Are there differences in the frequency of micronuclei in Astyanax lacustris in relation to sex, mass and length?
DOI:
https://doi.org/10.33448/rsd-v9i8.5151Keywords:
Aquatic environment; Contamination; Genotoxicity; Fish; Biometry.Abstract
The aim of this study was to analyze genotoxic effects in Astyanax lacustris and to test whether there are differences in the frequency of micronuclei in relation to sex, mass and length of individuals. The samples were carried out in the Tarumã Microbasin (Upper Paraná River, State of Mato Grosso do Sul, Brazil). In this study, we described this species presented the same relative frequency of micronuclei for both sexes, and for individuals of different masses and lengths.
References
Abdalla, R. P., Kida, B. M. S., Pinheiro, J. P. S., Oliveira, L. F., Martinez, C. B. F., & Moreira, R. G.. (2019). Exposure to aluminum, aluminum + manganese and acid pH triggers different antioxidant responses in gills and liver of Astyanax altiparanae (Teleostei: Characiformes: Characidae) males. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 215:33-40. Https://doi.org/10.1016/j.cbpc.2018.09.004.
Chehade, C. C., Borella, M., Costa, M. I., & Gonçalves, F. (2014). Morphologic study of the liver of lambari (Astyanax altiparanae) with emphasis on the distribution of cytokeratin. Fish Physiology and Biochemistry, 40:571-6. https://doi.org/10.1007/s10695-013-9867-6.
Corduk, N., Hacioglu-Dogru, N., Gul, C., & Tosunoglu, M. (2018). Monitoring of micronuclei and nuclear abnormalities in Pelophylax ridibundus erythrocytes from the biga stream (Canakkale, Turkey). Fresen Environ Bull, 27:147-153.
Dar, S. A., Yousuf, A. R., & Balkhi, M. H. (2016). An introduction about genotoxicology methods as tools for monitoring aquatic ecosystem: present status and future perspectives. Fish Aquac J, 7:1-11. https://doi.org/10.4172/2150-3508.1000158.
Darzynkiewicz, Z., Huang, X., & Zhao, H.. (2017). Analysis of cellular DNA content by flow cytometry. Curr Protoc Immunol, 119:5-7. https://doi.org/10.1002/cpcy.28.
Dourado, P. L., Rocha, M. P., Roveda, L. M., Raposo, J. L., Junior- Cândido, L. S., Cardoso, C. A., Morales, M. A., Oliveira, K. M., & Grisolia, A. B. (2016) Genotoxic and mutagenic effects of polluted surface water in the midwestern region of Brazil using animal and plant bioassays. Genet Mol Biol, 40:123-133. https://doi. org/10.1590/1678-4685-GMB-2015-0223.
Fenech, M., Chanq, W. P., Kirsch-Volders, M., Holland, N., Bonassi, S., & Zeiger, E. (2003). HUMN project: detailed description of the scoring criteria for the cytokinesis-block micronucleus assay using isolated human lymphocyte cultures. Mutat Rese, 534:65-75. https ://doi.org/10.1016/S1383-5718(02)00249-8.
Hariri, M., Mirvaghefi, A., Farahmand, H., Taghavi, L., & Shahabinia, A. R. (2018). In situ assessment of Karaj River genotoxic impact with the alkaline comet assay and micronucleus test, on feral brown trout (Salmo trutta fario). Environmental Toxicology and Pharmacology, 58:59-69. https://doi.org/10.1016/j.etap.2017.12.024.
Hussain, B., Sultana, T., Sultana, S., Al-Ghanim, K. A., Al-Misned, F., & Mahboob, S. (2019). Influence of habitat degradation on the fatty acid profiles of fish, Microalgae, and Zoobenthos in a River Ecosystem. Process Safety and Environmental Protection, 123: 24-32. https://doi.org/10.1016/j.psep.2018.12.024.
Jesus, I. S., Cestari, M. M., Bezerra, M. A., & Afonso, P. R. A. M. (2016). Genotoxicity effects in freshwater fish from a Brazilian impacted river. Bull Environ Contam Toxicol, 96:490-495. https://doi. org/10.1007/s00128-016-1755-1.
Lima, L. B. D., Morais, P. B., Andrade, R. L. T., Mattos, L. V., & Moron, S. E. (2018). Use of biomarkers to evaluate the ecological risk of xenobiotics associated with agriculture. Environmental Pollution, 237:611-624. https://doi.org/10.1016/j.envpol.2018.02.011.
Lima, E. L. R., Severi, W., & Lopes, J. P. (2011). Qualidade da água e dos efluentes em viveiros de alevinagem de Astyanax lacustris, Reinhardt, 1874. Ciência Animal, 21:07-16.
Lucena, C. A. S., & Soares, H. G. (2016). Review of species of the Astyanax bimaculatus caudal peduncle spot subgroup sensu Garutti & Langeani (Characiformes, Characidae) from the rio La Plata and rio São Francisco drainages and coastal systems of southern Brazil and Uruguay. Zootaxa, 4072:101-125. https://doi.org/ 10.11646/zootaxa.4072.1.5.
Milanin, T., Mathews, P. D., Mertins, O., Tavares, L. E. R., Silva, M. R. M., & Maia, A. A. M. (2018). Molecular phylogeny of the gill parasite Henneguya (Myxosporea: Myxobolidae) infecting Astyanax lacustris (Teleostei: Characidae) from fish farm in Brazil. Microb Pathog, 123:372-376. https://doi.org/10.1016/j.micpath.2018.07.037.
Monteiro, J. A. D. N., Cunha, L. A. D., Costa, M. H. P. D., Reis, H. S. D, Aguiar, ACDS, Oliveira-Bahia, VRL, Burbano, RMR & Rocha, CAMD. (2018). Mutagenic and histopathological effects of hexavalent chromium in tadpoles of Lithobates catesbeianus (Shaw, 1802) (Anura, Ranidae). Ecotoxicology and Environmental Safety, 163:400-407. https://doi.org/10.1016/j.ecoenv.2018.07.083.
Pandey, A. K., Nagpure, N. S., & TRivedi, S. P. (2018). Genotoxicity assessment of pesticide profenofos in freshwater fish Channa punctatus (Bloch) using comet assay and Random Amplified Polymorphic DNA (RAPD). Chemosphere, 211:316-323. https://doi.org/ 10.1016/j.chemosphere.2018.07.182.
Pereira, A. S., Shitsuka, D. M., Parreira, F. J., & Shitsuka, R. (2018). Metodologia da pesquisa científica. [e-book]. Santa Maria. Ed. UAB/NTE/UFSM. Disponível em: https://repositorio.ufsm.br/bitstream/handle/1/15824/Lic_Computacao_Metodologia-Pesquisa-Cientifica.pdf?sequence=1.
Pinheiro, J. P. S., Assis, C. B., Muñoz-Peñuela, M., Barbosa, J. R. F., Correia, T. G., & Moreira, R. G. (2019). Water temperature and acid ph influence the cytotoxic and genotoxic effects of aluminum in the freshwater teleost astyanax altiparanae (teleostei: characidae). chemosphere, 220:266-274. https://doi.org/10.1016/j.chemosphere.2018.12.143.
Procópio, M. S., Ribeiro, H. J., Pereira, L. A., Oliveira, L., G. A., Castro, A. C., Rizzo, E., Sato, Y., Russo, R. C., & Corrêa, J. D. Jr. (2014). Sex-response differences of immunological and histopathological biomarkers in gill of prochilodus argenteus from a polluted river in southeast brazil. fish shellfish immunol, 39:108-17. https://doi.org/ 10.1016/j.fsi.2014.04.010.
R Core Team. (2019). R: a Language and environment for statistical computing. R Foundation For Statistical Computing, Vienna Http://www. R-project.org.
Schmid, W. (1975). The micronucleus test. Mutat Res, 31:9-15.
Siqueira-Silva, D. H., Silva, A. P. S., Ninhaus-Silveira, A., & Veríssimo Silveira, R. (2015). Morphology of the urogenital papilla and its components ducts in Astyanax altiparanae Garutti and Britski, 2000 (Characiformes: Characidae). Neotrop Ichthyol, 13:309-316. doi: http://dx.doi.org/10.1590/1982-0224-20140102.
Sposito, J. C. V., Francisco, L. F. V., Do Amaral Crispim, B., Da Silva Dantas, F. G., De Souza, J. P., Viana, L. F., Solórzano, J. C. J., De Oliveira, K. M. P., & Barufatti, A.. (2019).
Influence of Land Use and Cover on Toxicogenetic Potential of Surface Water from Central-West Brazilian Rivers. Archives of Environmental Contamination and Toxicology, 76:483-495. https://doi.org/10.1007/s00244-019-00603-2.
Trujillo-Jiménez, P., Sedeño-Díaz, J. E., Camargo, J. Á., & López-López, E. (2011). Assessing environmental conditions of the Río Champotón (México) using diverse indices and biomarkers in the fish Astyanax aeneus (Günther, 1860). Ecol Indic, 11:1636–1646. https:// doi.org/10.1016/j.ecolind.2011.04.007.
Viana, L. F., Súarez, Y. R., Cardoso, C. A. L., Solórzano, J. C. J., Crispim, B. D. A., Grisolia, A. B., & Lima-Junior, S. E. (2017). Erythrocyte nuclear abnormalities in Astyanax lacustris in response to landscape characteristics in two neotropical streams. Arch Environ Contam Toxicol, 17:1-8. https://doi.org/10.1007/s00244-017-0476-8.
Viana, L. F., Súarez, Y. R., Cardoso, C. A. L., Crispim, B. D. A., Cavalcante, D. N. C., Grisolia, A. B., & Lima-Junior, S. E. (2018). The Response of Neotropical Fish Species (Brazil) on the Water Pollution: Metal Bioaccumulation and Genotoxicity. Arch Environ Contam Toxicol, 75:476-485. https://doi.org/10.1007/s00244-018-0551-9.
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