In vitro antifungal activity of aqueous extracts of olive bagasse (Olea europaea L.) against fungal that cause candidiasis, dermatophytosis and sporotrichosis in humans and animals

Authors

DOI:

https://doi.org/10.33448/rsd-v11i6.29090

Keywords:

Aqueous extract; Oleuropein; Hydroxytyrosol; Cytotoxicity.

Abstract

Fungal infections have become frequently in recent years. Dermatophytosis, sporotrichosis and candidiasis stand out. Due to the resistance to available drugs and with a view to diversifying the pharmaceutical field, the study was carried out using the bagasse of O. europaea. Studies related to olive trees do not address bagasse, which represents a potential economic alternative to its discarded tons by the industry. The objective of this work was to evaluate the in vitro sensitivity profile of fungal isolates against aqueous extracts of O. europeae bagasse. To test the cellular toxicity of the extracts and to determine the presence and amount of the main phenolic compounds. No antifungal activity of these aqueous extracts was observed against the isolates of dermatophytes and Sporothrix brasiliensis. The Candida spp. tested for in vitro susceptibility, two were sensitive to bagasse decoction extract from a concentration of 50mg/ml of DEC 10'. In the toxicity of the olive pomace decoction, concentrations from 12.5 mg/ml allowed at least 50% of the MDBK cells to remain viable. Concentrations from 3.13 to 0.78 mg/ml were safe, as 100% cell viability occurred. Of the hydroxytyrosol (HPLC) compounds, the concentration varied from 8 x10-4 to 34 x10-4 mg/ml in INF 10' and from 11 x10-4 to 81 x10-4 mg/ml in DEC 10'. For oleuropein, the DEC 10' extracts varied their concentration from 87 x10-3 to 7 x10-3 mg/ml, while for INF 10' the values ​​were from 23 x10-3 mg/ml to not detected in some varieties.

Author Biographies

Márcia Kutscher Ripoll, Universidade Federal do Rio Grande do Sul

Pós-graduanda do Programa de Pós-Graduação em Ciências Veterinárias pela Universiade Federal do Rio Grande do Sul (UFRGS).

Otávia de Almeida Martins, Universidade Federal de Pelotas

Assistente de laboratório da Universidade Federal de Pelotas (UFPEL), desenvolve as atividades no Centro de Diagnostico em Micologia Veterinária na FaVet.

Stefanie Bressan Waller, Universidade Federal de Pelotas

Pós-doutorado Júnior (CNPq).

Anna Luiza Silva, Universidade Federal de Pelotas

Mestre em Ciências Animais pela universidade Federal de Pelotas (UFPel), atualmente empreendedora e empresária autônoma na área de Medicina Veterinária. 

Renata Osório de Faria, Universidade Federal de Pelotas

Professora Adjunto da Faculdade de Veterinária da Universidade Federal de Pelotas (UFPel)

Angelita dos Reis Gomes, Universidade Federal de Pelotas

Médica Veterinária responsável tècnica no Centro de Diagnóstico e Pesquisa em Micologia Veterinária (MicVet).

Tony Picoli, Faculdade de Americana; Universidade São Francisco

Professor da Faculdade Americana e da Universidade São Francisco, atando na àrea de Medicina Veterinária e Fisioterapia.

Mário Carlos Araújo Meireles, Universidade Federal de Pelotas

Prestador de serviço acadêmico voluntário, orientador do programa de Pós-graduação em Veterinária da Universidade Federal de Pelotas e pesquisador Bolsista 1C do Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)

Tanize dos Santos Acunha, Universidade de Ribeirão Preto

Pós-doutorado na Universidade de Ribeirão Preto e responsável por análises de fitoterápicos da unidade da Farmácia Viva "Farmácia da Natureza da Terra de Ismael" localizada em Ribeirão Preto.

Fabio Clasen Chaves, Scotts Miracle-Gro

Professor adjunto do DCTA-FAEM-UFPel, atualmente em licença. Trabalhando na Scotts Miracle-Gro como Analitycal Scientist.

João Roberto Braga de Mello, Universidade Federal do Rio Grande do Sul

Professor titular da Universidade Federal do Rio Grande do Sul (UFRGS) e Médico Veterinário do Hospital de Clínicas Veterinárias da UFRGS.

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Published

27/04/2022

How to Cite

RIPOLL, M. K. .; MARTINS, O. de A. .; WALLER, S. B. .; SILVA, A. L. .; FARIA, R. O. de .; GOMES, A. dos R. .; PICOLI, T.; MEIRELES, M. C. A. .; ACUNHA, T. dos S. .; CHAVES, F. C. .; MELLO, J. R. B. de . In vitro antifungal activity of aqueous extracts of olive bagasse (Olea europaea L.) against fungal that cause candidiasis, dermatophytosis and sporotrichosis in humans and animals . Research, Society and Development, [S. l.], v. 11, n. 6, p. e26111629090, 2022. DOI: 10.33448/rsd-v11i6.29090. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/29090. Acesso em: 3 jul. 2022.

Issue

Section

Agrarian and Biological Sciences