Secondary components in Eugenia (Myrtaceae): Extraction and Efficiency as an antimicrobial in E. francavilleana and E. paracatuana

Authors

DOI:

https://doi.org/10.33448/rsd-v11i8.30738

Keywords:

Organic extractives; Plasticizing Agents; Antimicrobial Properties; Essential oils; Myrtaceae; Leaves.

Abstract

Organic extractives (OrgExt), also known as a secondary component of plants, have attracted interest of the scientific community in recent years due to their low toxicity and remarkable antimicrobial activity. This present work has aimed to increase knowledge about OrgExt of two Myrtaceae species of Brazilian flora: Eugenia francavilleana and E. paracatuana. OrgExtr of selected species were extracted by two different organic solvents to compare their efficiency and yieldness, namely: cylohexane/ethanol and hexane.  Paired T statistical method was used to evaluate yield of extraction process for both species. Quantitative determination of the main compounds presented in structures of plant tissues was performed (Klason lignin content, α-cellulose, holocellulose and polyoses). Inorganic components presented in ash of species were determined by EDS. Among organic solvents tested for extraction of essential substances, the most efficient was hexane, being Eugenia francavilleana the species that provided the highest yield of extracted oil. OrgExt demonstrated simultaneous efficiency in substitution of glycerol (plasticizer) in preparation of thermoplastic starch films and as antimicrobial agents, delaying and reducing appearance of microorganisms in these films. This study has increased knowledge of two species whose OrgExt had never been studied before and demonstrated a potential application.

Author Biographies

Stefany Lenci Wolf, Federal University of São Carlos

Lignocellulosic Materials Laboratory, Science and Technology Center for Sustainability (CCTS), Federal University of São Carlos (UFSCar), João Leme dos Santos, km 110, 18052-780, Sorocaba, Brazil

Amanda de Sousa Martinez de Freitas, Federal University of São Paulo

Polymers and Biopolymers Technology Laboratory (TecPBio), Institute of Science and Technology (ICT), Federal University of São Paulo (UNIFESP), 12231-280 São José do Campos, SP, Brazil

Vagner Roberto Botaro, Federal University of São Carlos

Lignocellulosic Materials Laboratory, Science and Technology Center for Sustainability (CCTS), Federal University of São Carlos (UFSCar), João Leme dos Santos, km 110, 18052-780, Sorocaba, Brazil

Fiorella Fernanda Mazine Capelo, Federal University of São Carlos

Federal University of São Carlos (UFSCar), João Leme dos Santos, km 110, 18052-780, Sorocaba, Brazil.

References

ABNT NBR 14929 (2017). Determinação do teor de umidade de cavacos - Método por secagem em estufa. Associação brasileira de Normas Técnicas, 1-3.

ASTM D1103-60 (1985). Standard Test Method of test for alpha-cellulose in wood. American Society for Testing and Material International. 1-4.

ASTM D1104-56 (1978). Standard Method for Test for Holocellulose in Wood. American Society for Testing and Material. 1-2.

ASTM D1102-84 (2013). Standard Test Method for Ash in Wood. American Society for Testing and Materials. 1-2.

ASTM D1106-96 (2007). Standard Test Method for Acid Insoluble Lignin in Wood. American Society for Testing and Materials.1-2.

ASTM D1110-84 (2007). Standard Test Methods for Water Solubility of Wood. American Society for Testing and Materials International.1–2

Antônio, C., Sousa, F. De, & Sodek, L. (2002). The metabolic response of plants to oxygen deficiency. Brazilian Journal of Plant Physiology, 14(2), 83–94.

Azevedo, S. G. (2014). Caracterização quimica e atividades biológicas dos óleos essenciais das folhas de Eugenia spp. (Mirtaceae) ocorrentes na Amazônia de terra firme. Universidade Federal do Amazonas.

Bizzo, H. R., Ana Maria, C. H., & Rezende, C. M. (2009). Óleos essenciais no Brasil: aspectos gerais, desenvolvimento e perspectivas. Quimica Nova, 32(3), 588–594. https://doi.org/10.1590/S0100-40422009000300005

Botaro, V. R., & Rodrigues, J. S. (2019). A Desconstrução Dos Tecidos Vegetais E Possibilidades Atuais de produção de Novos Materiais (V. R. Botaro & J. S. Rodrigues (eds.); 1 ed). Editora Espaço Acadêmico. http://www.ppgcm.ufscar.br/arquivos/adesconstrucao_ebook16_12.pdf

Cardoso, C. A. L. (2021). Plantas do gênero Campomanesia: potenciais medicinal e nutracêutico. Editora UEMS. https://livros.uems.br/index.php/Editora/catalog/view/27/27/88

Carvalho, A. F., Silva, D. M., Silva, T. R. C., Scarcelli, E., & Manhani, M. R. (2014). Avaliação da atividade antibacteriana de extratos etanólico e de ciclohexano a partir das flores de camomila ( Matricaria chamomilla L .). Revista Brasileira de Plantas Medicinais, 16, 521–526.

Couto, R. O. do, Valgas, A. B., Bara, M. T. F., & Paula, J. R. de. (2009). Caracterização físico-química do pó das folhas de Eugenia dysenterica DC.(Myrtaceae). Revista Eletrônica de Farmácia, 6(3), 59–69.

Da Silva, A. T., & Mazine, F. F. (2016). A família Myrtaceae na Floresta Nacional de Ipanema, Iperó, São Paulo, Brasil. Rodriguésia, 67(1), 203–223. https://doi.org/10.1590/2175-7860201667110

da Silva, R. F., Carneiro, C. N., Cheila, C. B., J. V. Gomez, F., Espino, M., Boiteux, J., de los Á. Fernández, M., Silva, M. F., & de S. Dias, F. (2022). Sustainable extraction bioactive compounds procedures in medicinal plants based on the principles of green analytical chemistry: A review. Microchemical Journal, 175, 107184. https://doi.org/10.1016/j.microc.2022.107184

de Paulo Farias, D., Neri-Numa, I. A., de Araújo, F. F., & Pastore, G. M. (2020). A critical review of some fruit trees from the Myrtaceae family as promising sources for food applications with functional claims. Food Chemistry, 306, 125630. https://doi.org/10.1016/j.foodchem.2019.125630

Fernando, S., Adhikari, S., Chandrapal, C., & Murali, N. (2006). Biorefineries: Current status, challenges, and future direction. Energy and Fuels, 20(4), 1727–1737. https://doi.org/10.1021/ef060097w

Filho, I. J. da C. (2016). Separação dos principais componentes do cavaco de eucalipto, hidrólise enzimática da celulose e caracterização das frações obtidas. Universidade Federal de Pernambuco. https://repositorio.ufpe.br/handle/123456789/17763

Hsing, T. Y., de Paula, N. F., & de Paula, R. C. (2016). Características dendrométricas, químicas e densidade básica da madeira de híbridos de Eucalyptus grandis x Eucalyptus urophylla. Ciencia Florestal, 26(1), 273–283. https://doi.org/10.5902/1980509821119

Kumar, P. G., Hate, S., & Chaturvedi, A. (2009). Community based forest management and its impact on vegetation: A case study. IForest, 2(JUNE), 93–98. https://doi.org/10.3832/ifor0490-002

Liu, C., Si, C., Wang, G., Jia, H., & Ma, L. (2018). A novel and efficient process for lignin fractionation in biomass-derived glycerol-ethanol solvent system. Industrial Crops and Products, 111(August 2017), 201–211. https://doi.org/10.1016/j.indcrop.2017.10.005

Lucas, E. J., Holst, B., Sobral, M., Mazine, F. F., Nic Lughadha, E. M., Barnes Proença, C. E., Ribeiro da Costa, I., & Vasconcelos, T. N. C. (2019). A New Subtribal Classification of Tribe Myrteae (Myrtaceae). Systematic Botany, 44(3), 560–569. https://doi.org/10.1600/036364419x15620113920608

Machado, L. M. M., Nascimento, R. S., & Rosa, G. S. (2014). Estudo da extração de óleo essencial e de compostos bioativos das folhas de eucalipto (Eucalyptus citriodora). Congresso Brasileiro de Engenharia Química, 5609–5616. https://doi.org/10.5151/chemeng-cobeq2014-0754-24158-171746

Maciel, C. C., Rodrigues, J. S., De Freitas, A. S., & Ferreira, M. (2020). Study of antimicrobial property of spices in starch films: An experimental proposal. Revista Virtual de Quimica, 12(5), 1236–1243. https://doi.org/10.21577/1984-6835.20200098

Mazine, F.F.; Bünger, M.; Faria, J.E.Q.; Fernandes, T.; Giaretta, A.; Valdemarin, K.S.; Santana, K.C.; Souza, M.A.D.; Sobral, M. (2020). Eugenia in Flora do Brasil 2020. Jardim Botânico Do Rio de Janeiro. http://floradobrasil.jbrj.gov.br/reflora/floradobr

Mazine, F. F., Faria, J. E. Q., Giaretta, A., Vasconcelos, T., Forest, F., & Lucas, E. (2018). Phylogeny and biogeography of the hyper-diverse genus eugenia (Myrtaceae: Myrteae), with emphasis on E. sect. umbellatae, the most unmanageable clade. Taxon, 67(4), 752–769. https://doi.org/10.12705/674.5

Mazine, F. F., Souza, V. C., Sobral, M., Forest, F., & Lucas, E. (2014). A preliminary phylogenetic analysis of Eugenia (Myrtaceae: Myrteae), with a focus on Neotropical species. Kew Bulletin, 69(2), 9497. https://doi.org/10.1007/s12225-014-9497-x

MIGLIATO, K. F. (2005). Syzygium cumini (L.) Skeels – Jambolão: estudo farmacognóstico, otimização do processo extrativo, determinação da atividade antimicrobiana do extrato e avaliação da atividade anti-séptica de um sabonete líquido contendo o referido extrato. https://repositorio.unesp.br/handle/11449/88601

Monteiro, A. R. P. (2015). Atividade antimicrobiana de óleos essenciais. Universidade Fernando Pessoa. https://bdigital.ufp.pt/bitstream/10284/5327/1/PPG_23518.pdf

Moreira, E. L., Fazion, H., & Ribeiro, E. S. (2016). Variation of extractives three levels of forest species. 15(2), 163–172.

Nehring, P. (2016). Avaliação da capacidade antioxidante e compostos fenólicos em diferentes estádios de maturação da grumixama (Eugenia brasiliensis Lamarck). Universidade Federal de Santa Catarina. https://repositorio.ufsc.br/handle/123456789/168307

Oliveira, R. N. de, Dias, I. J. M., & Câmara, C. A. G. (2005). Estudo Comparativo de óleo essencial de Eugenia punicifolia (HBK) DC. de diferentes localidades de Pernambuco. Revista Brasileira de Farmacognosia, 15(1), 39–43.

Rodrigues, J. S., Carmo, K. P., Freitas, R. R. M., Silva, J. O., Lima, V., & Botaro, V. R. (2020). Isolation and characterization of acetosolv lignin present in sugarcane bagasse. Revista Virtual de Quimica, 12(4), 867–877. https://doi.org/10.21577/1984-6835.20200069

Rodrigues, N. M., Sandini, T. M., Perez, E., & Perez, E. (2010). Avaliação farmacognóstica de folhas de Eugenia uniflora L ., Myrtaceae ( Pitangueira ), advindas da cidade de Guarapuava. Biosaúde, 12(1), 1–13.

Sangeetha, S., Archit, R., & SathiaVelu, A. (2014). Phytochemical testing, antioxidant activity, HPTLC and FTIR analysis of antidiabetic plants Nigella sativa, Eugenia jambolana, Andrographis paniculata and Gymnema sylvestre. Research Journal of Biotechnology, 9(9), 65–72.

Sato, T. S., Medeiros, T. M. de, Hoscheid, J., & Prochnau, I. S. (2018). Proposta de formulação contendo extrato de folhas de Eugenia involucrata e análise da atividade antimicrobiana. Proposal of a formulation containing leaves extract of Eugenia involucrata. Revista Fitos, 12(1), 68–82. https://doi.org/10.5935/2446-4775.20180007

Sfredo, G. J., & Borket, C. M. (2004). Deficiências e toxidades de nutrientes em plantações de soja (1 ed).

Souza, N. D. de, Abreu, H. dos S., Elias, T. de F., Latorraca, J. V. de F., & Maeda, J. M. (2011). Dados de carbono molecular do extrato ciclohexano da madeira de Eucalyptus urophylla S. T. Blacke por RMN de 13C. Floresta e Ambiente, 18(2), 186–197. https://doi.org/10.4322/floram.2011.037

Stipp, S. R., & Casarin, V. (2010). A importância do enxofre na agricultura brasileira. Informações Agronômicas, 129(1), 14–20.

Tariq, S., Wani, S., Rasool, W., Shafi, K., Bhat, M. A., Prabhakar, A., Shalla, A. H., & Rather, M. A. (2019). A comprehensive review of the antibacterial, antifungal and antiviral potential of essential oils and their chemical constituents against drug-resistant microbial pathogens. Microbial Pathogenesis, 134, 103580. https://doi.org/10.1016/j.micpath.2019.103580

Technical Association of Pulp and Paper Industry (TAPPi T204 cm-97). (1998). Preparation of wood for chemical analysis (TAPPI T204 cm-97). TAPPI Test Methods, 2007.

Yamamoto, E. (2011). Função do cálcio na degradação da parede celular vegetal. Revista Verde de Agroecologia e Desenvolvimento Sustentável, 6(2), 8.

Published

13/06/2022

How to Cite

WOLF, S. L.; RODRIGUES, J. de S.; FREITAS, A. de S. M. de .; VALDEMARIN, K. S.; BOTARO, V. R.; CAPELO, F. F. M. Secondary components in Eugenia (Myrtaceae): Extraction and Efficiency as an antimicrobial in E. francavilleana and E. paracatuana. Research, Society and Development, [S. l.], v. 11, n. 8, p. e15211830738, 2022. DOI: 10.33448/rsd-v11i8.30738. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/30738. Acesso em: 22 nov. 2024.

Issue

Section

Agrarian and Biological Sciences