Comparison between fluoride varnish and nanohydroxyapatite in the remineralisation of active white spot lesions: a prospective clinical study

Authors

DOI:

https://doi.org/10.33448/rsd-v11i10.32552

Keywords:

Dental caries; Tooth remineralization; Hydroxyapatite; Nanoparticles.

Abstract

Objective: The initial expression of dental caries, white spot, is still frequent and requires professional care that may or may not be preventive. It is important to carry out research that evaluates different methods of remineralisation of these lesions, both in primary and permanent dentition. Thus, the aim of this study was to compare the remineralisation potential using fluoride varnish and nano-hydroxyapatite toothpaste in split-mouth design. Materials and Methods: For the research, 21 children between 6 and 12 years of age, with active non-cavitated white spots on primary and permanent teeth were selected. As a methodology, the remineralisation procedures were performed in different hemiarchs and each tooth received a different product: fluoride varnish and nano-hydroxyapatite toothpaste, both in 4 sessions, once a week. Results: Were analyzed by a calibrated examiner with no prior knowledge of the applied product; through visual criteria and measurement of white spots in their largest diameter with a digital caliper. Changes in gloss, color, texture and surface integrity were observed. The collected data received statistical treatment by means of the t-Student test, which identified the effectiveness in the reduction and remineralisation of white spots (p<0.05), by the use of fluoride varnish. Conclusion: The fluoride varnish presented statistically significant results in the treatment of active white spots. In the visual clinical evaluation, no differences were observed between fluoride varnish and nano-hydroxyapatite toothpaste. Therefore, more research is needed to offer patients viable and safe alternatives in carie prevention.

Author Biographies

Priscila Campos Zanchettin, São Paulo State University

Department of Social Dentistry and Children's Clinic of the São Paulo State University Faculty of Dentistry (UNESP), São José dos Campos, SP, Brazil.

Michelle Bianchi de Moraes, São Paulo State University

Department of Diagnosis and Surgery of the São Paulo State University Faculty of Dentistry (UNESP), São José dos Campos, SP, Brazil.

João Carlos da Rocha, São Paulo State University

Department of Social Dentistry and Children's Clinic of the São Paulo State University Faculty of Dentistry (UNESP), São José dos Campos, SP, Brazil.

References

Almeida, M. Q., Costa, O. X., Ferreira, J. M., Menezes, V. A., Leal, R. B., & Sampaio, F. C. (2011). Therapeutic potencial of Brazilian fluoride varnishes: an in vivo study. Braz Dent J, 22(3), 193-197. https://doi.org/10.1590/s0103-6440201100300003.

Cury, J. A., & Tenuta, L. M. (2009). Enamel remineralization: controlling the caries disease or treating early caries lesions? Braz Oral Res, 23 Suppl 1, 23-30. https://doi.org/10.1590/s1806- 83242009000500005.

Daas, I., Badr, S., & Osman, E. (2018). Comparison between fluoride and nano-hiddroxyapatite in remineralizing initial enamel lesion: an in vitro study. J Contemp Dent Pract , Mar;19(3), 306-312. PMID 29603704.

De Amorim, R. G., Leal, S. C., Bezerra, A. C., De Amorim, F. B., & De Toledo, O. A. (2008). Association of chlorhexidine and fluoride for plaque control and White spot lesion remineralization in primary dentition. Int J Paediatr Dent, 18(6), 446-451. https://doi.org/10.1111/i.1365- 263x.2008.00914.x.

De Carvalho, P., Bönecker, M., Tello, G., Abanto, J., Oliveira, L. B., & Braga, M. M. (2020). Inclusion of initial carie lesions in a population-based sample of Brazilian preschool children: impact on estimates and treatment needs. PloS One, 15(6), e0234122. https://doi.org/10.1371/jornal.pone.0234122.

Freitas, R. A.Jr. (2020). Nanodentistry. J Am Dent Assoc, 131(11), 1559-1566. https://doi.org/10.14219/jada.archive.2000.0084.

Grewal, N., Sharma, N., & Kaur, N. (2018). Surface remineralization potencial of nano-hidroxyapatite, sodium monofluorophosfate and amine fluoride contaning dentifrices on primary and permanent enamel surfaces: an in vitro study. J Indian Soc Pedod Prev Dent, 36(2), 158- 166. https://doi.org/10.4103/JISPPD_JISPPD_142_17.

Hellwig, E., Altenburger, M., Attin, T., Lussi, A., & Buchalla, W. (2010). Remineralization of initial carious lesions in decíduos enamel after application of dentifrices of diferente fluoride concentrations. Clin Oral Investg, 14(3), 265-269. https://doi.org/10.1007/s00784-009-0290-4.

Huang, S.B., Gao, S.S., & Yu, H.Y. (2009). Effect of nano-hydroxiapatite concentration on remineralization of initial enamel lesion in vitro. Biomed Mater, 4(3), 034104. https://doi.org/10.1088/1748-6041/4/3/034104.

Huang, S., Gao, S., Cheng, L., & Yu, H. (2010). Combined effects of nano-hidroxyapatite and Galla chinesis on remineralisation of initial enamel lesion in vitro. J Dent, 38(10), 811-819. https://doi.org/10.1016/j.dent.2010.06.013.

Huang, S., Gao, S., Cheng, L., & Yu, H. (2011). Remineralization potencial of nano-hidroxyapatite on initial enamel lesions: an in vitro study. Carie Res, 45, 460-468. https://doi.org/10.1159/00031207.

Kamath, P., Nayak, R., Kamath, S.U., & Pai D. (2017). A comparative evaluation of the remineralization potencial of three comercially avaliable remineralizing agentes on white spot lesions in primary teeth: an in vitro study. J Indian Soc Pedod Prev Dent, 35(3), 229-237. https://doi.org/10.4103/JISPPD.JISPPD_242_16.

Juntavee, A., Juntavee, N., & Sinagpulo, A. N. (2021). Nano-Hydroxyapatite Gel and Its Effects on Remineralization of Artificial Carious Lesions. International journal of dentistry, 2021, 7256056. https://doi.org/10.1155/2021/7256056.

Machiulskiene, V., Nyvad, B., Ribeiro, A.A. (2013). Diagnóstico clínico da cárie dentária. In: Duque C. Odontopediatria: uma visão contemporânea. (Cap. 14, pp. 190-203). São Paulo: Santos.

Martínez, H. R., Abdala, H .M., Treviño, E., Garza, G., Pozas, A., & Rivera, G. (2011). Aplicacíon de la nanotecnología en odontología: nano-odontología. Rev CES Odont, 24(2), 87-91.

Niwa, M., Sato, T., Li, W., Aoki, H., Aoki, H., & Daisaku ,T. (2001). Polishing and whitening properties of toothpaste containing hidroxyapatite. J Mater Sci Mater Med, 12(3), 227-281. https://doi.org/10.1023/a:1008927502523.

Oliveira, M. R. C., Oliveira, P. H. C., Oliveira, L. H. C., Horliana, A. C. R. T., César, P. F., Moura, S. K., & Bussadori, S. K. (2019). Microhardness of bovine enamel after different fluoride application protocols. Dent Mater J, Feb 8; 38(1), 61-67. https://doi.org/10.4012/dmj.2017-399.

Oliveira, M. S., Ritter, A. V., Heymann, H. O., Swift, E., Donavan, T., Brock, G., & Wright, T. (2014). Remineralization effect of CPP-ACP and fluoride for White spot lesions “in vitro”. J Dent, Dec;42(12), 1592-1602. https://doi.org/10.1016/j.jdent.2014.09.004.

Patil, N., Choudhari, S., Kulkarni, S., & Joshi, S.R. (2013). Comparative evaluation of remineralizing potencial of three agentes on artificially demineralized human enamel: an in vitro study. J Conserv Dent, 16(2), 116-120. https://doi.org/10.4103/0972-0707.108185.

Pitts, N., Baez, R., Diaz- Guallory, C., et al. (2019). Early Childhood Caries: IAPD Bangkok Declaration. Int J Paediatr Dent, 29(3), 384–386. https://doi.org/10.1111/ipd.12490.

Roveri, N., & Iafisco, M. (2010). Envolving application of biomimetic nanostructured hidroyapatite. Nanotechnol. Sci Appl, 3,107-125. https://doi.org/10.2147/NSA.S9038.

Savas, S., Kavrìk, F., & Kucukyìlmaz, E. (2016). Evaluation of the remineralization capacity of CPP- ACP containing fluoride varnish by different quantitative methods. J Appl Oral, 24(3), 198-203. https://doi.org/10.1590/1678-775720150583.

Seppä, L. (2004). Fluoride varnishes in caries prevention. Med Princ Pract, 13(6), 307-311. https://doi.org/10.1159/000080466.

Tschoppe, P., Zandim, D.L., Martus, P., & Kielbassa, A.M. (2011). Enamel and dentine remineralization by nano-hidroxyapatite toothpastes. J Dent, 39(6), 430-437. https://doi.org/10.1016/j.jdent.2011.03.008.

Wierichs, R. J., Wolf, T. G., Campus, G., & Carvalho, T. S. (2022). Efficacy of nano-hydroxyapatite on caries prevention-a systematic review and meta-analysis. Clinical oral investigations, 26(4), 3373–3381. https://doi.org/10.1007/s00784-022-04390-4.

Published

27/07/2022

How to Cite

ZANCHETTIN, P. C.; MORAES, M. B. de; ROCHA, J. C. da . Comparison between fluoride varnish and nanohydroxyapatite in the remineralisation of active white spot lesions: a prospective clinical study . Research, Society and Development, [S. l.], v. 11, n. 10, p. e186111032552, 2022. DOI: 10.33448/rsd-v11i10.32552. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/32552. Acesso em: 20 apr. 2024.

Issue

Section

Health Sciences