Antagonismo de diferentes espécies de Lactobacillus spp. frente a fitobactérias

Authors

DOI:

https://doi.org/10.33448/rsd-v11i2.25760

Keywords:

Bioprospecting; Biological control; Lactobacillus spp; Solanum lycopersicum; Xanthomonas spp.

Abstract

Tomato (Solanum lycopersicum L.) is one of the most important and most cultivated vegetables in the world, having a market for derivative products with high commercial appeal. The inoculation of probiotic bacteria can act in the prevention of diseases such as bacterial spots, bacterial wilt, soft rot, among others, in addition to reducing the use of pesticides and antibiotics, helping to mitigate the impact on humans and environmental health. Part of the samples used for the isolation of LAB was collected from leaves of healthy tomato plants at the Escola de Agronomia (EA) of the Federal University of Goiás (UFG) and another part was obtained from a starter culture from the local market. Two methods of antagonism were used, the diffusion test in a liquid medium and the test in a solid medium. Of the 13 strains tested in this study, 10 showed activity against different phytobacteria, the antagonistic activity test in liquid medium was better compared to a solid medium, broad-spectrum antimicrobial action, and an inhibition halo of up to 22 mm of diameter from a strain obtained from a starter culture. Thus, this work demonstrates the antimicrobial activity of LAB strains tested in vitro against different phytobacteria.

Author Biographies

Rodolfo Sodré Tavares, Federal University of Goiás

Biomedical, Master by the Postgraduate Program in Biology of the Parasite-Host Relationship (PPGBRPH), by the Institute of Tropical Pathology and Public Health (IPTSP) of the Federal University of Goiás (UFG).

Paulo Alex Neves da Silva, Federal University of Goiás

Graduated in Biological Sciences from Centro Universitário São Lucas (2012), specialization in clinical microbiology (2016), specialization in sanitation and environmental health (2020), master's degree in host parasite biology (immunology) (2017). Doctoral candidate in Health Sciences (microbiology) at FM/UFG. He is currently a clinical pathologist in an urgent/emergency laboratory and a university professor, teaching courses in the health area. He has experience in the field of Immunology, working mainly on humoral immunity against Zika virus infection; clinical and environmental microbiology, working mainly in the department of biotechnology, bacteriology and virology; hematology, hormones, biochemistry and urinalysis. He is currently developing research in the field of virology: Research on arboviruses (ChikV, DenV and Zikv) of medical interest and on the novel coronavirus, which causes COVID-19. Member of the Microorganism Biotechnology Laboratory (LBMIC).

Ludmila Batista Machado, Catholic University of Goiás

He completed his graduation in the Nutrition course at the Pontifical Catholic University of Goiás (PUC-GO) as a full scholarship holder from ProUni. She participated in the Nutriguia extension project at the Pontifical Catholic University of Goiás (PUC-GO). She was a fellow of the extension project of the Collaborating Center for School Food and Nutrition (CECANE-UFG). She participated in the scientific initiation project as a volunteer at PUC-GO. She is currently a resident of the Multiprofessional Residency Program in Urgency and Trauma at the State Emergency Hospital of Goiânia Dr. Valdemiro Cruz-HUGO, by the State Health Department (SES / GO).

Abadia dos Reis Nascimento, Federal University of Goiás

Graduated in Agronomic Engineering from the Escola Superior de Ciências Agrárias de Rio Verde (2002). Master's and Doctorate in Plant Production from the Federal University of Goiás (UFG). He held a Post-Doctorate at Embrapa Vegetables. She has been a professor at the Federal University of Goiás since 2011 and is currently Associate Professor II. She is a permanent professor at the Graduate Programs in Agronomy and Agribusiness at UFG. She was the coordinator of the first Specialization Course in Tomatoes for Industrial Processing (UFG). She is currently vice-director of the UFG's Caldas Novas Campus. She teaches the disciplines of Production of Vegetables and Urban Gardens: PANCs, aromatic and medicinal for the Graduation and the Disciplines of Vegetable Production, Production of Vegetable Seedlings and Production Chains of the State of Goiás for Post-Graduation. She develops research in the area of ​​phytotechnics, mainly in crops: tomato, lettuce, watermelon, sweet corn, pepper and production of vegetable seedlings. Delegate of the Brazilian Horticulture Association (ABH) of the State of Goiás.

Lilian Carla Carneiro, Federal University of Goiás

Graduation in Biological Sciences Medical-biomedicine modality from the Pontifical Catholic University of Goiás (2000), master's degree in Biochemistry and Molecular Biology from the Federal University of Goiás (2004), PhD in Cellular and Molecular Biology from the Federal University of Goiás (2010), postgraduate PhD in Molecular and Imaging Diagnosis of Infectious and Parasitic Diseases, from UFCSPA (2013). Professor at PUC-GO (2002), professor at the State University of Goiás (2003 to 2012), researcher at the Institute of Molecular Biology of Paraná (2013 to 2015). I am currently a professor at the Federal University of Goiás. I have experience in the field of microbiology, with emphasis on applications of molecular biology, working mainly on the following topics: microbiological analysis, microorganism biotechnology and biotechnology applied to public health.

José Daniel Gonçalves Vieira, Federal University of Goiás

He holds a degree in Pharmacy-Biochemistry from the Federal University of Ouro Preto (1986), a master's degree in Agricultural Microbiology from the Federal University of Viçosa (1990) and a PhD in Biological Sciences (Microbiology) from the University of São Paulo (1999). He is currently Associate Professor IV at the Federal University of Goiás. He has experience in the field of Microbiology, with an emphasis on Environmental Microbiology and microorganism ecology working in the bioremediation process, microbial diversity in different Brazilian biomes. Isolation of actinomycetes and determination of their antimicrobial and antitumor activity and enzyme production. Production of bioethanol, biodiesel, butanol and propanol, biotechnological use of co-products of the biodiesel industry production of biosurfactants and bioflocculants. Endophytic microorganisms.

References

Biagi, C. M. R., & Azevedo, J. L. (1992). Detecção de bacteriocinas produzidas por bactérias fitopatogênicas dos gêneros Erwinia, Pseudomonas e Xanthomonas. Scientia Agricola, 49, 1-8.

Çadirci, B. H., & Çitak, S. (2005). A Comparison of Two Methods Used for Measuring Antagonistic Activity of Lactic Acid Bacteria. Pakistan Journal of Nutrition, 4, 237-241.

Carr, J. F., Chill, D., & Maida, N. (2002). The Lactic Acid Bacteria: A Literature Survey, Critical Reviews in Microbiology, 28, 281-370.

Costa, C. E., Teixeira, S. F. M., Dantas, M. V. T., Melo, P. S. V., Araújo, C. A. S., & Rolim, N. B. (2009). Microbiological samples storage and preservation principles, Ciência Animal, 19, 111-112.

Daranas, N., Badosa, E., Francés, J., Montesinos, E., & Bonaterra, A. (2018). Enhancing water stress tolerance improves fitness in biological control strains of Lactobacillus plantarum in plant environments. PLoS ONE, 13, 1-21.

Fang, X., Duan, Q., Wang, Z., Li, F., Du, J., Ke, W., Liu, D., Beier, R. C., Guo, X., & Zhang, Y. (2020). Products of Lactobacillus delbrueckii subsp. bulgaricus Strain F17 and Leuconostoc lactis Strain H52 Are Biopreservatives for Improving Postharvest Quality of ‘Red Globe’ Grapes, Microorganisms, 8, 1-16.

FAO-FAOSTAT (2021). Food and Agriculture Organization of the United Nations. Retrieved Jun 4, 2021, from http://faostat.fao.org/.

Forhad, M. H., Rahman, S. M. K., Rahman, S., Saikot, F. K., & Biswas, K. C. (2015). Probiotic Properties Analysis of Isolated Lactic Acid Bacteria from Buffalo Milk, Archives of Clinical Microbiology, 7, 1-6.

Gänzle, M. G., Höltzele, A., Walter, J., Jung, G., & Hammes, W. P. (2000). Characterization of Reutericyclin Produced by Lactobacillus reuteri LTH2584. Applied and Environmental Microbiology, 66, 4325-4333.

Gordon, S. (2008). Elie Metchnikoff: Father of natural immunity. European Journal of Immunology, 38, 3257-3264.

IBGE (2021). Sistema Brasileiro de Geografia e Estatística. Retrieved Jun 4, 2021, from https://sidra.ibge.gov.br/tabela/1612.

Itako, A. T., Júnior, J. B. T., Júnior, T. A. F. S., Soman, J. M., & Maringoni, A. C. (2015). Chemical products induce resistance to Xanthomonas perforans in tomato. Brazilian Journal of Microbiology, 46, 701-706.

Khanna, K., Sharma, A., Ohri, P., Bhardwaj, R., Allah, E. F. A., Hashem, A., & Ahmad, P. (2019). Impact of plant growth promoting rhizobacteria in the orchestration of Lycopersicon esculentum mill. Resistance to plant parasitic nematodes: A metabolomic approach to evaluate defense responses under field conditions. Biomolecules, 9, 4-30.

Limanska, N., Korotaeva, N., Biscola, V., Ivanystsia, T., Merlich, A., Franco, B. D. G. M., Chobert, J. M., Ivanytsia, V., & Haertlé, T. (2015). Study of the Potential Application of Lactic Acid Bacteria in the Control of Infection Caused by Agrobacterium tumefaciens. Journal of Plant Pathology & Microbiology, 06, 1-13.

Midha, S., & Patil, P. B. (2014). Genomic Insights into the Evolutionary Origin of Xanthomonas axonopodispv. citri and Its Ecological Relatives. Appliedand Environmental Microbiology, 80, 6266-6279.

Modesto, G. L. A., Teixeira, S. A., Silveira, S. R. M., & Almeida, T. M. M. (2016). Normas e Procedimentos de Segurança e Boas Práticas dos Laboratórios da Embrapa Agroindústria Tropical, Embrapa Agroindústria Tropical, 1-55.

Mohamed, A. H., Osman, G. Y., Zowail, M. E. M., & Esawy, H. M. I. E. (2016). Effect of Lactobacillus sporogenes (probiotic) on certain parasitological and molecular aspects in Schistosoma mansoni infected mice. Journal of Parasitic Diseases, 40, 823-832.

Pandey, N., Malik, R. K., Kaushik, J. K., & Singroha, G. (2013). Gassericin A: a circular bacteriocin produced by Lactic acid bacteria Lactobacillus gasseri. World Journal of Microbiology and Biotechnology, 29, 1977-1987.

Peixoto., R. A., Mariano, R. L. R., & Viana, O. I. (2006). Meio semi-seletivo para isolamento de Xanthomonas campestris pv. Viticola, Ciência Rural, 36, 1317-1320.

Prabhurajeshwar, C., & Chandrakanth, K. R. (2017). Probiotic potential of Lactobacilli with antagonistic activity against pathogenic strains: An in vitro validation for the production of inhibitory substances, Biomedical Journal, 40, 270-283.

Rodrigues, A. A. (2009). Atividade antimicrobiana e produção de enzimas de interesse biotecnológico de bactérias isoladas de diferentes habitats. Dissertação (área de concentração em microbiologia) - Faculdade de Farmácia da Universidade Federal de Goiás, 2009.

Silva., A. G. D., Rocha, C. M., Carvalho, O. A., Fernandes, A. C. M., & Carmo, F. G. M. (2006). Efeito de produtos químicos e biológicos sobre a mancha bacteriana, flora microbiana no filoplano e produtividade de pimentão. Horticultura Brasileira, 24, 1-7.

Teper, D., Girija, A. M., Bosis, E., Popov, G., Savidor, A., & Sessa, G. (2018). The Xanthomonas euvesicatoria type III effector XopAU is a nactive protein kinase that manipulates plant MAP kinase signaling. PLoS pathogens, 14, 1-33.

Wang, Y., Sun, P., Li, H., Adhikari, B, P., & Li, D. (2018). Rheological Behavior of Tomato Fiber Suspensions Produced by High Shear and High Pressure Homogenization and Their Application in Tomato Products. International Journal of Analytical Chemistry, 18, 1-12.

Zaluga, J., Stragier, P., Vaerenbergh, V. J., Maes, M., & De vos, P. (2013). Multilocus variable-number-tandem-repeats analysis (MLVA) distinguishes a clonal complex of Clavibacter michiganensis subsp. michiganensis strains isolated from recent outbreaks of bacterial wilt and canker in Belgium. BMC microbiology, 13, 1-15.

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Published

25/01/2022

How to Cite

TAVARES, R. S. .; SILVA, P. A. N. da .; MACHADO, L. B. .; NASCIMENTO, A. dos R. .; CARNEIRO, L. C. .; VIEIRA, J. D. G. . Antagonismo de diferentes espécies de Lactobacillus spp. frente a fitobactérias. Research, Society and Development, [S. l.], v. 11, n. 2, p. e28011225760, 2022. DOI: 10.33448/rsd-v11i2.25760. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/25760. Acesso em: 5 nov. 2024.

Issue

Vol. 11 No. 2

Section

Agrarian and Biological Sciences

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Copyright (c) 2022 Rodolfo Sodré Tavares; Paulo Alex Neves da Silva; Ludmila Batista Machado; Abadia dos Reis Nascimento; Lilian Carla Carneiro; José Daniel Gonçalves Vieira

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