Evaluación del desarrollo de Staphylococcus aureus durante la producción de suero en polvo a escala de laboratorio
DOI:
https://doi.org/10.33448/rsd-v11i10.22450Palabras clave:
Suero; Evaporación; Cristalización; Secado; Biopelícula.Resumen
El objetivo de este estudio fue evaluar la multiplicación de S. aureus durante la producción de suero en polvo a escala de laboratorio. El suero se inoculó con 106 UFC ∙ mL-1 del cultivo mixto de S. aureus al inicio del procesamiento y se evaluó la persistencia del microorganismo después de la pasteurización, evaporación al vacío y secado. También se evaluó la recontaminación del suero por biopelículas. Se encontró una población de 102 UFC ∙ mL-1 de S. aureus después de la evaporación al vacío. El microorganismo pudo multiplicarse en el suero concentrado durante la cristalización de la lactosa, lo que infiere que es posible formar una biopelícula durante esta etapa. El análisis de microscopía confocal confirmó la presencia de células adheridas después de 4 h de proceso (103 UFC ∙ cm2). Después de 12 h de incubación, el número de células adheridas fue de aproximadamente 105 UFC ∙ cm2. Las células sésiles de S. aureus en acero inoxidable pudieron volver a la fase planctónica. Después del secado por aspersión, se verificó que la población de S. aureus en el polvo era de 103 UFC ∙ g-1. Los resultados obtenidos a escala de laboratorio apuntan a la necesidad de sistemas de aseguramiento de la calidad para controlar los pasos de la producción industrial de suero en polvo.
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