Evaluación de la toxicidad biológica de los puntos cuánticos de CdTe en Trypanosoma cruzi
DOI:
https://doi.org/10.33448/rsd-v9i12.11274Palabras clave:
Parásitos protozoarios; Nanopartículas de fluorescencia; Bioimagen; Nanotoxicidad.Resumen
Los nanocristales semiconductores luminiscentes o puntos cuánticos (QD) emergen como importantes sondas fluorescentes para estudios in vitro e in vivo con células de Trypanosoma cruzi. Sin embargo, para garantizar la aplicabilidad a los organismos vivos, aún deben realizarse varias pruebas. Dado que varios eventos tóxicos son causados por QD, como la pérdida del potencial de la membrana mitocondrial, la generación de ROS, el daño del ADN y la muerte celular por autofagia. Realizamos una revisión de la literatura sobre los mecanismos de captación celular, internalización y citotoxicidad de las nanopartículas, incluidos nuestros resultados sobre la evaluación de la toxicidad biológica en T. cruzi. Evaluamos los posibles efectos sobre las curvas de crecimiento del parásito en una escala de tiempo de control y las células incubadas con diferentes concentraciones de CdTe - QDs (0.2; 2.0; 20; 200µM) para determinar los cambios en las células de desarrollo. Además, las ROS intracelulares se midieron mediante la técnica de espectroscopia de resonancia paramagnética electrónica (EPR). Según nuestros resultados, podemos inferir que los efectos tóxicos de las QD en T. cruzi son dosis-dependientes y que los niveles altos de ROS están implicados en la toxicidad celular promovida por concentraciones más altas de QD. En resumen, los parásitos etiquetados con bajas concentraciones de nanopartículas son adecuados y pueden usarse como herramientas de bioimagen para parásitos vivos. Sin embargo, es necesario realizar más estudios sobre citotoxicidad QD.
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Derechos de autor 2020 Graziella Santos Martins; Suzete Araújo Oliveira Gomes; Sônia Renaux Wanderley Louro; Eliane Wajnberg; Odivaldo Cambraia Alves; Diogo Burigo Almeida; Carlos Lenz Cesar; Denise Feder
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