Ultrasound wave transports apitoxin in arthritic joint. - Experimental study

Authors

DOI:

https://doi.org/10.33448/rsd-v11i7.30386

Keywords:

Arthritis induced; Apitoxin; Phonophoresis; Rattus wistar.

Abstract

Man, in his common lore started using the toxin produced by bees, apitoxin, as a topical medication for rheumatoid arthritis in time immemorial. Systematically subjecting themselves to bee stings. In this study, we sought to use this substance, apitoxin, in an experimental model of induced arthritis. In order for the substance to be introduced through the tissue, ultrasound was used as a vehicle, this action is called phonophoresis. So that the apitoxin would reach the arthritic joint. Wistar rats were used in a model where arthritis was induced by inoculation into the knee joint with zymosan. The animals were divided into groups: GI (positive control), GII (negative control), GIII (topical apitoxin-treated group), GIV (ultrasound-treated group) and GV (phonophoresis-treated group). Inducing arthritis, once established, treatment of the groups was initiated. After 21 days of treatment, the animals were anesthetized with ketamine and xylazine, their knees were submitted to radiographic images, temperatures were collected and euthanized. The RX films were processed by Image J software to collect the intra-articular distance of the femur and tibia. The temperature of each individual in the group was collected. Space and temperature averages: Group I (0.15 mm and 35.7°C), group II (0.03 mm and 38.2°C), group III, (0.4 mm and 37.8°C), group IV (0.6 mm and 36.7°C) to group V (0.78 mm and 36.2°C). We conclude that ultrasound combined with apitoxin via phonophoresis was efficient in tissue repair and subsequent regeneration of joint contours when compared with ultrasound or topical apitoxin alone.

References

Acheta, J., Stephens, S. B. Z., Belin, S., & Poitelon, Y.( 2022 ). Therapeutic Low-Intensity Ultrasound for Peripheral Nerve Regeneration - A Schwann Cell Perspective. Front Cell Neurosci. 15:812588. 10.3389/fncel.2021.812588.

Altman, R. D., Dean, D. D., Muniz, O. E., & Howell, D. S. (1989). Prophylatic treatment of canine osteoarthritis with glycosaminoglycan polysulfuric acid ester. Arthritis & Rheumatism, 32, 759-66, 1989a.

Altman, R. D., Dean, D. D., Muniz, O. E., & Howell, D. S. (1989). Therapeutic treatment of canine osteoarthritis with glycosaminoglycan polysulfuric acid ester. Arthritis & Rheumatism, 32, 1300-7, 1989b.

Baranowski, D. C., Buchanan, B., Dwyer, H. C., Gabriele, J. P., Kelly, S., & Araujo, J. A. (2018). Penetration and efficacy of transdermal NSAIDs in a model of acute joint inflammation. Journal of pain research, 11, 2809–2819. https://doi.org/10.2147/JPR.S177967

Beck, B. F. (1997). The bible of bee venom therapy: bee venom, its nature, and its effect on arthritic and rheumatoid conditions. Health Resources Press, 260p.

Bradshaw, J. P., Dempsey, C. E., & Watts, A. (1994). A combined X-ray and neutron diffraction study of selective deuterated melittin in phospholipid bilayers: Effect of pH. Molecular Membrane Biology, 11(1), 79-86

Broadman, J. Bee venom (1997): the natural curative for arthritis and rheumatism. Health Resources Press, 220p.

Cerqueira, R. G. M., Ibiapina, S. I. O., Moreira, M. C., Pereira, L. V. A. C., Silva, L. C., Sá, M. C., Rocha, I. M., Reis, L. C. M., Sousa, M. A. A., Monteiro, A. L., Castro, S. A. D., Rodrigues, A. C. E., & Silva, F. L. (2021). Apitoxin as an auxiliary tool in prophylaxis of infection against SARS-CoV-2. Research, Society and Development, 10(16), e248101623803. https://doi.org/10.33448/rsd-v10i16.23803

Chang, Y.H., & Bliven, M.L. (1979). Anti-arthritic effect of bee venom. Agents Actions 9:205- 211.

Cohen, G., Natsheh, H., Sunny, Y., Bawiec, C. R., Touitou, E., Lerman, M. A., Lazarovici, P., & Lewin, P. A. (2015). Enhanced therapeutic anti-inflammatory effect of betamethasone on topical administration with low-frequency, low-intensity (20 kHz, 100 mW/cm(2)) ultrasound exposure on carrageenan-induced arthritis in a mouse model. Ultrasound in medicine & biology, 41(9), 2449–2457. https://doi.org/10.1016/j.ultrasmedbio.2015.04.009

Di Carlo F. J., & Fiore J. V. (1958),: In Zymosan Composition.Sci. 1958, 127:756-757.11. Fleiss, J.L. (1981). Statistical Methods for Rates and Proportions.2ª ed. John Wiley & Sons Inc. Nova Iorque.

El Mehdi, I., Falcão, S. I., Harandou, M., Boujraf, S., Calhelha, R. C., Ferreira, I., Anjos, O., Campos, M. G., & Vilas-Boas, M. (2021). Chemical, Cytotoxic, and Anti-Inflammatory Assessment of Honey Bee Venom from Apis mellifera intermissa. Antibiotics (Basel, Switzerland), 10(12), 1514. https://doi.org/10.3390/antibiotics10121514

F. De Biasi., S. C. Rahal., R. dos S. Volpi., M. J. Mamprim., J. L. Sequeira,, F. M. M. Bergamo. (2004). Utilização do sulfato de condroitina no tratamento de osteoartrite induzida experimentalmente em Joelho de cães. ARS VETERINARIA, Jaboticabal, SP, Vol. 20, nº 2, 219-227

Fraenkel, L., Bathon, J. M., England, B. R., St Clair, E. W., Arayssi, T., Carandang, K., Deane, K. D., Genovese, M., Huston, K. K., Kerr, G., Kremer, J., Nakamura, M. C., Russell, L. A., Singh, J. A., Smith, B. J., Sparks, J. A., Venkatachalam, S., Weinblatt, M. E., Al-Gibbawi, M., Baker, J. F., & Akl, E. A. (2021). 2021 American College of Rheumatology Guideline for the Treatment of Rheumatoid Arthritis. Arthritis & rheumatology (Hoboken, N.J.), 73(7), 1108–1123. https://doi.org/10.1002/art.41752

Gabay, C., Hasler, P., Kyburz, D., So, A., Villiger, P., von Kempis, J., & Walker, U. (2014). Biological agents in monotherapy for the treatment of rheumatoid arthritis. Swiss Med. Wkly. 4, 144, w13950.

Gegout P., Gillet, P., Terlain, B., & Netter, P.(1995): Zymosan induced arthritis in rats II. Effects of antiinflammatory drugs. Life Sci 56: 389-394, 1995

Jang, S., & Kim, K. H. (2020). Clinical Effectiveness and Adverse Events of Bee Venom Therapy: A Systematic Review of Randomized Controlled Trials. Toxins, 12(9), 558. https://doi.org/10.3390/toxins12090558

Keystone, E. C., Schorlemmer, H. U., Pope, C., & Allison, A. C.(1977). Zymosan induced arthritis: a model of chronic proliferative arthritis following activation of the alternative pathway of complement. Artritis Rheum 20: 1397-1401

Kurkó, J., Besenyei, T., Laki, J., Glant, T. T., Mikecz. K & Szekanecz, Z. (2013). Genetics of rheumatoid arthritis - a comprehensive review. Clinical reviews in allergy & immunology, 45(2), 170–179. https://doi.org/10.1007/s12016-012-8346-7

Leão G, Leite D, & Rocha L. S.(2005) Apitoxina. Unimontes Montes Claros, 7(1)

Lee, Y. M., Cho, S. N., Son, E., Song, C. H., & Kim, D. S. (2020). Apamin from bee venom suppresses inflammation in a murine model of gouty arthritis, Journal of Ethnopharmacology, 257, 112860, https://doi.org/10.1016/j.jep.2020.112860

Lee, J. D., Kim, S. Y., Kim, T. W., Lee, S. H., Yang, H. L., Lee, D. L., & Lee, Y. H. (2004). Anti-inflammatory effect of bee venom on type II collagen-induced arthritis. American Journal of Chinese Medicine, 32(3), 361-367

Lipowitz, A. J., Wong, P. L., & Stevens, J. B. (1985). Synovial membrane changes after experimental transection of the cranial cruciate ligament in dogs. American Journal of Veterinary Research, v.46, p.1166-70.

Lorenzetti, G. J., Fortenberry, B., & Busby, E.(1972). Influence of bee venom in the adjuvantinduced arthritic rat model. Res Comm Chem Pathol Pharmacol 4(2):4,339

Madzia, A., Agrawal, C., Jarit, P., Petterson, S., Plancher, K., & Ortiz, R. (2020). Sustained Acoustic Medicine Combined with A Diclofenac Ultrasound Coupling Patch for the Rapid Symptomatic Relief of Knee Osteoarthritis: Multi-Site Clinical Efficacy Study. The open orthopaedics journal, 14, 176–185. https://doi.org/10.2174/1874325002014010176

Maia, A. B. R. A., & Rocha, M. P. S.( 2002). Fracionamento e Caracterização da apitoxina. Mensagem Doce, Apacame, São Paulo.

Marshall, J. L. (1969). Periarticular osteophytes. Initiation and formation in the knee of the dog. Clinical Orthopaedics & Related Research, (62), 37-47.

Moreira, C., & Carvalho, M. A. P. (2001). Reumatologia - Diagnóstico e Tratamento. 2a ed. Rio de Janeiro (RJ): Médica e Científica, 371-89

Moreno, M., & Giralt, E. (2015). Three Valuable Peptides from Bee and Wasp Venoms for Therapeutic and Biotechnological Use: Melittin, Apamin and Mastoparan. Toxins, 7, 1126-1150; 10.3390/toxins7041126

Morsoleto, M. J. M. S., Morsoleto, F., Alves de Matos, A., Morgado, F., & Anaruma, C. (2015). A Study of the Effects of Phonophoretic Application of Hydrocortisone on the Regeneration of Injuries to Skeletal Muscle. Microscopy and Microanalysis, 21(S5), 77-78. 10.1017/S1431927615014191

Morsoleto, M. J. M. S., Reis R. R., Amstalden, E. M. I., & Bertolo, M. B. (2007). Clinical and morphologic evolution of the induced experimental artrite in Rattus novergicus albinus to wistar, Braz . J. Morphol. Sci, 24 (2), 75-81

Morsoleto, M. J. M. S., (2003). Transmissão de produtos farmacêuticos tópicos através de energia ultra-sônica na regeneração de lesões da musculatura esquelética – enfoque morfológico Unesp/RC

Nader, R. A., Mackieh, R., Wehbe, R., El Obeid, D., Sabatier, J. M., & Fajloun, Z. (2021). Beehive Products as Antibacterial Agents: A Review. Antibiotics, 10(6), 717. https://doi.org/10.3390/antibiotics10060717

Oliver S. J, & Brahn, E. (1996). Combination therapy in rheumatoid arthritis: the animal model perspective. J Rheum 23 (Supl 24): 56-60

Orr, C., Vieira-Sousa, E., Boyle, D. L., Buch, M. H., Buckley, C. D., Cañete, J. D., Catrina, A. I., Choy, E., Emery, P., Fearon, U., Filer, A., Gerlag, D., Humby, F., Isaacs, J. D., Just, S. A., Lauwerys, B. R., Le Goff, B., Manzo, A., McGarry, T., McInnes, I. B., & Tas, S. W. (2017). Synovial tissue research: a state-of-the-art review. Nature reviews. Rheumatology, 13(8), 463–475. https://doi.org/10.1038.

Pond, M. J., & Nuki, G. (1973). Experimentally-induced osteoarthritis in the dog. Annals of the Rheumatic Diseases, 32, 387-8.

Reitman, M. L. (2018). Of mice and men – environmental temperature, body temperature, and treatment of obesity. FEBS Letters,592(12), 2098-2107. https://doi.org/10.1002/1873-3468.13070

Saliba, S., Mistry, D. J., Perrin, D. H., Gieck, J., & Weltman, A. (2007). Phonophoresis and the absorption of dexamethasone in the presence of an occlusive dressing. Journal of athletic training, 42(3), 349–354

Sato, M., Nagata, K., Kuroda, S., Horiuchi, S., Nakamura, T., Karima, M., Inubushi, T., & Tanaka, E. (2014). Low-intensity pulsed ultrasound activates integrin-mediated echanotransduction pathway in synovial cells. Ann Biomed Eng. Oct;42(10):2156-63. 10.1007/s10439-014-1081-x

Shinjo, S. K., Moreira, C. (2021). Livro da Sociedade Brasileira de Reumatologia, (2a ed.), ed Manole.

Simics, M. (2005). Symptoms of honeybee venom. Apitronic services.

Silva, A. N. G., Oliveira, J. R. S., Madureira, Álvaro N. de M., Lima, W. A., Silva, A. P. S. & Lima, V. L. M. (2021). Muscle regeneration mechanisms stimulated by ultrasound therapy . Research, Society and Development, 10(12), e579101221044. https://doi.org/10.33448/rsd-v10i12.21044

Silvano, D. A., Ferreira, S. K., Frade, I. R., & Ribeiro, A. O. M. (2020). Uso da apitoxina como recurso terapêutico para a artrite reumatoide: uma revisão integrativa. NBC, 10(19), 108

Sokol, P. T., Hu, W., Yi, L., Toral, J., Chandra, M., & Ziai, M.R. (1994) Cloning of an apamin binding protein of vascular smooth muscle. Journal of Protein Chemistry, 13(1), 117-128.

Sun, L., An, S., Zhang, Z., Zhou, Y., Yu, Y., Ma, Z., Fan, X., Tang, L. & Guo, J. (2021). Molecular and Metabolic Mechanism of Low-Intensity Pulsed Ultrasound Improving Muscle Atrophy in Hindlimb Unloading Rats. International Journal of Molecular Sciences, 22(22), 12112. https://doi.org/10.3390/ijms222212112

Van den Berg, W. B. (2005) Animal models of arthritis. What have we learned? J Rheumatol Suppl. 2005 Jan;72:7-9

Wadsworth, J. D. F., Doorty, K. B., & Srong, P.N. (1994). Comparable 30-kDa apamin binding olypeptides may fulfill equivalent roles within putative subtypes of small conductance Ca-2- activated K/ channels. Journal of Biological Chemistry, v.269, n.27, p. 18053-18061

Watanabe, M., Kojima, S. & Hoso, M. (2017). Effect of low-intensity pulsed ultrasound therapy on a rat knee joint contracture model. Journal of physical therapy science, 29(9), 1567–1572. https://doi.org/10.1589/jpts.29.1567

Wehbe, R., Frangieh, J., Rima, M., El Obeid, D., Sabatier, J.-M. & Fajloun, Z. (2019). Bee Venom: Overview of Main Compounds and Bioactivities for Therapeutic Interests. Molecules, 24(16), 2997. https://doi.org/10.3390/molecules24162997

Weis, W. A., Ripari, N., LopesConte, F., da Silva Honorio, M., Alves Sartori, A., HageMatucci, R., & José Maurício,Sforcin, J. (2022). An overview about apitherapy and its clinical applications. Phytomedicine Plus. 2(2)

William, A. R. (1990) Phonophoresis: an in vivo. Evaluation using three topical anesthesic. Ultrasonic. 28:137-141. 10.1016/0041-624x(90)90075-y

Xu, X., & Nelson, J.W. (1994). One-disulfide intermediates of apamin exhibit native-like structure. Biochemistry, 33(17), 5253-5261.

Young-bae K., et al., (2001). Bee venom injection into an acupuncture point reduces arthritis associated edema and nociceptive responses. Pain. 90, (3,), 271-280

Zhang, S., Liu, Y., Ye, Y., Wang, X. R., Lin, L. T., Xiao, L. Y., Zhou, P., Shi, G. X., & Liu, C. Z. (2018). Bee venom therapy: Potential mechanisms and therapeutic applications. Toxicon, 148, 64–73. https://doi.org/10.1016/j.toxicon.2018.04.012

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Published

03/06/2022

How to Cite

MORSOLETO, F. M. da S. .; WERNECK, P. R. .; BOMFIM, F. R. C. do .; MORSOLETO, M. J. M. da S. . Ultrasound wave transports apitoxin in arthritic joint. - Experimental study. Research, Society and Development, [S. l.], v. 11, n. 7, p. e53311730386, 2022. DOI: 10.33448/rsd-v11i7.30386. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/30386. Acesso em: 14 nov. 2024.

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Health Sciences