La terapia con láser de baja potencia estimula la reparación de tejidos en la articulación temporomandibular de ratas obesas sometidas a movimientos de ortodoncia
DOI:
https://doi.org/10.33448/rsd-v14i2.48199Palabras clave:
Láserterapia; Obesidad; Movimiento ortodóncico; Trastornos temporomandibulares.Resumen
El objetivo del presente estudio fue evaluar los efectos de la terapia con láser de baja potencia sobre los cambios histológicos en la articulación temporomandibular causados por el movimiento dental inducido en ratas obesas. Se utilizaron cuarenta crías de rata Wistar macho, treinta crías recibieron inyecciones subcutáneas en la región cervical de 4g/kg/día de glutamato monosódico (animales obesos - MSG) durante los primeros cinco días de vida y diez recibieron inyecciones de solución salina hiperosmótica, 1. 25g/Kg/día (animales control - CTL). Se formaron cuatro grupos experimentales (n=10/grupo): Grupo 1: Control (CTL), ratas que no fueron sometidas a ningún procedimiento experimental; Grupo 2: ratas obesas sin movimiento dental inducido (MSG); Grupo 3: ratas obesas a las que se les realizó movimiento dental inducido (MDI) (MSG + MDI); Grupo 4: ratas obesas a las que se les realizó MDI y TLBP (MSG + MDI+ TLBP). A los 90 días se instaló el dispositivo de movimiento dental inducido (MDI) en los grupos 3 y 4. A los 91 días se inició el tratamiento con el láser de baja potencia. A los 97 días de experimento, los animales fueron pesados, medidos y sacrificados. Se extirparon, fijaron y procesaron para histología las articulaciones temporomandibulares (ATM) del lado derecho. Los animales del grupo obeso y tratados con terapia con láser de baja intensidad (MSG+MDI+TLBP) mostraron una reducción en el grosor del disco articular e hiperplasia y vascularización de la membrana sinovial de la ATM. Concluimos que la terapia con láser de baja intensidad es eficaz en el tratamiento de los cambios histológicos de la ATM causados por el movimiento ortodóncico en ratas con obesidad hipotalámica.
Citas
Ahlberg, J., Nikkilä, H., Könönen, M. et al. (2004). Associations of perceived pain and painless TMD-related symptoms with alexithymia and depressive mood in media personnel with or without irregular shift work. Acta Odontol. Scand. 62, 119–123.
Aimbire F, Albertini R, Pacheco M T T et al (2006) Low-level laser therapy induces dose-dependent reduction of TNFα levels in acute inflammation. Photomed Laser Surg 24:33–37.
Alves, A. C. A., Vieira, R. D. P., Leal-Junior, E. C. P. et al (2013A) Effect of low-level laser therapy on the expression of inflammatory mediators and on neutrophils and macrophages in acute joint inflammation. Arthritis Res Ther. 15, R116.
Alves, A. C., De Carvalho, P. T., Parente, M. et al. (2013B). Low-level laser therapy in different stages of rheumatoid arthritis: a histological study. Lasers Med Sci. 28, 529-36.
Barretto, S. R., De Melo, G. C., Dos Santos, J. C. et al. (2013). Evaluation of anti-nociceptive and anti-inflammatory activity of low-level laser therapy on temporomandibular joint inflammation in rodents. J Photochem Photobiol B Biol. 129, 135–142.
Bihari, V., Kesavachandran, C., Pangtey, B.S. et al. (2011). Dor musculoesquelética e seus fatores de risco associados em residentes da Região da Capital Nacional. Indiano J Occup Environ Med. 15, 59–63.
Brandelero J.S., Bonfleur M.L., Ribeiro L.A., Vanzela E.C., Nassar C.A., Nassar P.O. et al. (2012). Decreased tnf-α gene expression in periodontal ligature in msg obese rats: a possible protective effect of hypothalamic obesity against periodontal disease? Arch Oral Biol. 57, 300-306.
Carvalho, C. M., Lacerda, J. A., Neto, F. P. S. et al. (2011). Evaluation of Laser Phototherapy in the Inflammatory Process of the Rat’s TMJ Induced by Carrageenan. Photomed Laser Surg. 29, 245-54.
Castro, I. C., Rosa, C B., Carvalho, C. M. et al. (2015). Assessment of different energy delivery settings in laser and LED phototherapies in the inflammatory process of rat's TMJ induced by carrageenan. Lasers Med Sci. 30, 2105-13.
Christidis, N., Lindström, N. E., Sandberg, A., & Tsilingaridis, G. (2019). Prevalence and treatment strategies regarding temporomandibular disorders in children and adolescents: a systematic review. J Oral Rehabil. 46, 291–301.
Colaianni G., Brunetti G., Faienza M. F., Colucci S. & Grano M. (2014). Osteoporosis and obesity: Role of Wnt pathway in human and murine models. World J Orthop. 5, 3, 242-246.
Cominato, L., Di Biagio, G. F., Lellis, D. et al. (2018). Obesity prevention: strategies and challenges in Latin America. Current Obesity Reports. 7, 97-104.
Costa, K., Bonfleur, M., Pontillo, V., Pedrotti, S., Bernardon, P., Brancalão, R., Beu, C., Nassar, P., & Nassar, C. (2017). Evaluation of Periodontal Tissues and Abdominal Aorta of Rats with Induced Obesity by Monosodium Glutamate and Experimental Periodontitis. British Journal of Medicine And Medical Research. 19, 1-14.
De Leeuw, R., & Klasser, G. (2013). Orofacial pain: guidelines for assessment, diagnosis, and management (5th edition), Quintessence Publishing Co, New York, p. 312.
Desiderá, A. C., Nascimento, G. C., Gerlach, R. F., & Leite-Panissi, C. R. A. (2015). Laser therapy reduces gelatinolytic activity in the rat trigeminal ganglion during temporomandibular joint inflammation. Oral Dis. 21, 652–658.
Detamore, M. S., Orfanos, J. G., Almarza, A. J. et al (2005) Quantitative analysis and comparative regional investigation of the extracelular matrix of the porcine temporomandibular joint disc. Matrix Biol. 24, 45–57.
Evans A. L., Paggiosi M. A., Eastell R. & Walsh J. S. (2015). Bone density, microstructure and strength in obese and normal weight men and women in younger and older adulthood. J Bone Miner Res. 30, 920-928.
Faul, F., Erdfelder, E., Lang, A. G., & Buchner, A. (2007). G*Power 3: A flexible statistical power analysis program for the social, behavioral, and biomedical sciences. Behavior Research Methods. 39, 175-191.
Felson D. T, Zhang Y., Hannan M. T & Anderson J. J. (1993). Effects of weight and body mass index on bone mineral density in men and women: the Framingham study. J Bone and Min Res. 8, 567-573.
Gameiro, G. H., Nouer, D. F., Neto, J. S. P. et al. (2008). Effects of short- and long-term celecoxib on orthodontic tooth movement. Angle Orthodontist. 78, 860–865.
Garcia, G. R., Petry, T. N. P., Brandão, H. B., Rosin, M., Amorim, E. M. P., Nassar, C. A., Balbo, S. L., & Amorim, J. P. A. (2024). Maternal Obesity and Periodontitis Induce Increased of Body Weights, Alteration of the Periodontal Tissue and Decreased of Root Resorption in Female Rats Offspring. A Comparative Study. Journal of Advances in Medicine and Medical Research, 36, 85-99.
Gauer, R. L., & Semidey, M. J. (2015). Diagnosis and treatment of temporomandibular disorders. Am Fam Physician. 91, 378–386.
Gesch, D., Bernhardt, O., Alte, D. et al. (2004). Malocclusions and clinical signs or subjective symptoms of temporomandibular disorders (TMD) in adults. Results of the population-based Study of Health in Pomerania (SHIP). J. Orofac. Orthop. 65, 88–103.
Goodson, J. M., Groppo, D., Halem, S., & Carpino, E. (2009). Is obesity an Oral Bacterial Disease? Journal of Dental Research. 88, 519-523.
Heller, I. J. & Nanda, R. (1979). The effect of metabolic alteration of periodontal fibres on orthodontic tooth movement. American Journal of Orthodontics. 75, 239–257.
Hosgor, H., Bas, B., & Celenk, C. (2017). A comparison of the outcomes of four minimally invasive treatment methods for anterior disc displacement of the temporomandibular joint. Int J Oral Maxillofac Surg. S0901-5027, 31471-6.
Huang, G. J., LeResche, L., Critchlow, C. W. et al. (2002). Risk factors for diagnostic subgroups of painful temporomandibular disorders (TMD). J. Dent. Res. 81, 284–288.
Iglesias-Linares, A. & Hartsfield, J. K Jr. (2017). Cellular and Molecular Pathways Leading to External Root Resorption. J Dent Res. 96, 145-152.
Kalpakci, K. N., Willard, V .P., Wong, M. E., & Athanasiou, K. A. (2011) Na interspecies comparison of the temporomandibular joint disc. J Dent Res. 90, 193–198.
Kvaratskhelia S, & Nemsadze T. (2022). The influence of the orthodontic treatment on the development of the temporomandibular joint disorder - literature review. Georgian Med News. 331, 22-26.
Lecka-Czernik, B., Stechschulte, L. A., Czernik, P. J. & Dowling, A. R. (2015). High bone mass in adult mice with diet-induced obesity results from a combination of initial increase in bone mass followed by attenuation in bone formation; implications for hight bone mass and decreased bone quality in obesity. Mol Cell Endocrinol. 410, 35.
Lee, K. S., Jha, N., & Kim, Y. J. (2021). Risk factor assessments of temporomandibular disorders via machine learning. Sci Rep. 11, 19802.
Lemos, G. A., Batista, A. U. D., Silva, P. L. P. et al. (2020). Photobiostimulation activity of different low-level laser dosage on masticatory muscles and temporomandibular joint in an induced arthritis rat model. Lasers in Medical Science. 35, 1129–1139.
Lemos, G. A., Da Silva, P. L. P., Batista, A. U. D., & Palomari, E. T. (2018). Experimental model of temporomandibular joint arthritis: evaluation of contralateral joint and masticatory muscles. Arch Oral Biol. 95, 79–88.
Lemos, G .A., Rissi, R., De Souza, P. I .L. et al. (2016). Low-level laser therapy stimulates tissue repair and reduces the extracellular matrix degradation in rats with induced arthritis in the temporomandibular joint. Lasers Med Sci. 31, 051–1059.
Li, Y., Jacox, L. A., Little, S. H., & Ko, C. C. (2018). Orthodontic tooth movement: The biology and clinical implications. Kaohsiung J Med Sci. 34, 207-214.
Lucietto, G., Pasa, A. C., Hellen Budel Brandão, H. B., Porto, E. M., Busato, M. A., & Amorim, J. P. A. (2024). The Impact of Ovariectomy and Dental Trauma on Dental Pulp, on Tooth Movement and Internal Root Resorption: Findings from a Rat Model. Journal of Advances in Medicine and Medical Research. 36, 42-53.
Maggio, A. B., Belli, D .C., Puigdefabregas, J. W., Rizzoli, R., Farpour-Lambert, N. J., Beghetti, M. et al. (2014). High bone density in adolescents with obesity is related to fat mass and serum leptin concentrations. J Pediatr Gastroenterol Nutr. 58, 723-728.
Marcantonio, C. C., Nogueira, A. V. B., Leguizamón, N. D. P., de Molon. R. S., Lopes, M. E. S., Silva, R. C. L., Cerri, P. S., Deschner, J., & Cirelli, J. A. (2021). Effects of obesity on periodontal tissue remodeling during orthodontic movement. Am J Orthod Dentofacial Orthop. 159, 480-490.
Mattia, T. M., Leite, M. A., Nassar, P. O., Sagae, S. C., Alegre-Maller, A. C. P., Pandini, J. H., Cardoso, N., Marchiori, V., Brancalhão, R. M. C., & Nassar, C. A. (2017).The influence of obesity induced by monosodium glutamate in periodontal tissues of female Wistar rats with experimental periodontitis. American International Journal of Contemporary Research. 7, 28-40.
Mazzetto, M. O., Hotta, T. H., & Pizzo, R. C. D. A. (2010). Measurements of jaw movements and TMJ pain intensity in patients treated with GaAlAs laser. Braz Dent J. 21, 356–360.
Meikle, M. C. (2006). The tissue, cellular, and molecular regulation of orthodontic tooth movement: 100 years after Carl Sandstedt. The European Journal of Orthodontics. 28, 221-240.
Medeiros, K. A., Siqueira, B. S., Urrutia, M. A. D., Porto, E. M., Grassiolli, S., & Amorim, J. P. A. (2021). Vagotomy associated with splenectomy reduces lipid accumulation and causes kidneys histological changes in rats with hypothalamic obesity. Acta Cirúrgica Brasileira (Online). 36, p. 1.
Mendonça, C. R., Matias, Noll., Santos, A. S. A. C. et al. (2020). High prevalence of musculoskeletal pain in individuals with severe obesity: sites, intensity, and associated factors. Korean J Pain. 33, 245–257.
Motghare, V., Kumar, J., Kamate, S. et al. (2015). Association between harmful oral habits and sign and symptoms of temporomandibular joint disorders among adolescents. J Clin Diagn Res. 9, 0–8.
National Institute of Dental and Craniofacial Research (NIDCR). (2018). Facial Pain. Preprint at https:// www. nidcr. nih. gov/ resea rch/ data- stati stics/ facial- pain.
Okifuji, A. & Hare, B. D. (2015). The association between chronic pain and obesity. J. Pain Res. 8, 399–408.
Olney J. W., Adamo N.J. & Ratner A. (1971). Monosodium glutamate effects. Science, 172, 294.
Olney, J. W. (1969). Brain lesions, obesity and other disturbances in mice treated with monosodium glutamate. Science. 164, 719-721.
Pasa, A. P. B. C., Brandão, H. C. B., Busato, M. C. A., Porto, E. M., & Amorim, J. P. A. (2024A). Effects of Ovariectomy on the Structure of the Periodontium and Root Resorption During Orthodontic Tooth Movement in Rats. Journal of Advances in Medicine and Medical Research. 36, 215-224.
Pasa, A. P. B. C., Detogni, A. C., Tomin, D. S., Busato, M. C. A., Porto, E. M., & Amorim, J. P. A. (2024B). Orthodontic movement of traumatized teeth increase the velocity of tooth movement and cause external root resorption. Research, Society and Development, [S. l.], 13(10), e99131047135.
Paulis, W. D., Silva, S., Koes, B .W., & Van Middelkoop, M. (2014). Overweight and obesity are associated with musculoskeletal complaints as early as childhood: A systematic review. Obes. Rev. 15, 52–67.
Pereira A. S. et al. (2018). Metodologia da pesquisa científica. [free e-book]. Editora UAB/NTE/UFSM.
Petry, T. N., Balbo, S.L., Moi, M. B., Suemi, V., Paiva, J P., Goncalves, V. M., Teleken, J. L., Bonfleur, M L., Amorim, J P., & Nassar, C A. (2022). Obesity and Maternal Periodontal Disease do not Cause Changes in Offspring's Adult Life - Study in Rats. Journal of Advances in Medicine and Medical Research. 34, 14-25.
Salameh, E., Alshaarani, F., Abou Hamed, H., & Abou Nassar, J. (2015). Investigation of the relationship between psychosocial stress and temporomandibular disorder in adults by measuring salivary cortisol concentration: A case–control study. J. Indian Prosthodont. Soc. 15, 148.
Sayed, N., Murugavel, C., & Gnanam, A. (2014). Management of Temporomandibular Disorders with Low Level Laser Therapy. J Maxillofac Oral Surg. 13, 444- 50.
Shitsuka, R. et al. (2014). Matemática fundamental para tecnologia. (2ed.). Editora Erica.
Skog, C., Fjellner, J., Ekberg, E., & Häggman-Henrikson, B. (2019). Tinnitus as a comorbidity to temporomandibular disorders—A systematic review. J. Oral Rehabil. 46, 87–99.
Slade, G. D., Bair, E.; Greenspan, J. D. et al. (2013). Signs and symptoms of first-onset TMD and sociodemographic predictors of its development: the OPPERA prospective cohort study. J Pain. 14, e1-3.
Smith, S. M.; Sumar, B., & Dixon, K. A. (2014). Musculoskeletal pain in overweight and obese children. Int. J. Obes. (Lond.). 38, 11–15.
Song, H. S., Shin, J. S., Lee, J. et al. (2018). Association between temporomandibular disorders, chronic diseases, and ophthalmologic and otolaryngologic disorders in Korean adults: A cross-sectional study. PLoS One. 13, e0191336.
Suvan, J. E., Finer, N., & D’aiuto, F. (2018). Periodontal complications with obesity. Periodontology 2000. 78, 98–128.
Vieira, S. (2021). Introdução à bioestatística. Editora GEN/Guanabara Koogan.
Wang, D. H., Yang, M. C., Hsu, W. E. et al. (2017). Response of the temporomandibular joint tissue of rats to rheumatoid arthritis induction methods. Journal of Dental Sciences. 12, 83–90.
Wang, X. D., Kou, X. X., Mao, J. J. et al. (2012). Sustained Inflammation Induces Degeneration of the Temporomandibular Joint. J Dent Res. 91, 499-505.
Word Health Organization (2022). Obesity and overweight. https://www.who.int/en/news-room/fact-sheets/detail/obesity-and-overweight.
Zanuzo, K., Guareschi, Z .M., Detogni, A. C., Huning, L. P., Rodrigues, P. F., Porto, E. M., Grassiolli, S., & Amorim, J. P. A. (2020). Physical exercise associated with vitamin D chronic supplementation reduces kidney injury induced by monosodium glutamate. Anais da Academia Brasileira de Ciências. 92, 1-15.
Descargas
Publicado
Cómo citar
Número
Sección
Licencia
Derechos de autor 2025 João Paulo de Arruda Amorim; Janaina Ahmann Spenassatto ; Isabela Zibetti; Andyara Sepulveda Lobato da Silva
Esta obra está bajo una licencia internacional Creative Commons Atribución 4.0.
Los autores que publican en esta revista concuerdan con los siguientes términos:
1) Los autores mantienen los derechos de autor y conceden a la revista el derecho de primera publicación, con el trabajo simultáneamente licenciado bajo la Licencia Creative Commons Attribution que permite el compartir el trabajo con reconocimiento de la autoría y publicación inicial en esta revista.
2) Los autores tienen autorización para asumir contratos adicionales por separado, para distribución no exclusiva de la versión del trabajo publicada en esta revista (por ejemplo, publicar en repositorio institucional o como capítulo de libro), con reconocimiento de autoría y publicación inicial en esta revista.
3) Los autores tienen permiso y son estimulados a publicar y distribuir su trabajo en línea (por ejemplo, en repositorios institucionales o en su página personal) a cualquier punto antes o durante el proceso editorial, ya que esto puede generar cambios productivos, así como aumentar el impacto y la cita del trabajo publicado.
