Immunohistological insight into the correlation between cardiac and lingual musculature in chronic chagas disease
DOI:
https://doi.org/10.33448/rsd-v11i5.28156Keywords:
Doença de Chagas; Citocinas; Língua; T. cruzi.Abstract
Aim: To assess and compare the inflammatory infiltrate, area of muscle cells, microvessel density (MVD) and expression of cytokines in the myocardium (MM) and lingual (LM) musculature between chagasic and non chagasic individuals. Methods: Fragments of MM and LM from autopsied chronic chagasic (CC) (n=18) and non chagasic individuals (n=24) were histologically processed for histochemical and immunohistochemical evaluation. Was assessed the MVD by CD31 and quantification of immune cells infiltrated in MM and LM, and also expression of IL-1, IL-10 and TGF- β1. Results: There were higher intensity of inflammation and larger area of muscle cells in the myocardium and tongue of chagasic compared to non-chagasic individuals (p<0.0001). In the chagasic individuals, there was a positive correlation: regarding to the MVD between the LM and MM (p=0.049); between area of MM and density of leukocytes expressing TGF-β1 (p=0.049); and IL-10 correlate with the MM and LM (p=0.029). Conclusions: The correlation between the density of leukocytes immunostained by anti-TGF-β1 and hypertrophy in the myocardium suggests the participation of this cytokine in cardiac muscle hypertrophy. Moreover, the hypertrophy in the myocardium and lingual muscles, and the concomitant expression of CD31 and IL-10 between the myocardium and lingual musculature in chagasic individuals suggests that evaluation of the tongue musculature presents certain similarities changes as a myocardium muscle in CC. However, it needs more studies to verify in other phases of the disease, if the parasite is installed in the tongue or if the mechanisms are reflex of systemic changes in level of immune response.
References
Andrade, L.O., Galvão, L.M., Meirelles, M.N., Chiari, E., Pena, S.D., Macedo, A.M. (2010). Differential tissue tropism of Trypanosoma cruzi strains: an in vitro study. Memorias do Instituto Oswaldo Cruz, 105, 834-837.
Borges, L.F., Caldini, E.G., Battlhener, C.N., Garcia, S.B., Zucoloto, S., Montes, G.S., Taboga, S.R. (2008). Differential distribution of some extracellular matrix fibers in an experimentally denervated rat megaileum. Micron. 39, 397-404.
Cardoni, R.L., Antúnez, M.I., Abrami, A.A. (1999). TH1 response in the experimental infection with Trypanosoma cruzi. Medicina (Buenos Aires). 2, 84-90.
Cardillo, F., Pinho, R.T., Antas, P.R.Z., Mengel, J. (2015) Immunity and immune modulation in Trypanosoma cruzi infection. FEMS Pathogens and Disease, 73(9), 1-
Chagas, C. (1909). New human tripanozomiaze. Mem. Inst. Oswaldo Cruz. 1, 3–71.
de Araújo, F.F., Vitelli-Avelar, D.M., Teixeira-Carvalho, A., Antas, P.R., Assis S.G.J., Sathler-Avelar, R., Rocha, O.C.M., Elói-Santos, S.M., Pinho, R.T., Correa-Oliveira, R., Martins-Filho, O.A. (2011). Regulatory T cells phenotype in different clinical forms of Chagas' disease. PLOS Neglected Tropical Diseases. 5, e992.
de Lima Pereira, S.A., Rodrigues, D.B.R., Ferraz, L.M.F., Castro, E.C.C., Reis, M.A., Teixeira, V.P.A. (2006). Inflammation and glandular duct dilatation of the tongue from patients with chronic Chagas disease. Parasitology Research. 98: 153-156.
de Lima Pereira, S.A., Severino, V.O., Kohl, N.L.M., Rodrigues, D.B.R., Alves, P.M., Clemente-Napimoga, J.T., dos Reis, M.A., Teixeira, V.P., Napimoga, M.H. (2009). Expression of cytokines and chemokines and microvasculature alterations of the tongue from patients whit chronic Chagas' disease. Parasitology Research. 105, 1031-1039.
Dhiman, M., Garg, N.J. (2011). NADPH oxidase inhibition ameliorates Trypanosoma cruzi-induced myocarditis during Chagas disease. The Journal of Pathology, 225, 583-596.
dos Santos, C.M., Cassiani, R.A., Dantas, R.O. (2011). Videofluoroscopic evaluation of swallowing in Chagas' disease. Dysphagia, 26, 361-365.
Dutra-Walderez, O., Hojo-Souza, N.S., Gollob, K.J. (2013). The Immune Response In Chagas Disease And Its Role In The Variability Of Clinical Expression. Revista Española de Salud Pública, 87, 25-32
Ferreira, C.S., Lopes, E.R., Chapadeiro, E., de Oliveira, A.H., de Souza, W.F., da Silva Neto, I.J. (1980). Post-mortem coronary angiography in chronic Chagas carditis, Anatomo-radiologic correlation. Arquivos Brasileiros de Cardiologia. 34, 81-86.
Gutierrez, F.R., Mariano, F.S., Oliveira, C.J., Pavanelli, W.R., Guedes, P.M., Silva, G.K., Campanelli, A.P., Milanezi, C.M., Azuma, M., Honjo, T,, Teixeira, M.M., Aliberti, J.C., Silva, J.S. (2011). Regulation of Trypanosoma cruzi-induced myocarditis by programmed death cell receptor 1. Infection and Immunity. 79, 1873-1881.
Jorge, P.A. (1973). Ischemic cardiopathies. Study of the heart capillaries by electronic microscopy. Arquivos Brasileiros de Cardiologia. 26, 189-206.
Laucella, S.A., Riarte, A., Prado, N., Zapata, J., Segura, E.L. (2001). Alpha 4 Integrins and sialyl Lewis x modulation in chronic Chagas disease: further evidence of persistent immune activation. Scandinavian Journal of Immunology, 53, 514-519.
Lugo de Yarbuh, A., Colasante, C., Alarcón, M., Moreno, E. (2006). Gastrocnemius skeletal muscle, microvasculature and neuromuscular junction alterations in mice with experimental acute Chagas infection. Revista Científica Universidade Del Zulia. 16, 593-603.
Martins-Melo, F.R., Lima, M.A.S., Ramos, A.N., Alencar, C.H., Heukelbach, J. (2014). Systematic review: Prevalence of Chagas disease in pregnant women and congenital transmission of Trypanosoma cruzi in Brazil: a systematic review and meta-analysis. Tropical Medicine & International Health. 19(8), 943-57.
Medeiros, G.A., Silvério, J.C., Marino, A.P., Roffê, E., Vieira, V., Kroll-Palhares, K., Lannes-Vieira, J. (2009). Treatment of chronically Trypanosoma cruzi-infected mice with a CCR1/CCR5 antagonist (Met-RANTES) results in amelioration of cardiac tissue damage. Microbes and Infection, 11, 264-273.
Mizzaci, C.C., Souza, T.G.S.E., Targueta, G.P., Tótora, A.P.F., Mateos, J.C.P., Mateos, J.C.P. (2017). Pacemaker Implants in Children and Adolescents with Chagas Disease in Brazil: 18-Year Incidence. Arquivos Brasileiros de Cardiologia. 108(6), 546-551.
Park, S., Dimaio, T.A., Scheef, E.A., Sorenson, C.M., Sheibani, N. (2010). PECAM-1 regulates proangiogenic properties of endothelial cells through modulation of cell-cell and cell-matrix interactions. American Journal of Physiology-Cell Physiology, 299, 1468-1484.
Petersen, C.A., Burleigh, B.A. (2003). Role for interleukin-1 beta in Trypanosoma cruzi-induced cardiomyocyte hypertrophy. Infection and Immunity. 71, 4441-4447.
Regueiro, A., García-Álvarez, A., Sitges, M., Ortiz-Pérez, J.T., de Caralt, M.T., Pinazo, M.J., Posada, E., Heras, M., Gascón, J., Sanz, G. (2013). Myocardial involvement in Chagas disease: Insights from cardiac magnetic resonance. International Journal of Cardiology. 165(1), 107-12.
Reis, D.D, Jones, E.M., Tostes, S., Lopes, E.R., Chapadeiro, E., Gazzinelli, G., Colley, D.G., McCurley, T.L. (1993). Expression of major histocompatibility complex antigens and adhesion molecules in hearts of patients with chronic Chagas' disease. American Journal of Tropical Medicine and Hygiene, 49, 192-200.
Rosenkranz, S. (2004). TGF-beta1 and angiotensin networking in cardiac remodeling. Cardiovascular Research, 63, 423-432.
Savino, W., Villa-Verde, D.M., Mendes-da-Cruz, D.A., Silva-Monteiro, .E, Perez, A.R., Aoki Mdel, P., Bottasso, O., Guiñazú, N., Silva-Barbosa, S.D., Gea, S. (2007). Cytokines and cell adhesion receptors in the regulation of immunity to Trypanosoma cruzi. Cytokine & Growth Factor Review, 18, 107-124.
Sousa, G.R., Gomes, J.A.S., Damasio, M.P.S., et al. (2017). The role of interleukin 17-mediated immune response in Chagas disease: High level is correlated with better left ventricular function. PLoS One, 12(3), e0172833.
Tanowitz, H.B., Kirchhoff, L.V., Simon, D., Morris, S.A., Weiss, L.M., Wittner, M. (1992). Chagas' disease. Clinical Microbiology Reviews. 5, 400–419.
Torres, S.H., Finol, H.J., Montes de Oca, M., Vásquez, F., Puigbó, J.J., Loyo, J.G. (2004). Capillary damage in skeletal muscle in advanced Chagas' disease patients. Parasitology Research. 93, 364-368.
Vilas-Boas, F., Feitosa, G.S., Soares, M.B., Pinho-Filho, J.A., Mota, A.C., Almeida, A.J., Andrade, M.V., Carvalho, H.G., Oliveira, A.D., Ribeiro-dos-Santos, R. (2011). Bone marrow cell transplantation in chagas' disease heart failure: report of the first human experience. Arquivos Brasileiros de Cardiologia. 96, 325-331.
Wang, Y., Moreira, M.D.A., Heringer-Walther, S., Khan, A., Schultheiss, H.P., Wessel, N., Siems, W.E., Walther, T. (2011). Does the aminopeptidase a have prognostic and diagnostic value in Chagas disease and other dilated cardiomyopathies? Journal of Cardiovascular Pharmacology. 58, 374-379.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2022 Marcela Beghini; Douglas Reis Abdalla; Janainna Grazielle Pacheco Olegario; Taíssa Cássia de Souza Furtado; Juliana Barbosa de Faria; Denise Bertulucci Rocha Rodrigues; Ruchele Dias Nogueira; Sanívia Aparecida de Lima Pereira
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
1) Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
2) Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
3) Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.
