Evaluation of the antifungal potential of Cymbopogon citratus essential oil in the control of the fungus Aspergillus brasiliensis
DOI:
https://doi.org/10.33448/rsd-v9i7.4697Keywords:
Lemongrass; Stored grains; Natural fungicide.Abstract
The objective of this work was to evaluate the antifungal activity of the essential oil of Cymbopogon citratus in the control of Aspergillus brasiliensis in analysis in vitro and in stored corn kernels. Two in vitro tests were carried out. One of them, by diffusion in agar and the other by microdilution in wells. The concentrations used for the agar diffusion assay were 0.2; 0.4; 0.6; 0.8 and 1.0 µL mL-1. The concentrations used for the microdilution test were: 0.1; 0.2; 0.3; 0.4; 0.5; 0.6; 0.7; 0.8; 0.9; 1.0 and 1.2 µL mL-1. The mycelial growth was evaluated over time, using the Scott-Knott test at 5% significance. From the results obtained, it was verified that the application of the essential oil of Cymbopogon citratus interferes significantly in the fungal development of the species Aspergillus brasiliensis. The microdilution test in wells was used in a qualitative way, indicating absence of growth in doses from 0.8 µL mL-1. For the corn grain test, the essential oil dosages of 0.5; 0.8 and 1.0 µL mL-1 and were stored for 42 days. There was no significant difference in the different doses of essential oil over the storage period for this assay.
References
Abarca, M. L., Accensi, F., Cano, J., & Cabañes, F. J. (2004). Taxonomy and significance of black aspergilli. Antonie Van Leeuwenhoek, 86, 33–49.
Abdulazeez, M. A., Abdullahi A. S., & James, B. D. (2016). Lemongrass (Cymbopogon spp.) Oils. In: Preedy, V.R. (Ed.). Essential oils in food preservation, flavor and safety. Cambridge: Academic Press. p.509-516.
Ajayi, E. O., Sadimenko, A. P., & Afolayan, A. J. (2016). Data showing chemical compositions of the essential oils of the leaves of Cymbopogon citratus obtained by varying pH of the extraction medium. Data Brief, 8, 599-604.
Akhila, A. (2009). Essential oil-bearing plants: The genus Cymbopogon. Boca Raton: Boca Raton:CRC Press Taylor & Francis Group. 262 p.
Aoudou, Y., Ngoune Léopold, T., Dongmo Pierre Michel, J., & Carl Moses, M. (2012). Inhibition of fungal development in maize grains under storage condition by essential oils. International Journal of Biosciences, 2(6), 41-48.
Avoseh, O., Oyedeji, O., Rungqu, P., Nkeh-Chungag, B., & Oyedeji, A. (2015). Cymbopogon species; ethnopharmacology, phytochemistry and the pharmacological importance. Molecules, 20(5), 7438-7453.
Bayala, B., Bassole, I. H., Maqdasy, S., Baron, S., Simpore, J., & Lobaccaro, J. M. A. (2018). Cymbopogon citratus and Cymbopogon giganteus essential oils have cytotoxic effects on tumor cell cultures. Identification of citral as a new putative anti-proliferative molecule. Biochimie, 153, 62-170.
Bomfim, N. S., Nakassugi, L. P., Oliveira, J. F. P., Kohiyama, C. Y., Mossini, S. A. G., Grespan, R., Nerilo, S. B., Mallmann, C. A., Abreu Filho, B. A., & Machinski Jr., M. (2015). Antifungal activity and inhibition of fumonisin production by Rosmarinus officinalis L. essential oil in Fusarium verticillioides (Sacc.) Nirenberg. Food chemistry, 166, 330-336.
Boudine, L., Louaste, B., Eloutassi, N., Chami, N., Chami, F., & Remmal, A. (2016). Antifungal activity of oregano essential oil and thymol against some fungi isolated from corn grains. International Journal of Innovation and Applied Studies, 17(4), 1120-1124.
Boukhatem, M. N., Ferhat, M. A., Kameli, A., Saidi, F., & Kebir, H. T. (2014). Lemon grass (Cymbopogon citratus) essential oil as a potent anti-inflammatory and antifungal drugs. Libyan Journal of Medicine, 9(1), 25431.
Božik, M., Nový, P., & Klouček, P. (2017). Susceptibility of postharvest pathogens to essential oils. Scientia agriculturae bohemica, 48(3), 103-111.
Brasil. (2010). Farmacopeia Brasileira. 5. ed. Brasília: Anvisa, 2010. 904 p.
Brasil. (2011). Formulário de Fitoterápicos da Farmacopéia Brasileira. Brasília: Anvisa. 126 p.
Cherrat, L., Espina, L., Bakkali, M., Pagán, R., & Laglaoui, A. (2014). Chemical composition, antioxidant and antimicrobial properties of Mentha pulegium, Lavandula stoechas and Satureja calamintha Scheele essential oils and an evaluation of their bactericidal effect in combined processes. Innovative Food Science & Emerging Technologies, 22, 221-229.
Choi, H. S. (2003). Character impact odorants of citrus hallagong [(C.unshiu Marcov x C. sinensis Osbeck) x C. reticulate Blanco] cold-pressed pell oil. Journal of Agricultural Food Chemistry, 51:2687-2692.
Dellavalle, P. D., Cabrera, A., Alem, D., Larrañaga, P., Ferreira, F., & Rizza, M. D. (2011). Antifungal activity of medicinal plant extracts against phytopathogenic fungus Alternaria spp. Chilean Journal of Agricultural Research, 71(2), 231-239.
Ebani, V. V., Najar, B., Bertelloni, F., Pistelli, L., Mancianti, F., & Nardoni, S. (2018). Chemical composition and in vitro antimicrobial efficacy of sixteen essential oils against Escherichia coli and Aspergillus fumigatus isolated from poultry. Veterinary sciences, 5(3), 62.
Ekpenyong, C. E., & Akpan, E. E. (2017). Use of Cymbopogon citratus essential oil in food preservation: Recent advances and future perspectives. Critical reviews in food science and nutrition, 57(12), 2541-2559,
Erland, L. A. E., Rheault, M. R., & Mahmoud, S. S. (2015). Insecticidal and oviposition deterrent effects of essential oils and their constituents against the invasive pest Drosophila suzukii (Matsumura) (Diptera: Drosophilidae). Crop Protection, 78, 20-26.
Farzaneh, M., Kiani, H., Sharifi, R., Reisi, M., & Hadian, J. (2015). Chemical composition and antifungal effects of three species of Satureja (S. hortensis, S. spicigera, and S. khuzistanica) essential oils on the main pathogens of strawberry fruit. Postharvest Biology and Technology, 109, 145-151.
Ferreira, D. F. (2014). Sisvar: a Guide for its Bootstrap procedures in multiple comparisons. Ciência e Agrotecnologia, 38(2), 109-112.
Fisher, M. C., Hawkins, N. J., Sanglard, D., & Gurr, S. J. (2018). Worldwide emergence of resistance to antifungal drugs challenges human health and food security. Science, 360(6390), 739-742.
Gams, W., Christensen, M., Onions, A. H., Pitt, J. I., & Samson, R. A. (1986). Infrageneric taxa of Aspergillus. In: Samson, R. A.; Pitt, J. I. Advances in Penicillium and Aspergillus Systematics. New York: Plenum Press; p.55–61.
Janatova, A., Bernardos, A., Smid, J., Frankova, A., Lhotka, M., Kourimská, L., ... & Kloucek, P. (2015). Long-term antifungal activity of volatile essential oil components released from mesoporous silica materials. Industrial Crops and Products, 67, 216-220.
Knaak N., & Fiuza L. M. (2010). Potencial dos óleos essenciais de plantas no controle de insetos e microrganismos. Neotropical Biology and Conservation, 5(2), 120-132.
Kozakiewicz, Z. (1989). Aspergillus species on stored products. Mycological Papers, 161, 1–188.
Kumar, P., Mishra, S., Kumar, A., Kumar, S., & Prasad, C. S. (2017). In vivo and in vitro control activity of plant essential oils against three strains of Aspergillus niger. Environmental Science and Pollution Research, 24(27), 21948-21959.
Lanças, F. M. (1993). Cromatografia em fase gasosa. São Paulo: Editora Acta. 240p.
Lee, L. T. (2017). Avaliação do potencial fungicida do óleo essencial de capim-limão (Cymbopogon flexuosus) no controle dos fungos Aspergillus brasiliensis e Aspergillus flavus na conservação de grãos de trigo (Triticum aestivum). M.Sc. Thesis, Fluminense Federal
Lopez-Romero, J. C., González-Ríos, H., Borges, A., & Simões, M. (2015). Antibacterial effects and mode of action of selected essential oils components against Escherichia coli and Staphylococcus aureus. Evidence-Based Complementary and Alternative Medicine, 2015(1), 1-9.
Luz, S. R., Pazdiora, P. C., Dallagnol, L. J., Dors, G. C., & Chaves, F. C. (2017). Mycotoxin and fungicide residues in wheat grains from fungicide-treated plants measured by a validated LC-MS method. Food chemistry, 220, 510-516.
Machado, R. M. A., Dias, V. M., de Souza, C. L. M., da Silva, L. B., & Freire, M. D. G. M. (2013). Avaliação de óleos essenciais sobre o crescimento in vitro do fungo Colletotrichum gloeosporioides. Perspectiva Online Ciências Biológicas e da Saúde, 8(3), 64-75.
Mosquera, T., Noriega, P., Cornejo, J., & Pardo, M. L. (2016). Biological activity of Cymbopogon citratus (DC) Stapf and its potential cosmetic activities. International Journal of Phytocosmetics and Natural Ingredients, 3(7), 1-7.
Negrelle, R. R. B., & Gomes, E. C. (2007). Cymbopogon citratus (DC.) Stapf: chemical composition and biological activities. Revista Brasileira de Plantas Medicinais, 9(1), 80-92.
Pandey, A. K., Rai, M. K., & Acharya, D. (2003). Chemical composition and antimycotic activity of the essential oils of corn mint (Mentha arvensis) and lemon grass (Cymbopogon flexuosus) against human pathogenic fungi. Pharmaceutical Biology, 41, 421-425.
Pavela, R. (2015). Essential oils for the development of eco-friendly mosquito larvicides: a review. Industrial crops and products, 76, 174-187.
Prakash, B., Kedia, A., Mishra, P. K., & Dubey, N. K. (2015). Plant essential oils as food preservatives to control moulds, mycotoxin contamination and oxidative deterioration of agri-food commodities–Potentials and challenges. Food Control, 47, 381-391.
Rath, C. C., & Patnaik, A. (2018). In-vitro antimycotic activity of selected essential oils and fungicides against Aspergillus niger and Fusarium oxysporum. Journal of Experimental Biology and Agricultural Sciences, 6(3), 490-497.
Ríos, J. L. (2016). Essential oils: What they are and how the terms are used and defined. In: Preedy, V. R. (Ed.). Essential Oils in Food Preservation, Flavor and Safety. Cambridge: Academic Press. p.3-10.
Samson, R. A., Houbraken, J. A. M. P., Kuijpers, A. F., Frank, J. M., & Frisvad, J. C. (2004).
New ochratoxin or sclerotium producing species in Aspergillus section Nigri. Studies in Mycology, 50, 45–61.
Sharma, A., Rajendran, S., Srivastava, A., Sharma, S., & Kundu, B. (2017). Antifungal activities of selected essential oils against Fusarium oxysporum f. sp. lycopersici 1322, with emphasis on Syzygium aromaticum essential oil. Journal of bioscience and bioengineering, 123(3), 308-313.
Silva, I. P., Melo, M. M., & Soto-Blanco B. (2016). Efeitos tóxicos dos praguicidas para abelhas. Revista Brasileira de Higiene e Sanidade Animal, 10(1):142–157.
Tatsadjieu, N. L., Dongmo, P. J., Ngassoum, M. B., Etoa, F. X., & Mbofung, C. M. F. (2009). Investigations on the essential oil of Lippia rugosa from Cameroon for its potential use as antifungal agent against Aspergillus flavus Link ex. Fries. Food control, 20(2), 161-166.
Tournas, V. H., & Niazi, N. S. (2018). Potentially toxigenic fungi from selected grains and grain products. Journal of Food Safety, 38(1), e12422.
Üstüner, T., Kordali, S., & Bozhüyük, A. U. (2018). Herbicidal and Fungicidal Effects of Cuminum cyminum, Mentha longifolia and Allium sativum Essential Oils on Some Weeds and Fung. Records of Natural Products, 12(6), 619.
Zhang, H., Chen, F., Wang, X., & Yao, H. Y. (2006). Evaluation of antioxidant activity of parsley (Petroselinum crispum) essential oil and identification of its antioxidant constituents. Food Research International, 39(8), 833-839.
Downloads
Published
How to Cite
Issue
Section
License
Authors who publish with this journal agree to the following terms:
1) Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
2) Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
3) Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.
