Antitumor and human topoisomerase II-α inhibitory activity II-α of the flavonoid luteolin isolated from Prosopis juliflora
DOI:
https://doi.org/10.33448/rsd-v11i5.28220Keywords:
Antitumor activity; Prostate cancer; Natural products; Prosopis juliflora.Abstract
This study aimed to evaluate the antitumor and inhibitory activity of human topoisomerase II-α of the flavonoid luteolin isolated from Prosopis juliflora against prostate tumor cells (PC-3), with the aim of investigating new drugs of natural origin. In vitro tests were conducted with crude leaf extracts against PC-3 and FGH cell lines. Cytotoxicity tests were performed using MTT (3-(4,5-dimethiazol-2yl) 2,5-diphenyl tetrazolium bromide) with monitoring after 24 h of exposure of tumor and non-tumor cells to different concentrations of extracts (100; 50; 25; 10 μg/mL). Luteolin extracted from the leaves of P. juliflora showed positive results on the DNA topo II-α enzyme essential in the replication process of cancer cells from the 50 µM concentration. Furthermore, it showed antitumor activity against PC-3 cells from the lowest concentration tested 10 µM, and absence of cytotoxicity against normal cells. A chemical study for the extraction and isolation of active compounds from P. juliflora leaf extracts is suggested, to investigate the possible antitumor activity of these compounds in in vitro and in vivo assays.
References
Ahmad, R., Ahmad, R., Ahmad, N., Naqvi, A. A., Shehzad, A., & Al -Ghamdi, M. S. (2017) Role of traditional Islamic and Arabic plants in cancer therapy. Journal of traditional and complementary medicine, 7, 2, 195-204.
Amaria, N. R., Menzie, A. M., Burton, E. M., Scolyer, R. A., Tetzlaff, M. T., Antdbacka, R., Ariyan, C., Bassett, R., Carter, B., Daud, A., Faries, M., Fecher, L. A., Flaherty, K. T., Gershenwald, J. E., Hamid, O., Hong, A., Kirkwood, J. M., Lo, S., Margolin, K., Messina, J., Michael, A. P., M. A., Rizos, H.,Ross, M. I., Saw, E. A. R. R. P.M., Sondak, V., Sullivan, R. J.,Taube, J. M., Thompson, J. F., Wiel, B. A. V. W. & Eggermont, A. M. (2019). Neoadjuvant systemic therapy in melanoma:recommendations of the International Neoadjuvant Melanoma Consortium. Lancet Oncology. 20, 7, 378-389.
Branco, A., Pinto, A. C., Braz-Filho, R., Silva, E. F., & Grynberg, N. F. (2008) Echevarria, A. Rubrofusarina, um policetídeo natural inibidor da topoisomerase II-α humana. Rev. bras. farmacogn. 18, 1, 703-708.
Bray, F., Ferlay, J., Soeriomataram, I., Siege, R. L., Torre, L. A., & Jemal, A. (2018) Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA: a cancer journal for clinicians, 68, 6, 394-424.
Brasil. Ministério Da Saúde. Instituto Nacional De Câncer José Alencar Gomes Da Silva (2020). Coordenação de Prevenção e Vigilância. Estimativa 2020: incidência de câncer no Brasil. INCA.
Gurushidhappa, U. B., Shivajirao, P. M., Vasudeo, N. P., Shankar, K. S., & Nivarti G.R. (2018) Prosopis juliflora (Sw.), DC induz apoptose e parada do ciclo celular em células de câncer de mama triplo-negativas: investigações in vitro e in vivo. Oncotarget, 9, 1, 30304-30323.
Hande K.R. Topoisomerase II inhibitors. Update Canc Therap, 3,1, 3-15,2008.
Hong, Z., Cao, X., Li, N., Zhang, Y., Lan, L., Zhou, Y., Pan, X., Shen, L., Yin, Z., & Luo, L. (2014). Luteolin is effective in the non-small cell lung cancer model with L858R/T790M EGF receptor mutation and erlotinib resistance. British journal of pharmacology, 171(11), 2842–2853.
Krug, F. P. M. & Cavalcanti, G. (2018) Conhecimento e Atitudes sobre o Câncer de Próstata no Brasil: Revisão Integrativa. Revista Brasileira de Cancerologia, 64(4): 561-567
Lee, E. J., Oh, S. Y., & Sung, M. K. (2012) Luteolin exerts anti-tumor activity through the suppression of epidermal growth factor receptor-mediated pathway in MDA-MB-231 ER-negative breast cancer cells. Food and Chemical Toxicology, 50(11), 4136-4143.
López- Franco, Y. L., Cervantes-Montano, Martínez- Robinson, K. G., Lizardi-Mendoza, J., & Robles-Ozuna, L. E. (2013) Physicochemical characterization and functional properties of galactomannans from mesquite seeds (Prosopis spp.). Food Hydrocolloids, 30, 2, 656-660.
Mosmann, T. (1983) Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Journal of Immunology Methods, 16, 65, 55-63.
Mota, D. K. A. S., Gonzaga, L. S., Carmo, J. F. M., Ribeiro, J. B.C., Souza, R. B. L., Oliveira, T. L. S., & Santos, E. N. (2004) Plantas medicinais indicadas como anti-inflamatórios por “raizeiros” da região de Goiânia. Infarma, 16, 1-2, 81-82.
Nascimento, C. E. S., Tabarelli, M., & Da Silva, C. A. D. (2014) The introduced tree Prosopis juliflora is a serious threat to native species of the Brazilian Caatinga vegetation. Science of The Total Environment, 481, 0, 08-113.
Oliveira, L. P., Pinheiro, C. R., Vieira, M. S., Paula, J. R., Bara, & Valadares, M. C. (2010) Atividade citotóxica e antiangiogênica de Punica granatum L., Punicaceae, Rev. bras. farmacogn. 20, 2. 201-207.
Prabha D., Mani P., Malliga P., & Darms H. U. (2017) Phytochemical and pharmacological analysis of ethanolic extracts of Prosopis juliflora (S.W). World J Pharm Sci. 6, 0, 2059- 2069.
Pretti K., Sharma R. A. J., & Mala A. (2017) isolation and identification of flavonoids from Prosopis julifloraMint Jour of Pharma and Medical Scien, 6:1-3.
Rosa, C., Câmara, S. G., & Béria, J. U. (2011) Representações e intenção de uso da fitoterapia na atenção básica à saúde. Ciências & Saúde Coletiva,16, 1, 311 - 318, 2011.
Sampaio Filho C., Bertoni V.D., Sampaio, C., Pimenta, A., & Brandão, M. A. (2006) Agentes antineoplásicos. In: Penildon, S. (org.) Farmacologia. (7a ed.) Guanabara Koogan, 1055-1070.
Shanmugam, S., Thangaras, P., Lima, B. S., Chandran, R., Araujo, A. A. S. Narain, N., Serafini, M. R., Quintas-Junior, L. J. (2016) Effects of luteolin and quercetin 3-B-D-glucoside identified from Passiflorasubpeltata leaves against acetaminophen induced hepatoxicity in rats. Biomed Pharmacother, 83, 10, 1278- 1285.
Viana, A. R., Marzari, J., Wergutz, J., & Krause, L. M. F. (2017). Produtos bioativos na prevenção e no tratamento do Câncer, em especial o melanona. Disciplinarum Scientia, 18, 3, 511-528.
Urban, K., Mehrmal, S., Uppal P., Giesey, R. L. & Delost, G. R. (2021). The global burden of skin cancer: a longitudinal analysis of the Global Burden of Disease Study, 1990-2017. JAAD International, 2, 3 98-108.
Yip, K. T., Zhong, Y. Z., Putz, N. S. S., Autzen, J., Gasper, R., Hofmann, E., Scherkenbeck, J., & Stoll, R. (2016). Small molecules antagonize the MIAFibronectin interaction in malignant melanoma. Scientific Reports, 6, 25119, 1-12.
Wu, B., Zhang, Q., Shen, W. & Zhu, J. (2008) Efeitos anti-proliferativos e quimiossensibilizantes da luteolina na linhagem celular AGS de câncer gástrico humano. Mol Cell Biochem, 313 (4) 125-132.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2022 Rosiane Aleixo Diniz; Adrielle Antônia da Silva; Giani Maria Cavalcante; Luiz Andrade Lins
This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
1) Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
2) Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
3) Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.
