Physico-chemical and microbiological characterization of the “saburá” from Jandaíra bee (Melipona subnitida)
DOI:
https://doi.org/10.33448/rsd-v11i7.30250Keywords:
Meliponini; Fermentation; Bee pollen; Legislação.Abstract
The fermented pollen from stingless bees (saburá) has been gaining prominence in brazilian national market due to its nutritional value, medicinal properties and unique flavor. However, the physicochemical and microbiological characteristics of this product remain unknown, since each species of stingless bee produces a different variety of saburá and there are a few studies carried out about it. The objective of this work was to determine the physicochemical and microbiological characteristics of the Jandaíra bee saburá (Melipona subnitida) from the semiarid region of the state of Rio Grande do Norte. Six samples of jandaíra bee saburá were collected in meliponaries in rural and urban areas of Mossoró city, Brazil. Microbiological analyzes of total mesophilic bacteria, total coliforms and Escherichia coli, Staphylococcus sp., Salmonella spp., molds and yeasts and lactic acid bacteria were performed. In addition, the samples were submitted to physical-chemical analysis of dry matter, mineral matter (ash), crude protein, ether extract, total sugars and neutral detergent fibers (fibers), pH and acidity of the samples. There was no detection of microorganisms in the analyzed samples. This could be an indication that the biochemical and microbial processes managed by bees are efficient in inhibiting the deterioration of this product. As for the physical-chemical parameters, the saburá analyzed presented average moisture contents of 27.2 g/100 g; crude protein 31.1 g/100 g; total sugars 55.2 g/100 g; pH 3.5 and acidity 1023.7 mEq/kg. Thus, the jandaíra bee saburá proved to be a food rich in proteins and carbohydrates and safe for human consumption.
References
Alves, R. M. O. & Carvalho, C. A. L. (2018). Pot-Pollen ‘Samburá’ Marketing in Brazil and Suggested Legislation. In P. Vit, S. R. M. Pedro, & D. W. Roubik (Orgs.), Pot-Pollen in Stingless Bee Melittology (p. 435–443). Springer International Publishing. https://doi.org/10.1007/978-3-319-61839-5_31
Alves, R. M. O., Sodré, G. S. & Carvalho, C. A. L. (2018). Chemical, Microbiological, and Palynological Composition of the “Samburá” Melipona scutellaris Pot-Pollen. In P. Vit, S. R. M. Pedro, D. W. Roubik (Orgs.), Pot-Pollen in Stingless Bee Melittology (p. 349–360). Springer International Publishing. https://doi.org/10.1007/978-3-319-61839-5_25
Anderson, K. E., Carroll, M. J., Sheehan, T., Mott, B. M., Maes, P. & Corby-Harris, V. (2014). Hive-stored pollen of honey bees: Many lines of evidence are consistent with pollen preservation, not nutrient conversion. Molecular Ecology, 23(23), 5904–5917. https://doi.org/10.1111/mec.12966
Brasil. Ministério da Agricultura, Pecuária e Abastecimento. (2001). Instrução normativa nº 3, de 19 de janeiro de 2001. Diário Oficial da União, nº 16, terça-feira, 23 de jan. de 2001.
Campos, M. G., Anjos, O., Chica, M., Campoy, P., Nozkova, J., Almaraz-Abarca, N., Barreto, L. M. R. C., Nordi, J. C., Estevinho, L. M., Pascoal, A., Paula, V. B., Chopina, A., Dias, L. G., Tešić, Ž. L. j., Mosić, M. D., Kostić, A. Ž., Pešić, M. B., Milojković-Opsenica, D. M., Sickel, W. & Carreck, N. L. (2021). Standard methods for pollen research. Journal of Apicultural Research, 60(4), 1–109. https://doi.org/10.1080/00218839.2021.1948240
Costa, M. C. A., Morgano, M. A., Ferreira, M. M. C., & Milani, R. F. (2017). Analysis of bee pollen constituents from different Brazilian regions: Quantification by NIR spectroscopy and PLS regression. LWT, 80, 76–83. https://doi.org/10.1016/j.lwt.2017.02.003
Dantas, M. C. A. M., Batista, J. de L., Dantas, P. A. M., Dantas, I. M., Dias, V. H. P., Andrade Filho, F. C., Moreira, J. N., Mielezrski, G. L. N., Silva, M. G., Maia, A. G., Medeiros, A. C. & Maracajá, P. B. (2020). Stingless bee and its socioeconomic potential in the States of Paraíba and Rio Grande do Norte. Research, Society and Development, 9(10), e3309107939. https://doi.org/10.33448/rsd-v9i10.7939
De Man, J. C., Rogosa, M. & Sharpe, M. E. (1960). A Medium for the Cultivation of Lactobacilli. Journal of Applied Bacteriology, 23(1), 130–135. https://doi.org/10.1111/j.1365-2672.1960.tb00188.x
Fernández, L. A., Rodríguez, M. A., Sánchez, R. M., Pérez, M. & Gallez, L. M. (2021). Long-term microbiological and chemical changes in bee pollen for human consumption: Influence of time and storage conditions. Journal of Apicultural Research, 60(2), 319–325. https://doi.org/10.1080/00218839.2020.1728867
Isik, A., Ozdemir, M. & Doymaz, I. (2021). Investigation of microwave drying on quality attributes, sensory properties and surface structure of bee pollen grains by scanning electron microscopy. Brazilian Journal of Chemical Engineering, 38(1), 177–188. https://doi.org/10.1007/s43153-020-00088-w
International Organization for Standardization. (2007) Microbiology of food and animal feeding stuffs: Horizontal method for the detection of Salmonella spp. (ISO 6579:2002/AMD 1:2007). https://www.iso.org/standard/42109.html
Instituto Adolfo Lutz (2008). Métodos físico-químicos para análises de alimentos. São Paulo: Instituto Adolfo Lutz, 1020p.
Kieliszek, M., Piwowarek, K., Kot, A. M., Błażejak, S., Chlebowska-Śmigiel, A. & Wolska, I. (2018). Pollen and bee bread as new health-oriented products: A review. Trends in Food Science & Technology, 71, 170–180. https://doi.org/10.1016/j.tifs.2017.10.021
Kostić, A. Ž., Milinčić, D. D., Barać, M. B., Ali Shariati, M., Tešić, Ž. Lj. & Pešić, M. B. (2020). The Application of Pollen as a Functional Food and Feed Ingredient—The Present and Perspectives. Biomolecules, 10(1). https://doi.org/10.3390/biom10010084
Manafi, M. & Kneifel, W. (1989). A combined chromogenic-fluorogenic medium for the simultaneous detection of coliform groups and E. coli in water. Zentralbl Hyg Umweltmed, 189(3), 225–234.
Maturin, L. & Peeler, J. T. (1998). Aerobic Plate Count. In: Bacteriological Analytical Manual Online.
Melo, A. A. M., Estevinho, M. L. M. F., Sattler, J. A. G., Souza, B. R., Freitas, A. S., Barth, O. M. & Almeida-Muradian, L. B. (2016). Effect of processing conditions on characteristics of dehydrated bee-pollen and correlation between quality parameters. LWT - Food Science and Technology, 65, 808–815. https://doi.org/10.1016/j.lwt.2015.09.014
Menezes, C., Vollet-Neto A., Contrera, F.A.F.L., Venturieri, G.C. & Imperatriz-Fonseca, V.L. (2013) The Role of Useful Microorganisms to Stingless Bees and Stingless Beekeeping. In P. Vit, S. R. M. Pedro, & D. W. Roubik. (Orgs.), Pot-Honey. (p. 153–171). Springer International Publishing. https://doi.org/10.1007/978-1-4614-4960-7_10
Mohammad, S. M., Mahmud-Ab-Rashid, N.-K. & Zawawi, N. (2021). Stingless Bee-Collected Pollen (Bee Bread): Chemical and Microbiology Properties and Health Benefits. Molecules, 26(4). https://doi.org/10.3390/molecules26040957
Nogueira-Neto, P. (1997). Vida e criação de abelhas indígenas sem ferrão. São Paulo: Editora Nogueirapis, 446p.
Oliveira, J. J., & Ribeiro, H. (2020). Food market trends: the cases of spirulina and bee pollen. In: Ribeiro, H. N. R., Costa, M. A. S. & Cehok, I. (ed.). Economic and Social Development. Aveiro: Varazdin Development And Entrepreneurship Agency, p. 246-258.
Parpinelli, R. S., Lima, E. G., Anjo, F. A., Almeida, L. M., Março, P. H. & Toledo, V. A. A. (2021). Microbiological characteristics of meliponine honey marketed in the State of Paraná – Brazil. Research, Society and Development, 10(1), e6710111381. https://doi.org/10.33448/rsd-v10i1.11381
Pinheiro, F. M., Costa, C. V. P. N., Baptista, R. C.; Venturieri, G. C. & Pontes, M. A. N. (2007). Pólen de abelhas indígenas sem ferrão Melipona fasciculata e Melipona flavolineata: caracterização físico-química, microbiológica e sensorial. In: Encontro de profissionais da química da amazônia, 10., 2007, Belém, PA. Recursos naturais: uma reflexão para os profissionais da química. Belém, PA: Conselho Regional de Química da 6ª Região.
Rodrigues, C. S., Ferasso, D. C., Prestes, O. D., Zanella, R., Grando, R. C., Treichel, H., Coelho, G. C. & Mossi, A. J. (2018). Quality of Meliponinae honey: Pesticides residues, pollen identity, and microbiological profiles. Environmental Quality Management, 27(4), 39–45. https://doi.org/10.1002/tqem.21547
Silva, T. M. S., Camara, C. A., Lins, A. C. S., Barbosa-Filho, J. M., Silva, E. M. S., Freitas, B. M. & Santos, F. A. R. (2006). Chemical composition and free radical scavenging activity of pollen loads from stingless bee Melipona subnitida Ducke. Biodiversity and nutrition: a common path, 19(6), 507–511. https://doi.org/10.1016/j.jfca.2005.12.011
Silva, T. M. S., Santos, F. P., Evangelista-Rodrigues, A., Silva, E. M. S., Silva, G. S., Novais, J. S., Santos, F. A. R. & Camara, C. A. (2013). Phenolic compounds, melissopalynological, physicochemical analysis and antioxidant activity of jandaíra (Melipona subnitida) honey. Journal of Food Composition and Analysis, 29(1), 10–18. https://doi.org/10.1016/j.jfca.2012.08.010
Tallent, S., Hait, J., Bennett, R.W. & Lancette G.A. (1998). Staphylococcus aureus. In: Bacteriological Analytical Manual Online. https://www.fda.gov/food/laboratory-methods-food/bam-chapter-12-staphylococcus-aureus Acesso em: 21 de outubro de 2020.
Thakur, M. & Nanda, V. (2020). Composition and functionality of bee pollen: A review. Trends in Food Science & Technology, 98, 82–106. https://doi.org/10.1016/j.tifs.2020.02.001
Tournas, V., Stack, M.E., Mislivec, P.B., Koch, H.A. & Bandler, R. (1998). Yeasts, molds and mycotoxins. In: Bacteriological Analytical Manual Online. Disponível em: https://www.fda.gov/food/laboratory-methods-food/bam-chapter-18-yeasts-molds-and-mycotoxins Acesso em: 21 de outubro de 2020.
Villas-Bôas, J. K. (2018). Manual de Aproveitamento Integral dos Produtos das Abelhas sem Ferrão. (2a ed.), Instituto Sociedade, População e Natureza, 212 p.
Vit, P., Bertha, S., Silvia, P., Ruíz, J., Maza, F., María, P. & Elizabeth, P. (2016). Chemical And Bioactive Characterization Of Pot-Pollen Produced By Melipona And Scaptotrigona Stingless Bees From Paria Grande, Amazonas State, Venezuela. Emirates Journal of Food And Agriculture, 28(2), 78-84. 10.9755/ejfa.
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Copyright (c) 2022 Kewen Santiago da Silva Luz; Thamirys Lorranne Santos Lima; Joice Teixeira Souza; Maria Rociene Abrantes; Palloma Vitória Carlos de Oliveira; Érica Lorenna Batista da Silva ; João Batista Pinheiro Filho; Pâmara Virna Carlos de Oliveira; Luiz Fernando Bezerra Evangelista; Jean Berg Alves da Silva
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