Contamination by fungi and mycotoxins in wheat grains from Paraná and imported from Paraguay

Authors

DOI:

https://doi.org/10.33448/rsd-v9i9.8111

Keywords:

Zearalenone; Virus diseases.; Phytosanitary quality; Import.

Abstract

Wheat (Triticun aestivum L.) is one of the most produced cereals in the world. There are limitations such as excessive rainfall and high temperatures that facilitate the incidence of diseases, and in grain storage, an ideal microclimate is created, which can lead to the production of mycotoxins. Therefore, this work aimed to evaluate the phytosanitary quality of wheat grains in the West of Paraná and of imports from Paraguay. The wheat samples were collected from agricultural cooperatives, being 4 lots from Paraguay and 4 lots from Western Paraná. Mycotoxin analysis was performed through a feed analysis laboratory and fungal contamination analysis was performed at the PUCPR campus Toledo, by the filter paper method (Blotter test), by incubating the grains in gerbox boxes, with 50 seeds. After 7 days the fungus genera and the percentage of incidence of each were analyzed. The data were submitted to Kruskal - Wallis analysis to compare the means at the 5% significance level. Contaminant mycotoxins in the wheat lots of Western Paraná and Paraguay were not necessarily relevant for food safety according to the regulations and the Zearalenone index was higher for one of the lots from Paraguay. For the incidence of fungi a lot in western Paraná had higher contamination of Colletotrichum spp. and Fusarium spp., while for imports from Paraguay, it was for Rhizopus spp. and Bipolaris spp.

Author Biographies

Márcia de Holanda Nozaki, Pontifical Catholic University of Paraná

Doutora, Professora do curso de Agronomia

Arlindo Fabrício Corrêia, Pontifical Catholic University of Paraná

Doutor, Professor do curso de Agronomia

Larissa Aparecida Gonçalves, Pontifical Catholic University of Paraná

Graduando em Agronomia

Leandro Rampim, Midwestern State University

Doutor, professor no Departamento de Agronomia

References

Abitrigo. (2011). O triticultor e o mercado. Associação Brasileira Da Indústria Do Trigo. http://abitrigo.com.br/associados/arquivos/cartilha_triticutor.pdf

Abitrigo. (2019). Cartilha do agricultor - micotoxinas no trigo. Associação Brasileira Da Indústria Do Trigo. http://www.abitrigo.com.br/cloud/cartilha_do_agricultor_micotoxinas.pdf

Almeida-Ferreira, G. C., Barbosa-Tessmann, I. P., Sega, R., & Machinski Junior, M. (2013). Occurrence of zearalenone in wheat- and corn-based products commercialized in the state of paraná, Brazil. Brazilian Journal of Microbiology, 44(2), 371–375. https://doi.org/10.1590/S1517-83822013005000037

Regulamento técnico sobre limites máximos tolerados (LMT) para micotoxinas em alimentos, Pub. L. No. Resolução RDC no 7 (2011).

http://bvsms.saude.gov.br/bvs/saudelegis/anvisa/2011/res0007_18_02_2011_rep.html

Birck, N. M. M. (2005). Contaminação fúngica, micotoxinas e sua relação com a infestação de insetos em trigo (Triticum aestivum) pós colheita. Universidade Federal de Santa Catarina.

Bonfada, É. B., Honnef, D., Friedrich, M. T., Boller, W., & Deuner, C. C. (2019). Performance of fungicides on the control of fusarium head blight (Triticum aestivum L.) and deoxynivalenol contamination in wheat grains. Summa Phytopathology, 45(4), 374–380.

Brasil. (2009). Manual de Análise Sanitária de Sementes (Secretaria de Defesa Agropecuária (ed.)). Ministério da Agricultura, Pecuária e Abastecimento (MAPA).

Conab. (2017). A cultura do trigo. Companhia Nacional De Abastecimento. http://www.conab.gov.br

Costa, M. D. G. Da, Souza, E. L. De, Stamford, T. L. M., & Andrade, S. A. C. (2008). Qualidade tecnológica de grãos e farinhas de trigo nacionais e importados. Ciência e Tecnologia de Alimentos, 28(1), 220–225. http://www.scielo.br/pdf/cta/v28n1/30.pdf

Guterres, C. W., Seidel, G., Gubiani, D., Moreira, J., & Bastos, P. F. B. de. (2017). Levantamento de fungos associados às sementes de soja e trigo nas safras agrícolas 2015, 2016 e 2017 no Rio Grande do Sul. Mais Soja. https://maissoja.com.br/levantamento-de-fungos-associados-as-sementes-de-soja-e-trigo-nas-safras-agricolas-2015-2016-e-2017-no-rio-grande-do-sul/

Katsurayama, A. M., & Taniwaki, M. H. (2017). Fungos e aflatoxinas no arroz: ocorrência e significado na saúde do consumidor. Brazilian Journal of Food Technology, 20, 13. https://doi.org/10.1590/1981-6723.0617

Martins, M. L., & Martins, H. M. (2002). Influence of water activity, temperature and incubation time on the simultaneous production of deoxynivalenol and zearalenone in corn (Zea mays) by Fusarium graminearum. Food Chemistry, 79(3), 315–318. https://doi.org/10.1016/S0308-8146(02)00147-4

Mori, C. De, Antunes, J. M., Faé, G. S., & Acosta, A. da S. (2016). Trigo: O produtor pergunta, a Embrapa responde (1st ed.). Embrapa. https://ainfo.cnptia.embrapa.br/digital/bitstream/item/144769/1/ID43609-2016LVTrigo.pdf

Pereira, A. S., Shitsuka, D. M., Parreira, F. J., & Shitsuka, R. (2018). Metodologia da Pesquisa Científica (1st ed.). UFSM, NTE. https://repositorio.ufsm.br/bitstream/handle/1/15824/Lic_Computacao_Metodologia-Pesquisa-Cientifica.pdf?sequence=1. Acesso em: 28 março 2020.

Pires, J. L. F. (2014). Cultivo de Trigo. Embrapa Trigo. https://www.spo.cnptia.embrapa.br/conteudo?p_p_id=conteudoportlet_WAR_sistemasdeproducaolf6_1ga1ceportlet&p_p_lifecycle=0&p_p_state=normal&p_p_mode=view&p_p_col_id=column-2&p_p_col_count=1&p_r_p_-76293187_sistemaProducaoId=3704&p_r_p_-996514994_topicoId=3

Pires, J. L. F. (2017). A importância do trigo para a sustentabilidade da agricultura brasileira - Portal Embrapa. Empresa Brasileira de Pesquisa Agropecuária. https://www.embrapa.br/busca-de-noticias/-/noticia/23416523/artigo---a-importancia-do-trigo-para-a-sustentabilidade-da-agricultura-brasileira

Senes, C. E. R., Saldan, N. C., Costa, W. F., Svidzinskib, T. I. E., & Oliveira, C. C. (2018). Identification of Fusarium oxysporum Fungus in Wheat Based on Chemical Markers and Qualitative GC-MS Test. J. Braz. Chem. Soc., 29(12), 405–425.

Soares, V. N., Reis, B. B. dos, Almeida, A. da S., Nunes, A. de F., & Tunes, L. M. (2015). Análise sanitária de sementes de trigo durante o armazenamento. Centro Cientifíco Conhecer, Enciclopédia Biosfera, 11(22), 3562–3570.

Souza, T. M., Prando, A. M., Takabayashi-Yamashita, C. R., Zucareli, C., & Hirooka, E. Y. (2019). Chemical characterization of wheat kernels naturally contaminated by deoxynivalenol-DON when cultivated under nitrogen management strategies. Revista Ciência Agronômica, 50(4), 650–659. https://doi.org/10.5935/1806-6690.20190077.

Published

15/09/2020

How to Cite

Kolling, C. E., Nozaki, M. de H., Corrêia, A. F., Gonçalves, L. A., & Rampim, L. (2020). Contamination by fungi and mycotoxins in wheat grains from Paraná and imported from Paraguay. Research, Society and Development, 9(9), e982998111. https://doi.org/10.33448/rsd-v9i9.8111

Issue

Section

Agrarian and Biological Sciences