Actividad antibacteriana de Lippia sidoides Cham frente a periodontopatógenos: estudio in vitro

Autores/as

DOI:

https://doi.org/10.33448/rsd-v11i7.27141

Palabras clave:

Clorhexidina; Periodoncia; Enfermedades periodontales.

Resumen

El objetivo de este estudio fue evaluar la susceptibilidad antimicrobiana del aceite esencial de Lippia sidoides Cham frente a Aggregatibacter actinomycetecomitans ATCC 29522 y Porphyromonas gingivalis ATCC 0644. La actividad antimicrobiana del aceite de L.sidoides Cham extraído de hojas y flores y los constituyentes timol y carvacrol a través de la Concentración mínima inhibitoria (MIC) y determinación bactericida mínima (MBC) frente a los periodontopatógenos Aggregatibacter actinomycetemcomitans (ATCC), Porphyromonas gingivalis (ATCC). Resultados: revelaron que la CIM registrada fue de 44,23 a 39,3 mg/mL para timol y de 28,45 a 28,15 mg/mL para carvacrol. Estas concentraciones se consideraron bactericidas cuando se evaluó el CBM. Todas las sustancias analizadas mostraron un efecto sinérgico en asociación con la clorhexidina. Por lo tanto, se puede concluir que Lippia sidoides Cham fue eficaz para inhibir el crecimiento in vitro de bacterias periodontopatógenas, presentando, por tanto, potencial biotecnológico para su uso en odontología.

Citas

Alexa, E., Sumalan, R. M., Danciu, C., Obistioiu, D., Negrea, M., Poiana, M. A., et al. (2018) Synergistic Antifungal, Allelopatic and Anti-Proliferative Potential of Salvia officinalis L., and Thymus vulgaris L. Essential Oils. Molecules. 16;23 (1).

Alves, F. C. B., Barbosa, L. N., Andrade, B., Albano, M., Furtado, F. B., Marques Pereira, A. F., et al.(2016). Short communication: Inhibitory activities of the lantibiotic nisin combined with phenolic compounds against Staphylococcus aureus and Listeria monocytogenes in cow milk. Journal of dairy science. 99 (3):1831-6.

Araujo, M. J. C., Camara, C., Moraes, M. M., & Born, F. S. (2020) Insecticidal properties and chemical composition of Piper aduncum L., Lippia sidoides Cham. and Schinus terebinthifolius Raddi essential oils against Plutella xylostella L. Anais da Academia Brasileira de Ciencias. 2020;92 (suppl 1):e20180895.

Aryal, B., Niraula, P., Khadayat, K., Adhikari, B., Khatri Chhetri, D., Sapkota, B. K., et al. (2021) Antidiabetic, Antimicrobial, and Molecular Profiling of Selected Medicinal Plants. Evidence-based complementary and alternative medicine : eCAM2021:5510099.

Barbosa, R., Cruz-Mendes, Y., Silva-Alves, K. S., Ferreira-da-Silva, F. W., Ribeiro, N. M., Morais, L. P., et al. (2017) Effects of Lippia sidoides essential oil, thymol, p-cymene, myrcene and caryophyllene on rat sciatic nerve excitability. Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas. 19;50 (12):e6351.

Batista, E. S., Franmir, R. B., Majolo, C., & Kioshi, L. A. K. Lippia alba essential oil as anesthetic for tambaqui. Aquaculture. 495:545-9.

Ben Arfa, A., Combes, S., Preziosi-Belloy, L., Gontard, N., & Chalier, P. (2006) Antimicrobial activity of carvacrol related to its chemical structure. Letters in applied microbiology. 43 (2):149-54.

Botelho, M. A., Nogueira, N. A., Bastos, G. M., Fonseca, S. G., Lemos, T. L., Matos, F. J., et al. (2007) Antimicrobial activity of the essential oil from Lippia sidoides, carvacrol and thymol against oral pathogens. Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas. 40 (3):349-56.

Carvalho, I. T., Estevinho, B. N., & Santos, L. (2016) Application of microencapsulated essential oils in cosmetic and personal healthcare products - a review. International journal of cosmetic science. 38 (2):109-19.

Couto De Oliveira, G., Ferraz, C. S., Andrade Junior, C. V., & Pithon, M. M. (2013) Chlorhexidine gel associated with papain in pulp tissue dissolution. Restorative dentistry & endodontics. 38 (4):210-4.

Dorman, H. J., & Deans, S. G. (2000) Antimicrobial agents from plants: antibacterial activity of plant volatile oils. Journal of applied microbiology. 88 (2):308-16.

Figueredo, C. M., Lira-Junior, R., & Love, R. M.(2019) T and B Cells in Periodontal Disease: New Functions in A Complex Scenario. International journal of molecular sciences. 14;20 (16).

Freires, I. A., Santaella, G. M., de Cassia Orlandi Sardi, J., & Rosalen, P. L.(2018) The alveolar bone protective effects of natural products: A systematic review. Archives of oral biology. 87:196-203.

Ginting, E. V., Retnaningrum, E., & Widiasih, D. A.(2021) Antibacterial activity of clove (Syzygium aromaticum) and cinnamon (Cinnamomum burmannii) essential oil against extended-spectrum beta-lactamase-producing bacteria. Veterinary world. 14 (8):2206-11.

Khruengsai, S., Sripahco, T., Rujanapun, N., Charoensup, R., & Pripdeevech, P.(2021) Chemical composition and biological activity of Peucedanum dhana A. Ham essential oil. Scientific reports. 27;11 (1):19079.

Maragoni, S. T., Moraes, S. H., Utrera, L. A., & Casemiro, M. G. M. (2018) Diterpenes of the pimarane type isolated from Viguiera arenaria: promising in vitro biological potential as therapeutic agents for endodontics. Journal of Pharmacognosy and Phytotherapy. 10 (2):34-44.

Melo, J. O., Fachin, A. L., Rizo, W. F., Jesus, H. C., Arrigoni-Blank, M. F., Alves, P. B., et al. (2014) Cytotoxic effects of essential oils from three Lippia gracilis Schauer genotypes on HeLa, B16, and MCF-7 cells and normal human fibroblasts. Genetics and molecular research : GMR 8;13 (2):2691-7.

Mendes, F. S. F., Garcia, L. M., Moraes, T. D. S., Casemiro, L. A., Alcantara, C. B., Ambrosio, S. R., et al.(2020) Antibacterial activity of salvia officinalis L. against periodontopathogens: An in vitro study. Anaerobe. 63:102194.

Morão, R. P., Almeida, A. C., Martins, E. R., & Prates, J. P.(2016) Constituintes Quimicos e Principios Farmacológicos do óleo Essencial de Alecrim Pimenta (Lippia Origanoides). Revista Unimontes Ciientífica. 8 (1):1-8.

Moreti, D. L. C., Leandro, L. F., da Silva Moraes, T., Moreira, M. R., Sola Veneziani, R. C., Ambrosio, S. R., et al.(2017) Mikania glomerata Sprengel extract and its major compound ent-kaurenoic acid display activity against bacteria present in endodontic infections. Anaerobe47:201-8.

Nagoor Meeran, M. F., Javed, H., Al Taee, H., Azimullah, S., & Ojha, S. K. (2017) Pharmacological Properties and Molecular Mechanisms of Thymol: Prospects for Its Therapeutic Potential and Pharmaceutical Development. Frontiers in pharmacology. 8:380.

Nazzaro, F., Fratianni, F., De Martino, L., Coppola, R., & De Feo, V. (2013) Effect of essential oils on pathogenic bacteria. Pharmaceuticals. 25;6 (12):1451-74.

Nhu-Trang, T. T., Casabianca, H., & Grenier-Loustalot, M. F. (2006) Deuterium/hydrogen ratio analysis of thymol, carvacrol, gamma-terpinene and p-cymene in thyme, savory and oregano essential oils by gas chromatography-pyrolysis-isotope ratio mass spectrometry. Journal of chromatography A. 3;1132 (1-2):219-27.

Olmedo-Juarez, A., Briones-Robles, T. I., Zaragoza-Bastida, A., Zamilpa, A., Ojeda-Ramirez, D., Mendoza de Gives, P., et al.(2019) Antibacterial activity of compounds isolated from Caesalpinia coriaria (Jacq) Willd against important bacteria in public health. Microbial pathogenesis. 136:103660.

Pithon, M. M., Santana, D. A., Sousa, K. H., & Farias, I. M. (2013) Does chlorhexidine in different formulations interfere with the force of orthodontic elastics? The Angle orthodontist. 83 (2):313-8.

Rosato, A., Vitali, C., Laurentis, N., Armenise, D., & Milillo, M. A. (2007) Antibacterial Effect of Some Essential Oils Administered Alone Or In Combination With Norfloxacin. Phytomedicine : International journal of phytotherapy and phytopharmacology. 14:727-32.

Sampaio, G. M., de Meneses, I. H., de Carvalho, F. G., Carlo, H. L., Munchow, E. A., Barbosa, T. S., et al.(2020) Antimicrobial, mechanical and biocompatibility analysis of chlorhexidine digluconate-modified cements. Journal of clinical and experimental dentistry. 12 (2):e178-e86.

Smith, F. L., & Baumgarth, N.(2019) B-1 cell responses to infections. Current opinion in immunology. 57:23-31.

Wijesundara, N. M., & Rupasinghe, H. P. V.(2019) Bactericidal and Anti-Biofilm Activity of Ethanol Extracts Derived from Selected Medicinal Plants against Streptococcus pyogenes. Molecules. 24;24 (6).

Woo, H. G., Chang, Y. K., Lee, J. S., & Song, T. J.(2021) Association of Periodontal Disease with the Occurrence of Unruptured Cerebral Aneurysm among Adults in Korea: A Nationwide Population-Based Cohort Study. Medicina. 30;57 (9).

Xu, J., Wu, C., Yang, Z., Liu, W., Chen, H., Batool, K., et al.(2020) For: Pesticide biochemistry and physiology recG is involved with the resistance of Bt to UV. Pesticide biochemistry and physiology. 167:104599.

Publicado

14/05/2022

Cómo citar

GUSMÃO, I. C. C. P. .; PITHON, M. M. .; SANTOS , A. E. dos .; SILVA , T. S. .; SAMPAIO, F. C. . Actividad antibacteriana de Lippia sidoides Cham frente a periodontopatógenos: estudio in vitro. Research, Society and Development, [S. l.], v. 11, n. 7, p. e3311727141, 2022. DOI: 10.33448/rsd-v11i7.27141. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/27141. Acesso em: 24 dic. 2024.

Número

Sección

Ciencias de la salud