Ozone therapy as an ally in aesthetic treatment in skin rejuvenation





Health teaching; Ozone; Ozone therapy; Aesthetics; Oxidant; Rejuvenation; Biostimulation; Skin.


Introduction: Ozone therapy is an ancient practice, used in the treatment of some diseases, but for some years, many specialists are inserting ozone therapy in aesthetics. Objectives: To carry out an integrative review of the literature on the importance of ozone therapy in skin rejuvenation. Methodology: An integrative review was carried out in the PUBMED, ISCO 3 and SCIELO databases, published between 2011 and 2021, using some descriptors involving ozone therapy and aesthetics. Results: We found 50% (10/20) of clinical trials that deal with the power of ozone and its mechanism in tissue regeneration and healing in injuries caused by different natures; and 50% (10/20) of review articles that addressed: the use of ozone gas to increase circulation and response to oxidative stress; bactericidal and antifungal activity; the fundamental role in aesthetic treatments. Conclusion: In this research, it was verified that ozone therapy, both in animal and human models, has properties in aesthetic dysfunctions, such as localized fat, stretch marks, hyperchromasia, alopecia, sagging and skin rejuvenation, due to its power in combating radicals. cell proliferation, collagen biostimulation, fibroblast biostimulation and anti-inflammatory activity, helping in the general appearance of the skin of the body and face, as well as scarring.


Lacerda, A. C. & Grillo, R. (2022). Efficacy of biostimulating ozone therapy: case report literature review. J Rounesp 50; 34-42.

Al Habashneh, R., Alsalman, W. & Khader, Y. (2016) Ozone as an adjunct to conventional non surgical therapy in chronic periodontitis: a randomized controlled clinical trial. J Periodontal Res 50, 37-43.

Albertini, F. & Bonetti, M. (2006). Intraforaminal O2-O3 infiltration: use of CT guidance in case facci- dental puncture of the peri ganglionic venous plexus. Revital Ossigeno Ozono Ter 5, 21-6.

Andreula, C. F. et al. Minimally Interventional Spinal procedures. Eur J Radiol 50, 112-9 (2004).

Biondi, G. et al. (1997) E. Reactivity Of Nucleic Acids With ozone: an FT-IR micrsospec- troscopystudy. App. Spectroscopy 51.

Bocci, V. et al. (2011) ValidityofOxygen-Ozone Therapy as IntegratedMedicationForm in ChronicInflammatoryDiseases. 6-84.

Bocci, V. (2008).Biological And Clinical Effects Of ozone. Has ozone therapy a future.23.

Bocci, V.(2008). Does ozone really "cure" cancer? Int J Cancer 123, 1222.

Bocci, V. et al. (1999). Ozonation Of Blood During Extracorporeal Circulation. I. Rationale, methodology and preliminary studies. Int J Artif Organs 22, 645-51.

Bocci, V. et al (1999). Studies On The Biological Effects Of Ozone: 9. Effects of ozone -82.

Bocci, V. (2006) Is it truethat ozone is always toxic? The end of a dogma. ToxicolApplPharmacol 216, 493- 504.

Bocci, V. (2002). Oxygen-Ozone therapy. A critical evaluation, 4 The Netherlands: KluwerAcademicPublishers 440.

Bocci, V. Ozone. (2005). A New Medical Drug, Springer, Dordrecht, The Nederlands,295.

Brauner, A. Clinicalstudiesoftherapeuticresultsfromozonizedwater for Campanati, A. et al. Topical ozonated oil versus hyaluronic gel forCardiovascHematolDisordDrug Targets.;15(2):127-38 (2015).

Cardoso, M.G; et al .(2008), . EffectivenessofozonatedwateronCandida albicans, Enterococcus Faecalis, and endotoxins in root canals. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 105, e85-91.

Oliveira M, F.N; et al. (2012). Ozone Therapy as a Treatment for Low Back Pain Secondary Herniated Disc: A Systematic Review and Meta-analy- sisofRandomizedControlledTrials. PainPhysician 15, E115-29.

Gaggiotti, E. & Galli, F. (2005) Extracorporeal Blood Oxygenation And Ozonation: clinical and biological implications of ozone therapy. Redox Rep 10, 121-30.

Bocci, V. Gaggiotti, (2004) E. Ozone therapy. Int J Artif Organs 27, 168-75.

Dialysis devices usable as ozo- ne gas exchangers? ArtifOrgans 34, 170-5 (2009).

Ebru Saglam & Susan Bayer Alliance, 2019. Evaluation of the effect of topical and systemic application of ozone on periodontitis: an experimental study in rats effect of subgingival ozone and/orhydro- gen peroxide onthedevelopmentofperi implant mucositis: a double-blindrandomizedcontro- lledtrial. Int J Oral MaxillofacImplants 28, 1483-9.

EIGA. European industrial gases association AISBL. ComparisonofEuropean, US & Japanese Pharmacopoeia Monographs for medicinal gases. MGC Doc 152/11/E Revision Of Doc 152/08. Avenue desArts 3-5 B 1210 Brussels. www.eiga.eu Revised . (2011).

Hadary, A. (2011). Evaluationoftheeffectofozonatedplantoilsonthequalityofosseointegrationof dental implants under the influence of cyclosporin: an in vivo study. J Oral Implantol 37, 24757.

Karakaya, E. & Akdur, A. 2021. Effect of subcutaneous topical ozone therapy on second-degree burns in rats: an experimental study.

Borges, F. S. & Meyer, P. 2021. Fundamentals of the Use of Ozone Therapy in the Treatment of Aesthetic Disorders: A Review for Medical Doctors. www.isco3.org. (2015).

BloodCells Mol Dis for Medical Doctors. www.isco3.org. (2015). . global prevalenceofgluco- se-6-phosphate dehydrogenase deficiency: a systematic review and meta-analysis.

Guizhi Qin and Jinhua Huang, 2018. Topical application of ozone: an innovative therapy for childhood atopic dermatitis.

A clinical study of 322 cases. 4, 6-8 (2005), herniation by oxygen-ozone injection. http://jpdb.nihs.go.jp/jp15e/ (2006). http://www.isco3.org/. (ISCO 3, Madrid, 2012).

in Practice. Health Manual, Ministry Health Service of The Russian Federation The State Medical AcademyOf Nizhny Novgo- rod, Russia. http://www.absoluteozone.com/assets/ozone_therapy_in_practice.pdf, (2008).

Cabral, I. L. & Utzig, L. S. 2020. Aqueous ozone therapy improves standard treatment of leishmaniasis lesions in animals leading to local and systemic changes ISCO3. GuidelinesandRecommendations forMedical Professionals Planning toAcquire a Medical Ozone Generator. http://www.isco3.org/offdocs.html. 1 edn (Madrid, 2014).

ISCO3. Learning MethodologyInstructionsandPerfection in Ozone Therapy

ISCO3. Ozone TherapyandLegislation - Analysis for its Regularization. http://www.isco3.org/off- docs.html edn (Madrid, 2015).

Huang, J. & Jinhua Huang, 2018. Topical ozone therapy: an innovative solution for herpes zoster patients. Elservier B.V. 4, 56-59.

Jinrong, Z.& Jianhua D. 2020. Topical ozone therapy restores microbiome diversity in atopic dermatites.

Jinrong Zeng & Jianyun Lu, 2018. Mechanisms of action involved in ozone therapy in skin diseases

Pires, J. R. et al, 2021. Effect of systemic ozone therapy as a biomodulator of tissue regeneration and inflammatory response in rats

Kontorschikova, C. et al. Ozone correctionofmetabolismmisbalanceinducedbyendogenintoxi- cation in patients with burn injury. in IOA 17th World Ozone Congress (ed. IOA, M.T.C.) (Stras- bourg, 2005).

Kronenberg, O., Lussi, A. & Ruf, S. (2009) Preventive Effect Of ozone onthedevelopmentofwhite spot lesions during multibracket appliance therapy. AngleOrthod 79, 64-9.

Kustarci, A., Sumer, Z., Altunbas, D. &Kosum, S. Bactericidaleffectof KTP laser irradiationagainstEnterococcusfaecaliscomparedwithgaseous ozone: anex vivo study. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 107, e73-9 (2009).

Leonardi, M. Disc Puncture under Fluoroscopic Guidance. Revital Ossigeno Ozono Ter 1, 73-8 (2002).

Li, L. Y., Ma, R. L., Du, L., & Wu, A. S. (2017) Ozonatedautohemotherapymodulatestheserumlevelsofinflammatorycytokines in gout patients. Open access rheumatology :research and reviews, 9, 159-165.

Litvinenko, S.V., et al (2008). The efficiency comparative analysis of various ozone therapy techniques for purulent maxillary sinusitis treatment. in Proceedings Of IV Ukrainian-Russian Theoretical And Practical conference, the 3 thAsian- Europeantheoreticalandpracticalconfe- rence "Ozone in biology and medicine" Vol. 5 95-97 (Bulletin of physiotherapy and balneology, ).

LoGiudice, G.et al (2004). Acute bilateral vitreo-retinal hemorragia following oxygen-ozone therapy for lumbar disk herniation. Am J Ophthalmol 138, 175-7.

Lynch, E. (2008). Evidence-basedefficacyof ozone for root canal irrigation. J EsthetRestor Dent 20, 287- 93.

Mingsheng S. & Qinghai Z. 2018. The antibacterial effect of topical ozone in the treatment of MRSA skin infection

. J VascIntervRadiol 21, 534-48.

Morteza, I. & Ramin K. 2019.Efficacy of comprehensive ozone therapy in healing diabetic foot ulcers . J VascIntervRadiol 21, 534-48.

Muto, M. (2004) Intradiscal and Intramuscular Injection of Oxygen-Ozone: PathologicalEvaluation. Work in Progress. Revital Ossigeno Ozono Ter 3, 7-13.

Nagayoshi, M. et al. (2014). Efficacy Of ozone on survival and permeability of oral NIH. National Institutes of Healt NationalCancerInstitute. U.S. Dep.

Nkhoma, E.T., Poole, C., Vannappagari, VHall, S.A. & Beutler, E. The

Noetzel, J. et al. Efficacy Of Calcium Hydroxide, Er:YAG laser orgaseous Nrf2/activation pathway in vivo? A preliminary result. . J VascIntervRadiol 21, 534-48.

OCEBM. LevelsofEvidenceWorkingGroup. http://www.cebm.net/oxford-

of certain hazardous ces in electrical and electronic equipment (EEE) is referred to as RoHS 1 (27 January 2003); and the recast Directive 2011/65/EU isreferredto as RoHS.

Silva, O. & Venâncio, A. 2013. Ozone applications to prevent and degrade mycotoxins: a reviewozonated human serum upregulation of HO-1 in endothelial cells. ToxicolApplPharmacol 267, 30-40.

Pellicano, G., Martinelli, F. Tavanti, V. (2007) The Italian Oxygen-Ozone Therapy Federation (FIO) study Oxygen-Ozone Treatment Herniated disc. Int. J. Ozone Therapy 6, 7-15.

Pharmacology And Toxicology Of Ozone Therapy (2010) Today. J BiolRegulHomeostAgents 10, 3153. pharmacopoeia-7th-edition-1401.

Sanguanini, R. C. & Mariana F. Bento, 2021. Ozonated solutions favor the repair of experimentally induced skin wounds in rats , J LaryngolOtol 123, 54-60.

Ribeiro, R. de Andrade & Olavo Barbosa de Oliveira-Neto, 2019. Efficacy of ozone therapy compared with other therapies for low back pain: a systematic review with meta-analysis of randomized controlled trials

Razumovsky, S.D., Konstantinova, M.L., Grinevich, T.V., Korovina, G.V. &Zaitsev, V.Y. Mechanism Andkineticsofthe Reaction Of ozone with sodium chloride in aqueous solutions. Kinetics And catalysis 51, 492-496 (2010).

J VascIntervRadiol 21, 534-48 Riquet, J., Riquet, C. &Campinchi, R. Suddenonsetofisolated orbital

Sánchez P. et.al. Unidad de tratamiento de dolor: estándares y

Sánchez, G.M.-. Re, L., Perez-Davison, G. & Delaporte, R.H. Las Aplicaciones médicas de los acei- tes ozonizados, atualização. RevEsp Ozonoterapia 2, 121-139 (2012).

Scarchilli, A. & Malpieri, (2004) R. Meninge alirri tatatio na fter paravertebral oxygen ozone injections. A case report. Riv. It. Ossigeno-Ozonot 3, 35-36.

Schulz, S. et al. (2018) Treatmentwith ozone/oxygen-pneumoperitoneum results in

Schwartz, A. (ed.) Guía para el uso médico del ozono. Fundamentos

Seyam, O., Smith, N. L., Reid, I., Gandhi, J., Jiang, W., & Khan, S. A. . Clinical Utility Of ozone therapy for musculoskeletal disorders. Medical gasresearch, 8(3), 103-110.

Shmakova, I.P. &Nazarov, E.I. (2004) Methodsofapplicationof ozone in medicine (guidelines). (The Mi- nistryof Health ofUkraine. Ukrainian centre for scientific medical information and license work., Kiev.

Silva, R.A., Garotti, J.ESilva, R.SNavarini, A. Pacheco, A.M., Jr. Analysis Of The Bactericidal Effect Of Ozone pneumoperitoneum. Acta CirBras 24, 124-7 (2009).

Smith, N. L.et al(2009).. Injection Therapy for subacute and chronic low back pain: an updated Cochrane review. Spine 34, 49-59.

Seng, T. C. & Li, C. (2008) Inactivation Of surface viruses by gaseous ozone. J Environ Health 70, 56-62.

therapy: an overview of pharmacodynamics, current research, and clinical utility. Medical gasresearch, 7(3), 212-219. (2017)

The treatment of acute back pain with lumbar disc herniation: a multicenter, randomized, double-blind, clinicaltrialofactiveandsimu- latedlumbarparavertebralinjection. Spine 34, 1337-44 (2009).

The treatment of partial- to full-thickness second-degree burns: A prospective, comparative, single-blind, non-randomised, controlled clinical trial. Burns 39, 1178-83 (2013).

Trattamento con ossigeno-ozonoterapia paravertebral. Presentazione Di Un caso. Revital Ossigeno Ozono Ter 3, 35-6 (2004).

Travagli, V., et al (2001). Beneficial clinical effects of ozonated autohemotherapy in chronicallydialysedpatientswithatheroscleroticischemiaofthelowerlimbs--pilotstudy. Int J ArtifOrgans 24, 79-82.

Valacchi, G. et al. (2011). Ozon ated sesameoilenhances cutâneo us woundhealing in SKH1 mice. WoundRepairRegen 19, 107-15.

Viebahn-Hänsler, R., Fernández, O.S.L. &Fahmy, Z. (2012) Ozone in Medicine: The Low- Dose Ozone Concept. Guidelines and Treatment Strategies. . J VascIntervRadiol 21, 534-48 34, 408-424.

Weirong Xiao & Hua Tang, 2017. Ozone oil promotes wound healing by increasing fibroblast migration via the PI3K/Akt/mTOR signaling pathway.23-24.

Xiaoqi Wang, 2018. Emerging roles of ozone in skin diseases. J VascIntervRadiol 22.45-50

Zanardi, V. I.et al (2008) Physico-chemical character is a za- tion of sesame oil derivatives. Lipids 43, 877-86.



How to Cite

MACEDO, A. de O. .; LIMA, H. K. F. .; DAMASCENO, C. A. . Ozone therapy as an ally in aesthetic treatment in skin rejuvenation. Research, Society and Development, [S. l.], v. 11, n. 7, p. e44211730141, 2022. DOI: 10.33448/rsd-v11i7.30141. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/30141. Acesso em: 2 mar. 2024.



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