HA1077 inhibits cell proliferation of oral squamous cell carcinoma in vitro

Guilherme Henrique Borges; Simone de Sales Costa Moreira  Carboni; Anna Cecília Dias Maciel  Carneiro; Lorraine Stephanie  Hiss; Isadora Caixeta da Silveira; Virginia Oliveira Crema

doi:10.33448/rsd-v11i3.26730

Autores/as

Guilherme Henrique Borges Federal University of Triângulo Mineiro https://orcid.org/0000-0003-4086-0534
Simone de Sales Costa Moreira Carboni Federal University of Triângulo Mineiro https://orcid.org/0000-0003-1329-7504
Anna Cecília Dias Maciel Carneiro Federal University of Triângulo Mineiro https://orcid.org/0000-0003-4865-1286
Lorraine Stephanie Hiss Federal University of Triângulo Mineiro https://orcid.org/0000-0003-3872-287X
Isadora Caixeta da Silveira Federal University of Triângulo Mineiro https://orcid.org/0000-0002-7723-2059
Virginia Oliveira Crema Federal University of Triângulo Mineiro https://orcid.org/0000-0001-5219-532X

DOI:

https://doi.org/10.33448/rsd-v11i3.26730

Palabras clave:

Proliferación celular; HA-1077; Carcinoma oral de células escamosas; ROCKs; SCC-4; Y-27632.

Resumen

El carcinoma oral de células escamosas es una lesión maligna común. Las proteínas ROCKs están asociadas con la patogénesis y progresión de tumores humanos. Este estudio tuvo como objetivo evaluar el papel funcional de las ROCK en la regulación de la proliferación celular del carcinoma oral de células escamosas in vitro. Los ensayos de incorporación de BrdU y la inmunoexpresión de KI-67 se realizaron utilizando la línea celular SCC-4 de carcinoma de células escamosas orales. Células control y tratadas: HA-1077 (25, 50 y 100 μmol/l), 50 μmol HA-1077 y Y-27632 30 μmol/l, Y-27632 30 μmol/l se cultivaron durante 6 h. El número de células SCC-4 tratadas con: HA-1077 (25, 50 y 100 μmol/l), HA-1077 50 μmol/l y/o Y-27632 30 μmol/l fue significativamente menor que las células de control en el ensayo BrdU [F (5,17) = 443.818, p<0,0001] y en ensayo KI-67 [F = 192.595, df = 5,17; p<0,0001]. Los resultados obtenidos sugieren que las vías que evolucionan las proteínas ROCKs juegan un papel funcional importante en la regulación positiva de la proliferación celular en el carcinoma de células escamosas oral.

Citas

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HA1077 inhibe la proliferación celular en el carcinoma oral de células escamosas in vitro

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