), 293–300.
Ginther, O. J. (1992). Reproductive biology of the mare (Basic and applied aspects). 2nd ed. Cross Plains: Equiservices, 642p.
Hammes, A. M.; Pimentel, C. A.; Fernandes, C. E. (1996). Fertilidades em garanhões avaliadas através do exame andrológico. Ciência Rural, 26, 277-283. https://www.scielo.br/j/cr/a/CQmcSRnR3qY3Rv7w53qynbP/?format=pdf&lang=pt
Januskauskas, A., Johannisson, A., & Rodriguez-Martinez, H. (2003). Subtle membrane changes in cryopreserved bull semen in relation with sperm viability, chromatin structure, and field fertility. Theriogenology, 60(4), 743–758. https://doi.org/10.1016/s0093-691x(03)00050-5
Linhares, R. S., & Sousa, M. S. de. (2020). Uma perspectiva jurídica sobre a relevância socioeconômica e cultural da vaquejada e seus reflexos no direito dos animais. Revista do programa de pós-graduação em gestão ambiental no semiárido, 2(1), 01-20. https://www.gvaa.com.br/revista/index.php/Regeas/article/view/7984
Nery, I., Araújo Silva, R., Souza, H. M., Arruda, L., Monteiro, M. M., Seal, D., Silva, G. R., Silva, T., Carneiro, G. F., Batista, A. M., Câmara, D. R., & Guerra, M. (2020). Effects of L-Carnitine on Equine Semen Quality During Liquid Storage. Biopreservation and Biobanking, 18(5), 403–408. https://doi.org/10.1089/bio.2020.0025
Obino Filho, F. (2016) Palavra do Presidente. In: Lima, R.A.S.; & Cintra, A.G. Revisão do Estudo do Complexo do Agronegócio do Cavalo. Assessoria de Comunicação e Eventos do Ministério da Agricultura, Pecuária e Abastecimento. https://www.gov.br/agricultura/pt-br/assuntos/camaras-setoriais-tematicas/documentos/camaras-setoriais/equideocultura/anos-anteriores/revisao-do-estudo-do-complexo-do-agronegocio-do-cavalo/view.
Ortega-Ferrusola, C., Gracia-Calvo, L. A., Ezquerra, J., & Pena, F. J. (2014). Use of colour and spectral Doppler ultrasonography in stallion andrology. Reproduction in domestic animals = Zuchthygiene, 49 Suppl 4, 88–96. https://doi.org/10.1111/rda.12363
Papa, F. O., Alvarenga, M. A., Dell'qua, J.; Monteiro, G. M. (2007) Manual de andrologia e manipulação de sêmen equino. São Paulo: Botupharma, 60p.
Pavlica, P., & Barozzi, L. (2001). Imaging of the acute scrotum. European Radiology, 11(2), 220–228. https://doi.org/10.1007/s003300000604
Pereira, E. L., Bataglion, G. A., & Mazo, J. Z. (2020) Equoterapia, saúde e esporte: figurações da prática no Rio Grande do Sul, 1970-2000. História, Ciências, Saúde-Manguinhos, 27(3) 879-897. https://doi.org/10.1590/S0104-59702020000400010.
Pimentel, M. M. L., Santos, F. A. Dos, Dias, R. V. C., Macêdo, L. B. De, Fonseca, Z. A. A. De S., André, W. P. P., & Ribeiro, W. L. C. (2014). Monitoramento do ciclo estral de fêmeas equinas por meio de citologia vaginal, ultrassonografia e dosagem hormonal. Arquivos de Ciências Veterinárias e Zoologia da UNIPAR, 17(1), 69-75. https://doi.org/10.25110/arqvet.v17i1.4920
Pintado, B., de la Fuente, J., & Roldan, E. R. (2000). Permeability of boar and bull spermatozoa to the nucleic acid stains propidium iodide or Hoechst 33258, or to eosin: accuracy in the assessment of cell viability. Journal of Reproduction and Fertility, 118(1), 145–152. https://pubmed.ncbi.nlm.nih.gov/10793636/
Roser J. F. (2001). Endocrine and paracrine control of sperm production in stallions. Animal Reproduction Science, 68(3-4), 139–151. https://doi.org/10.1016/s0378-4320(01)00151-8
Schurich, M., Aigner, F., Frauscher, F., & Pallwein, L. (2009). The role of ultrasound in assessment of male fertility. European Journal of Obstetrics, Gynecology, and Reproductive Biology, 144 Suppl 1, S192–S198. https://doi.org/10.1016/j.ejogrb.2009.02.034
Sieme, H., Oldenhof, H., & Wolkers, W. F. (2015). Sperm Membrane Behaviour during Cooling and Cryopreservation. Reproduction in domestic animals = Zuchthygiene, 50 Suppl 3, 20–26. https://doi.org/10.1111/rda.12594
Smith, A. M.; Staniar, W. B.; & Splan, R. K. Associations between yearling body measurements and career racing performance in Thoroughbred racehorses. Journal of Equine Veterinary Science, 26(5), 212–214. https://doi.org/10.1016/j.jevs.2006.03.002
Snoeck, P. P. d. N., Melo, M. I. V. d., Alves, S. G. G., Bittencourt, R. F., Filho, A. d. L. R., Chalhoub, M., & Henry, M. (2014). Qual é o teste hiposmótico mais indicado para avaliar a integridade funcional de espermatozoides equino criopreservados? Brazilian Journal of Veterinary Medicine, 36(4), 335-361. http://rbmv.org/index.php/BJVM/article/view/562
Verstegen, J., Iguer-Ouada, M., & Onclin, K. (2002). Computer assisted semen analyzers in andrology research and veterinary practice. Theriogenology, 57(1), 149–179. https://doi.org/10.1016/s0093-691x(01)00664-1
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2021 Allan Gledson Ferreira dos Santos; Hugo Albino Braz; Robespierre Augusto Joaquim Araújo Silva; Maria Madalena Pessoa Guerra; Marlon de Vasconcelos Azevedo; Sildivane Valcácia Silva
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
1) Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
2) Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
3) Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.