Bacterial colonies found on heat-decontaminated reused N95 masks
DOI:
https://doi.org/10.33448/rsd-v12i3.40698Keywords:
COVID-19; Decontamination; Respiratory protection devices; N95 Respirators.Abstract
Objective: To analyze the decontamination by thermodisinfection of reused N95 masks. Methodology: Experimental study, with 10 N95 masks reused for 12 hours with a 60-hour rest period, for a total of 60 hours of reuse (following the orientation of the Health Agencies, the prolonged use did not exceed 8 hours, nor reuse for 120 hours) in a hospital in an area that generates aerosols. The reused masks were sown touching the inner part closest to the nose and mouth in a Chocolate Agar culture medium, and then submitted to decontamination by a thermo-disinfector at a temperature of 90°C for 1 hour. The plates were incubated in the biological oven at 36°C for 48 hours. After seeding, macroscopic characterization of the colonies and Gram staining was performed. Results: After the decontamination process, there was no development of microorganisms, when compared to the amount of colonies before decontamination where 2 to 14 colonies ranging from gram+ cocci to gram- Bacillus were detected. Conclusion: After thermal disinfection of N95 masks, there was an absence of microorganisms at the decontamination level; however, the masks were damaged and could not be reused. It is important to develop new studies that allow both the decontamination of N95 masks and their reuse while maintaining the filter barrier.
References
Aguiar, B. F., Lind, J., Netto, H. P., Ramirez, Y., Ramos, M. P., & Rocha, J. L. L. (2020). Reprocessing of N95 masks or equivalent: a narrative review. Journal of Infection Control, 9(2), 78–85.
ANVISA. (2004). Descrição dos meios de cultura empregados nos exames microbiológicos, Módulo IV. Agência Nacional de Vigilância Sanitária (ANVISA). http://anvisa.gov.br/servicosaude/manuais/microbiologia/mod_4_2004.pdf .
Cadnum, J. L., Li, D. F., Redmond, S. N., Jonh, A. R., Pearlmutter, B., & Donksey, C. J. (2020). Eficácia da luz ultravioleta-C e um gabinete de desinfecção de alto nível para descontaminação de respiradores N95. Imunopatógeno. 2020;5(1):52-67. 10.20411/pai.v5i1.372. eCollection 2020.
Centers for Disease Control and Prevention (2020). Implementando a reutilização do respirador de peça facial filtrante (FFR), incluindo a reutilização após a descontaminação, quando houver escassez conhecida de respiradores N95. https://blogs.cdc.gov/niosh-science-blog/2020/04/15/n95-preparado/.
Centers for Disease Control and Prevention (2020). Orientação para uso prolongado e reutilização limitada de respiradores N95. p. 1, 3. https://www.cdc.gov/coronavirus/2019-ncov/hcp/non-us-settings/outpatient-pt.html.
Coelho, W. E., Moraes, M. A., Bonsonato, F., Pedreira, M., Perfeito, J. A., Gabrielloni, M. C., Peterlini, M. A., Santos, M. V. L. D., & Taminato, M. (2021). Eficácia e segurança de método de descontaminação de máscaras n95/pff2. Revista Brasileira de Doenças Infecciosas, 25, 101064.
Kumar A, Kasloff, S. B., Cutts, T., Leung, A., Sharma, N., Vasquez-Grande, G., & Krishnan, J. (2021). Standard hospital blanket warming cabinets can be utilized for complete moist heat SARS-CoV2 inactivation of contaminated N95 masks for re-use. Scientifc Reports. 2021. 11:18316.
Kumkrong, P., Scoles, L., Brunet, Y., Baker, S., Mercier, P. H. J., & Poirier, D. (2021). Evaluation of hydrogen peroxide and ozone residue levels on N95 masks following chemical decontamination. Journal of Hospital Infection. 111 (2021) 117e124.
Maniezzo, A., Alves, C., Rossi, E., Neves, L., Ancristhian, P., & Hattori, S. (2020). Evidências sobre descontaminação e reutilização de máscaras N95. Boletim Técnico 007. Universidade Federal De Mato Grosso, Brasil. Recuperado de: acesse.one/xR1dZ
Moreira, J. L. B., Carvalho, C. B. M., &Frota, C. C. (2015). Visualização bacteriana e colorações. Fortaleza: Imprensa Universitária, 2015. 68p. https://repositorio.ufc.br/ri/bitstream/riufc/16672/1/2015_liv_jlbmoreira.pdf.
Neves, G. B. C., Bezerra, L. C. S., Portugal, W. M., Lundreger, F., Paiva, J. C. S., & Pereira, E. B. F. (2021). Proteção respiratória no centro de material e esterilização em tempos de covid-19. 96. International Journal of Development Research, 11(10), 50994-50997
Neves, G. B. C., Paiva, A. C., Viturino, M. G. S. C., Santos, J. L. S., Ferreira, D. H. S., & Silva, M. C. L. B. (2022). Microorganismos de máscaras N95 reutilizadas por 36h e depois de descontaminadas por calor o que aprendemos com a pandemia COVID-19. Research, Society and Development, 11(8), e17311830230, 2022
Ogbuoji, E. A., Zaky, A. M., & Escobar, I. C. (2021). Pesquisa e Desenvolvimento Avançados de Máscaras Faciais e Respiradores Pré e Pós-Coronavírus 2019 (COVID-19) Pandemia: Uma Revisão Crítica. Polímeros, 13(12), 1998.
Oliveira, A. C., & Lucas, T. C. (2021). É possível a descontaminação de máscaras N95 em tempos de pandemia? revisão integrativa da literatura. Revista Gaúcha de Enfermagem. 2021;42(esp):e20200146.
Pascoe, M. J., Probertson, A., Crayford, A., Durand, E., Steer, J., Castelli, A., Wesgate, R., Evans, S. L., Porch, A., & Maillard, J. Y. (2020). Dry heat and microwave-generated steam protocols for the rapid decontamination of respiratory personal protective equipment in response to COVID-19-related shortages. J Hosp Infect. 106(1):10-9.
Rupnik, B., Kramberger, T., & Marcen, B. (2017). Simulation of the hospital sterilization process. international committee. Technical Gazette. 26, 5(2019), 1486-1491.
World Health Organization (2016). Decontamination and reprocessing of medical devices for health-care facilities. Geneva: 2016. https://www.who.int/publications/i/item/9789241549851.
Yuen, J. G., et al. (2022). Dry heat sterilization as a method to recycle N95 respirator masks: The importance of fit. Plos One. https://doi.org/10.1371/journal.pone.0257963 January 5, 2022.
Zha, M., Alsarraj, J., Bunch, B., & Venzon, D. (2022) Impact on the fitness of N95 masks with extended use/limited reuse and dry heat decontamination. J Investig Med. 70:99–103.10.1136/jim-2021-001908.
Zulauf, K. E., Green, A.B., Nguyen Ba, A. N., Jagdish, T., Reif, D., Seeley, R., Dale, A., & Kirby, J. E. (2020). Microwave-Generated Steam Decontamination of N95 Respirators Utilizing Universally Accessible Materials. American Society for Microbiology. 25;11(3):e00997-20.
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Copyright (c) 2023 Giselda Bezerra Correia Neves; Alicy Gabryelle Silva de Castro; Rayane Maria da Silva; Thifanes Kelly de Queiroz ; José Luís Silva dos Santos; Mariza da Silva Matias; Edson Wanderley da Silva; Vilma Ribeiro de Lima; Miller da Costa Lima Batista e Silva; Wanuska Munique Portugal
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