Immunohistochemical and histomorphometric analysis of the endometrium of rats in permanent estrus treated with melatonin, metformin and clomiphene citrate




Endometrium; Permanent estrus; Health teaching; Melatonin; Metformin; Clomiphene citrate; Rats.


Objective: To evaluate the effects of melatonin, metformin and clomiphene citrate on the endometrium of polycystic ovary syndrome model rats. Methods: Seventy-eight rats were divided into 13 groups and two experiments (prevention and treatment). After exposure, all animals were anesthetized and the uterine horns were removed and submitted to hematoxylin and eosin staining and immunohistochemistry (Ki-67 and Vegf). The data obtained were submitted to Student's t test (p<0.05). Results/Conclusion: Melatonin, metformin and clomiphene citrate do not promote proliferative and angiogenic effects on the endometrium of rats in permanent estrus, however, melatonin has a more significant trophic effect on the endometrial stroma, which did not interfere with its thickness, while that metformin had a more significant trophic effect on endometrial glandular epithelium, therefore, these effects are similar both before and after PCOS induction by continuous light exposure.


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How to Cite

MATTOS, L. S. de; LOMBARDI , L. A. .; MOURA, M. L. A. .; ESPINDULA, A. P.; SIMÕES, R. S. .; FLORENCIO-SILVA, R. .; FRANCO, P. C. .; SASSO, G. R. da S. .; SIMÕES, M. de J. . Immunohistochemical and histomorphometric analysis of the endometrium of rats in permanent estrus treated with melatonin, metformin and clomiphene citrate. Research, Society and Development, [S. l.], v. 11, n. 7, p. e28011729995, 2022. DOI: 10.33448/rsd-v11i7.29995. Disponível em: Acesso em: 23 may. 2024.



Health Sciences