Microbiological evaluation of stethoscopes used in the clinical routine of Veterinarians in the Metropolitan Region of Recife, Pernambuco, Brazil

Authors

DOI:

https://doi.org/10.33448/rsd-v11i8.30394

Keywords:

Contamination; Disinfection; Instrument; mecA; Microorganisms; Molecular analysis.

Abstract

We evaluated the level of contamination of stethoscopes used in the clinical routine of veterinarians. Specimens from 15 stethoscopes were cultured and analyzed before (M0) and after (M1) disinfecting the stethoscope diaphragm with alcohol at 70ºGL. At moment M0, bacterial growth was observed in 73.33% (11/15) of the stethoscopes. Seventeen strains were isolated, and the majority (41.2%, 7/17) were Bacillus spp. followed by Staphylococcus spp. (35.3%, 6/17) and Micrococcus spp. (23.5%, 4/17). In samples collected after disinfection with alcohol (M1), only Bacillus spp. were isolated in 13.3% (2/15) of the stethoscopes. Coagulase phenotypic testing of the Staphylococcus spp. revealed that all the strains were negative. Polymerase chain reaction was performed for the mecA resistance gene in coagulase-negative Staphylococcus strains. Amplification of mecA gene was observed in only 16.66% (1/6) of the samples. Stethoscopes are instruments that transfer pathogens, especially bacteria that may carry resistance genes. However, simple cleaning and disinfection measures in the veterinary medical clinic routine reduce contamination and minimize the risk of spread of resistant microorganisms to animals and the environment.

Author Biographies

Evellin Karolline Marques de Souza, Rural Federal University of Pernambuco

Department of Veterinary Medicine, Universidade Federal Rural de Pernambuco (UFRPE), Street Dom Manuel de Medeiros, s/n, Dois Irmãos, 52.171-900, Recife, PE, Brazil.

 

Raylson Pereira de Oliveira, Rural Federal University of Pernambuco

Department of Veterinary Medicine, Universidade Federal Rural de Pernambuco (UFRPE), Street Dom Manuel de Medeiros, s/n, Dois Irmãos, 52.171-900, Recife, PE, Brazil.

 

José Givanildo da Silva, Federal University of Campina Grande

Rural Health and Technology Center, Federal University of Campina Grande (UFCG), Avenue Universitária, s/n, Santa Cecília, 58.708-110, Patos, PB, Brazil.

Rinaldo Aparecido Mota, Rural Federal University of Pernambuco

Department of Veterinary Medicine, Universidade Federal Rural de Pernambuco (UFRPE), Street Dom Manuel de Medeiros, s/n, Dois Irmãos, 52.171-900, Recife, PE, Brazil.

 

José Wilton Pinheiro Junior, Rural Federal University of Pernambuco

Department of Veterinary Medicine, Universidade Federal Rural de Pernambuco (UFRPE), Street Dom Manuel de Medeiros, s/n, Dois Irmãos, 52.171-900, Recife, PE, Brazil.

Mateus de Melo Lima Waterloo, Federal Fluminense University

Laboratory of Veterinary Anatomical Pathology, Faculty of Veterinary Medicine, Fluminense Federal University (UFF), Avenue Almirante Ary Parreiras, nº 503, 24220-000, Niterói, RJ, Brazil. 

Mariana Lumack do Monte Barretto, Rural Federal University of Pernambuco

Department of Veterinary Medicine, Universidade Federal Rural de Pernambuco (UFRPE), Street Dom Manuel de Medeiros, s/n, Dois Irmãos, 52.171-900, Recife, PE, Brazil.

Andrea Alice da Fonseca Oliveira, Rural Federal University of Pernambuco

Department of Veterinary Medicine, Universidade Federal Rural de Pernambuco (UFRPE), Street Dom Manuel de Medeiros, s/n, Dois Irmãos, 52.171-900, Recife, PE, Brazil.

References

Agência Nacional de Vigilância Sanitária (ANVISA). (2012). Segurança do paciente em serviços de saúde: limpeza e desinfecção de superfícies (p. 118). Brasília, Brasil: ANVISA.

Becker, K., Skov, R. L., & Von Eiff, C. (2015). Staphylococcus, Micrococcus, and other catalase-positive cocci. In: Jorgensen, J. H., Pfaller, M. A., Carroll, K. C., Funke, G., Landry, M. L., Richter, S. S., & Warnock, D.W.(Eds.). Manual of Clinical Microbiology (11th ed., pp. 354-382). Washington, DC:ASM Press.

Dantas, A. V. S., Vieira, L. A. O., Amorim, A. V. O., Santos, M. S., Souza, E. C., Souza, L. I. O., Fidelis, T. A. A., Souza, V. P., & Lima, G. M. S. (2014). Staphylococcus aureus resistentes à meticilina isolados de estetoscópio hospitalar. Journal of the Health Sciences Institute, 32(2), 145-147.

Dutra, L. G. B., Nascimento Neto, H. B., Nedel, F. B., & Lobo, E. A. (2013). Prevalência de contaminação bacteriana em estetoscópios. Revista do Instituto Adolfo Lutz, 72(2), 155-160.

Fan, H. H., Kleven, S. H., & Jackwood, M. W. (1995). Application of polymerase chain reaction with arbitrary primers to strain identification of Mycoplasma gallisepticum. Avian Disease, 39(4), 729-735.

Ghoshal, U., Prasad, K. N., Singh, M., Tiwari, D. P., & Ayyagari, A. (2004). A Comparative Evaluation of Phenotypic and Molecular Methods for the Detection of Oxacillin Resistance in Coagulase-Negative Staphylococci. Journal of Infection and Chemotherapy, 10(2), 86-89.

Hira, V., Sluijter, M., Estevão, S., Horst-Kreft, D., Ott, A., De Groot, R., Hermans, P. W., & Kornelisse, R. F. (2007). Clinical and molecular epidemiologic characteristics of coagulase-negative staphylococcal bloodstream infections in intensive care neonates. The Pediatric Infectious Disease Journal, 26(7), 607-612.

Hirata, Y., Sata, M., Makiuchi, Y., Morikane, K., Wada, A., Okabe, N., & Tomoike, H. (2009). Comparative analysis of Micrococcus luteus isolates from blood cultures of patients with pulmonary hypertension receiving epoprostenol continuous infusion. Journal of Infection and Chemotherapy, 15(6), 424-425.

Khan, A., Aung, T. T., & Chaudhuri, D. (2019). The First Case of Native Mitral Valve Endocarditis due to Micrococcus luteus and Review of the Literature. Case Reports in Cardiology, Article ID 5907319, 3 pages.

Logan, N. A., & DeVos, P. (2015). Bacillus. In: Whitman, W. B., Rainey, F., Kämpfer, P., Trujillo, M., Chun, J., DeVos, P., Hedlund, B., & Dedysh, S. (Eds.). Bergey’s Manual of Systematics of Archaea and Bacteria(pp. 1-163). Hoboken, United States: John Wiley & Sons.

Monteiro, R. F. S., Santos, V. R. R., Ferreira, A. A. C. T., & Abreu, J. R. G. (2020) O uso indiscriminado de antimicrobianos para o desenolvimento de micro-organismos resistentes. Revista Eletrônica Acervo Saúde, v. 53, e3597.

Nakagawa, S., Taneike, I., Mimura, D., Iwakura, N., Nakayama, T., Emura, T., Kitatsuji, M., Fujimoto, A., & Yamamoto, T. (2005). Gene sequences and specific detection for Panton-Valentine leukocidin. Biochemical and Biophysical Research Communications, 328(4), 995-1002.

Oliveira, A. C., & Damasceno, Q. S. (2010). Superfícies do ambiente hospitalar como possíveis reservatórios de bactérias resistentes: uma revisão. Revista da Escola de Enfermagem da USP, 44(4), 1118-1123.

Pereira, E. P., & Cunha, M. L. R. S. (2009). Avaliação da colonização nasal por Staphylococcus spp. resistente à oxacilina em alunos de enfermagem. Jornal Brasileiro de Patologia e Medicina Laboratorial, 45(5), 361-369.

Rosa, J. O., Moura, J. P., Palos, M. A. P., Gir, E., Reis, C., Kipnis, A., Canini, S. R. M. S., Belissimo-Rodrigues, F., & Pimenta, F. C. (2009). Detecção do gene mecA em estafilococos coagulase negativa resistentes à oxacilina isolados da saliva de profissionais da enfermagem. Revistada Sociedade Brasileira de Medicina Tropical, 42(4), 398-403.

Santos, A. L., Santos, D. O., Freitas, C. C., Ferreira, B. L. A., Afonso, I. F., Rodrigues, C. R., & Castro, H. C. (2007). Staphylococcus aureus: visitando uma cepa de importância hospitalar. Jornal Brasileiro de Patologia e Medicina Laboratorial, 43(6), 413-423.

Sexton, T., Clarke, P., O’Neill, E., Dillane, T., Humphreys, H. (2006). Environmental reservoirs of methicillin-resistant Staphylococcus aureus in isolation rooms: correlation with patient isolates and implications for hospital hygiene. Journal of Hospital Infection, 62(2), 187-194.

Teixeira, A. A., Risola, B. M., Dias-Netto, H. P., Andrade, M. S., Valente, E., Nielebock, M. A. P., & Sangenis, L. H. C. (2015). Eficácia do álcool gel na desinfecção de estetoscópios contaminados por Staphylococcus aureus resistente à meticilina. Revista de Epidemiologia e Controle de Infecção, 5(4),187-190.

Teixeira, A. A., Risola, B., Dias-Netto, H., Andrade, M. S., & Valente E. (2016). Avaliação da contaminação por Staphylococcus aureus resistente a droga beta-lactâmica de estetoscópios dos profissionais de um Hospital Escola em uma cidade do interior do Rio de Janeiro. Revista Saber Digital, 9(2), 117-128.

Velázquez-Meza, M. E. (2005). Staphylococcus aureus methicillin-resistant: emergence and dissemination. Salud Pública de México, 47, 381-387.

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Published

26/06/2022

How to Cite

SOUZA, E. K. M. de .; OLIVEIRA, R. P. de .; SILVA, J. G. da .; MOTA, R. A. .; PINHEIRO JUNIOR, J. W. .; WATERLOO, M. de M. L. .; BARRETTO, M. L. do M. .; OLIVEIRA, A. A. da F. . Microbiological evaluation of stethoscopes used in the clinical routine of Veterinarians in the Metropolitan Region of Recife, Pernambuco, Brazil. Research, Society and Development, [S. l.], v. 11, n. 8, p. e44611830394, 2022. DOI: 10.33448/rsd-v11i8.30394. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/30394. Acesso em: 12 nov. 2024.

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Section

Agrarian and Biological Sciences