Comparison of chemical and physical decontamination of alginate dental impression material
DOI:
https://doi.org/10.33448/rsd-v13i10.46996Keywords:
Disinfection; Ultraviolet light; Dental impression materials; Biological contamination.Abstract
The decontamination of dental impression materials is essential to prevent cross-infections, and therefore different protocols and disinfection methods have been studied. The aim of this in vitro study was to evaluate the efficacy of ultraviolet light (UV-C) in microbial reduction of dental impression material (alginate – irreversible hydrocolloid). Circular alginate specimens were contaminated with a suspension of 10⁷ CFU/mL of Staphylococcus aureus and subjected to UV-C radiation (UV SURFACE, BioLambda, São Paulo, Brazil, λ = 254 nm) with different irradiation times, in addition to 1% sodium hypochlorite. The samples were divided into 4 groups: G1 - Control (n=3), G2 - 1% Sodium Hypochlorite for 10 min (n=3), G3 - UV-C radiation for 5 s (n=3), G4 - UV-C radiation for 10 s (n=3). Microbial samples were collected before and after decontamination methods, and the number of colony-forming units (CFU/mL) was determined. The data were statistically analyzed using ANOVA and Tukey tests (p<0.05). The results showed a significant reduction of S. aureus with both the chemical method and UV-C radiation. The 1% sodium hypochlorite achieved total eradication of microorganisms, while UV-C radiation effectively reduced the microbial load after 5 s of exposure. Increasing the exposure time did not result in a significant additional reduction. It is concluded that UV-C radiation is an effective and sustainable alternative, without generating chemical waste, assisting in the prevention of cross-infections in dental practice.
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