Fungi decontamination by gaseous ozone of fresh, dried and salted mackerel (Acanthocybium solandri)
DOI:
https://doi.org/10.33448/rsd-v9i5.3445Keywords:
Antifungal; Ozone; Fungi; Spores; Fish.Abstract
The aim of this study was to investigate the antifungal effect of ozone gas (O3) (green decontamination agent) on mackerel fish (Acanthocybium solandri) in different types of preservation (fresh, dry and salted). Samples humidity [mc / aw] parameters prior gas treatment, were of 80.7%/0.98, 55.55%/0.74 and 49.5%/0.70, respectively. Fish were contaminated with fungi genera that are able to grow on high (Fusarium) and low (Aspergillus / Penicilliun) moisture content substrates, then gas treated at 50 μmol O3 /mol, exposed during 10, 20 and 30 min, and incubated (25ºC, 7 days) to evaluate fungal inactivation. The O3 treated samples, when O3 exposed during the longest time (30 min/Day 7th) had the fungal growth totally inhibited (100%), while the others presented only reduced growth effect, i.e; 40 and 70 % for 10 and 20 min, respectively. Fusarium spores were not able to grow in any of the protein based sample studied (both, Control & Treated Group). On the other hand, Aspergillus and Penicilliun although grew on Control and some treated ones, their growth was inhibited by O3 depending on exposure time. That gas showed (under the conditions applied) to be fungi control effective for mackerel different forms of conservation.
References
Alexandre, E. M. C., Santos-Pedro, D. M.; Brandao, T. R. S.; Silva, C. L. M. (2011). Influence of aqueous ozone, blanching and combined treatment son microbial load of red bell peppers, strawberries and water cress. Journal of Food Engineering, 105(1): 277-282.
Association of Official Analytical Chemists – AOAC. (2002). Official methods of analysis of the Association of Official Analytical Chemists. 17th ed. Gaithersburg: AOAC.
Aquino, C. M.; Rollemberg, N. C.; Silva, B. A.; Runtzel, C. L.; Silva, N. C., Scussel, V. M. (2019). Different parasites in fishery products: A review. Revista Brasileira de Higiene e Sanidade Animal, 13(2): 266 – 288.
Augusto, P. E. D. (2017). Princípios de Tecnologia de Alimentos. 1ª ed. Atheneu, 424 p.
Baltazar, C., Sanches, S. A., Telles, E. O., Merusse, J. L. B., Balian, S. C. (2013). Quality parameters of salt-dried codfish stored at both refrigerated and ambient temperatures. Brazilian Journal of Food Technology, 16(3): 236-242.
Beber-Rodrigues, M., Savi, G. D., Scussel, V. M. (2015). Ozone effect on fungi proliferation and genera susceptibility of treated stored dry paddy rice (Oryza sativa L.). Journal of Food Safety, 35(1): 59-65.
Brabo, M. F., Pereira, L. F. S., Santana, J. V. M., Campelo, D. A. V., Veras, G. C. (2016). Current scenario of fish production in the world, Brazil and Pará State: emphasis on aquaculture. Acta of Fisheries and Aquatic Resources, 4(2): 50-58.
BRASIL. 2001. Ministério da Saúde. Agência Nacional de Vigilância Sanitária. Resolução RDC n. 12, de 02 de janeiro de 2001. Aprova o regulamento técnico sobre padrões microbiológicos para alimentos. Diário Oficial da República Federativa do Brasil, Brasília, DF, 10 de janeiro de 2001. Seção 1, p.45-53.
BRASIL. 2017. Ministério da Agricultura Pecuária e Abastecimento. Decreto nº 9.013, de 29 de março de 2017. Regulamenta a inspeção industrial e sanitária de produtos de origem animal, que disciplina a fiscalização e a inspeção industrial e sanitária de produtos de origem animal. Diário Oficial da República Federativa do Brasil, Brasília, 30 de março de 2017, Brasília, DF.
Chawla, A., Bell, J. W., Marlene, E. J. (2007). Optimization of Ozonated Water Treatment of Wild-Caught and Mechanically Peeled Shrimp Meat. Journal of Aquatic Food Product Technology, 16(2): 41-56.
Christ, D., Savi, G. D., Scussel, V. M. (2016). Effectiveness of Ozone Gas in Raw and Processed Food for Fungi and Mycotoxin Decontamination - A Review. Journal of Chemical, Biological and Physical Sciences, 6(2): 326-348.
DECAGON. (2016). Manual AquaLab ATE Series Manual. Retrieved March 6, 2020, from http://aqualab.decagon.com.br/educacao/aqualab-series-4te-manual/
Dhaneesh, K. V., Noushad, K. M., Ajithkumar, T. T. (2012). Nutritional evaluation of commercially important fish species of Lakshadweep Archipelago, India. PLOS ON, 7: 1-7.
Fellows, P. J. (2006). Tecnologia do processamento de alimentos: Princípios e prática. 2ª ed. Artmed, Porto Alegre, 602p.
Ferreira, M. W., Silva, V. K., Bressan, M. C., Faria, P. B., Vieira, J. O., Oda, S. H. I. (2002). Pescados processados: Maior vida de prateleira e maior valor agregado. Boletim de extensão rural. Universidade Federal de Lavras- MG.
Forsythe, S. J. (2002). Microbiologia da segurança alimentar. Artmed, Porto Alegre, 424p.
Franco, B. G. M. & Landgraf, M. (1999). Microbiologia dos alimentos. 1ª ed. Atheneu, São Paulo, 182p.
Franco, B. G. M. & Landgraf, M. (2008). Microbiologia dos alimentos. 2ª ed. Atheneu, São Paulo, 182p.
Freitas-Silva, O., Morales-Valle, H., Venancio, A. (2013). Potential of aqueous ozone to control aflatoxigenic fungi in brazil nuts. Biotechnology, 13: 1-6.
Frisvad, J. C. & Samson, R. A. (2004). Polyphasic taxonomy of Penicillium subgenus Penicillium. A guide to identification of food and air-borne terverticillate Penicillia and their mycotoxins. Studies in mycology, 49(1): 1-174.
Giordano, B. N. E., Nones, J., Scussel, V. M. (2012). Susceptibility of the in shell Brazil nut mycoflora and aflatoxin contamination to ozone gas treatment during storage. Journal of Agricultural Science, 4(8): 1-10.
Gonçalves, A. A. (2009). Ozone – an Emerging Technology for the Seafood Industry. Brazilian Archives Of Biology And Technology, 52(6): 1527-1539.
GONÇALVES A. A. (2011). Tecnologia do pescado: ciência, tecnologia, inovação e legislação. Atheneu, São Paulo, 608p.
Guzel-Seydim, Z., Greene & A. K., Seydim, A. C. (2004). Use of ozone in the food industry. Lebensmittel-Wissenschaft und-Technologie, 37: 453-460.
Hosomi, R., Yoshida, M., Fukunaga, K. (2012). Seafood Consumption and Components for Health. Global Journal of Health Science, 4(3): 72-86.
International Commission On Microbiological Specificatios For Foods (ICMSF). (2015). Microrganismos em Alimentos 8 – Utilização de Dados para Avaliação do Controle de Processo e Aceitação de Produto. Edgard Blucher, São Paulo, 522p.
Kreibich, H. H., Christ, D., Maria, G. S., Silva, J. R., Savi, G. D., Scussel, V. M. (2016). Decontamination of cocoa beans (Theobroma cacao L.) inoculated with Aspergillus flavus by Ozone gas. Journal of Chemical, Biological and Physical Sciences, 6: 560-570.
Koneman, E., Winn, J. R. W., Allen, S., Janda, W., Procop, G., Schrec-Kenberger, P. (2001). Diagnostico microbiológico: texto e atlas colorido. 5ª ed. Guanabara, Rio de Janeiro, 1465p.
Lima, E. J. V. M. O. & Sant’Ana, L. S. (2011). Water activity, moisture and salt levels in imported salted and dried fish. Brazilian Journal of Food Technology, 14(2): 125-129.
López, C. C., Serio, A., Montalvo, C., Ramirez, C., Álvarez, J. A. P., Paparella, A., Mastrocola, D., Martuscelli, M. (2017). Effect of nisin on biogenic amines and shelf life of vacuum packaged rainbow trout (Oncorhynchus mykiss) fillets. Journal of Food Science and Technology, 54: 3268–3277.
Luiz, D. B., Silva, C. D. F., Campelo, S. R., Santos, V. R. V., Lima, L. K. F., Chicrala, P. C. M. S., Iwashita, M. K. P. (2017). Evaluation of the effectiveness of ozone as a sanitizer for fish experimentally contaminated with Salmonella sp. Brazilian Journal of Food Technology, 20: 1-7.
Maciel, R. A., Rodrigues, A. M. C., Pena, R. S. (2016). Influence of the process parameters on osmotic dehydration of mapara (Hypophthalmus edentatus) fillet. Journal of Food Science and Technology, 53: 676–684.
MARTINS, F. O. 2006. Avaliação da qualidade higiênico-sanitária de preparações (sushi e sashimi) a base de pescado cru servidos em bufes na cidade de São Paulo. Disertação. Mestrado em Saúde Pública. Universidade de São Paulo – USP, São Paulo-SP. 142p.
McDonough, M. X., Campabadal, C. A., Mason, L. J., Maier, D. E., Denvir, A., Woloshuk, C. (2011). Ozone application in a modified screw conveyor to treat grain for insect pests, fungal contaminants, and mycotoxins. Journal of Stored Products Research, 47(3): 249-254.
Meronuck, R. A. (1987). The significance of fungi in cereal grains. Plant Disease, 71: 287-291.
Molina-Filho, L., Pedro, M. A. M., Telis-Romero, J., Barboza, S. H. R. (2006). Influence of temperature and concentration of the chloride sodium (NaCl) on sorption isotherms of tambaqui meat (Colossoma macroparum). Food Science and Technology, 26(2): 453-458.
Mukhopadhyaya, S., Sokoraia, K., Ukukub, D. O., Fana, X., Olanyab, M., Junejaa, V. (2019). Effects of pulsed light and sanitizer wash combination on inactivation of Escherichia coli O157:H7, microbial loads and apparent quality of spinachleaves. Food Microbiology, 82: 127–134.
Neiva, C. R. P. (2002). Valor Agregado e Qualidade do Pescado. Revista Panorama da Aqüicultura, Rio de Janeiro, p. 46-47. Retrieved March 9, 2020, from https://panoramadaaquicultura.com.br/valor-agregado-e-a-qualidade-do-pescado/
Nespolo, C. R., Oliveira, F. A., Pinto, F. S. T., Oliveira, F. C. (2015). Práticas em Tecnologia de Alimentos. 1ª ED. ARTMED, 220 P.
Nuwanthib, S. G. L. I., Madagea, S. S. K., Hewajuligea, I. G. N., Wijesekera, R. G. S. (2016). Comparative study on organoleptic, microbiological and chemical qualities of dried fish, goldstripe sardinella (Sardinella gibbosa) with low salt levels and spices. Procedia Food Science, 6: 356 – 361.
Ordónez, J. A. (2005). Tecnologia de Alimentos - alimentos de origem animal. v. 2. ARTMED, Porto Alegre, 279 P.
PEIXEBR (Associação Brasileira da Piscicultura). (2019). Anuário PEIXEBR da Piscicultura 2019. 148p.
Samson, R. A., Hong, S. B., Frisvad, J. C. (2006). Old and new concepts of species differentiation in Aspergillus. Medical Micology, 44(1): 133–148.
Santos, F. M. S., Silva, A. I. M., Vieira, C. B., Araújo, M. H., Silva, A. L. C., Cunha, M. G. C., Souza, B. W. S., Bezerra, R. S. (2017). Use of chitosan coating in increasing the shelf life of liquid smoked Nile tilapia (Oreochromis niloticus) fillet. Journal of Food Science and Technology, 54(5): 1304–1311.
Savi, G. D., Piacentini, K. C., Bittencourt, K. O., Scussel, V. M. (2014). Ozone treatment efficiency on Fusarium graminearum and deoxynivalenol degradation and its effects on whole wheat grains (Triticum aestivum L.) quality and germination. Journal of Stored Products Research, 59: 245−253.
Scussel, V. M., Savi, G. D., Klauman, T., Tonon, K. M. (2018). Micotoxinas em grãos armazenados e seus limites máximos tolerados. In: Lorini, I., Miike, L. H., Scussel, V. M, Faroni, L. R. D. Armazenagem de grãos. Jundiaí: Instituto BioGeneziz, p 759-831.
Silva S. B., Luvielmo, M. M., Geyer, M. C., Prá, I. (2011). Potencialidades do uso do ozônio no processamento de alimentos. Ciências Agrárias, 32(2): 659-682.
Silva, A. M. M. & Gonçalves, A. A. (2014). Potencialidade do uso de água ozonizada no processamento de peixes. Acta of Fisheries and Aquatic Resources, 2(1): 15-28.
SILVA, A. M. M. 2015. Efeito antimicrobiano do ozônio no processamento da tilápia do nilo, Oreochromis niloticus (LINNAEUS, 1758). (2015), Dissertação. Mestrado em Produção Animal. Universidade Federal Rural do Semi Árido, Mossoró-RN, 75p.
Silva, N., Junqueira, V. C. A., Silveira, N. F. A. (2007). Manual de métodos de análise microbiológica de alimentos. Varela, São Paulo, 536p.
Silva, S. B, Luvielmo, M. M., Geyer, M. C., Pra, I. (2011). Potencialidades do uso de ozônio no processamento de alimentos. Ciências Agrárias, 32(2): 659 – 682.
Soares, F. M. V., Vale, S. R., Junqueira, R. G., Glória, B. A. (1998). Teores de histamina e qualidade físico-química sensorial de filé de peixe congelado. Ciência e Tecnologia Alimentos, 18(4): 462-470.
Soares, C. E, Weber, A., Moecke, E., Souza, C. K., Reiter, M. G. R., Scussel, V. M. (2018). Use of Ozone Gas as a Green Control Alternative to Beetles Alphitobius diaperinus (Panzer) Infestation in Aviary Bed Utilized in the Poultry Industry. Chemical Engineering Transactions, 64: 586-594.
Statsoft. (2007). Statistica for Window - Computer programa manual. Versão 7.0. Tulsa: Statsoft Inc.
Vaz-Velho, M., Silva, M. V., Pessoa, J., Gibbs, P. A. (2006). Inactivation by ozone of Listeria innocua on salmon-trout during cold-smoke processing. Food control, 17(8): 609-616.
Vieira, R. H. S. F. (2004). Microbiologia, higiene e qualidade do pescado. 1ª ed. Varela, São Paulo, 380p.
Weber, R. W. S. & Pitt, D. (2000). Teaching techniques for mycology: 11. Riddell's slide cultures. Mycologist, 14(3): 118-120.
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