Antibacterial activity of Lippia sidoides Cham against periodontopathogens: an in vitro study
Keywords:Chlorhexidine; Periodontics; Periodontal diseases.
The aim of this study was to evaluate the antimicrobial susceptibility of essential oil from Lippia sidoides Cham, against Aggregatibacter actinomycetecomitans ATCC 29522 and Porphyromonas gingivalis ATCC 0644. The antimicrobial activity of L.sidoides Cham oil extracted from leaves and flowers and the constituents thymol and carvacrol through Minimum Inhibitory Concentration (MIC) and minimal bactericidal determination (CBM) against periodontopathogens Aggregatibacter actinomycetemcomitans (ATCC), Porphyromonas gingivalis (ATCC ). Te results revealed that the MIC recorded was 44.23 to 39.3 mg/mL for thymol and 28.45 to 28.15 mg/mL for carvacrol. These concentrations were considered bactericidal when CBM was evaluated. All analyzed substances showed a synergistic effect in association with chlorhexidine. Thus, it can be concluded that Lippia sidoides Cham was effective in inhibiting the in vitro growth of periodontopathogenic bacteria, presenting, therefore, biotechnological potential for use in the field of dentistry.
Alexa, E., Sumalan, R. M., Danciu, C., Obistioiu, D., Negrea, M., Poiana, M. A., et al. (2018) Synergistic Antifungal, Allelopatic and Anti-Proliferative Potential of Salvia officinalis L., and Thymus vulgaris L. Essential Oils. Molecules. 16;23 (1).
Alves, F. C. B., Barbosa, L. N., Andrade, B., Albano, M., Furtado, F. B., Marques Pereira, A. F., et al.(2016). Short communication: Inhibitory activities of the lantibiotic nisin combined with phenolic compounds against Staphylococcus aureus and Listeria monocytogenes in cow milk. Journal of dairy science. 99 (3):1831-6.
Araujo, M. J. C., Camara, C., Moraes, M. M., & Born, F. S. (2020) Insecticidal properties and chemical composition of Piper aduncum L., Lippia sidoides Cham. and Schinus terebinthifolius Raddi essential oils against Plutella xylostella L. Anais da Academia Brasileira de Ciencias. 2020;92 (suppl 1):e20180895.
Aryal, B., Niraula, P., Khadayat, K., Adhikari, B., Khatri Chhetri, D., Sapkota, B. K., et al. (2021) Antidiabetic, Antimicrobial, and Molecular Profiling of Selected Medicinal Plants. Evidence-based complementary and alternative medicine : eCAM2021:5510099.
Barbosa, R., Cruz-Mendes, Y., Silva-Alves, K. S., Ferreira-da-Silva, F. W., Ribeiro, N. M., Morais, L. P., et al. (2017) Effects of Lippia sidoides essential oil, thymol, p-cymene, myrcene and caryophyllene on rat sciatic nerve excitability. Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas. 19;50 (12):e6351.
Batista, E. S., Franmir, R. B., Majolo, C., & Kioshi, L. A. K. Lippia alba essential oil as anesthetic for tambaqui. Aquaculture. 495:545-9.
Ben Arfa, A., Combes, S., Preziosi-Belloy, L., Gontard, N., & Chalier, P. (2006) Antimicrobial activity of carvacrol related to its chemical structure. Letters in applied microbiology. 43 (2):149-54.
Botelho, M. A., Nogueira, N. A., Bastos, G. M., Fonseca, S. G., Lemos, T. L., Matos, F. J., et al. (2007) Antimicrobial activity of the essential oil from Lippia sidoides, carvacrol and thymol against oral pathogens. Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas. 40 (3):349-56.
Carvalho, I. T., Estevinho, B. N., & Santos, L. (2016) Application of microencapsulated essential oils in cosmetic and personal healthcare products - a review. International journal of cosmetic science. 38 (2):109-19.
Couto De Oliveira, G., Ferraz, C. S., Andrade Junior, C. V., & Pithon, M. M. (2013) Chlorhexidine gel associated with papain in pulp tissue dissolution. Restorative dentistry & endodontics. 38 (4):210-4.
Dorman, H. J., & Deans, S. G. (2000) Antimicrobial agents from plants: antibacterial activity of plant volatile oils. Journal of applied microbiology. 88 (2):308-16.
Figueredo, C. M., Lira-Junior, R., & Love, R. M.(2019) T and B Cells in Periodontal Disease: New Functions in A Complex Scenario. International journal of molecular sciences. 14;20 (16).
Freires, I. A., Santaella, G. M., de Cassia Orlandi Sardi, J., & Rosalen, P. L.(2018) The alveolar bone protective effects of natural products: A systematic review. Archives of oral biology. 87:196-203.
Ginting, E. V., Retnaningrum, E., & Widiasih, D. A.(2021) Antibacterial activity of clove (Syzygium aromaticum) and cinnamon (Cinnamomum burmannii) essential oil against extended-spectrum beta-lactamase-producing bacteria. Veterinary world. 14 (8):2206-11.
Khruengsai, S., Sripahco, T., Rujanapun, N., Charoensup, R., & Pripdeevech, P.(2021) Chemical composition and biological activity of Peucedanum dhana A. Ham essential oil. Scientific reports. 27;11 (1):19079.
Maragoni, S. T., Moraes, S. H., Utrera, L. A., & Casemiro, M. G. M. (2018) Diterpenes of the pimarane type isolated from Viguiera arenaria: promising in vitro biological potential as therapeutic agents for endodontics. Journal of Pharmacognosy and Phytotherapy. 10 (2):34-44.
Melo, J. O., Fachin, A. L., Rizo, W. F., Jesus, H. C., Arrigoni-Blank, M. F., Alves, P. B., et al. (2014) Cytotoxic effects of essential oils from three Lippia gracilis Schauer genotypes on HeLa, B16, and MCF-7 cells and normal human fibroblasts. Genetics and molecular research : GMR 8;13 (2):2691-7.
Mendes, F. S. F., Garcia, L. M., Moraes, T. D. S., Casemiro, L. A., Alcantara, C. B., Ambrosio, S. R., et al.(2020) Antibacterial activity of salvia officinalis L. against periodontopathogens: An in vitro study. Anaerobe. 63:102194.
Morão, R. P., Almeida, A. C., Martins, E. R., & Prates, J. P.(2016) Constituintes Quimicos e Principios Farmacológicos do óleo Essencial de Alecrim Pimenta (Lippia Origanoides). Revista Unimontes Ciientífica. 8 (1):1-8.
Moreti, D. L. C., Leandro, L. F., da Silva Moraes, T., Moreira, M. R., Sola Veneziani, R. C., Ambrosio, S. R., et al.(2017) Mikania glomerata Sprengel extract and its major compound ent-kaurenoic acid display activity against bacteria present in endodontic infections. Anaerobe47:201-8.
Nagoor Meeran, M. F., Javed, H., Al Taee, H., Azimullah, S., & Ojha, S. K. (2017) Pharmacological Properties and Molecular Mechanisms of Thymol: Prospects for Its Therapeutic Potential and Pharmaceutical Development. Frontiers in pharmacology. 8:380.
Nazzaro, F., Fratianni, F., De Martino, L., Coppola, R., & De Feo, V. (2013) Effect of essential oils on pathogenic bacteria. Pharmaceuticals. 25;6 (12):1451-74.
Nhu-Trang, T. T., Casabianca, H., & Grenier-Loustalot, M. F. (2006) Deuterium/hydrogen ratio analysis of thymol, carvacrol, gamma-terpinene and p-cymene in thyme, savory and oregano essential oils by gas chromatography-pyrolysis-isotope ratio mass spectrometry. Journal of chromatography A. 3;1132 (1-2):219-27.
Olmedo-Juarez, A., Briones-Robles, T. I., Zaragoza-Bastida, A., Zamilpa, A., Ojeda-Ramirez, D., Mendoza de Gives, P., et al.(2019) Antibacterial activity of compounds isolated from Caesalpinia coriaria (Jacq) Willd against important bacteria in public health. Microbial pathogenesis. 136:103660.
Pithon, M. M., Santana, D. A., Sousa, K. H., & Farias, I. M. (2013) Does chlorhexidine in different formulations interfere with the force of orthodontic elastics? The Angle orthodontist. 83 (2):313-8.
Rosato, A., Vitali, C., Laurentis, N., Armenise, D., & Milillo, M. A. (2007) Antibacterial Effect of Some Essential Oils Administered Alone Or In Combination With Norfloxacin. Phytomedicine : International journal of phytotherapy and phytopharmacology. 14:727-32.
Sampaio, G. M., de Meneses, I. H., de Carvalho, F. G., Carlo, H. L., Munchow, E. A., Barbosa, T. S., et al.(2020) Antimicrobial, mechanical and biocompatibility analysis of chlorhexidine digluconate-modified cements. Journal of clinical and experimental dentistry. 12 (2):e178-e86.
Smith, F. L., & Baumgarth, N.(2019) B-1 cell responses to infections. Current opinion in immunology. 57:23-31.
Wijesundara, N. M., & Rupasinghe, H. P. V.(2019) Bactericidal and Anti-Biofilm Activity of Ethanol Extracts Derived from Selected Medicinal Plants against Streptococcus pyogenes. Molecules. 24;24 (6).
Woo, H. G., Chang, Y. K., Lee, J. S., & Song, T. J.(2021) Association of Periodontal Disease with the Occurrence of Unruptured Cerebral Aneurysm among Adults in Korea: A Nationwide Population-Based Cohort Study. Medicina. 30;57 (9).
Xu, J., Wu, C., Yang, Z., Liu, W., Chen, H., Batool, K., et al.(2020) For: Pesticide biochemistry and physiology recG is involved with the resistance of Bt to UV. Pesticide biochemistry and physiology. 167:104599.
How to Cite
Copyright (c) 2022 Isabel Celeste Caires Pereira Gusmão; Matheus Melo Pithon; Alessandra Estevam dos Santos ; Tainá Souza Silva ; Fabio Correia Sampaio
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
1) Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
2) Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
3) Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.