α-Glucosidase inhibitory properties of leaves and bark extracts of Curatella americana L.

Authors

DOI:

https://doi.org/10.33448/rsd-v11i4.27052

Keywords:

Tannins; Postprandial hyperglycemia; Antioxidant activity; Diabetes.

Abstract

In this article, we investigate the inhibitory activity of methanolic extracts of bark and leaves of Curatella americana L. against α-glucosidase. Furthermore, antioxidant activity was evaluated using DPPH and ABTS assays, and the interaction of the identified compound of the extracts and α-glucosidase was tested by molecular docking. The results show that C. americana has a strong inhibitory activity against α-glucosidase reaching an IC 50 value of 7.29 µg/ml and 7.26 µg/ml for the extracts of bark and leaves, respectively. The enzyme kinetics reveals a mixed competitive mechanism for the leaves extract and an uncompetitive type of inhibition for the bark extract. The results of the antioxidant activity showed that both extracts have great antioxidant capacity, with the leaves extract having a better performance. The identified compounds of the extracts have a high binding affinity to the enzyme. Therefore, the study showed that C. americana extracts have a great potential for the treatment of diabetes, possibly serving as a therapeutic option to treat postprandial hyperglycemia and prevent long-term pathologies associated with diabetes.

Author Biography

Gabriela Eustáquio Lacerda, Federal University of Tocantins

  •  

References

Ahmed, O. M., Hassan, M. A., Abdel-Twab, S. M., & Abdel Azeem, M. N. (2017). Navel orange peel hydroethanolic extract, naringin and naringenin have anti-diabetic potentials in type 2 diabetic rats. Biomedicine and Pharmacotherapy, 94, 197–205. https://doi.org/10.1016/j.biopha.2017.07.094

Barrett, A. H., Farhadi, N. F., & Smith, T. J. (2018). Slowing starch digestion and inhibiting digestive enzyme activity using plant flavanols/tannins— A review of efficacy and mechanisms. LWT, 87, 394–399. https://doi.org/10.1016/j.lwt.2017.09.002

Beidokhti, M. N., & Jäger, A. K. (2017). Review of antidiabetic fruits, vegetables, beverages, oils and spices commonly consumed in the diet. In Journal of Ethnopharmacology (Vol. 201, pp. 26–41). Elsevier Ireland Ltd. https://doi.org/10.1016/j.jep.2017.02.031

Cecílio, A. B., Faria, D. B. de, Oliveira, P. D. C., Caldas, S., Oliveira, D. A. de, Sobral, M. E. G., Duarte, M. G. R., Moreira, C. P. D. S., Silva, C. G., & Almeida, V. L. de. (2012). Screening of Brazilian medicinal plants for antiviral activity against rotavirus. Journal of Ethnopharmacology, 141(3), 975–981. https://doi.org/10.1016/j.jep.2012.03.031

Costa, O. J. da, Barbosa, R. dos S., Soares, I. M., Souza E. E. de, Gellen, L. F. A., Lemos, J. P. P. P., Aguiar, R. W. S., Montel, A. L. B., & Ascencio, S. D. (2020). Inhibitory effects of Anadenanthera colubrina (Vell.) Brenan stem bark extract on -glucosidase activity and oxidative stress. Journal of Medicinal Plants Research, 14(11), 583–592. https://doi.org/10.5897/JMPR2020.7025

Deseo, M. A., Elkins, A., Rochfort, S., & Kitchen, B. (2020). Antioxidant activity and polyphenol composition of sugarcane molasses extract. Food Chemistry, 314. https://doi.org/10.1016/j.foodchem.2020.126180

Fu, M., Shen, W., Gao, W., Namujia, L., Yang, X., Cao, J., & Sun, L. (2021). Essential moieties of myricetins, quercetins and catechins for binding and inhibitory activity against α-Glucosidase. Bioorganic Chemistry, 115. https://doi.org/10.1016/j.bioorg.2021.105235

Ghani, U. (2015). Re-exploring promising α-glucosidase inhibitors for potential development into oral anti-diabetic drugs: Finding needle in the haystack. In European Journal of Medicinal Chemistry (Vol. 103, pp. 133–162). Elsevier Masson SAS. https://doi.org/10.1016/j.ejmech.2015.08.043

Habib, H. M., Platat, C., Meudec, E., Cheynier, V., & Ibrahim, W. H. (2014). Polyphenolic compounds in date fruit seed (Phoenix dactylifera): Characterisation and quantification by using UPLC-DAD-ESI-MS. Journal of the Science of Food and Agriculture, 94(6), 1084–1089. https://doi.org/10.1002/jsfa.6387

Hamed, Y. S., Abdin, M., Rayan, A. M., Saleem Akhtar, H. M., & Zeng, X. (2021). Synergistic inhibition of isolated flavonoids from Moringa oleifera leaf on α-glucosidase activity. LWT, 141. https://doi.org/10.1016/j.lwt.2021.111081

Henriques, S. V. C., & Almeida, S. S. M. da S. de. (2015). Identificação do caráter medicinal da espécie Curatella americana por meio das folhas. Estação Científica (UNIFAP), 3(2), 89–97.

Junejo, J. A., Rudrapal, M., Nainwal, L. M., & Zaman, K. (2017). Antidiabetic activity of hydro-alcoholic stem bark extract of Callicarpa arborea Roxb. with antioxidant potential in diabetic rats. Biomedicine and Pharmacotherapy, 95, 84–94. https://doi.org/10.1016/j.biopha.2017.08.032

Kazeem, M. I., Akanji, M. A., Hafizur, R. M., & Choudhary, M. I. (2012). Antiglycation, antioxidant and toxicological potential of polyphenol extracts of alligator pepper, ginger and nutmeg from Nigeria. Asian Pacific Journal of Tropical Biomedicine, 2(9), 727–732. https://doi.org/10.1016/S2221-1691(12)60218-4

Kumari, M., & Jain, S. (2012). Tannin: An Antinutrient with Positive Effect to Manage Diabetes. www.isca.in

Ky, I., & Teissedre, P. L. (2015). Characterisation of Mediterranean grape pomace seed and skin extracts: Polyphenolic content and antioxidant activity. Molecules, 20(2), 2190–2207. https://doi.org/10.3390/molecules20022190

Laddha, A. P., & Kulkarni, Y. A. (2019). Tannins and vascular complications of Diabetes: An update. In Phytomedicine (Vol. 56, pp. 229–245). Elsevier GmbH. https://doi.org/10.1016/j.phymed.2018.10.026

Lee, D. Y., Kim, H. W., Yang, H., & Sung, S. H. (2017). Hydrolyzable tannins from the fruits of Terminalia chebula Retz and their α-glucosidase inhibitory activities. Phytochemistry, 137, 109–116. https://doi.org/10.1016/j.phytochem.2017.02.006

Lopes, R. H. O., Macorini, L. F. B., Antunes, K. Á., Espindola, P. P. D. T., Alfredo, T. M., Rocha, P. D. S. da, Pereira, Z. V., Santos, E. L. dos, & Souza, K. D. P. (2016). Antioxidant and Hypolipidemic Activity of the Hydroethanolic Extract of Curatella americana L. Leaves. Oxidative Medicine and Cellular Longevity, 2016. https://doi.org/10.1155/2016/9681425

Ma, W., Waffo-Téguo, P., Alessandra Paissoni, M., Jourdes, M., & Teissedre, P. L. (2018). New insight into the unresolved HPLC broad peak of Cabernet Sauvignon grape seed polymeric tannins by combining CPC and Q-ToF approaches. Food Chemistry, 249, 168–175. https://doi.org/10.1016/j.foodchem.2018.01.005

Oboh, G., Ogunsuyi, O. B., Ogunbadejo, M. D., & Adefegha, S. A. (2016). Influence of gallic acid on α-amylase and α-glucosidase inhibitory properties of acarbose. Journal of Food and Drug Analysis, 24(3), 627–634. https://doi.org/10.1016/j.jfda.2016.03.003

Oestreich Filho, E. (2014). Fitossociologia, diversidade e similaridade entre fragmentos de cerrado stricto sensu sobre neossolos quartzarênicos órticos, nos municípios de Cuiabá e Chapada dos Guimarães, estado de Mato Grosso, Brasil.

Ou-Yang, C., Chai, W., Xu, X., Song, S., Wei, Q., Huang, Q., & Zou, Z. (2020). Inhibitory potential of proanthocyanidins from the fruit pulp of Clausena lansium (Lour.) Skeels against α-glucosidase and non-enzymatic glycation: Activity and mechanism. Process Biochemistry, 91, 364–373. https://doi.org/10.1016/j.procbio.2020.01.006

Park, S. R., Kim, J. H., Jang, H. D., Yang, S. Y., & Kim, Y. H. (2018). Inhibitory activity of minor phlorotannins from Ecklonia cava on α-glucosidase. Food Chemistry, 257, 128–134. https://doi.org/10.1016/j.foodchem.2018.03.013

Peixoto Sobrinho, T. J. da S., Silva, C. H. T. P. da, Nascimento, J. E. do, Monteiro, J. M., Albuquerque, U. P. de, & Amorim, E. L. C. de. (2008). Validação de metodologia espectrofotométrica para quantificação dos flavonóides de Bauhinia cheilantha (Bongard) Steudel. Revista Brasileira de Ciências Farmacêuticas, 44, 683–689.

Proença, C., Freitas, M., Ribeiro, D., Oliveira, E. F. T., Sousa, J. L. C., Tomé, S. M., Ramos, M. J., Silva, A. M. S., Fernandes, P. A., & Fernandes, E. (2017). α-Glucosidase inhibition by flavonoids: an in vitro and in silico structure–activity relationship study. Journal of Enzyme Inhibition and Medicinal Chemistry, 32(1), 1216–1228. https://doi.org/10.1080/14756366.2017.1368503

Ramkissoon, J. S., Mahomoodally, M. F., Subratty, A. H., & Ahmed, N. (2016). Inhibition of glucose- and fructose-mediated protein glycation by infusions and ethanolic extracts of ten culinary herbs and spices. Asian Pacific Journal of Tropical Biomedicine, 6(6), 492–500. https://doi.org/10.1016/j.apjtb.2016.01.016

Ramu, R., Shirahatti, P. S., Zameer, F., Ranganatha, L. v., & Nagendra Prasad, M. N. (2014). Inhibitory effect of banana (Musa sp. var. Nanjangud rasa bale) flower extract and its constituents Umbelliferone and Lupeol on α-glucosidase, aldose reductase and glycation at multiple stages. South African Journal of Botany, 95, 54–63. https://doi.org/10.1016/j.sajb.2014.08.001

Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., & Rice-Evans, C. (1999). Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine, 26(9–10), 1231–1237. https://doi.org/10.1016/S0891-5849(98)00315-3

Serrano, J., Puupponen-Pimiä, R., Dauer, A., Aura, A. M., & Saura-Calixto, F. (2009). Tannins: Current knowledge of food sources, intake, bioavailability and biological effects. In Molecular Nutrition and Food Research. 53(2), S310–S329. Wiley-VCH Verlag. https://doi.org/10.1002/mnfr.200900039

Soares, I. M., Bastos, E. G. P., Sobrinho, T. J. S. P., Alvim, T. C., Silveira, M. A., Aguiar, R. W. S., & Ascêncio, S. D. (2014). Conteúdo fenólico e atividade antioxidante de diferentes cultivares de ipomoea batatas (l.) lam. obtidas por melhoramento genético para produção industrial de etanol. Revista de Ciências Farmacêuticas Básica e Aplicada, 35(3).

Sobrinho, T. J. S. P., Castro, V. T. N. A., Saraiva, A. M., Almeida, D. M., Tavares, E. A., & Amorim, E. L. C. (2011). Phenolic content and antioxidant capacity of four cnidoscolus species (euphorbiaceae) used as ethnopharmacologicals in caatinga, brazil. African Journal of Pharmacy and Pharmacology, 5(20), 2310–2316. https://doi.org/10.5897/AJPP11.608

Teles Fujishima, M., Silva, N., Ramos, R., Batista Ferreira, E., Santos, K., Silva, C., Silva, J., Campos Rosa, J., & Santos, C. (2018). An Antioxidant Potential, Quantum-Chemical and Molecular Docking Study of the Major Chemical Constituents Present in the Leaves of Curatella americana Linn. Pharmaceuticals, 11(3), 72. https://doi.org/10.3390/ph11030072

Downloads

Published

10/03/2022

How to Cite

BARBOSA, R. dos S. .; SOARES, I. M.; LACERDA, G. E. .; MATUMOTO, F. H. .; RECH, T. R.; SOUZA, A. S. de; ALVIM, T. da C. .; AGUIAR, R. W. de S.; ASCENCIO, S. D. . α-Glucosidase inhibitory properties of leaves and bark extracts of Curatella americana L. . Research, Society and Development, [S. l.], v. 11, n. 4, p. e3811427052, 2022. DOI: 10.33448/rsd-v11i4.27052. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/27052. Acesso em: 4 nov. 2024.

Issue

Section

Agrarian and Biological Sciences