Camellia sinensis (green tea) promotes antifungal activity against Candida spp. isolated from HIV-positive patients and biocompatibility in murine macrophages
DOI:
https://doi.org/10.33448/rsd-v13i2.45002Keywords:
Amphotericin B; Biofilms; Camellia sinensis; Candida; Fluconazole.Abstract
The aim of this study was to evaluate the antifungal activity and cytotoxicity of the plant extract from Camellia sinensis (green tea) in vitro, as well as to assess the antifungal effect of amphotericin B and fluconazole on 22 strains of Candida spp. isolated from the oral cavity of HIV-positive patients. The minimum fungicidal concentration (MFC) and minimum inhibitory concentration (MIC) were determined in planktonic cells using serial dilutions of green tea extract and antifungals. After determining the extract concentration at MIC and MFC, biofilm was prepared for each strain. Cytotoxicity in mouse macrophages (RAW 264.7) was evaluated to assess the cellular viability of this substance. Colony-forming units (CFU/mL) were counted, and the data were statistically evaluated using the Mann-Whitney test (p<0.05) for biofilm, visual observation for MIC and MFC, and ANOVA and Tukey for cytotoxicity. The results demonstrated viability of green tea extract in the analyzed cells. It was concluded in this study that C. sinensis (green tea) extract showed antifungal activity in planktonic cells and in biofilm for all Candida strains evaluated, with no cytotoxic effects on RAW 264.7. Fluconazole exhibited fungicidal effect in planktonic cells, while amphotericin B showed antifungal effect on C. albicans strains and microbial resistance in non-albicans strains.
References
Antunes, D. P., Salvia, A. C., Araujo, R. M., Di Nicolo, R., Koga-Ito, C.Y. & Araujo M.A.M. (2010). Effect of green tea extract and mouthwash alcohol on Candida albicans biofilm on acrylic resin. Gerodontology, 32(4), 291-295. https://doi:10.11/ger.12132.
Appleton, S. S. (2000). Candidiasis: pathogenesis, clinical characteristics and treatment. Journal of the Californian Dental Association, 28(12):942-8. ID:11323949.
Aquino, V. R., Lunard, L. W., Goldani, L. Z. & Barth, A. L. (2005). Prevalence, susceptibility profile for fluconazole and risk factors for candidemia in a tertiary care hospital in southern Brazil. Brazilian Journal of Infectious Diseases, 9(5), 411-8. https://doi:10.1590/s1413.867020050005000009.
Blankenship, J.R. & Mitchell, A. P. (2006). How to build a biofilm: a fungal perspective. Current Opinion in Microbiology, 9(6), 588-94. https://doi:10.1016/j.mib.2006.10.003.
Casaroto, A. R. & Lara, V. S. (2009). Phytomedicines for Candida associated denture stomatitis. Fitoterapia, 81(5), 323-8. https://doi:10.1016/j.fitote.2009.12.003.
CLSI. Autoverification of Medical Laboratory Results for Specific Disciplines, 1st ed. CLSI guideline AUTO15. Clinical and Laboratory Standards Institute; 2019.
Coco, B. J., Cross, L. J., José, A., Cross, J. & Ramage, G. (2008). Mixed Candida albicans and Candida glabrata population associated with the pathogenesis of denture stomatitis. Oral Microbiology and Immunology, 23(5), 377-383. https://doi:10.1111/j.1399.302x.2008.00439.x.
Coleman, D. C., Gary, P. M., Mcmanus, B. A. & Sullivan, D. J. (2010). Mechanisms of antifungal drug resistence in Candida dubliniensis. Future Microbiology, 5(6), 935-949. https://doi:10.2217/fmb.10.51.
Eick, S., Glockmann, E., Brandl, B. & Pfister, E. W. (2004). Adherence of Streptococcus mutans to various restorative materials in a continuous flow systems. Journal of Oral Rehabilitation, 31(3), 278-5. https://doi:10.1046/j.0305.182x.2003.01233.x.
Enwuru, C. A., Ogunledun, A., Idika, N., Enwuru, N. V., Ogbonna, F., Aniedobe, M. & Adeiga, A. (2008). Fluconazole resistence opportunistic oro-pharyngeal Candida non-Candida yeast like isolates from HIV infected patients attending ARV clinics in Lagos, Nigéria. African Health Sciences, 8(3)142-8. ID: 2583271.
Gulati, M. & Nobile, C. J. (2016). Candida albicans biofilm: development, regulation and molecular mechanisms. Microbes Infection, 18(5), 310-321. https://doi:10.1016/J.MICINF.2016.01.002.
Hasan, F., Xess, I., Wang, X., Jain, N. & Fries, B. C. (2009). Biofilm formation in clinical Candida isolates and its association with virulence. Microbes Infection, 11(8),753-1. https://doi: 10.1016/j.micinf.2009.04.018.
Hospental, D. R., Beckieus, M. L., Floyd, K. L., Horvath, L.L. & Murray, C. K. (2005). Presentive identification of Candida species other Candida albicans, Candida krusei and Candida tropicalis with the chromogenic medium CHROM-ágar Candida. Annals of Clinical Microbiology and Antimicrobials, 5(1):1-5. https://doi:10.1186/1476.0711.5.1.
Jorge, A. O. C., Koga-Ito, C. Y., Gonçalves, C. R., Fantinato, V. & Unterkircher, C.S. (1997). Presença de leveduras do gênero Candida na saliva de pacientes com diferentes fatores predisponentes e de indivíduos controle. Revista de Odontologia da Universidade de São Paulo, 11(4), 279-285. https://doi.org/10.1590/S0103-06631997000400009.
Krcmery, V. & Barnes, A. J. (2002). Non-albicans Candida spp. causing fungaemia: pattogenicity and antifungal resistence. Journal of Hospital Infection, 50(4), 243-60. https://doi: 10.1053/jhin.2001.1151.
Perea, S., Ribot, J.L. L., Wickes, B. L., Kirkpatrick, W. R., Dib, O. P. & Bachmanm, S. P. (2002). Molecular mechanisms of fluconazole resistence in Candida dubliniensis isolates from HIV infected patients with oropharyngeal candidiasis. Anti Chemotherapeutic Agents, 46(6), 1695-703. https://doi: 10.1128/AAC.46.6.1695-1703.2002.
Pereira, A. V., Almeida, T. C., Beltrame, F. L., Costa, M. E. & Garrido, L. H. (2009). Determinação de compostos fenólicos em amostras comerciais de chá verde e preto (camellia sinensis). Acta Scientarium Health Sciences, 31(2), 119-4. https://doi.org/10.34119/bjhr2n5-081.
Panackal, A. A., Gribskov, J. L., Staab, J. F., Kirby, K. A., Rinaldi, M. & Marr, K.A. (2006). Clinical significance of azole antifungal drug cross-resistance in Candida glabrata. Journal Clinical Microbiology, 44(5), 1740-3. https://10.1128/JCM.44.5.1740-1743.2006.
Mollashahi, N. F., Bokaeiam, M., Mollashahi, L. F. & Afrougheh, A. (2015). Journal of Dentistry, Tehran University of Medical Sciences, 12(8.). PMC:4847165.
Matsuki, M., Kanatsu, H., Watanabe, T., Ogasawara, A., Mikami, T. & Matsumoto, T. (2006). Effects of antifungal drugs on proliferation signals in Candida albicans. Biological and Pharmaceutical Bulletin, 29(5), 919. https:// doi.org/ 10.1248/bpb.29.919.
Miller-Hamilton, J. M. T. (1995). Antimicrobial properties of tea (Camellia sinensis). Anti Chemotherapeutic Agents, 39(11), 2375-2377. https:// doi.org/10.11/AAC.39.11.2375.
Nadagir, S. D., Chunchanur, S. K., Halesh, L. H., Yasmeen, K., Chandrasekhar, M. R. & Patil, B. S. (2008). Significance of isolation and drug susceptibility testing of non-Candida albicans species causing oropharyngeal candidiasis in HIV patients. South Asian J Trop Med Public Health, 39(3), 492-5. ID: 18564689.
Nakata, H. M., Cadillo, E. M., Gastelu, J. V., Sanches, J. B. & Perfecto, D. R. (2007). Efeito antimicrobiano in vitro de la Camellia sinensis sobre bactéria orales. Odontología Sanmarquina, 10(1), 18-20. https://doi.org/10.15381/05.v10i1.2898.
Narotzki, B., Reznick, A. Z., Aizenud, D. & Yishai, L. (2012). Green tea: A promising natural product in oral health. Archives of Oral Biology, 57(5), 429-435. https://doi.org/10.1016/j.archoralbio.2011.11.07.
Oliveira, J. R., Castro, V. C., Vilela, P. G. F., Camargo, S. E.A., Carvalho, C. A. T., Jorge, A. O. C. & Oliveira, L. D. (2013). Cytotoxicity of Brazilian plant extracts against oral microorganisms of interest to dentistry. Complementary and Alternative Medicine, 13, 208. https//doi.org/10.1186/1472.6882.13.208.
Ramage, G., Coco, B., Rajedran, R., Rautema, R., Murray, C., Lappin, D. F. & Bagg, J. (2011). Commercial mouthwashes are more effective in vitro. Oral Surgery, oral medicine, oral pathology, oral radiology and endodontics, 8, 205-9. https//doi.org/10.1016/j.tripleo.2010.10.043.
Romeiro, R. L., Majewski, M., Molina, F., Junqueira, J. C., Oliveira, L. & Jorge, A. O. C. (2009). Aderência de C. albicans, C. dubliniensis e C. glabrata à superfície de implantes lisos e rugosos. Implantnews, 6(1), 33-37.
Samaranayake, Y. H., Samaranayake, L. P., Pow, E. H. N., Beena, V. T. & Yeung, K.W. S. (2001). Antifungal effects of lysozyme and lactoferrin against genetically similar, sequential Candida albicans isolates from a human immunodeficiency virus-infected southern Chinese cohort. Journal of Clinical Microbiology, 39(9), 3296-302. https//doi.org/10.1128/JCM.39.9.3296.3302.2001.
Serrano-Granger, C., Cerero-Lapiedra, R., Campo-Trapero, J. & Del RioHighsmith, J. (2005) In vitro study of the adherence of Candida albicans to acrylic resins: relationship to surface energy. International Journal of Prosthodontists, 18(5), 392-8.
Shivaprakasha, S., Radhakrishnan, K. & Karim, P. M. S. (2007). Candida ssp. other than Candida albicans: a major cause of fungaemia in a tertiary care center. Indian Journal of Medical Microbiology, 25(4), 405-7. https//doi.org/10.4103/0255.0857.37350.
Sitheeque, M. A. M., Panagoda, G. P., Yau, J., Amarakoun, A. M.T., Udagama, U.R. N. & Samaranayake, L. P. (2009). Antifungal activity of black tea polyphenois against Candida species. Chemotherapy, 55(3), 189-6. https//doi.org/10.1159?000216836.
Thein, Z. M., Samaranayake, Y. H. & Samaranayake, L. P. (2007). In vitro biofilm formation of Candida albicans and non-albicans Candida species under dynamic and anaerobic conditions. Archives of Oral Biology, 52(8), 761-7. https//doi.org/10.1016/j.archoralbio.2007.01.009.
Tsang, C. S. P. & Mcmillan, A. S. (2007). Antifungal susceptibility oh Candida albicans biofilms on titanium discs with different surface roughness. Clinical Oral Investigations, 11(4), 361-8. https//doi.org/10.1007/s00784.007.0122.3.
Vendruscolo, G. S., Rates, S. M. K. & Mentz, L. A. (2005). Dados farmacológicos sobre as plantas utilizadas como medicinais pela comunidade do bairro Ponta Grossa, Porto Alegre, Rio Grande do Sul. Brazilian Journal of Pharmacognosy,15(4), 361-2. https//doi.org/10.1590/S0012.695x2005000.40018.
Wang, Y. (2015). Looking into Candida albicans infection, host response, and antifungal strategies. Virulence, 6(4), 307-308. https//doi.org/10.1080/21505594.2014.1000752.
Wengeter, M. A., Guilhermetti, E., Shinobu, C. S., Takaki, I. & Svidzinski, T. I. E. (2007). Microbiological identification and in vitro sensitivity of Candida isolates from the oral cavity of HIV positive individuals. Revista da Sociedade Brasileira de Medicina Tropical, 40(3), 272-6. https//doi.org/10.1590/s0037.86822007000300004.
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