Comparison of acupuncture and low-level laser for myofascial pain: A pilot study randomized trial
DOI:
https://doi.org/10.33448/rsd-v14i4.48650Keywords:
Myofascial pain syndromes; Acupuncture; Laser therapy; Electromyography.Abstract
Myofascial pain in Temporomandibular Disorder (TMD) involves hypersensitive facial muscle trigger points, affecting quality of life. This trial aimed to evaluate the efficacy of acupuncture and low-level laser therapy for TMD-related pain relief. A randomized controlled trial with 14 participants (10 females, 4 males; mean age 29.5 years) divided into two groups (n=7). Group 1 received ten 20-minute acupuncture sessions every 48 hours, while Group 2 underwent low-power infrared laser therapy (790 nm, 120 mW) on the same schedule. Pain was assessed using a 0–10 Visual Analog Scale (VAS), and neuromuscular activity via bilateral electromyography (EMG) of the temporal and masseter muscles. Statistical analysis used SPSS 22.0 with Wilcoxon and paired t-tests (p<0.05). Both treatments significantly reduced subjective pain perception (VAS: Acupuncture 6.57 to 0.14; Laser 6.00 to 1.20), with no significant difference between groups. However, neither therapy significantly decreased neuromuscular activity in the temporal and masseter muscles, although acupuncture showed a trend toward reduced temporal muscle activity during clenching. Acupuncture and low-level laser therapy effectively reduced pain in patients with myofascial TMD. Despite differences in neuromuscular patterns, neither treatment significantly altered muscle activity, warranting further research to explore their underlying mechanisms.
References
Alghadir, A. H., Anwer, S., & Iqbal, Z. A. (2016). The psychometric properties of an Arabic numeric pain rating scale for measuring osteoarthritis knee pain. Disability and Rehabilitation, 38(24), 2392–2397.
Bashir, R. M., Abdulrahman, A., & Aliyu, S. (2020). Effectiveness of Bupivacaine Trigger Points Injections in the Treatment of Myofascial Pain Syndrome. Annals of Clinical and Experimental Medicine, 1(1), 23–28. https://doi.org/10.47838/acem.26011977.11162020.asmeda.4.0
Blanco-Aguilera, A., Blanco-Aguilera, E., Serrano-Del-Rosal, R., Biedma-Velázquez, L., Rodriguez-Torronteras, A., Segura-Saint-Gerons, R., & Blanco-Hungria, A. (2017). Influence of clinical and psychological variables upon the oral health-related quality of life in patients with temporomandibular disorders. Medicina Oral, Patologia Oral y Cirugia Bucal, 22(6), e669–e678. https://doi.org/10.4317/medoral.21746
Büyükşireci, D. E., Demirsoy, N., Mit, S., Geçioğlu, E., Onurlu, İ., & Günendi, Z. (2022). Comparison of the Effects of Myofascial Meridian Stretching Exercises and Acupuncture in Patients With Low Back Pain. Journal of Acupuncture and Meridian Studies, 15(6), 347–355. https://doi.org/10.51507/j.jams.2022.15.6.347
Cazal, M. S., da Silva, A. M. B. R., Galo, R., Junior, W. M., & da Silva, M. A. M. R. (2016). Comparison of dynamic electromyographic analysis of masticatory capsules with materials of different textures. Cranio : The Journal of Craniomandibular Practice, 34(2), 105–111. https://doi.org/10.1179/2151090315Y.0000000003
Conti, P. C. R., Costa, Y. M., Gonçalves, D. A., & Svensson, P. (2016). Headaches and myofascial temporomandibular disorders: overlapping entities, separate managements? Journal of Oral Rehabilitation, 43(9), 702–715. https://doi.org/10.1111/joor.12410
Coyle, S. (2022). Pridinol for Cancer-Related Myofascial Pain. BMJ Supportive & Palliative Care, 13(e3), e945–e947. https://doi.org/10.1136/spcare-2022-003821
Cuschieri, S. (2019). The CONSORT statement. Saudi Journal of Anaesthesia, 13(Suppl 1), S27–S30. https://doi.org/10.4103/sja.SJA_559_18
Dietrich, L., Rodrigues, I. V. S., Costa, M. D. M. de A., Carvalho, R. F., & Silva, G. R. d. (2020). Acupuncture in Temporomandibular Disorders Painful Symptomatology: An Evidence-Based Case Report. European Journal of Dentistry, 14(04), 692–696. https://doi.org/10.1055/s-0040-1716631
Dommerholt, J., Chou, L.-W., Finnegan, M., & Hooks, T. (2018). A critical overview of the current myofascial pain literature - April 2018. In Journal of bodywork and movement therapies (Vol. 22, Issue 2, pp. 402–410). https://doi.org/10.1016/j.jbmt.2018.04.001
Fikácková, H., Dostálová, T., Navrátil, L., & Klaschka, J. (2007). Effectiveness of low-level laser therapy in temporomandibular joint disorders: a placebo-controlled study. Photomedicine and Laser Surgery, 25(4), 297–303. https://doi.org/10.1089/pho.2007.2053
Fricton, J. (2016). Myofascial Pain: Mechanisms to Management. Oral and Maxillofacial Surgery Clinics of North America, 28(3), 289–311. https://doi.org/10.1016/j.coms.2016.03.010
Georgoudis, G., Felah, B., Nikolaidis, P. T., Παπανδρέου, Μ., Mitsiokappa, E., Mavrogenis, A. F., Rosemann, T., & Knechtle, B. (2018). The Effect of Physiotherapy and Acupuncture on Psychocognitive, Somatic, Quality of Life, and Disability Characteristics in TTH Patients. Journal of Pain Research, Volume 11, 2527–2535. https://doi.org/10.2147/jpr.s178110
Gerwin, R. D. (2014). Diagnosis of myofascial pain syndrome. Physical Medicine and Rehabilitation Clinics of North America, 25(2), 341–355. https://doi.org/10.1016/j.pmr.2014.01.011
Hugger, S., Schindler, H. J., Kordass, B., & Hugger, A. (2013). Surface EMG of the masticatory muscles. (Part 4): Effects of occlusal splints and other treatment modalities. International Journal of Computerized Dentistry, 16(3), 225–239.
Kothari, C., Clemenceau, A., Ouellette, G., Ennour-Idrissi, K., Michaud, A., C.-Gaudreault, R., Diorio, C., & Durocher, F. (2021). TBC1D9: an important modulator of tumorigenesis in breast cancer. Cancers, 13(14), 3557.
Lee, S.-Y., Lee, I.-S., & Chae, Y. (2022). Similarities Between Ashi Acupoints and Myofascial Trigger Points: Exploring the Relationship Between Body Surface Treatment Points. Frontiers in Neuroscience, 16. https://doi.org/10.3389/fnins.2022.947884
List, T., & Helkimo, M. (1987). Acupuncture in the treatment of patients with chronic facial pain and mandibular dysfunction. Swedish Dental Journal, 11(3), 83–92.
Loeser, J. D., & Ballantyne, J. C. (2024). Pains Revisited. The Clinical Journal of Pain, 40(8), 459–462. https://doi.org/10.1097/AJP.0000000000001224
MacPherson, H., Altman, D. G., Hammerschlag, R., Youping, L., Taixiang, W., White, A., & Moher, D. (2010). Revised STandards for Reporting Interventions in Clinical Trials of Acupuncture (STRICTA): extending the CONSORT statement. PLoS Medicine, 7(6), e1000261. https://doi.org/10.1371/journal.pmed.1000261
Manolopoulos, L., Vlastarakos, P. V, Georgiou, L., Giotakis, I., Loizos, A., & Nikolopoulos, T. P. (2008). Myofascial pain syndromes in the maxillofacial area: a common but underdiagnosed cause of head and neck pain. International Journal of Oral and Maxillofacial Surgery, 37(11), 975–984. https://doi.org/10.1016/j.ijom.2008.04.023
Melzack, R. (1981). Myofascial trigger points: relation to acupuncture and mechanisms of pain. Archives of Physical Medicine and Rehabilitation, 62(3), 114–117.
Mohammed, E., Baiomy, A. A. B. A., Farhat, M., & Helal, M. A. (2022). Effect of Four Conservative Treatment Methods on the Muscular Activity of Masseter and Temporalis Muscles for Subjects With Temporomandibular Joint Disc Displacement With Reduction. Al-Azhar Journal of Dental Science, 25(4), 401–408. https://doi.org/10.21608/ajdsm.2021.58653.1149
Nielsen, A., Dusek, J. A., Taylor-Swanson, L., & Tick, H. (2022). Acupuncture Therapy as an Evidence-Based Nonpharmacologic Strategy for Comprehensive Acute Pain Care: The Academic Consortium Pain Task Force White Paper Update. Pain Medicine, 23(9), 1582–1612. https://doi.org/10.1093/pm/pnac056
Palinkas, M., Bataglion, C., de Luca Canto, G., Machado Camolezi, N., Theodoro, G. T., Siéssere, S., Semprini, M., & Regalo, S. C. H. (2016). Impact of sleep bruxism on masseter and temporalis muscles and bite force. Cranio : The Journal of Craniomandibular Practice, 34(5), 309–315. https://doi.org/10.1080/08869634.2015.1106811
Pereira A. S. et al. (2018). Metodologia da pesquisa científica. [free e-book]. Santa Maria/RS. Ed. UAB/NTE/UFSM.
Pereira, T. S., Flecha, O. D., Guimarães, R. C., de Oliveira, D., Botelho, A. M., Ramos Glória, J. C., & Aguiar Tavano, K. T. (2014). Efficacy of red and infrared lasers in treatment of temporomandibular disorders--a double-blind, randomized, parallel clinical trial. Cranio : The Journal of Craniomandibular Practice, 32(1), 51–56. https://doi.org/10.1179/0886963413Z.0000000005
Rancan, S. V., Bataglion, C., Bataglion, S. A., Bechara, O. M. R., Semprini, M., Siéssere, S., Sousa, J. P. M. de, Crippa, J. A. de S., Hallak, J. E. C., & Regalo, S. C. H. (2009). Acupuncture and temporomandibular disorders: a 3-month follow-up EMG study. Journal of Alternative and Complementary Medicine (New York, N.Y.), 15(12), 1307–1310. https://doi.org/10.1089/acm.2009.0015
Randhawa, K., Bohay, R., Côté, P., van der Velde, G., Sutton, D., Wong, J. J., Yu, H., Southerst, D., Varatharajan, S., Mior, S., Stupar, M., Shearer, H. M., Jacobs, C., & Taylor-Vaisey, A. (2016). The Effectiveness of Noninvasive Interventions for Temporomandibular Disorders: A Systematic Review by the Ontario Protocol for Traffic Injury Management (OPTIMa) Collaboration. The Clinical Journal of Pain, 32(3), 260–278. https://doi.org/10.1097/AJP.0000000000000247
Salles‐Neto, F. T. d., Paula, J. S. d., Romero, J. G. de A. J., & Almeida‐Leite, C. M. (2020). Acupuncture for Pain, Mandibular Function and Oral Health‐related Quality of Life in Patients With Masticatory Myofascial Pain: A Randomised Controlled Trial. Journal of Oral Rehabilitation, 47(10), 1193–1201. https://doi.org/10.1111/joor.13055
Salmos-Brito, J. A. L., de Menezes, R. F., Teixeira, C. E. C., Gonzaga, R. K. M., Rodrigues, B. H. M., Braz, R., Bessa-Nogueira, R. V., & Gerbi, M. E. M. de M. (2013). Evaluation of low-level laser therapy in patients with acute and chronic temporomandibular disorders. Lasers in Medical Science, 28(1), 57–64. https://doi.org/10.1007/s10103-012-1065-8
Sanzarello, I., Merlini, L., Rosa, M. A., Perrone, M., Frugiuele, J., Borghi, R., & Faldini, C. (2016). Central sensitization in chronic low back pain: a narrative review. Journal of Back and Musculoskeletal Rehabilitation, 29(4), 625–633.
Saraspuri, N. P. E., Tamtomo, D. G., & Murti, B. (2022). Effectiveness of Acupuncture Therapy to Reduce Pain in Patients With Upper Trapezius Myofascial Pain Syndrome: A Meta-Analysis. Indonesian Journal of Medicine, 7(3), 326–336. https://doi.org/10.26911/theijmed.2022.07.03.09
Sattayut, S., & Bradley, P. (2012). A study of the influence of low-intensity laser therapy on painful temporomandibular disorder patients. Laser Therapy, 21(3), 183–192. https://doi.org/10.5978/islsm.12-OR-09
Saxena, A., Chansoria, M., Tomar, G., & Kumar, A. (2015). Myofascial pain syndrome: an overview. Journal of Pain & Palliative Care Pharmacotherapy, 29(1), 16–21. https://doi.org/10.3109/15360288.2014.997853
Shitsuka, R. et al. (2014). Matemática fundamental para tecnologia. 2ed. Editora Erica.
Shukla, D., & Muthusekhar, M. R. (2016). Efficacy of low-level laser therapy in temporomandibular disorders: A systematic review. National Journal of Maxillofacial Surgery, 7(1), 62–66. https://doi.org/10.4103/0975-5950.196127
Tardelli, J. D. C., Gubitoso, B., Botelho, A. L., Valente, M. L. da C., & Reis, A. C. Dos. (2024). Efficacy of acupuncture on craniomandibular myofascial pain in temporomandibular disorder patients: A systematic review. Heliyon, 10(13), e32075. https://doi.org/10.1016/j.heliyon.2024.e32075
Toassi, R. F. C. & Petry, P. C. (2021). Metodologia científica aplicada à área da Saúde. 2ed. Porto Alegre: Editora da UFRGS.
Venezian, G. C., da Silva, M. A. M. R., Mazzetto, R. G., & Mazzetto, M. O. (2010). Low level laser effects on pain to palpation and electromyographic activity in TMD patients: a double-blind, randomized, placebo-controlled study. Cranio : The Journal of Craniomandibular Practice, 28(2), 84–91. https://doi.org/10.1179/crn.2010.012
Vieira, S. (2021). Introdução à bioestatística. Ed.GEN/Guanabara Koogan.
Vilanova, L. S., Gonçalves, T. M., Meirelles, L., & Garcia, R. C. (2015). Hormonal fluctuations intensify temporomandibular disorder pain without impairing masticatory function. The International Journal of Prosthodontics, 28(1), 72–74. https://doi.org/10.11607/ijp.4040
Wager, T. D., Atlas, L. Y., Lindquist, M. A., Roy, M., Woo, C.-W., & Kross, E. (2013). An fMRI-based neurologic signature of physical pain. The New England Journal of Medicine, 368(15), 1388–1397. https://doi.org/10.1056/NEJMoa1204471
Wang, C. C., Huang, T.-H., Chiou, K. C., & Chang, Z. Y. (2018). Therapeutic Effect of Superficial Acupuncture in Treating Myofascial Pain of the Upper Trapezius Muscle: A Randomized Controlled Trial. Evidence-Based Complementary and Alternative Medicine, 2018(1). https://doi.org/10.1155/2018/9125746
Wieckiewicz, M., Zietek, M., Smardz, J., Zenczak-Wieckiewicz, D., & Grychowska, N. (2017). Mental Status as a Common Factor for Masticatory Muscle Pain: A Systematic Review. Frontiers in Psychology, 8, 646. https://doi.org/10.3389/fpsyg.2017.00646
Witt, C. M., Pach, D., Reinhold, T., Wruck, K., Brinkhaus, B., Mank, S., & Willich, S. N. (2011). Treatment of the adverse effects from acupuncture and their economic impact: a prospective study in 73,406 patients with low back or neck pain. European Journal of Pain (London, England), 15(2), 193–197. https://doi.org/10.1016/j.ejpain.2010.06.008
Yassaei, S., Aghili, H., Salarpour, E., & Dehghan Dehnavi, A. (2022). Electromyographic Evaluation of Superior Orbicularis Oris Muscle Activity in Patients With Complete Cleft Lip and Cleft Palate Before and After Vestibuloplasty Using Carbon Dioxide Laser Therapy. Journal of Lasers in Medical Sciences, 13, e67. https://doi.org/10.34172/jlms.2022.67
Zhang, X., Chen, H., Xu, W., Song, Y., Gu, Y., & Ni, G.-X. (2019). ≪p>Acupuncture Therapy for Fibromyalgia: A Systematic Review and Meta-Analysis of Randomized Controlled Trials</P> Journal of Pain Research, Volume 12, 527–542. https://doi.org/10.2147/jpr.s186227
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2025 Renan Leonardi de Oliveira Rigotti; Luana de Góes Soares; Larissa Doalla de Almeida-Silva; Ana Carolina Coelho-Oliveira; Simone Gomes Dias-Oliveira; Fábio Luiz Mendonça Martins; Rodrigo Galo

This work is licensed under a Creative Commons Attribution 4.0 International License.
Authors who publish with this journal agree to the following terms:
1) Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under a Creative Commons Attribution License that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
2) Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
3) Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work.