Antioxidant activity of Myrciaria dubia (camu-camu) extracts Myrtaceae

Authors

DOI:

https://doi.org/10.33448/rsd-v11i2.25130

Keywords:

Camu-camu; Antioxidant; Hydroalcoholic extract; Glycolic extract.

Abstract

The fruit of Myrciaria dubia is rich in bioactives, these have a high content of ascorbic acid and phenolic compounds, for this reason, the fruit has been gaining prominence for its antioxidant capacity. The aim of this study was to evaluate whether different extracts obtained from M. dubia fruit peels influence the antioxidant activity. 70% hydroalcoholic (EHA) and 10 and 20% glycolic extracts (EG10 and EG20) were obtained from bark from a moderately coarse powder with loss on desiccation of 13.53%, total ash of 1.95%, ash insoluble in acids of 0.057%, pH 2.53±0.05 and swelling index of 1.1ml. The extracts were characterized as to the content of ascorbic acid and total polyphenols and the antioxidant activity determined by DPPH and ABTS+. The EHA showed 7011±39 mg/100ml of ascorbic acid and 7.75% of total polyphenols, while in the EG10 and EG20 the ascorbic acid content was 3034±0 and 6137±0 mg/100mL and total polyphenols of 2 .65 and 3.55% respectively. The extracts showed DPPH antioxidant capacity with IC50 of 11.80, 48.95 and 25.26 µg/mL and ABTS+ of 92.91±0.205, 74.59±0.259 and 77.21±1.34% in the EHA extracts, EG10 and EG20, respectively. The extracts have important contents of bioactive compounds, however, the solvent influenced the content and biological activity, with EHA having the highest content of bioactives, which also reflected in its greater antioxidant capacity.

References

Abanto-Rodriguez, C., et al. (2016). Relação entre os nutrientes minerais e o teor de vitamina C em plantas camu-camu (Myrciria dubia) cultivadas em solos altos e solos de inundação de Ucayali, Peru. Scientia Agropecuaria. Trujillo, 7 (3), 297-304. doi:10.17268/sci.agropecu.2016.03.18

Akter, M. S., et al. (2011). Nutritional compositions and health promoting phytochemicals of camu-camu (Myrciaria dubia) fruit: A review. Food Research International, 44 (7), 1728-1732. doi: 10.1016/j.foodres.2011.03.045

Alves, R. E., et al. (2002). CamuCamu ([Myrciaria dubia (H.B.K.) Mc Vaugh] Mc Vaugh): a rich natural source of vitamin C. Proc. Interam. Soc. Trop. Hortic, 46, 11–13.

Andrade, J. S., et al. (1991). Valor nutricional do camu-camu (Myrciaria dubia (H.B.K.) Mc Vaugh Myrtcaceae) cultivado em terra firme na Amazônia Central. Revista Brasileira de Fruticultura, 13 (3), 307-311.

Arruda A. S., et al. (2011). Desenvolvimento do camu-camu (Myrciaria dubia) em diferentes substratos nas condições de Ipameri-GO, Enciclopédia Biosfera, 7, 01-07.

Azwanida, N. N. (2015). A Review on the Extraction Methods Use in Medicinal Plants, Principle, Strength and Limitation. Medicinal & Aromatic Plants, 4 (3), 01-06. doi:10.4172/2167-0412.1000196

Barni, S. T. & Cechinel Filho, V. & Couto, A. G. (2009). Caracterização química e tecnológica das folhas, caules e planta inteira da Ipomoea pes-caprae (L.) R. Br., Convolvulaceae, como matéria-prima farmacêutica. Revista Brasileira de Farmacognosia, 19, 865-870. doi:10.1590/S0102-695X2009000600012

Bomfim, M. P., et al. (2017). Caracterização dos compostos bioativos em frutas e hortaliças adquiridas no comércio de Padova-Itália. Revista Iberoamericana de Tecnología Postcosecha, 18 (2), 82-92.

Brand-Williams, W. & Cuverlier, M. E.& Berset, C. (1995). User of free radical method to evaluate antionxidant activity. Lebensmittel-Wissenchaft Technologie, 28, 25-30. doi:10.1016/S0023-6438(95)80008-5

Brasil. (1999). Farmacopéia Brasileira. 4ª ed. Brasília: Agência Nacional de Vigilância Sanitária.

Brasil. (2010). Farmacopéia Brasileira. 5ª ed. Brasília: Agência Nacional de Vigilância Sanitária.

Brasil. (2019). Farmacopéia Brasileira. 6ª ed. Brasília: Agência Nacional de Vigilância Sanitária.

Castro, J. C. & Maddox, J. D. & Imán, S.A. (2018). Camu-camu - Myrciaria dubia (Kunth) McVaugh. In. Rodrigues, S.; Silva, E. O. (editores). Exotic Fruits, Academic Press, 97-105. doi:10.1016/B978-0-12-803138-4.00014-9

Chirinos, R., et al. (2010). Antioxidant compounds and antioxidant capacity of Peruvian camu-camu (Myrciaria dubia (H.B.K.) Mc Vaugh) fruit at different maturity stages. Food Chem. 120, 1019–1024. doi:10.1016/j.foodchem.2009.11.041

Conceição, N., et al. (2020). By-Products of Camu-Camu [Myrciaria dubia (Kunth) McVaugh] as Promising Sources of Bioactive High Added-Value Food Ingredients: Functionalization of Yogurts. Melecules, 25 (1),1-17. doi:10.3390/molecules25010070

Costa, A. F. (1972). Farmacognosia. Lisboa, Fundação Colouste Gulbenkian, 2 (3), 1032.

Fidelis, M., et al. (2020). From byproduct to a functional ingredient: Camu-camu (Myrciaria dubia) seed extract as an antioxidant agent in a yogurt model. Journal of dairy science, 103 (2), 1131-1140. doi:10.3168/jds.2019-17173

Fracassetti, D., et al. (2013). Derivados do ácido elágico, elagitaninos, proantocianidinas e outros fenólicos, vitamina C e capacidade antioxidante de dois produtos em pó da fruta camu-camu (Myrciaria dubia). Química alimentar, 139 (1-4), 578-588.

Fujita A., et al. (2013). Impact of spouted beddrying on bioactive compounds, antimicrobial and antioxidant activities of commercial frozen pulp of camu-camu (Myrciaria dubia Mc. Vaugh). Food Res. 54, 495-500. doi:10.1016/j.foodres.2013.07.025

Góis, F. L. (2021). Controle de qualidade e perfil metabólico de drogas vegetais associadas para o preparo de chás, comercializadas em Salvador-Bahia, Brasil.

Grigio, M. L., et al. (2021). Nutraceutical potential, quality and sensory evaluation of camu-camu pure and mixed jelly. Food Science and Technology. doi:10.1590/fst.03421

Hagerman, A. E. & Butler, L.G. (1989). Choosing apropriate methods and standards for assaying tannin. Journal of Chemical Ecology, New York, 15 (6), 1795-1810. doi:10.1007/BF01012267

Herrera-Calderon, O., et al. (2018). Phytochemical screening, total phenolic content, antioxidant, and cytotoxic activity of five peruvian plants on human tumor cell lines. Pharmacogn. Res. 10, 161-165. doi:10.4103/pr.pr_109_17

Hoelzel, S. C. S. M., et al. (2003). Estudo Morfo-Anatômico da Raiz de Waltheria douradinha Saint Hilaire (Sterculiaceae). Acta farmaceutica bonaerense, 22 (2), 113-8.

Ignat, I. & Volf, I. & Popa, V. I. (2011). A critical review of methods for characterisation of polyphenolic compounds in fruits and vegetables. Food chemistry, 126 (4), 1821-1835. doi:10.1016/j.foodchem.2010.12.026

Instituto Adolfo Lutz. (2008). Métodos físico-químicos para análises de alimentos. Normas Analíticas do Instituto Adolfo Lutz. 4, 1000.

Jerez, M., et al. (2006). Influence of extraction conditions on phenolic yields from pine bark: Assessment of procyanidins polymerization degree by thiolysis. Food Chemistry, 94 (3), 406-414. doi:10.1016/j.foodchem.2004.11.036

Lebeau, J., et al. (2000). Antioxidant properties of di-tert-butylhydroxylated flavonoids, Free Radic. Biol. Med., 29 (9), 900-912. doi:10.1016/S0891-5849(00)00390-7

Lewis, M. J. (1993). Propiedades físicas de los alimentos y de los sistemas de procesado.Zaragoza: Editorial Acribia. 39-41.

Liu, Z. H., et al. (2013). Effects of biomass particle size on steam explosion pretreatment performance for improving the enzyme digestibility of corn stover. Industrial Crops and Products, 44, 176-184. doi: 10.1016/j.indcrop.2012.11.009

Macheix, J.J. & Fleuriet, A. & Billot, J. (1990). Fruit phenolics. Boca Raton, Florida, (ed.) CRC Pres, 378. doi: 10.1080/10408399109527552

Maeda, R. N., et al. (2006). "Determinação da formulação e caracterização do néctar de camu-camu (Myrciaria dubia McVaugh)." Food Science and Technology, 26, 70-74. doi: 10.1590/S0101-20612006000100012

Melo, E. A. & Guerra, N.B. (2002). Ação antioxidante de compostos fenólicos naturalmente presentes em alimentos. Bol. SBCTA, 36 (1), 1-11.

Melo, E. A., et al. (2008). Capacidade antioxidante de frutas. Revista Brasileira de Ciências Farmacêuticas, 44, 193-201. doi:10.1590/S1516-93322008000200005

Mitra, S. & Dungan, S. R. (1997). Micellar properties of Quillaja saponin. 1. Effects of temperature, salt, and pH on solution properties. Journal of agricultural and food chemistry, 45 (5), 1587-1595. doi:10.1021/jf960349z

Mole, S. & Waterman, P.G. (1987). A critical analysis of techniques for measuring tannins in ecological studies I. Techniques dor chemically defining tannins. Oecologia, 72, 137-147. doi: 10.1007/BF00385058

Morais, L. de C. & Pinheiro, S. S. (2018). Nutrients and bioactive compounds of açaí, bacuri, buriti, camu-camu and cubiu. Tropical fruits–from cultivation to consumption and health benefits (Editors: TODOROV, SD & PIERI, FA), Nova Science Publisher, Inc, 121-132.

Navarro, F. F., et al. (2015). Quality control and phytochemical screening of the plant drug in the leaves of Morus nigra L. (MORACEAE). Journal of Basic and Applied Pharmaceutical Sciences, 36 (2), 259–265.

Neves L.C., et al. (2015). Bioactive compounds and antioxidant activity in pre-harvest camu-camu [Myrciaria dubia (H.B.K.) Mc Vaugh] fruits. Sci. Horticulturae, 186, 223- 229. doi: 10.1016/j.scienta.2015.02.031

Oliveira, V. B., et al. (2016). Efeito de diferentes técnicas extrativas no rendimento, atividade antioxidante, doseamentos totais e no perfil por clae-dad de dicksonia sellowiana (presl.). Hook, dicksoniaceae. Revista Brasileira de Plantas Medicinais, 18, 230-239. doi: 10.1590/1983-084X/15_106

Park, Y. W. (1996). Determination of moisture and ash contents of food. In: Nollet, L. M. L. Handbook of food analysis: physical characterization and nutrient analysis. New York: Marcel Dekker, 3, 59-92.

Rice-Evans, C. A. & Miller, N. J. & Paganga, G. (1996). Structure-antioxidant activity relationships of flavonoids and phenolic acids. Free Radical Biology and Medicine, Orland, 20 (7), 933-956. doi:10.1016/0891-5849(95)02227-9

Rodrigues, R. B. & Marx, F. (2006). Camu camu [Myrciaria dubia (HBK) Mc Vaugh]: a promising fruit from the Amazon Basin. NUTRITION-VIENNA, 30 (9), 376.

Rufino, M. D. S. M. et al. (2007). Metodologia científica: determinação da atividade antioxidante total em frutas pela captura do radical livre DPPH. Embrapa Agroindústria Tropical-Comunicado Técnico (INFOTECA-E).

Rufino, M. D. S. M., et al. (2010). "Bioactive compounds and antioxidant capacities of 18 nontraditional tropical fruits from Brazil." Food Chemistry 121(4), 996-1002. doi:10.1016/j.foodchem.2010.01.037

Sánchez ‐ Moreno, C. & Larrauri, J. A. & Saura-Calixto, F. (1998). Um procedimento para medir a eficiência anti-radicalar de polifenóis. Jornal da Ciência da Alimentação e Agricultura, 76 (2), 270-276. doi: 10.1002/(SICI)1097-0010(199802)76:2<270::AID-JSFA945>3.0.CO;2-9

Santos, I. L., et al. (2021). Camu-camu [Myrciaria dubia (HBK) McVaugh]: a review of properties and proposals of products for integral valorization of raw material. Food Chemistry, 131-290. doi: 10.1016/j.foodchem.2021.131290

Schimidt P. C. (1989). Phytopharmaceutical Technology. Boca Raton: CRC.

Sharapin, N. (2000). Fundamentos de Tecnologia de Produtos Fitoterápicos. Santa Fé de Bogotá-Colombia:CYTED, 145-157.

Tanaka, O., et al. (1996). Application of saponins in foods and cosmetics: Saponins of Mohave yucca and Sapindus mukurossi. Saponins used in Food and Agriculture, 1-11. doi: 10.1007/978-1-4613-0413-5_1

Teixeira, A. S. & Chaves, L. Da S. & Yuyama, K. (2004). Esterases no exame da estrutura populacional de Camu-camu (Myrciaria dubia (Kunth) McVaugh-Myrtaceae). Acta Amazonica, Manaus, 34 (1). doi: 10.1590/S0044-59672004000100011

Tiwari, P., et al. (2011). Phytochemical screening and Extraction: A Review. Internationale Pharmaceutica Sciencia, 1 (1), 98-106.

Solis V. S., et al. (2009). Evaluacion de la actividad antioxidante de la pulpa, cascara y semilla del fruto del camu camu (Myrciaria dubia H.B.K.). Revista Sociedad Química Perú. 75, 293-299.

Velioglu, Y. S. (1998). Antioxidant activity and total phenolic in selected fruits, vegetables, and grain products. J. Agric. Food Chem., 46, 4113-4117. doi: 10.1021/jf9801973

Villanueva-Tiburcio, J. E. & Condezo-Hoyos, L. A. & Asquieri, E. R. (2010). Anthocyanins, ascorbic acid, total polyphenols and antioxidant activity in camucamu (Myrciaria dubia (HBK) McVaugh) peels. Ciênc. Tecnol. Aliment. 30, 151–160. doi: 10.1590/s0101-20612010000500023

Who. (2011). Quality control methods for herbal materials. World Health Organization, Geneve. 173.

Willerding, A. L., et al. (2020). Estratégias para o desenvolvimento da bioeconomia no estado do Amazonas. Estud. av., São Paulo, 34 (98), 145-166. doi: 10.1590/s0103-4014.2020.3498.010

Yuyama, K. & Aguiar, J. P. L. & Yuyama, L. K. O. (2002). Camu-camu: uma fruta fantástica como fonte de vitamina C1. Acta Amazonica, 32, 169-174. doi: 10.1590/1809-43922002321174

Zanatta, C. F. & Mercadante, A. (2007). Composição da fruta tropical brasileira camu-camu (Myrciaria dubia). Food Chemistry, 101 (4), 1526-1532. doi:10.1016/j.foodchem.2006.04.004

Zapata, S. M. & Dufour, J. P. (1993). "Camu-camu Myrciaria dubia (HBK) McVaugh: Chemical composition of fruit." Journal of the Science of Food and Agriculture, 61(3), 349-351. doi: 10.1002/jsfa.2740610310

Zeng, M., et al. (2007). Microscopic examination of changes of plant cell structure in corn stover due to hot water pretreatment and enzymatic hydrolysis. Biotechnology and Bioengineering, 97, 265- 278. doi: 10.1002/bit.21298

Published

18/01/2022

How to Cite

SILVA, C. S. M. da .; MOURÃO, R. H. V. . Antioxidant activity of Myrciaria dubia (camu-camu) extracts Myrtaceae. Research, Society and Development, [S. l.], v. 11, n. 2, p. e5811225130, 2022. DOI: 10.33448/rsd-v11i2.25130. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/25130. Acesso em: 22 dec. 2024.

Issue

Section

Health Sciences