Enzymatic extract Lentinus tigrinus and Trametes villosa fungi obtained by semi solid fermentation by processing agroindustrial residues for animal feeding

Authors

DOI:

https://doi.org/10.33448/rsd-v13i6.45329

Keywords:

Basidiomicetos; Ruminant; Lignin; Forage; Cellulose.

Abstract

Ligninolytic enzymes from differents Basidiomycete fungi on different substrates demonstrate microorganisms biotechnological potential on agro-industrial byproducts. The aim of this study was to evaluate the use of enzymatic extracts of Lentinus tigrinus and Trametes villosa produced by semi-solid fermentation (SSF) for processing sisal coproduct and sugarcane bagasse. The strains were evaluated for RBBR test to confirm enzymes production and subsequently subcultured for 7 days until visible growth of the mycelium. For the SSF, 48 Erlenmeyers distributed in different treatments were used. The enzymatic extract was obtained and added in BC and CDS for further Bromatological analysis. The cultivation methods used were satisfactory in terms growth fungal and ligninase production from RBBR test. The fungi showed good potential in agro-industrial by-products lignocellulosic reduction. The enzyme synthesis behavior of the fungi was different, demonstrating to be in the initial days for Lentinus tigrinus and at 14 days for Trametes villosa.

References

Andlar, M., Rezić, T., Marđetko, N., Kracher, D., Ludwig, R., & Šantek, B. (2018). Lignocellulose degradation: An overview of fungi and fungal enzymes involved in lignocellulose degradation. Engineering in Life Sciences, 18(11), 768-778.

Alves, J. R., Souza, O. D., Podlech, P. A. S., Giachini, A. J., & Oliveira, V. L. D. (2001). Efeito de inoculante ectomicorrízico produzido por fermentação semi-sólida sobre o crescimento de Eucalyptus dunnii Maiden. Pesquisa Agropecuária Brasileira, 36, 307-313.

Basso, T. P., Gallo, C. R., & Basso, L. C. (2010). Atividade celulolítica de fungos isolados de bagaço de cana-de-açúcar e madeira em decomposição. Pesquisa agropecuária brasileira, 45, 1282-1289.

Brandão, L. G. N., Pereira, L. G. R., Azevêdo, J. A. G., Santos, R. D., Aragão, A. S. L., Voltolini, T. V., & Brandão, W. N. (2011). Valor nutricional de componentes da planta e dos coprodutos da Agave sisalana para alimentação de ruminantes. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 63, 1493-1501.

Brandão, L. G. N., Pereira, L. G. R., de Azevêdo, J. A. G., dos Santos, R. D., de Araújo, G. G. L., Dórea, J. R. R., & Neves, A. L. A. (2013). Efeito de aditivos na composição bromatológica e qualidade de silagens de coproduto do desfibramento do sisal. Semina: Ciências Agrárias, 34(6), 2991-2999.

Brasil. Confederação da Agricultura e Pecuária do Brasil (CNA e Centro de Estudos Avançados em Economia Aplicada (CEPEA/USP). Disponível em https://www.cnabrasil.org.br/cna/panorama-do-agro. Acesso em 20/03/2022

Carneiro, R. T. D. O., Lopes, M. A., Silva, M. L. C., Santos, V. D. S., Souza, V. B. D., Sousa, A. O. D., & Góes-Neto, A. (2017). Trametes villosa lignin peroxidase (TvLiP): genetic and molecular characterization. Journal of microbiology and biotechnology, 27(1), 179-188.

Deswal, D., Gupta, R., Nandal, P., & Kuhad, R. C. (2014). Fungal pretreatment improves amenability of lignocellulosic material for its saccharification to sugars. Carbohydrate polymers, 99, 264-269.

Ghazanfar, M., Irfan, M., Nadeem, M., & Syed, Q. (2019). Role of bioprocess parameters to improve cellulase production: part I. In New and Future Developments in Microbial Biotechnology and Bioengineering (pp. 63-76). Elsevier.

Goldbeck, R., Andrade, C. C. P., Ramos, M. M., Pereira, G. A. G., & Maugeri, F. F. (2013). Identification and characterization of cellulases produced by Acremonium strictum isolated from Brazilian biome. Int. Res. J. Microbiol, 4(6), 135-146.

Grabber, J. H. (2005). How do lignin composition, structure, and cross‐linking affect degradability? A review of cell wall model studies. Crop science, 45(3), 820-831.

Isikhuemhen, O. S., Mikiashvili, N. A., Adenipekun, C. O., Ohimain, E. I., & Shahbazi, G. (2012). The tropical white rot fungus, Lentinus squarrosulus Mont.: lignocellulolytic enzymes activities and sugar release from cornstalks under solid state fermentation. World Journal of Microbiology and Biotechnology, 28(5), 1961-1966.

Jaramillo, P. M., Gomes, H. A., Monclaro, A. V., Silva, C. O., & Filho, E. X. (2015). Lignocellulose‐degrading enzymes: An overview of the global market. Fungal biomolecules: sources, applications and recent developments, 6.

Jayasekara, S., & Ratnayake, R. (2019). Microbial cellulases: an overview and applications. Cellulose, 22.

Jung, H. G., Valdez, F. R., Abad, A. R., Blanchette, R. A., & Hatfield, R. D. (1992). Effect of white rot basidiomycetes on chemical composition and in vitro digestibility of oat straw and alfalfa stems. Journal of animal science, 70(6), 1928-1935.

Kamra, D. N., & Zadrazil, F. (1988). Microbiological improvement of lignocellulosics in animal feed production: a review. Treatment of lignocellulosics with white-rot fungi. Essex, UK: Elsevier, 56-63.

Karp, S. G., Woiciechowski, A. L., Soccol, V. T., & Soccol, C. R. (2013). Pretreatment strategies for delignification of sugarcane bagasse: a review. Brazilian archives of biology and technology, 56(4), 679-689.

Kotsampasi, Β., Christodoulou, C., Tsiplakou, E., Mavrommatis, A., Mitsiopoulou, C., Karaiskou, C., ... & Zervas, G. (2017). Effects of dietary pomegranate pulp silage supplementation on milk yield and composition, milk fatty acid profile and blood plasma antioxidant status of lactating dairy cows. Animal Feed Science and Technology, 234, 228-236.

Li, X., Pang, Y., & Zhang, R. (2001). Compositional changes of cottonseed hull substrate during P. ostreatus growth and the effects on the feeding value of the spent substrate. Bioresource Technology, 80(2), 157-161.

Kundu, S. S., Mojumdar, A. B., Singh, K. K., & Das, M. M. (2005). Improvement of poor quality roughages. Roughage Processing Technology, Satish serial publishing house, Delhi, India, 193-209.

Li, L., Li, X. Z., Tang, W. Z., Zhao, J., & Qu, Y. B. (2008). Screening of a fungus capable of powerful and selective delignification on wheat straw. Letters in Applied Microbiology, 47(5), 415-420.

Yang, L., Deng, Y., Wang, X., Zhang, W., Shi, X., Chen, X., & Zhang, F. (2021). Global direct nitrous oxide emissions from the bioenergy crop sugarcane (Saccharum spp. inter-specific hybrids). Science of the Total Environment, 752, 141795.

Marques, G. L., Silva, T. P., Lessa, O. A., de Brito, A. R., Reis, N. S., Fernandes, A. D. A., & Franco, M. (2019). Production of xylanase and endoglucanase by solid-state fermentation of jackfruit residue. Revista Mexicana de Ingeniería Química, 18(2), 673-680.

Missio, R. L. (2016). Tratamento do bagaço de cana-de-açúcar para alimentação de ruminantes. Archivos de zootecnia, 65(250), 267-278.

Mahesh, M. S., & Mohini, M. (2013). Biological treatment of crop residues for ruminant feeding: A review. African Journal of Biotechnology, 12(27).

Oliveira, R. L., Leão, A. G., Abreu, L. L., Teixeira, S., & Silva, T. M. (2013). Alimentos alternativos na dieta de ruminantes. Revista Científica de Produção Animal, 15(2), 141-160.

Galaviz-Rodriguez, J. R., Cruz-Monterrosa, R. G., & Vargas-López, S. (2010). Influence of Pleurotus ostreatus spent corn straw on performance and carcass characteristics of feedlot Pelibuey lambs. Indian J Anim Sci, 80(8), 754-757.

Rosa, M. F., Souza Filho, M. S. M., Figueiredo, M. C. B., Morais, J. P. S., Santaella, S. T., & Leitão, R. C. (2011). Valorização de resíduos da agroindústria. II Simpósio internacional sobre gerenciamento de resíduos agropecuários e agroindustriais–II SIGERA, 1(2011), 98-105.

dos Santos, R. D., Neves, A., Pereira, L., De Araujo, G. G. L., Voltolini, T., Costa, C., & De Oliveira, G. F. (2013). Coprodutos do desfibramento do sisal como alternativa na alimentação de ruminantes. Embrapa Semiárido-Circular Técnica (INFOTECA-E).

Silva, S. P. D., Rodrigues, M. T., Vieira, R. A. M., & Silva, M. M. C. D. (2013). In vitro degradation kinetics of protein and carbohydrate fractions of selected tropical forages. Biosci. j.(Online), 1300-1310.

Silva, D., & Queiroz, A. D. (1981). Análise de alimentos:(métodos químicos e biológicos) (p. 166). UFV, Impr. Univ..

Silva, O. D., & Beltrão, N. D. M. (1999). O agronegócio do sisal no Brasil. Embrapa, Brasília.

Silva, M. L. C., de Souza, V. B., da Silva Santos, V., Kamida, H. M., de Vasconcellos-Neto, J. R. T., Góes-Neto, A., & Koblitz, M. G. B. (2014). Production of manganese peroxidase by Trametes villosa on unexpensive substrate and its application in the removal of lignin from agricultural wastes. Advances in Bioscience and Biotechnology, 5(14), 1067.

Zavarzina, A. G., Lisov, A. V., & Leontievsky, A. A. (2018). The Role of Ligninolytic Enzymes Laccase and a Versatile Peroxidase of the White‐Rot Fungus Lentinus tigrinus in Biotransformation of Soil Humic Matter: Comparative In Vivo Study. Journal of Geophysical Research: Biogeosciences, 123(9), 2727-2742.

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Published

02/06/2024

How to Cite

BENEVIDES, R. G. .; BRANDÃO, L. G. N.; SANTOS, M. B. dos . Enzymatic extract Lentinus tigrinus and Trametes villosa fungi obtained by semi solid fermentation by processing agroindustrial residues for animal feeding. Research, Society and Development, [S. l.], v. 13, n. 6, p. e0913645329, 2024. DOI: 10.33448/rsd-v13i6.45329. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/45329. Acesso em: 30 jun. 2024.

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Section

Agrarian and Biological Sciences