Total phenolics and antioxidant activity of the extract obtained from the fungus Penicillium purpurogenum from polluted marine environment of Maranhão

Authors

DOI:

https://doi.org/10.33448/rsd-v9i8.6205

Keywords:

Penicillium; Antioxidant; Extract.

Abstract

This article aims to evaluate the total phenolics and antioxidant activity of the prepared extract of the fungus Penicillium purpurogenum, obtained from a polluted marine environment of São Luís-MA. The research was carried out at Laguna da Jansen located in the Municipality of São Luís, Maranhão, Brazil. Four sediment collections were performed from May to September 2017, in the morning period. The samples were obtained from a depth of up to 73.5 cm with the aid of a metal dredger. The identification of the fungus followed methodologies of isolation and subsequent preparation of the extracts with evaluation of total phenolics by the Folin-Ciocalteu method and antioxidant activity by the DPPH radical sequestration method. Identification confirmed the presence of  Penicillium purpurogenum. The extracts of  P. purpurogenum  varied in the quantification of total phenolics of 791.00 mg EAT g-1  for FR1 extract and 650.82 mg EAT g-1 for FR2. In antioxidant activity, wecould verify that the EC50  found in the FR1 extract was 22.59 μg mL-1 and FR2 39.80 μg mL-1. Concluding that the EXTRACT FR2 showed strong antioxidant activity while FR1 was very strong by the DPPH radical method.

References

Bérdy, J. (2012). Thoughts and facts about antibiotics: where we are now and where we are heading. The Journal of antibiotics, 65(8), 385-395.

Bezerra, G. F. D. B., de Almeida, F. C., Neto da Silva, M. A. C., Nascimento, A. C. B., Meireles Guerra, R. N., Viana, G. M. D. C., ... & Brandão Nascimento, M. D. D. S. (2014a). Respiratory allergy to airborne fungi in São Luís–MA: clinical aspects and levels of IgE in a structured asthma program. Journal of Asthma, 51(10), 1028-1034.

Bezerra, G. F. D. B., Gomes, S. M., Silva, M. A. C. N. D., Santos, R. M. D., Muniz Filho, W. E., Viana, G. M. D. C., & Nascimento, M. D. D. S. B. (2014b). Diversity and dynamics of airborne fungi in São Luis, State of Maranhão, Brazil. Revista da Sociedade Brasileira de Medicina Tropical, 47(1), 69-73.

Bezerra, G. F.B., da Silva, M. A. C. N., dos Santos, R. M., Haidar, D. M. C., Muniz Filho, W. E., Rosa, I. G., ... & Nascimento, M. D. D. S. B. (2014). Avaliação da resposta IgE para o entendimento do papel de fungos do ar na alergia respiratória em crianças. Arquivos de Asma, Alergia e Imunologia, 2(3), 119-124.

Brae, S., Endnas, A., Keck, J., & Nising, C. F. (2009). Chemistry and biology of mycotoxins and related fungal metabo] ites. Chem Rev, 109, 3903-3990.

Brand-Williams, W., Cuvelier, M. E., & Berset, C. L. W. T. (1995). Use of a free radical method to evaluate antioxidant activity. LWT-Food science and Technology, 28(1), 25-30.

Brito, A., Ramirez, J. E., Areche, C., Sepúlveda, B., & Simirgiotis, M. J. (2014). HPLC-UV-MS profiles of phenolic compounds and antioxidant activity of fruits from three citrus species consumed in Northern Chile. Molecules, 19(11), 17400-17421.

Federation, W. E., & American Public Health Association. (2005). Standard methods for the examination of water and wastewater. American Public Health Association (APHA): Washington, DC, USA.

Mensor, L. L., Menezes, F. S., Leitão, G. G., Reis, A. S., Santos, T. C. D., Coube, C. S., & Leitão, S. G. (2001). Screening of Brazilian plant extracts for antioxidant activity by the use of DPPH free radical method. Phytotherapy research, 15(2), 127-130.

Nett, M., Ikeda, H., & Moore, B. S. (2009). Genomic basis for natural product biosynthetic diversity in the actinomycetes. Natural product reports, 26(11), 1362-1384.

Newman, D. J., & Cragg, G. M. (2016). Natural products as sources of new drugs from 1981 to 2014. Journal of natural products, 79(3), 629-661.

Qaisar, S., Bilal, R. M., Iqbal, W., Naureen, M., & Lee, S. (2013). Compressive sensing: From theory to applications, a survey. Journal of Communications and networks, 15(5), 443-456.

Rufino, M. D. S. M., Alves, R. E., de Brito, E. S., de Morais, S. M., Sampaio, C. D. G., Pérez-Jimenez, J., & Saura-Calixto, F. D. (2007). Metodologia científica: determinação da atividade antioxidante total em frutas pela captura do radical livre DPPH. Embrapa Agroindústria Tropical-Comunicado Técnico (INFOTECA-E).

Scherer, R., & Godoy, H. T. (2009). Antioxidant activity index (AAI) by the 2, 2-diphenyl-1-picrylhydrazyl method. Food chemistry, 112(3), 654-658.

Souza, T. J., Apel, M. A., Bordignon, S., Matzenbacher, N. I., Zuanazzi, J. Â. S., & Henriques, A. T. (2007). Composição química e atividade antioxidante do óleo volátil de Eupatorium polystachyum DC. Revista Brasileira de Farmacognosia, 17(3), 368-372.

Sucupira, N. R., da Silva, A. B., Pereira, G., & da Costa, J. N. (2012). Métodos para determinação da atividade antioxidante de frutos. Journal of Health Sciences, 14(4).

Tang, M. C., Cui, X., He, X., Ding, Z., Zhu, T., Tang, Y., & Li, D. (2017). Late-stage terpene cyclization by an integral membrane cyclase in the biosynthesis of isoprenoid epoxycyclohexenone natural products. Organic letters, 19(19), 5376-5379.

Teixeira, M. L., Cardoso, M. G., Souza, P. E., Machado, S. M. F., Andrade, M. A., Gomes, M. S., & Andrade, J. (2012). Citrumelo Swingle: Caracterização química, atividade antioxidante e antifúngica dos óleos essenciais das cascas frescas e secas. Magistra, 24, 194-203.

Vieira, D. A., Santos, P. S., Haminiuk, C. W. I., & Plata-Oviedo, M. S. V. (2011). Evaluation of the antioxidant activity of the leaves of acerola, guabiroba and ora-pro-nobis. Revista Brasileira de Pesquisa em Alimentos.

Waterhouse, W. C. (2012). Introduction to affine group schemes (Vol. 66). Springer Science & Business Media.

Wong, J. H., Sze, S. C., Ng, T. B., Cheung, R. C. F., Tam, C., Zhang, K. Y., ... & Waye, M. M. Y. (2018). Apoptosis and anti-cancer drug discovery: the power of medicinal fungi and plants. Current Medicinal Chemistry, 25(40), 5613-5630.

Published

17/07/2020

How to Cite

TELES, A. M.; EVERTON, G. O.; MOUCHREK, A. N.; TELLIS, C. J. M.; BEZERRA, G. F. de B.; SOUZA, F. A. de; SANTOS, A. P. S. de A.-.; NASCIMENTO, M. do D. S. B. Total phenolics and antioxidant activity of the extract obtained from the fungus Penicillium purpurogenum from polluted marine environment of Maranhão. Research, Society and Development, [S. l.], v. 9, n. 8, p. e576986205, 2020. DOI: 10.33448/rsd-v9i8.6205. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/6205. Acesso em: 26 apr. 2024.

Issue

Section

Health Sciences