In vitro maturation of domestic cat oocytes subjected to different incubation times

Denilsa Pires  Fernandes; Fernanda Araujo dos  Santos; Luã Barbalho de  Macêdo; Roberta Gonçalves  Izzo; Brenna de Sousa  Barbosa; Muriel Magda Lustosa  Pimentel; Alexsandra Fernandes  Pereira; Marcelo Barbosa  Bezerra

doi:10.33448/rsd-v10i3.13074

Autores/as

Denilsa Pires Fernandes Federal Rural University of the Semi-Arid https://orcid.org/0000-0002-3090-6853
Fernanda Araujo dos Santos Federal Rural University of the Semi-Arid https://orcid.org/0000-0001-6972-7492
Luã Barbalho de Macêdo Federal Rural University of the Semi-Arid https://orcid.org/0000-0002-8274-8797
Roberta Gonçalves Izzo Federal Rural University of the Semi-Arid https://orcid.org/0000-0003-3355-2111
Brenna de Sousa Barbosa Universidade Federal Rural do Semi-Árido https://orcid.org/0000-0002-3090-6853
Muriel Magda Lustosa Pimentel Cesmac University Center https://orcid.org/0000-0002-3498-4090
Alexsandra Fernandes Pereira Federal Rural University of the Semi-Arid https://orcid.org/0000-0003-2137-854X
Marcelo Barbosa Bezerra Federal Rural University of the Semi-Arid https://orcid.org/0000-0001-7368-5993

DOI:

https://doi.org/10.33448/rsd-v10i3.13074

Palabras clave:

Felino domestico; Primer corpúsculo polar; Maduración; Competencia meiótica.

Resumen

El objetivo de este estudio fue evaluar el efecto de tres tiempos de incubación diferentes sobre la maduración in vitro de ovocitos de gatos domésticos. Así, los ovarios (n = 42) se sometieron al procedimiento de corte y se clasificaron los ovocitos recuperados; solo ovocitos de buena calidad (grados I y II) se sometieron a maduración in vitro durante tres períodos diferentes (24 frente a 30 frente a 36 h) en medio TCM-99 suplementado. Luego, se evaluaron los ovocitos para determinar la expansión de las células del cúmulo y la presencia del primer cuerpo polar. Después de seis repeticiones (7 ± 1,7 por repetición), se recuperó un total de 334 ovocitos viables. Se observaron diferencias (p <0,05) en relación al porcentaje de ovocitos que presentan expansión celular de cúmulos, donde se observaron valores más altos en el grupo de ovocitos incubados por 36 h (84,3%), comparado con 30 (73,4 %) y 24h (71,0%). También se observaron diferencias en relación a la presencia del primer cuerpo polar (24 h: 29,7%; 30 h: 58,2%; 36 h: 69,8%). Concluimos que el período de incubación influyó en las tasas de maduración, indicando 36 h como el período ideal para la maduración in vitro de ovocitos de gatos domésticos en medio TCM-199 suplementado.

Citas

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Maduración in vitro de ovocitos de gato doméstico sometidos a diferentes tiempos de incubación

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