Bentonita antimicrobiana mediante la adición de acetato de geranilo para la adsorción de aflatoxina B1

Autores/as

DOI:

https://doi.org/10.33448/rsd-v11i2.25647

Palabras clave:

Bentonita; Acetato de geranilo; Actividad antimicrobiana; Adsorción de Aflatoxina B1.

Resumen

Se preparó un nanocompuesto a base de bentonita organófila (BO) y acetato de geranilo (AG) en agitador durante 1 hora, 180 rpm y temperatura ambiente, siendo posteriormente secado en estufa a 90 ºC±2 ºC durante 48 horas. Luego, este material se caracterizó por su actividad antibacteriana contra Staphylococcus aureus y Escherichia coli y antifúngica contra Aspergillus flavus y Aspergillus niger, respectivamente. Además, se evaluó la capacidad de adsorción de BO y BO/AG utilizando Aflatoxina B1 en la concentración de 107 µg∙L-1. Después de 17 h, el compuesto eliminó una cantidad significativa de micotoxinas, estando por debajo de 20 µg∙L-1. Los resultados mostraron que este compuesto tiene una buena capacidad de adsorción, puede ser eficaz en la eliminación de micotoxinas en medios acuosos y una excelente actividad antibacteriana y antifúngica.

Citas

ASTM. (2015). ASTM G21-15 Standard Practice for Determining Resistance of Synthetic Polymeric Materials to Fungi. Americam Society For Testing And Materials – ASTM.

Brasil. (1988). Ministério da Agricultura, Pecuária e Abastecimento – MAPA. Portaria n.º 7, de 9 de novembro de 1988. Fixa padrões de tolerância para aflatoxinas em alimentos para consumo animal: matérias primas e rações. Diário Oficial da União, Brasília.

Carraro, A., De Giacomo, A., Giannossi, M. L., Medici, L., Muscarella, M., Palazzo, L., Quaranta, V., Summa, V. & Tateo, F. (2014). Clay minerals as adsorbents of aflatoxin M1 from contaminated milk and effects on milk quality. Applied Clay Science, 88–89, 92-99. https://doi.org/10.1016/j.clay.2013.11.028.

CLSI. (2012). Performance standards for antimicrobial disk susceptible tests; Approved standard – Eleventh Edition. CLSI - Clinical and Laboratory Standards Institute document M02-A11. Wayne, Pennsylvania.

Fiori, M. A., Paula, M. M. S., Bernardin, A. M., Riella, H. G. & Angioletto, E. (2009). Bactericide glasses developed by Na+/Ag+ ionic exchange. Materials Science and Engineering: C, 29, 1569-1573. https://doi.org/10.1016/j.msec.2008.12.014.

Gan, F., Hang, X., Huang, Q. & Deng, Y. (2019). Assessing and modifying China bentonites for aflatoxin adsorption. Applied Clay Science, 168, 348–354. https://doi.org/10.1016/j.clay.2018.12.001.

Gonçalves, M. J., Cruz, M. T., Tavares, A. C., Cavaleiro, C., Lopes, M. C., Canhoto, J. & Salgueiro, L. (2012). Composition and biological activity of the essential oil from Thapsia minor, a new source of geranyl acetate. Industrial Crops and Products, 35, 166– 171. https://doi.org/10.1016/j.indcrop.2011.06.030.

Gupta, A., Dhakate, S. R., Pahwa, M., Sinha, S., Chand, S. & Mathur, R. B. (2013). Geranyl acetate synthesis catalyzed by Thermomyces lanuginosus lipase immobilized on electrospun polyacrylonitrile nanofiber membrane. Process Biochemistry, 48, 124-132. https://doi.org/10.1016/j.procbio.2012.09.028.

Li, Y., Tian, G., Dong, G., Bai, S., Han, X., Liang, J., Meng, J. & Zhang, H. (2018). Research progress on the raw and modified montmorillonites as adsorbents for mycotoxins: A review. Applied Clay Science, 163, 299–311. https://doi.org/10.1016/j.clay.2018.07.032.

Liu, J. H., Cai, W. K., Khatoon, N., Yu, W. H. & Zhou, C. H. (2021). On how montmorillonite as an ingredient in animal feed functions. Applied Clay Science, 202, 1-16. https://doi.org/10.1016/j.clay.2020.105963.

Macuvele, D. L. P., Nones, J., Matsinhe, J. V., Bezerra, A. T., Lima, M. M., Santos, E. S. W., Fiori. M. A. & Riella, H. G. (2017). Simultaneous effect of reaction temperature and concentration of surfactant in organo-bentonite synthesis. Materials Science Forum, 899, 42-47. https://doi.org/10.4028/www.scientific.net/MSF.899.42.

Magnoli, A. P., Tallone, L., Rosa, C. A. R., Dalcero, A. M., Chiacchiera, S. M. & Sanchez, R. M. T. (2008). Commercial bentonites as detoxifier of broiler feed contaminated with aflatoxin. Applied Clay Science, 40 (1-4), 63–71. https://doi.org/10.1016/j.clay.2007.07.007.

Muñoz-Bonilla, A. & Fernández-García, M. (2012). Polymeric materials with antimicrobial activity. Progress in Polymer Science, 37 (2), 281-339. https://doi.org/10.1016/j.progpolymsci.2011.08.005.

Nones, J., Nones, J. & Scussel, V. M. (2014). Analysis of the presence of mycotoxins in swine feed and its possible effects on semen quality in a rural property of Santa Catarina. Revista de Ciências Agroveterinárias, 13 (1), 7-13. https://revistas.udesc.br/index.php/agroveterinaria/article/view/5168/3355.

Oplatowska-Stachowiak, M., Sajic, N., Xu, Y., Haughey, S. A., Mooney, M. H., Gong, Y.Y., Verheijen, R. & Elliott, C. T. (2016). Fast and sensitive aflatoxin B1 and total aflatoxins ELISAs for analysis of peanuts, maize and feed ingredients. Food Control. 63, 239-245. http://dx.doi.org/10.1016/j.foodcont.2015.11.041.

Pereira, C. S., Cunha, S. C. & Fernandes, J. O. (2019). Prevalent Mycotoxins in Animal Feed: Occurrence and Analytical Methods. Toxins, 11 (5), 1-62. https://doi.org/10.3390/toxins11050290.

Raiola, A., Tenore, G. C., Manyes, L., Meca, G. & Ritieni, A (2015). Risk analysis of main mycotoxins occurring in food for children: An overview. Food and Chemical Toxicology, 84, 169-180. https://doi.org/10.1016/j.fct.2015.08.023.

Vila-Donat, P., Marín, S., Sanchis, V. & Ramos, A. J. (2019). New mycotoxin adsorbents based on tri-octahedral bentonites for animal feed. Animal Feed Science and Technology, 255, 1-13. https://doi.org/10.1016/j.anifeedsci.2019.114228.

Zain, M. E. (2011). Impact of mycotoxins on humans and animals. Journal of Saudi Chemical Society, 15 (2), 129-144. https://doi.org/10.1016/j.jscs.2010.06.006.

Zeferino, R. C. F., Piaia, V. A. A., Orso, V. T., Pinheiro, V. M., Zanetti, M., Colpani, G. L., Padoin, N., Soares, C., Fiori, M. A. & Riella, H. G. (2021). Synthesis of geranyl acetate by esterification of geraniol with acetic anhydride through heterogeneous catalysis using ion exchange resin. Chemical Engineering Research and Design, 168, 156-168. https://doi.org/10.1016/j.cherd.2021.01.031.

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Publicado

20/01/2022

Cómo citar

CAPELEZZO, A. P.; CELUPPI, L. C. M. .; CAMERINI, T.; MACUVELE, D. L. P. .; ZEFERINO, R. C. F. .; MELLO, J. M. M. de .; RIELLA, H. G. .; FIORI, M. A. Bentonita antimicrobiana mediante la adición de acetato de geranilo para la adsorción de aflatoxina B1 . Research, Society and Development, [S. l.], v. 11, n. 2, p. e12411225647, 2022. DOI: 10.33448/rsd-v11i2.25647. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/25647. Acesso em: 27 jul. 2024.

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Sección

Ingenierías