In vitro trypanocidal and cytotoxic activity of essential oils from native plants of the brazilian cerrado
DOI:
https://doi.org/10.33448/rsd-v9i9.7072Keywords:
Chagas disease; Trypanosoma cruzi; Brazilian Cerrado plants; Essential oil; Antiparasitic drugs.Abstract
Chagas disease (CD) is a major neglected tropical disease that is caused by Trypanosoma cruzi (Chagas, 1909). CD is treated with the antiparasitic drugs nifurtimox and benznidazole, which have several side effects. The market for natural products has grown and the use of plants has emerged as an alternative for the development of novel drugs to cure this disease. In this context, this study reports the chemical analysis of the essential oils from the leaves of Zanthoxylum riedelianum (Rutaceae), Zanthoxylum rhoifolium (Rutaceae) and Kielmeyera coriacea (Calophyllaceae) and the fruits of Xilopia aromática (Annonaceae) and Zanthoxylum rhoifolium (Rutaceae), as well as the trypanocidal and cytotoxic activities of these. The chemical analysis of the oils was performed by gas chromatography coupled to mass spectrometry. The trypanocidal assay was performed on trypomastigote forms of Trypanosoma cruzi, and the evaluation of the cytotoxic activity was performed on the LLCMK2 cell line. The essential oils with stronger trypanocidal activity were those from the leaves of K. coriacea (IC50 = 6.4 µg.mL-1) and fruits of X. aromatica (IC50 = 6.4 µg.mL-1), followed by those from the fruits of Z. rhoifolium (IC50 = 8.1 µg.mL-1). The latter two essentials oils showed a better selectivity index (SI). Additionally, sabinene may be responsible for these properties since it is the major compound present in this essentials oils. The cytotoxic analysis indicated that all of the essential oils evaluated were toxic to the LLCMK2 cells at concentrations higher than 100 µg.mL-1 and therefore they are excellent candidates for the development of novel antiparasitic drugs.
References
Adams, R. P. (2007). Identification of Essential Oil Components By Gas Chromatography / Mass Spectrometry (4th ed.). Allured Pub Corp.
Borges, A. R., Aires, J. R. de A., Higino, T. M. M., Medeiros, M. das G. F. de, Citó, A. M. das G. L., Lopes, J. A. D., & Figueiredo, R. C. B. Q. de. (2012). Trypanocidal and cytotoxic activities of essential oils from medicinal plants of Northeast of Brazil. Experimental Parasitology, 132(2), 123–128.
Brasil, Ministério da Saúde, & Secretaria de Vigilância em Saúde. (2019). Doença de Chagas Aguda e distribuição espacial dos triatomíneos de importância epidemiológica, Brasil 2012 a 2016. Boletim Epidemiológico 02, 50(2), 1–10.
Carneiro, N. S., Alves, J. M., Alves, C. C. F., Esperandim, V. R., & Miranda, M. L. D. (2017). Artigo Óleo Essencial das Flores de Eugenia klotzschiana ( Myrtaceae ): Composição Química e Atividades Tripanocida e Citotóxica In Vitro Óleo Essencial das Flores de Eugenia klotzschiana ( Myrtaceae ): Composição Química e Atividades Tripanocida e Citotó. Revista Virtual de Quimica, 9(3).
Cimerman, B., & Cimerman, S. (2010). Parasitologia humana e seus fundamentos gerais (2nd ed.). Atheneu Editor.
Da Silva, T. B., Menezes, L. R. A., Sampaio, M. F. C., Meira, C. S., Guimarães, E. T., Soares, M. B. P., Do Prata, A. P. N., De Nogueira, P. C. L., & Costa, E. V. (2013). Chemical composition and anti-trypanosoma cruzi activity of essential oils obtained from leaves of Xylopia frutescens and X. laevigata (Annonaceae). Natural Product Communications, 8(3), 403–406.
De Melo, D. C., Miranda, M. L. D., Ferreira Júnior, W. G., de Andrade, P. M., Alcoba, A. E. T., Silva, T. de S., Cazal, C. de M., & Martins, C. H. G. (2017). Anticariogenic and antimycobacterial activities of the essential oil of siparuna guianensis aublet (Siparunaceae). Orbital, 9(1), 55–60.
Dias, J. C. P., Ramos, A. N., Gontijo, E. D., Luquetti, A., Shikanai-Yasuda, M. A., Coura, J. R., Torres, R. M., Melo, J. R. da C., Almeida, E. A. de, Oliveira, W. de, Silveira, A. C., Rezende, J. M. de, Pinto, F. S., Ferreira, A. W., Rassi, A., Fragata, A. A., Sousa, A. S. de, Correia, D., Jansen, A. M., … Alves, R. V. (2016). Aspectos Gerais da Epidemiologia da Doença de Chagas com Especial Atenção ao Brasil. Epidemiologia e Servicos de Saude : Revista Do Sistema Unico de Saude Do Brasil, 25, 7–86.
Esperandim, V. R., Da Silva Ferreira, D., Sousa Rezende, K. C., Magalhães, L. G., Medeiros Souza, J., Pauletti, P. M., Januário, A. H., Da Silva De Laurentz, R., Bastos, J. K., Símaro, G. V., Cunha, W. R., & Andrade E Silva, M. L. (2013). In vitro antiparasitic activity and chemical composition of the essential oil obtained from the fruits of piper cubeba. Planta Medica, 79(17), 1653–1655.
Estevam, E. B. B., Silva, E. M., Miranda, M. L. D., Alves, J. M., Pereira, P. S., Silva, F. G., Esperandim, V. R., Martins, C. H. G., Ambrosio, M. A. L. V., Tófoli, D., Junior, L. R. A., & Alves, C. C. F. (2016). Avaliação das atividades antibacteriana, tripanocida e citotóxica do extrato hidroalcóolico das raízes de Tradescantia sillamontana Matuda (Veludo Branco) (Commelinaceae). Revista Brasileira de Plantas Medicinais, 18(2), 415–422.
Facundo, V. A., Rezende, C. M., & de Morais, S. M. (2003). Essential oil of zanthoxylum ekmanii (Urb.) Alain. Leaves. Journal of Essential Oil Research, 15(6), 402–403.
Lee, L. T., Garcia, S. A., Martinazzo, A. P., & Teodoro, C. E. de S. (2020). Fungitoxity and chemical composition of rosemary essential oil (Rosmarinus officinalis) on Aspergillus flavus. Research, Society and Development, 9(8), 1–16.
Lorenzi, H. (2014). Árvores Brasileiras. Manual de Identificação e Cultivo de Plantas Arbóreas Nativas do Brasil - Volume 1 (6th ed.). Instituto Plantarium de Estudo da Flora.
Moraes, T. V. de, Ferreira, J. P. G., & Moreira, R. F. A. (2020). Essential oils of the genus Pereskia: a literature review. Research, Society and Development, 9(5), 1–15.
Neves, D. P. (2011). Parasitologia humana (12th ed.). Atheneu Editor.
Pereira, S. S., Lopes, L. S., Marques, R. B., Figueiredo, K. A., Costa, D. A., Chaves, M. H., & Almeida, F. R. C. (2010). Antinociceptive effect of Zanthoxylum rhoifolium Lam. (Rutaceae) in models of acute pain in rodents. Journal of Ethnopharmacology, 129(2), 227–231.
Rey, L. (2013). Parasitologia: Parasitas e Doenças Parasitárias do Homem nos Trópicos Ocidentais. (4th ed.). Guanabara Koogan.
Santoro, G. F., Das Graças Cardoso, M., Guimarães, L. G. L., Salgado, A. P. S. P., Menna-Barreto, R. F. S., & Soares, M. J. (2007). Effect of oregano (Origanum vulgare L.) and thyme (Thymus vulgaris L.) essential oils on Trypanosoma cruzi (Protozoa: Kinetoplastida) growth and ultrastructure. Parasitology Research, 100(4), 783–790.
Silva, L. H. V. (2015). Estudo do efeito inibitório dos óleos essenciais de pimenta de macaco (Piper aduncum) e pimenta longa (Piper hispidinervum) contra Trypanosoma cruzi. Fundação Oswaldo Cruz.
Sobral, I. S., Souza-Neta, L. C., Costa, G. A. N., Guedes, M. L. S., Martins, D., & Cruz, F. G. (2009). Xantonas, triterpenos e atividade antibacteriana do extrato em diclorometano de Kielmeyera cuspidata Saddi, Clusiaceae. Brazilian Journal of Pharmacognosy, 19(3), 686–689.
WHO. (2020). Chagas disease (also known as American trypanosomiasis). World Health Organization. https://www.who.int/en/news-room/fact-sheets/detail/chagas-disease-(american-trypanosomiasis)
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Copyright (c) 2020 Silvânia de Sousa Silva; Raiane Silva Lemes ; Raphaela Gabrí Bitencourt; Vania Sardinha dos Santos Diniz; Viviane Rodrigues Esperandim ; Daniele da Silva Ferreira; Marcio Luís Andrade e Silva; Cristiane de Melo Cazal
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