Asociación de compuestos de colágeno marino/biosilicato y fotobiomodulación en el proceso de cicatrización ósea utilizando un modelo experimental de defecto de calvaria
DOI:
https://doi.org/10.33448/rsd-v10i8.16498Palabras clave:
Fotobiomodulación; Biomateriales; Ingeniería de tejidos.Resumen
El estudio comparó la capacidad de regeneración ósea en un modelo experimental de defectos óseos craneales en ratas, en 3 grupos: G1: defectos óseos irradiados con fotobiomodulación; G2: Biosilicato + fotobiomodulación y G3: Biosilicato y Spongin + fotobiomodulación. La histocompatibilidad y las respuestas óseas se realizaron a los 15 y 45 días de la implantación. El análisis histológico demostró que los animales irradiados con fotobiomodulación presentaron una mayor cantidad de recién formados con el tiempo. La histomorfometría mostró valores más altos para el volumen óseo para G3 y G1, valores más altos para el volumen osteoide y el número de osteoblastos observados para G3 en comparación con G2. El inmunomarcaje de TGF-β fue mayor para G2. Los valores encontrados para VEGF fueron mayores para el biosilicato (con o sin esponja) a los 15 días de la implantación, observándose una mayor diferencia para G1, 45 días después de la cirugía. En conclusión, el estímulo proporcionado por la fotobiomodulación asociada al compuesto biomimético aumentó la formación de hueso en el defecto óseo craneal en ratas. En consecuencia, estos datos destacan el potencial de la introducción de espongina en biosilicato e irradiado con fotobiomodulación para mejorar el rendimiento biológico para aplicaciones de regeneración ósea.
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Derechos de autor 2021 Giovanna Caroline Aparecida do Vale; Kelly Rossetti Fernandes; Julia Risso Parisi; Alan de França Santana; Matheus de Almeida Cruz; Carlos Alberto Fortulan; Edgar Dutra Zanotto; Oscar Peitl; Renata Neves Granito; Ana Claudia Muniz Rennó
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