Hormone protocol strategy based on the anticipation of PGF2α to improve the reproductive efficiency of fixed time embryo transfer (FTET) in dairy cattle

Authors

DOI:

https://doi.org/10.33448/rsd-v10i6.15382

Keywords:

Hormonal protocol; Fixed time embryo transfer; PGF2α; Conception rate.

Abstract

This study aimed to compare two hormonal protocols for fixed-time embryo transfer (FTET) in dairy cows based on the moment of PGF2α application. A total of 2,172 crossbred Bos indicus cows were used. According to the embryo recipient synchronization protocol, the cows were divided into two treatments: Conventional and MMSINCRO (test treatment). The Conventional protocol consisted of inserting a device containing progesterone and administering estradiol benzoate on Day 0. On Day 8, progesterone was removed and PGF2α, eCG and estradiol cypionate were applied. On Day 10, the animals received GnRH. FTET was performed on Day 17. The MMSINCRO protocol consisted of the insertion of progesterone and administration of estradiol benzoate on Day 0. On Day 7, animals received PGF2α. On Day 9, the progesterone was removed and the animals received eCG and estradiol cypionate. On Day 11, GnRH was applied. The FTET was performed on Day 18. The conception rate of the MMSINCRO treatment recipients (52%) was significantly higher than that of the Conventional protocol (33%). The qualitative evaluation of the corpus luteum showed a better quality in the MMSINCRO treatment. Thus, it was possible to conclude that for FTET in dairy cows, it is advisable to use the MMSINCRO protocol in the recipients, which was able to increase the conception rate. This finding provides an improvement in the reproductive efficiency of dairy cattle, and consequently, a greater production of milk and dairy products on dairy farms.

References

Assis, J., Ferreira, J. D., Martins, H. H., & Schneider, M. B. (2016). Cadeia produtiva do leite no brasil no contexto do comércio internacional. Revista de Ciências Empresariais da UNIPAR, 17(1), 63-93.

Ayres, H., Marques, M. O., Silva, R. C. P., Rodrigues, C. A., Ferreira, R. M, & Baruselli, P. S. (2007). Influência do uso de eCG em diferentes períodos pós parto e do escore de condição corporal na taxa de prenhez de vacas nelore inseminadas em tempo fixo. Acta Scientiae Veterinariae, 35(3): s1113.

Barreiros, T. R. R., Blaschi, W., Borsato, E. A., Ludwig, H. E., Silva, D. R. M., Seneda, M.M. (2006). Comparação das taxas de prenhez entre receptoras com corpos lúteos cavitários ou compactos após protocolos de sincronização com clorprostenol ou transferência de embriões em tempo fixo. Semina: Ciências Agrárias, 27(4), 657-664.

Baruselli, P. L., Ferreira, R. M., Sales, J. N. S., Gimenes, L. U., Sá Filho, M. F., Martins, C. M., Rodrigues, C. A., & Bó, G. A. (2011). Timed embryo transfer programs for management of donor and recipient cattle. Theriogenology, 76(9), 1583-1593.

Gaebler, E., Eigenmann, U., Bruckmaier, R., & Bleul, U. (2015). Fate of follicular ovarian cysts in early postpartum dairy cows treated with PRID/PGF or PRID/PGF plus eCG. Tierarztl Prax Ausg Grosstiere Nutztiere, 43(6), 331-9.

Gatea, A. O., Smith, M. F., Pohler, K. G., Egen, T., Pereira, M. H. C., Vasconselos, J. L. M., Lawrence, J. C., & Green, J.A. (2018). The ability to predict pregnancy loss in cattle with ELISAs that detect pregnancy associated glycoproteins is antibody dependent. Theriogenology, 108(1), 269-276.

Leite, P. A. G., Carvalho, G. R., Rodrigues, M. T., Ruas, J.R.M., Amorim, E. A. M., & Maffili, V. V. (2006). Induction of the out-of-season ovulation (with LH and GnRH) and estrus (with progesterone) in goats. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 58(3), .360-366.

Lucy, M. C. (2019). Symposium review: Selection for fertility in the modern dairy cow-Current status and future direction for genetic selection. Journal Dairy Science 102(4), 3706-3721.

Luedke, F. E., Lavach, F. L., Cassanta, F. G., Nunes, L. F. N., Schlotefeldt, C., Paiva, S. M., Santos, S. I., & Neves, A. P. (2019). Aspectos da produção in vitro de embriões bovinos no Brasil – revisão. Pesquisa agropecuária gaúcha, 25(1/2), 120-132.

Medalha, A. G., Souza, M. I. L., Souza, A. S., Sá Filho, O. G., Queiroz, V. L. D., & Costa Filho, L. C. C. (2015). Evaluation of intravaginal progesterone device, in until three uses, in fixed-time artificial insemination in "Bos indicus" females. Revista brasileira de saúde e produção animal, 16(2), 458-469.

Mee, M. O., Stevenson, J. S. & Scoby, R. K. (1990). Influence of gonadotropin-releasing hormone and timing of insemination relative to estrus on pregnancy rates of dairy cattle at fi rst service. Journal of Dairy Science, 73(6), 1500-1507.

Morais, M. E. O., Mello, R. R. C., Ferreira, J. E., & Mello, M. R. B. (2013). Comparação de diferentes métodos de manejo reprodutivo em receptoras de embrião bovino sobre a taxa de concepção. Revista Brasileira em Ciências Veterinárias, 20(2), 89-93.

Negreiros, M. P. M., Seugling, G. H. F., Almeida, A. B. M., Hidalgo, M. M. T., Martins, M. I. M., Blaschi, W., & Barreiros, T. R. R. (2020). Influence of nutritional and ovarian parameters on pregnancy rates of Nelore cows artificially inseminated at fixed time. Research, Society and Development, 9(9), e907998091.

Núñez-Olivera, R., Castro, T., García-Pintos, C., Bó, G., Piaggio, J., & Menchaca, A. (2014). Ovulatory response and luteal function after eCG administration at the end of a progesterone and estradiol' based treatment in postpartum anestrous beef cattle. Animal Reproduction Science, 146(3-4), 111-116.

Oliveira, F. A., Almeida, I. C., Penitente Filho, J. M., & Torres, C. A. A. (2020). Estradiol e GNRH na sincronização do estro e indução da ovulação de vacas mestiças. Revista Caatinga, 33(3), 815-823.

Pellegrino, C. A. G., Morotti, F., Untura, R. M., Pontes, J. H. F., Pellegrino, M. F. O., Campolina, J. P., Seneda, M. M., Barbosa, F. A., & Henry, M. (2016). Use of sexed sorted semen for fixed-time artificial insemination or fixed-time embryo transfer of in vitro produced embryos in cattle. Theriogenology, 86(1), 888-893.

Pessoa, A. B. C. M., Pereira, E. T. N., Melo, M. I. V. (2014). Influência do local de inovulação e do tamanho de corpo lúteo sobre a taxa de prenhez em programa de transferência de embriões bovinos em tempo fixo. Revista Brasileira de Reprodução Animal, 38(4), 237-241.

Pujo, M., López-Béjarb, M., & Paramio, M. T. (2004). Developmental competence of heifer oocytes selected using the brilliant cresyl blue (BCB) test. Theriogenology, 61 (4), 735-744.

Rossi, G., De Leo, G. A., Pongolini, S., Natalini, S., Zarenghi, L., Ricchi, M., & Bolzoni, L. (2017). The potential role of direct and indirect contacts on infection spread in dairy farm networks. Plos Computational Biology, 13(1), e1005301.

Sá Filho, O. G., Meneghetti, M., Peres, R. F. G., Lamb, G. C., & Vasconcelos, J. L. M. (2009). Fixed-time artificial insemination with estradiol and progesterone for Bos indicus cows II: Strategies and factors affecting fertility. Theriogenology, 72(2), 210-218.

Sauls-Hiesterman, J. A., Voelz, B. E., & Stevenson, J. S. (2020). A shortened resynchronization treatment for dairy cows after a nonpregnancy diagnosis. Theriogenology, 141(1), 105-112.

Published

22/05/2021

How to Cite

MEDEIROS, M. N. de .; CÂMARA, F. V. .; SOUZA, G. B. de .; MEDEIROS, A. L. de .; MAGALHÃES PADILHA, D. de M. . Hormone protocol strategy based on the anticipation of PGF2α to improve the reproductive efficiency of fixed time embryo transfer (FTET) in dairy cattle. Research, Society and Development, [S. l.], v. 10, n. 6, p. e10510615382, 2021. DOI: 10.33448/rsd-v10i6.15382. Disponível em: https://rsdjournal.org/index.php/rsd/article/view/15382. Acesso em: 17 apr. 2024.

Issue

Section

Agrarian and Biological Sciences