Antifungal activity, antibiofilm and synergic effect of diallyl disulfide and diallyl trisulfide against Candida albicans

Rafael Pereira; Guilherme Mendes  Prado; Raquel Oliveira dos Santos  Fontenelle; Francisco César Barroso  Barbosa; Hélcio Silva dos  Santos; Emmanuel Silva  Marinho; Márcia Machado  Marinho; Selene Maia de  Morais

doi:10.33448/rsd-v11i4.27538

Autores

Rafael Pereira Federal University of Ceará https://orcid.org/0000-0002-6495-2671
Guilherme Mendes Prado Federal University of Ceará https://orcid.org/0000-0002-5641-6983
Raquel Oliveira dos Santos Fontenelle Vale do Acaraú State University https://orcid.org/0000-0002-8865-5954
Francisco César Barroso Barbosa Federal University of Ceará https://orcid.org/0000-0002-3444-6997
Hélcio Silva dos Santos Vale do Acaraú State University https://orcid.org/0000-0001-5527-164X
Emmanuel Silva Marinho Ceará State University https://orcid.org/0000-0002-4774-8775
Márcia Machado Marinho Ceará State University https://orcid.org/0000-0002-7640-2220
Selene Maia de Morais Ceará State University https://orcid.org/0000-0002-2766-3790

DOI:

https://doi.org/10.33448/rsd-v11i4.27538

Palavras-chave:

Candidíase; Antifúngico; Biofilme; Allium sativum; In sílico.

Resumo

O objetivo do presente estudo foi determinar a atividade antifúngica, antibiofilme e efeito sinérgico dos compostos dissulfeto de dialila e trissulfeto de dialila em combinação com agentes antifúngicos contra isolados clínicos de Candida albicans, incluindo também um estudo computacional. Testes de sensibilidade antimicrobiana foram realizados pelo método de microdiluição em caldo para determinar a concentração inibitória mínima contra cepas de C. albicans e a atividade modulatória usando a técnica checkerboard. A formação de biofilme foi avaliada pela quantificação da biomassa pelo método de coloração por cristal violeta. Para o estudo de simulações computacionais de encaixe molecular. Os constituintes apresentaram atividade antifúngica relevante contra cepas de C. albicans. No ensaio de atividade modulatória, demonstrou interação sinérgica com fluconazol e anfotericina B, com aumento de sua ação antifúngica. Os ligantes dissulfeto de dialila, trissulfeto de dialila e fluconazol formaram complexos com a enzima ALS3. Então, ambos os compostos foram considerados produtos promissores para o desenvolvimento de novos medicamentos para prevenir a candidíase.

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Atividade antifúngica, antibiofilme e efeito sinérgico do dissulfeto de dialila e trissulfeto de dialila frente a espécie Candida albicans

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