Biomonitorated study of Euphorbia umbellata (Pax) Bruyns extracts to be used to prepare silver nanoparticles with antimicrobial and cytotoxic potential
DOI:
https://doi.org/10.33448/rsd-v11i12.34804Keywords:
Terpenes; Silver Nanoparticles; Green Synthesis; Lung cancer; Antimicrobial Action; Antimicrobial action.Abstract
This study proposes to develop a biomonitorated study of the Euphorbia umbellata leaves extracts to identify those that could be used to prepare, by green synthesis, silver nanoparticles (AgNPs), with cytotoxic and antimicrobial potential. Hexane, dichloromethane, acetone, methanol, and aqueous extracts were evaluated to measure the terpenes amounts, antimicrobial activity (Klebsiella pneumoniae, Pseudomonas aeruginosa, Staphylococcus aureus), and the cytotoxic potential (A549, H292 cell lines). Characterization of AgNPs was performed by determination of zeta potential, polydispersity index, mean diameter, UV/Vis spectrometry, and scanning electron microscope (SEM), and evaluated against cytotoxic and antimicrobial models. Hexane and dichloromethane extracts presented the higher amounts of terpenes, exhibited microbiological inhibition against K. pneumoniae and S. aureus, and cytotoxic potential against both cell lines. AgNPs prepared with hexane and dichloromethane extracts were determined after 35 minutes of artificial light exposure and presented spherical form with aggregates, mean diameter of 177.9 ± 1.3 nm, 288.8 ± 1.4 nm, polydispersity index of 0.416, 0.496, and zeta potential -35.7, -30.5 mV, respectively. AgNPs obtained from dichloromethane extract incremented in the biological activities, with IC50 of 1.56 µg/mL and 0.25 µg/mL against K. pneumoniae and S. aureus, respectively, and IC50 of 0.28 µg/mL for A549 and 0.18 µg/mL for H292 cell lines. The development of AgNPs with dichloromethane extract of E. umbellata using green synthesis demonstrated high potential to be used as cytotoxic and antimicrobial product, associating the phytochemical composition and the presence of AgNPs in a synergic effect.
References
Abbasi, A., Emam-Djomeh, Z., Mousavi, M. A. E., & Davoodi, D. (2014). Stability of vitamin D3 encapsulated in nanoparticles of whey protein isolate. Food chemistry, 143, 379-383.
Ahmad, S., Perveen, S., Arshad, M. A., & Rehman, T. (2019). Pharmacological and nutritive potential of Euphorbia granulata. Journal of Complementary and Integrative Medicine, 16(2).
Alayande, S. O., Akinsiku, A. A., Akinsipo, O. B., Ogunjinmi, E. O., & Dare, E. O. (2021). Green synthesized silver nanoparticles and their therapeutic applications. Comprehensive Analytical Chemistry, 94, 585-611.
Andrade, E. A. D. (2020). Efeito citotóxico do látex de Euphorbia umbellata (Pax) Bruyns (Euphorbiaceae) para modelo de melanoma murino.
Azevedo, L. F. P., Faria, T. S. A., Pessanha, F. F., Araujo, M. F., & Lemos, G. C. S. (2014). Triagem fitoquímica e atividade antioxidante de Costus spicatus (Jacq.) Sw. Revista Brasileira de Plantas Medicinais, 16, 209-215.
Bamal, D., Singh, A., Chaudhary, G., Kumar, M., Singh, M., Rani, N., ... & Sehrawat, A. R. (2021). Silver nanoparticles biosynthesis, characterization, antimicrobial activities, applications, cytotoxicity and safety issues: an updated review. Nanomaterials, 11(8), 2086.
Banala, R. R., Nagati, V. B., & Karnati, P. R. (2015). Green synthesis and characterization of Carica papaya leaf extract coated silver nanoparticles through X-ray difextract, electron microscopy and evaluation of bactericidal properties. Saudi Journal of Biological Sciences, 22(5), 637-644.
Barabadi, H., Honary, S., Ali Mohammadi, M., Ahmadpour, E., Rahimi, M. T., Alizadeh, A., ... & Saravanan, M. (2017). Green chemical synthesis of gold nanoparticles by using Penicillium aculeatum and their scolicidal activity against hydatid cyst protoscolices of Echinococcus granulosus. Environmental Science and Pollution Research, 24(6), 5800-5810.
Cruz, L. S., Kanunfre, C. C., de Andrade, E. A., de Oliveira, A. A., Cruz, L. S., de Faria Moss, M., ... & Beltrame, F. L. (2020). Enriched terpenes extracts of the latex of euphorbia umbellata promote apoptosis in leukemic cells. Chemistry & Biodiversity, 17(9), e2000369.
Durgawale, P. P., Phatak, R. S., & Hendre, A. S. (2015). Biosynthesis of silver nanoparticles using latex of Syandenium grantii Hook f and its assessment of antibacterial activities. Digest Journal of Nanomaterials and Biostructures, 10(3), 847.
El-Mahmood, M. A. (2009). Antibacterial activity of crude extracts of Euphorbia hirta against some bacteria associated with enteric infections. Journal of Medicinal Plants Research, 3(7), 498-505.
Ernst, M., Nothias, L. F., Van der Hooft, J. J., Silva, R. R., Saslis-Lagoudakis, C. H., Grace, O. M., ... & Rønsted, N. (2019). Assessing specialized metabolite diversity in the cosmopolitan plant genus Euphorbia L. Frontiers in Plant Science, 10, 846.
Espadero, M., Avilés, H., Armijos, L., Ávila, L., Idrovo, L., Idrovo, M., & Oyola, C. (2019). Evaluación microbiológica y composición química de extractos orgánicos de Euphorbia aff. viridis (Klotzsch & Garcke) Boiss sobre Staphylococcus Aureus, Klebsiella Pneumoniae y Escherichia Coli. LA GRANJA. Revista de Ciencias de la Vida, 29(1), 119-129.
Espinoza, J. T. (2021). Desenvolvimento, caracterização e avaliação de lipossomas contendo β-sitosterol em um modelo experimental ex vivo de cataratogênese.
Guo, Y., Zhang, L., Zhang, S., Yang, Y., Chen, X., & Zhang, M. (2015). Fluorescent carbon nanoparticles for the fluorescent detection of metal ions. Biosensors and bioelectronics, 63, 61-71.
Jadranin, M., Pešić, M., Aljančić, I. S., Milosavljević, S. M., Todorović, N. M., Podolski-Renić, A., ... & Tešević, V. V. (2013). Jatrophane diterpenoids from the latex of Euphorbia dendroides and their anti-P-glycoprotein activity in human multi-drug resistant cancer cell lines. Phytochemistry, 86, 208-217.
Kanunfre, C. C., Leffers, T., Cruz, L. S., Luz, L. E., Crisma, A. R., Wang, M., ... & Beltrame, F. L. (2017). Euphorbia umbellata bark extracts–an in vitro cytotoxic study. Revista Brasileira de Farmacognosia, 27, 206-213.
Karaman, I., Şahin, F., Güllüce, M., Öǧütçü, H., Şengül, M., & Adıgüzel, A. (2003). Antimicrobial activity of aqueous and methanol extracts of Juniperus oxycedrus L. Journal of ethnopharmacology, 85(2-3), 231-235.
Kemboi, D., Peter, X., Langat, M., & Tembu, J. (2020). A review of the ethnomedicinal uses, biological activities, and triterpenoids of Euphorbia species. Molecules, 25(17), 4019.
Kumar, S. A., Priyadarshini, H. S., Paramita, A. P., Pinki, S., Sakshi, G., Kar, B. R., & Kiran, B. K. (2019). Green synthesis and biochemical characterization of silver nanoparticles by using Euphorbia umbellata leaf extract and analysis of antimicrobial activity against plant pathogens. GSC Biological and Pharmaceutical Sciences, 8(2).
Lee, S. H., & Jun, B. H. (2019). Silver nanoparticles: synthesis and application for nanomedicine. International journal of molecular sciences, 20(4), 865.
Lin, J., Dou, J., Xu, J., & Aisa, H. A. (2012). Chemical composition, antimicrobial and antitumor activities of the essential oils and crude extracts of Euphorbia
Luz, L. E. C., Kanunfre, C. C., Paludo, K. S., da Silva Justo, A., Petry, V. K., Lemes, B. M., ... & Beltrame, F. L. (2016). Cytotoxic biomonitored study of Euphorbia umbellata (Pax) Bruyns. Journal of ethnopharmacology, 183, 29-37.
Minozzo, B. R., Lemes, B. M., da Silva Justo, A., Lara, J. E., Petry, V. E. K., Fernandes, D., ... & Beltrame, F. L. (2016). Anti-ulcer mechanisms of polyphenols extract of Euphorbia umbellata (Pax) Bruyns (Euphorbiaceae). Journal of ethnopharmacology, 191, 29-40.
Momeni, S. S., Nasrollahzadeh, M., & Rustaiyan, A. (2016). Green synthesis of the Cu/ZnO nanoparticles mediated by Euphorbia prolifera leaf extract and investigation of their catalytic activity. Journal of colloid and interface science, 472, 173-179.
Munhoz, A. C., Minozzo, B. R., Cruz, L. S., Oliveira, T. L., Machado, W. M., Pereira, A. V., ... & Beltrame, F. L. (2014). Chemical and pharmacological investigation of the stem bark of Synadenium grantii. Planta Medica, 80(06), 458-464.
Nasrollahzadeh, M., Atarod, M., & Sajadi, S. M. (2016). Green synthesis of the Cu/Fe3O4 nanoparticles using Morinda morindoides leaf aqueous extract: a highly efficient magnetically separable catalyst for the reduction of organic dyes in aqueous medium at room temperature. Applied Surface Science, 364, 636-644.Nasrollahzadeh, M., Sajadi, S. M. (2016). Pd nanoparticles synthesized in situ with the use of Euphorbia granulate leaf extract: Catalytic properties of the resulting particles. Journal of Colloid and Interface Science, 462(15), 243-251.
Oliveira Junior, L. F. G., Santos, R. B., Reis, F. O., Matsumoto, S. T., Bispo, W. M. S., Machado, L. P., & Oliveira, L. F. M. (2013). Efeito fungitóxico do óleo essencial de aroeira da praia (Schinus terebinthifolius RADDI) sobre Colletotrichum gloeosporioides. Revista Brasileira de Plantas Medicinais, 15, 150-157.
Oliveira, T. L., Fontana, P. D., Bavia, L., Cruz, L. S., Crisma, A. R., Sassaki, G. L., ... & Messias-Reason, I. J. (2021). Effects of Euphorbia umbellata extracts on complement activation and chemotaxis of neutrophils. Journal of Ethnopharmacology, 265, 113348.
Papazisis, K. T., Geromichalos, G. D., Dimitriadis, K. A., & Kortsaris, A. H. (1997). Optimization of the sulforhodamine B colorimetric assay. Journal of immunological methods, 208(2), 151-158.
Patel, N. B., & Patel, K. C. (2014). Antibacterial activity of Euphorbia hirta L. ethanomedicinal plant against gram negative UTI pathogens. International Journal, 3(2), 24-29.
Salayová, A., Bedlovičová, Z., Daneu, N., Baláž, M., Lukáčová Bujňáková, Z., Balážová, Ľ., & Tkáčiková, Ľ. (2021). Green synthesis of silver nanoparticles with antibacterial activity using various medicinal plant extracts: Morphology and antibacterial efficacy. Nanomaterials, 11(4), 1005.
Vasas, A. & Hohmann, J. (2014). Euphorbia diterpenes: isolation, structure, biological activity, and synthesis (2008). Chemical Review, 114, 8579–8612
Yadi, M., Mostafavi, E., Saleh, B., Davaran, S., Aliyeva, I., Khalilov, R., ... & Milani, M. (2018). Current developments in green synthesis of metallic nanoparticles using plant extracts: a review. Artificial cells, nanomedicine, and biotechnology, 46(sup3), S336-S343.
Zhang, X. F., Liu, Z. G., Shen, W., & Gurunathan, S. (2016). Silver nanoparticles: synthesis, characterization, properties, applications, and therapeutic approaches. International journal of molecular sciences, 17(9), 1534.
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Copyright (c) 2022 Rosana Leticia Rosa; Patricia Mazureki Campos; Luiza Stolz Cruz; Robson Schimandeiro Novak ; Carla Cristine Kanunfre ; Luis Antônio Esmerino; Diego José Schebelski; Josiane de Fátima Padilha de Paula; Flavio Luís Beltrame
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