Counting of lactic bacteria in yogurts and dairy drinks in the metropolitan region of Recife-PE

Authors

DOI:

https://doi.org/10.33448/rsd-v11i15.37002

Keywords:

Lactobacillus; Streptococcus; Bifidobacterium; Probiotics; Functional drink.

Abstract

Fermented milk-based products, such as fermented dairy drinks and yogurts, have the advantage over the initial raw material (milk) in the production of metabolites in the form of organic acids that prolong their shelf life. Lactic acid bacteria (LAB) can act as probiotics, providing numerous benefits when consumed regularly. This article aimed to quantify the LAB present in yogurts and fermented dairy drinks sold in the Metropolitan Region of Recife (RMR), in Pernambuco. LAB quantification used the pour plate plating technique on MRS Agar and expressed in Colony Forming Unit per gram (CFU g-1). The pH and total acidity of the samples were also determined. None of the 13 yogurt             samples reached the minimum count of 107 CFU g-1 BAL, although the pH values ​​were below 4.5 (3.98 - 4.36). As for the 9 samples of fermented dairy drinks, only 4 samples had LAB concentration below 106 CFU g-1. Except for samples BLF3, BLF7, and BLF8, all fermented dairy drinks complied with the acidity and pH parameters. The evaluation of yoghurts and fermented dairy drinks carried out highlights the need for greater control in production so that the products fit the minimum requirements for the provision of the proposed benefits and greater transparency regarding the information of their respective functional components, promoting a conscious and honest consumption of the population.

References

A.O.A.C. (2011) – Association of Official Analytical Chemists. Official Methods of Analysis of AOAC International. 18th ed. Maryland: AOAC International.

Brasil. (2007). Ministério da Agricultura, Pecuária e Abastecimento (BR). Instrução Normativa (MAPA) Nº 46, de 23 de outubro de 2007. Adota o Regulamento Técnico de Identidade e Qualidade de Leites Fermentados, anexo à presente Instrução Normativa. Diário Oficial da União. Brasília, DF, 24 out. 2007. Seção 1(205):4-7.

Brasil. (2013). Norma Interna SDA nº 04, de 16 de dezembro de 2013. Programa de avaliação de conformidade de padrões físico-químicos e microbiológicos de produtos de origem animal comestíveis e água de abastecimento de estabelecimentos registrados e relacionados no Serviço de Inspeção Federal (SIF) e de produtos de origem animal comestíveis importados. Diário Oficial da União, Brasília, DF. 16 dez. 2013.

Brito, L. P. de et al. (2020). Lactic acid bacteria isolated from Coalho cheese from northeast Brazil in dairy production: A screening for technological application. Research, Society and Development. 9(10), e5249108457.

Farias, P. K. S. et al. (2016) Contagem de bactérias lácticas em iogurtes comerciais. Cad. Ciênc. Agrá. 8(3), 38-44.

FDA. (2003). Guidelines for industry. Early clinical trials with live biotherapeutic products: chemistry, manufacturing, and control information. Retrieved jul. 4, 2022, From, https://www.fda. gov/downloads/Biologi.../UCM292704.pdf. Acesso em: 04 jul. 2022.

Ferreira Neto, R., & Vieira A. A. S. (2021). Veracidade da rotulagem quanto a presença de amido baseado em um teste laboratorial em iogurtes nacionais comercializados no sul de Santa Catarina. Rev Inst Adolfo Lutz. São Paulo.

Freire, T. T. et al. (2021) Bactérias ácido lácticas suas características e importância: revisão. Research, Society and Development. 10(11), e513101119964.

Hanauer, D. C., & Mergen, I. (2019). Legislação brasileira para rotulagem de produtos lácteos. Brazilian Journal of Food Research, Campo Mourão. 10(1), 171-199.

Hernández A. H., Rodríguez C. C., & Vázquez J. J. G. (2020). Novidades em probióticos: evidencias y seguridad. Pediatr Integral XXIV. 3, 151-165.

Jha, V. et al. (2022). Exploration of Probiotic Potential of Lactic Acid Bacteria Isolated from Different Food Sources. American Journal of BioScience. 10(3), 118-130.

Kumar M. et al. (2022). Screening for probiotic attributes of lactic acid bacteria isolated from human milk and evaluation of their anti-diabetic potentials. Food Biotechnology, 36(3).

Mazlumi, A. et al. (2022). Probiotic potential characterization and clustering using unsupervised algorithms of lactic acid bacteria from saltwater fish samples. Scientific Reports. 12, 11952.

Oliveira, m. n. et al. (2013). manufacture of fermented lactic beverages containing probiotic cultures. Journal of food science. 67(6), 2336-2341.

Panciere B. M., & Ribeiro, L. F. (2021). Detecção e ocorrência de fraudes no leite fluido ou derivados. GETEC. 10(26), 1-17.

Rabêlo, A. C., et al. (2022). Quantificação da microbiota presente em produtos lácteos industrializados comercializados como probióticos. RECIMA21 - Revista Científica Multidisciplinar, 3(5), e351418.

Rodas. M. A. B., et al. (2001). Caracterização físico-química, histológica e viabilidade de bactérias lácticas em iogurtes com frutas. Ciênc. Tecnol. Aliment., Campinas, 21(3): 304-309.

Silva, N. et al. (2017). Manual de métodos de análise microbiológica de alimentos e água/ Neusely da Silva (et al). (5a ed.) Blucher.

Sousa, T.L.T.L. et al. (2022). Drink with probiotic potential based on water-soluble extract from cashew nuts. Ciência Rural, 52(11), e20210218.

Souza, P. G. et al. (2022). Avaliação da qualidade físico-química de bebidas lácteas sabor araçá-boi (Eugenia stipitata). Brazilian Journal of Science, 1(2), 59-64.

Tanika, M. et al. (2022). Probiotic potential of lactic acid bacteria isolated from different sources of food and milk products. Indian Journal of Agricultural Biochemistry. 35(1), 71-78.

Tripathi, M. K., & Giri, S. K. (2014). Probiotic functional foods: Survival of probiotics during processing and storage. Journal of Functional Foods. 9(1), 225–241.

Published

13/11/2022

How to Cite

SOUSA, T. L. T. L. .; SILVA, A. M. de S. .; SILVA, M. K. G. da .; LIMA, G. S. de .; VELOSO, R. R. .; SHINOHARA, N. K. S. . Counting of lactic bacteria in yogurts and dairy drinks in the metropolitan region of Recife-PE . Research, Society and Development, [S. l.], v. 11, n. 15, p. e157111537002, 2022. DOI: 10.33448/rsd-v11i15.37002. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/37002. Acesso em: 19 apr. 2024.

Issue

Section

Agrarian and Biological Sciences