Evaluación de dos modelos experimentales de trombosis venosa profunda: estasis venosa y lesión endotelial
DOI:
https://doi.org/10.33448/rsd-v12i4.40975Palabras clave:
Trombosis venosa; Modelos de animales; Cloruro férrico; Ligadura.Resumen
La trombosis venosa profunda es una importante causa de morbimortalidad en el mundo, especialmente en el ámbito hospitalario, justificando su tratamiento y también profilaxis cuando sea necesario. Los métodos de inducción de trombosis venosa profunda en animales son fundamentales para el estudio de la fisiopatología de la enfermedad, así como para el ensayo de fármacos antitrombóticos. Los objetivos del estudio fueron evaluar y comparar dos métodos de inducción de trombosis venosa profunda en ratas: el modelo de estasis venosa, ampliamente descrito en la literatura, y el modelo de lesión endotelial, con pocos estudios, principalmente en ratas. Se utilizaron ratas Wistar macho para inducir trombosis venosa profunda. Para la estasis, se disecó y ligó la vena cava inferior durante 3 horas, después de lo cual se extrajo el segmento con la vena que contenía el trombo. Para el modelo de lesión endotelial, se aplicó un trozo de papel de filtro empapado en FeCl3 durante 1 minuto y se evaluó el segmento después de 1 hora. En ambos modelos se evaluó el peso húmedo y el área de oclusión. El peso húmedo se obtuvo comparando los métodos de lesión endotelial y estasis respectivamente: 17,7 mg (±3,0 mg) vs. 2,34 mg (±1,8 mg) con P<0,001. Para el área de oclusión, comparando los métodos de lesión endotelial y estasis respectivamente: 85,11% (±9,67%) vs. 40,83% (±33,14%), con P<0,05. En todas las variables, el método de lesión endotelial tuvo mejores resultados que el método de estasis venosa, demostrando ser más reproducible.
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Derechos de autor 2023 Andrezza Janine de Almeida Santos; Carlos Eduardo Rodrigues Lopes; Luiz Eduardo Alessio Junior; Neiva Pereira Paim; Keila Fernanda da Cruz Souza Pinto; Rodolfo Cassimiro Araújo Berber; Aline Morandi Alessio
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