Immunohistochemical expression of macrophages in chronic periapical lesions

Authors

DOI:

https://doi.org/10.33448/rsd-v10i7.16622

Keywords:

Periapical granuloma; Radicular cyst; Immunohistochemistry; CD68 macrophage.

Abstract

Objective: To analyze the expression of macrophages in periapical granulomas (PGs) and radicular cysts (RCs). Methodology: We selected 264 cases of chronic periapical lesions stored at the Laboratory of Pathology, Dental School of Pernambuco (FOP)/UPE, including 89 PGs and 175 RCs. Seventy-nine cases, 23 PGs and 56 RCs, were submitted to immunohistochemical analysis by the streptavidin-biotin method using anti-CD68 antibody. The chi-square and Fisher’s exact tests were used to determine the existence of associations with categorical and clinical variables, adopting a 95% confidence level (p≤0.05). Results: We observed immunoexpression of CD68 in 83% of cases. The number of CD68+ cells (score 3 or 4) was higher in PGs, with predominantly intense staining. Significant differences in the number of CD68+ cells and staining intensity were observed between the chronic periapical lesions analyzed. Conclusion: The presence of CD68+ cells in periapical tissues may indicate the development, maintenance and/or severity of the inflammation-mediated immune response, which is more intense in PG.

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Published

24/06/2021

How to Cite

FARIAS, Z. B. B. M. de .; CAVALCANTE, J. S.; LIMA, A. C. de; ANDRADE, E. S. de S. .; SILVEIRA, M. M. F. da .; SOBRAL, A. P. V. . Immunohistochemical expression of macrophages in chronic periapical lesions. Research, Society and Development, [S. l.], v. 10, n. 7, p. e35310716622, 2021. DOI: 10.33448/rsd-v10i7.16622. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/16622. Acesso em: 22 nov. 2024.

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Section

Health Sciences