A vacinação profilática com células dendríticas na resposta imune antitumoral e no crescimento do tumor em um modelo de camundongo com câncer de mama
DOI:
https://doi.org/10.33448/rsd-v10i13.20905Palavras-chave:
Câncer; Células Dedríticas; Vacinação; Imunoterapia; Imunidade tumoral.Resumo
As vacinas de células dendríticas têm demonstrado resultados promissores para tumores pouco imunogênicos, podendo promover a geração de melhores respostas imunológicas no microambiente tumoral. Portanto, este estudo avalia a influência da vacinação profilática de células dendríticas na resposta imune antitumoral no microambiente tumoral e no crescimento tumoral, em modelo experimental com câncer de mama induzido por 4T1. Portanto, camundongos Balb/c foram separados em um grupo vacinado e um grupo não vacinado. A vacina de células dendríticas foi diferenciada e maturada ex vivo da medula óssea. Durante o período experimental, os volumes do tumor foram verificados periodicamente. Os tumores foram avaliados quanto à células imunes (linfócitos T auxiliares e linfócitos T citotóxicos), perfil T auxiliar (Th1, Th2, Th17 e Treg), síntese de TNF-α e IFN-γ por Th1 e linfócitos T citotóxicos. O grupo previamente vacinado apresentou diminuição do volume tumoral (14.0, 0-131.7) em comparação ao grupo não vacinado (89.59, 0.1250-459.6) (p = 0.0421). Os subtipos Th1, Th2, Th17, Treg, T citotóxico, incluindo o TNF-α e IFN-γ produzidos por Th1 e T citotóxicos, apresentaram aumento significativo no grupo vacinado, tanto quanto o balanço de Th1/Th2 e Th1/Treg. Os resultados demonstraram que a vacinação profilática com células dendríticas demonstrou considerável efeito antitumoral no modelo estudado, promovendo aumento da ativação de células importantes na resposta imune e redução do volume tumoral. Os dados fornecem evidências de uma ativação oportuna da vigilância imunológica na ausência de carga tumoral.
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