Green synthesis of chitosan by Cunninghamella elegans UCP 1306 using sustainable substrates mediated morphological changes




Agroindustrial waste; Biopolymers; Fungal Chitosan; Cunninghamella elegans.


Currently, the commercial production of chitosan takes place through the thermochemical deacetylation of crustacean shells, which process requires the use of chemical substances, such as strong alkaline solutions, which are important sources of environment pollution. We present a method for the production of chitosan by Cunninghamella elegans UCP 1306 using a green metabolic conversion of agro-industrial residues, based on a 22 Factorial Design and describe the main morphological changes observed in the specie. The highest yield of biomass (6.375 g/L) and chitosan (101.7 mg/g) were observed in the assay 2 (Corn steep liquor -CSL 4%, Cassava wastewater-CWW 4%), where the specie presented loose and branched hyphae with the presence of septations, pellets of 0.4 to 0.5 mm and clumps of up to 0.1 mm. Statistical analysis showed that higher concentration of CSL contributed significantly to the growth of the species. The bands determined in Fourier transform infrared spectroscopy confirmed the degree of deacetylation of 84.61% of the chitosan obtained. This research showed that the use of CSL and CWW in association was very promising, being able to be used as sustainable sources of nitrogen and carbon in the production of fungal chitosan with a high degree of deacetylation and can be applied to the industrial process.

Author Biographies

Ákylla Fernanda Souza Silva, Universidade Católica de Pernambuco

Nucleous of Research in Environmental Sciences and Biotechnology

Adriana Ferreira de Souza, Universidade Católica de Pernambuco

Nucleous of Research in Environmental Sciences

Irapuan Oliveira Pinheiro, Universidade de Pernambuco

Biological Sciences Course

Galba Maria de Campos-Takaki, Universidade Católica de Pernambuco

Nucleus of Research in Environmental Sciences


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How to Cite

SILVA, Ákylla F. S.; SOUZA, A. F. de; PINHEIRO, I. O. .; CAMPOS-TAKAKI, G. M. . de . Green synthesis of chitosan by Cunninghamella elegans UCP 1306 using sustainable substrates mediated morphological changes. Research, Society and Development, [S. l.], v. 11, n. 7, p. e38211729387, 2022. DOI: 10.33448/rsd-v11i7.29387. Disponível em: Acesso em: 2 mar. 2024.



Agrarian and Biological Sciences